Strain Name:
C57BL/6J-MtgxR1661Btlr/Mmmh
Stock Number:
039697-MU
Citation ID:
RRID:MMRRC_039697-MU
Other Names:
R1661 (G1), C57BL/6J-MtgxR1661Btlr
Major Collection:

Gene Information

Gene Symbol: Htr4 [MGI:109246] (Mus musculus (mouse))
Name: 5 hydroxytryptamine (serotonin) receptor 4
Synonyms: 5-HT4L, 5-HT4
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Rc3h1 [MGI:2685397] (Mus musculus (mouse))
Name: RING CCCH (C3H) domains 1
Synonyms: 5730557L09Rik, roquin
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Pkp4 [MGI:109281] (Mus musculus (mouse))
Name: plakophilin 4
Synonyms: Armrp, 5031422I09Rik, 9430019K17Rik, p0071
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Mfn1 [MGI:1914664] (Mus musculus (mouse))
Name: mitofusin 1
Synonyms: 2310002F04Rik, HR2, 6330416C07Rik, D3Ertd265e
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Lrig3 [MGI:2443955] (Mus musculus (mouse))
Name: leucine-rich repeats and immunoglobulin-like domains 3
Synonyms: 9130004I02Rik, 9430095K15Rik, 9030421L11Rik
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Map1b [MGI:1306778] (Mus musculus (mouse))
Name: microtubule-associated protein 1B
Synonyms: Mtap-5, LC1, Mtap1b, MAP5, Mtap5
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Timmdc1 [MGI:1922139] (Mus musculus (mouse))
Name: translocase of inner mitochondrial membrane domain containing 1
Synonyms: 2810021C21Rik, 4930455C21Rik
Chromosome: 16
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 39,623,204 bp
  • T to A, chromosome 1 at 69,538,814 bp
  • T to A, chromosome 1 at 127,817,656 bp
  • C to A, chromosome 1 at 134,747,620 bp
  • T to C, chromosome 1 at 152,533,575 bp
  • T to A, chromosome 1 at 160,959,423 bp
  • T to C, chromosome 1 at 190,937,467 bp
  • T to C, chromosome 2 at 25,579,155 bp
  • T to C, chromosome 2 at 59,310,303 bp
  • C to A, chromosome 2 at 76,830,856 bp
  • C to T, chromosome 2 at 110,733,898 bp
  • T to C, chromosome 2 at 111,306,771 bp
  • T to C, chromosome 2 at 111,408,753 bp
  • T to C, chromosome 2 at 146,035,319 bp
  • T to G, chromosome 3 at 32,534,322 bp
  • A to C, chromosome 3 at 32,590,039 bp
  • A to G, chromosome 3 at 79,515,149 bp
  • C to T, chromosome 3 at 96,651,738 bp
  • T to C, chromosome 3 at 135,594,957 bp
  • T to C, chromosome 4 at 42,970,215 bp
  • T to A, chromosome 4 at 129,657,471 bp
  • C to T, chromosome 4 at 151,985,704 bp
  • A to T, chromosome 5 at 35,603,027 bp
  • A to T, chromosome 5 at 96,598,909 bp
  • T to A, chromosome 5 at 118,749,748 bp
  • A to T, chromosome 6 at 18,434,983 bp
  • G to C, chromosome 6 at 29,285,938 bp
  • T to A, chromosome 6 at 52,736,912 bp
  • A to T, chromosome 6 at 57,978,461 bp
  • T to C, chromosome 6 at 73,124,778 bp
  • A to G, chromosome 6 at 86,628,736 bp
  • G to A, chromosome 6 at 89,651,485 bp
  • G to T, chromosome 6 at 92,880,623 bp
  • A to G, chromosome 6 at 108,482,897 bp
  • A to G, chromosome 7 at 25,363,847 bp
  • A to T, chromosome 7 at 29,101,738 bp
  • A to G, chromosome 7 at 30,316,222 bp
  • A to C, chromosome 7 at 30,532,323 bp
  • A to G, chromosome 7 at 35,417,680 bp
  • A to T, chromosome 7 at 46,692,800 bp
  • G to T, chromosome 7 at 90,475,971 bp
  • T to C, chromosome 7 at 107,074,274 bp
  • A to G, chromosome 8 at 13,035,209 bp
  • G to T, chromosome 8 at 70,078,930 bp
  • T to C, chromosome 8 at 79,372,029 bp
  • G to A, chromosome 8 at 104,737,555 bp
  • A to G, chromosome 8 at 111,479,362 bp
  • T to C, chromosome 8 at 124,542,890 bp
  • C to T, chromosome 9 at 19,537,328 bp
  • A to G, chromosome 9 at 48,604,874 bp
  • C to T, chromosome 9 at 62,797,274 bp
  • T to A, chromosome 9 at 102,730,096 bp
  • A to G, chromosome 10 at 41,514,180 bp
  • T to C, chromosome 10 at 125,997,701 bp
  • A to G, chromosome 11 at 4,919,792 bp
  • C to T, chromosome 11 at 70,728,397 bp
  • A to G, chromosome 11 at 78,155,161 bp
  • T to C, chromosome 11 at 85,210,110 bp
  • A to T, chromosome 11 at 107,491,906 bp
  • A to T, chromosome 11 at 118,121,495 bp
  • GTACATACATACATACATACATACATACA to GTACATACATACATACATACATACATACATACA, chromosome 12 at 40,185,261 bp
  • T to A, chromosome 12 at 70,255,113 bp
  • A to T, chromosome 12 at 100,149,860 bp
  • T to C, chromosome 12 at 110,656,357 bp
  • C to T, chromosome 13 at 3,573,860 bp
  • C to A, chromosome 13 at 55,631,971 bp
  • T to A, chromosome 13 at 99,431,929 bp
  • A to G, chromosome 13 at 100,693,573 bp
  • T to C, chromosome 14 at 28,518,343 bp
  • A to T, chromosome 14 at 59,280,788 bp
  • T to C, chromosome 14 at 59,860,186 bp
  • A to T, chromosome 14 at 108,911,927 bp
  • A to C, chromosome 15 at 58,191,469 bp
  • G to A, chromosome 15 at 79,620,677 bp
  • A to G, chromosome 15 at 89,253,051 bp
  • A to G, chromosome 15 at 97,384,713 bp
  • A to T, chromosome 15 at 99,707,638 bp
  • C to A, chromosome 16 at 17,776,488 bp
  • A to C, chromosome 16 at 38,510,717 bp
  • A to G, chromosome 17 at 8,898,870 bp
  • A to G, chromosome 17 at 21,509,504 bp
  • G to A, chromosome 17 at 24,377,842 bp
  • A to G, chromosome 17 at 73,188,004 bp
  • A to T, chromosome 17 at 74,021,481 bp
  • T to A, chromosome 18 at 34,628,591 bp
  • G to A, chromosome 18 at 50,160,215 bp
  • T to C, chromosome 18 at 62,412,234 bp
  • C to A, chromosome 18 at 77,185,570 bp
  • T to C, chromosome 18 at 89,042,129 bp
  • A to G, chromosome 19 at 3,267,246 bp
  • A to T, chromosome 19 at 10,629,080 bp
  • A to T, chromosome 19 at 17,447,574 bp
  • A to T, chromosome 19 at 47,278,320 bp
  • A to G, chromosome 19 at 53,625,065 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1661 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039697-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.