Strain Name:
C57BL/6J-MtgxR1725Btlr/Mmmh
Stock Number:
039757-MU
Citation ID:
RRID:MMRRC_039757-MU
Other Names:
R1725 (G1), C57BL/6J-MtgxR1725Btlr
Major Collection:

Gene Information

Gene Symbol: Col2a1 [MGI:88452] (Mus musculus (mouse))
Name: collagen, type II, alpha 1
Synonyms: Del1, M100413, Rgsc413, Col2a-1, M100856, Col2, Rgsc856, Lpk, Col2a
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Sulf2 [MGI:1919293] (Mus musculus (mouse))
Name: sulfatase 2
Synonyms: 2010004N24Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Utrn [MGI:104631] (Mus musculus (mouse))
Name: utrophin
Synonyms: DRP, G-utrophin, Dmdl
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Ptk2 [MGI:95481] (Mus musculus (mouse))
Name: PTK2 protein tyrosine kinase 2
Synonyms: Fadk, FAK, FRNK
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Espl1 [MGI:2146156] (Mus musculus (mouse))
Name: extra spindle pole bodies 1, separase
Synonyms: PRCE, PRCE, Cerp, separase, ESP1, SSE
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Snx1 [MGI:1928395] (Mus musculus (mouse))
Name: sorting nexin 1
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Tet1 [MGI:1098693] (Mus musculus (mouse))
Name: tet methylcytosine dioxygenase 1
Synonyms: BB001228, Cxxc6, 2510010B09Rik, D10Ertd17e
Chromosome: 10
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 25,826,300 bp
  • A to G, chromosome 1 at 53,468,821 bp
  • T to C, chromosome 1 at 131,816,058 bp
  • C to T, chromosome 1 at 136,098,623 bp
  • T to A, chromosome 1 at 140,354,443 bp
  • A to G, chromosome 1 at 163,964,838 bp
  • C to T, chromosome 1 at 172,957,341 bp
  • T to A, chromosome 1 at 185,406,992 bp
  • T to C, chromosome 1 at 189,680,479 bp
  • C to A, chromosome 2 at 30,350,667 bp
  • T to C, chromosome 2 at 76,862,383 bp
  • T to A, chromosome 2 at 85,038,343 bp
  • T to C, chromosome 2 at 110,731,246 bp
  • C to T, chromosome 2 at 121,252,000 bp
  • T to C, chromosome 2 at 150,686,200 bp
  • T to C, chromosome 2 at 152,257,972 bp
  • A to G, chromosome 2 at 155,556,844 bp
  • A to G, chromosome 2 at 165,724,685 bp
  • T to C, chromosome 2 at 166,081,361 bp
  • A to T, chromosome 2 at 166,601,736 bp
  • A to G, chromosome 2 at 173,006,961 bp
  • T to G, chromosome 3 at 62,506,939 bp
  • A to G, chromosome 3 at 72,891,761 bp
  • C to A, chromosome 3 at 135,667,758 bp
  • C to A, chromosome 4 at 11,804,125 bp
  • A to T, chromosome 4 at 63,144,276 bp
  • G to T, chromosome 4 at 103,284,384 bp
  • G to A, chromosome 4 at 136,659,778 bp
  • T to A, chromosome 4 at 137,633,422 bp
  • A to T, chromosome 4 at 145,143,260 bp
  • T to G, chromosome 4 at 148,500,012 bp
  • T to C, chromosome 4 at 154,148,752 bp
  • T to C, chromosome 5 at 35,592,172 bp
  • G to A, chromosome 5 at 38,135,255 bp
  • A to T, chromosome 5 at 87,411,871 bp
  • T to C, chromosome 5 at 125,482,935 bp
  • C to T, chromosome 5 at 142,886,703 bp
  • G to T, chromosome 5 at 148,959,401 bp
  • G to A, chromosome 6 at 83,035,593 bp
  • G to A, chromosome 6 at 85,901,098 bp
  • G to A, chromosome 6 at 125,646,282 bp
  • A to G, chromosome 6 at 147,592,043 bp
  • A to T, chromosome 7 at 12,770,857 bp
  • A to T, chromosome 7 at 18,428,011 bp
  • A to G, chromosome 7 at 18,840,068 bp
  • A to G, chromosome 7 at 22,790,647 bp
  • C to A, chromosome 7 at 45,992,357 bp
  • T to C, chromosome 7 at 83,604,732 bp
  • A to T, chromosome 7 at 106,714,058 bp
  • T to C, chromosome 8 at 124,728,452 bp
  • T to G, chromosome 9 at 49,458,115 bp
  • A to G, chromosome 9 at 66,098,329 bp
  • G to A, chromosome 10 at 4,353,942 bp
  • T to C, chromosome 10 at 12,663,519 bp
  • G to A, chromosome 10 at 14,716,424 bp
  • T to A, chromosome 10 at 33,779,709 bp
  • G to A, chromosome 10 at 58,304,115 bp
  • A to G, chromosome 10 at 62,814,477 bp
  • T to A, chromosome 10 at 77,041,838 bp
  • A to T, chromosome 10 at 109,823,590 bp
  • A to G, chromosome 10 at 128,269,811 bp
  • A to C, chromosome 11 at 62,820,505 bp
  • T to C, chromosome 11 at 70,465,093 bp
  • A to G, chromosome 11 at 76,748,585 bp
  • TC to T, chromosome 11 at 77,786,002 bp
  • T to G, chromosome 11 at 103,201,712 bp
  • G to A, chromosome 13 at 52,835,496 bp
  • T to C, chromosome 13 at 54,526,406 bp
  • T to G, chromosome 14 at 14,328,977 bp
  • A to G, chromosome 14 at 30,866,758 bp
  • A to T, chromosome 14 at 52,232,573 bp
  • A to G, chromosome 14 at 86,966,323 bp
  • A to C, chromosome 15 at 47,596,807 bp
  • A to T, chromosome 15 at 52,333,604 bp
  • A to G, chromosome 15 at 73,242,406 bp
  • A to T, chromosome 15 at 75,235,650 bp
  • T to G, chromosome 15 at 80,692,267 bp
  • T to C, chromosome 15 at 97,991,402 bp
  • T to C, chromosome 15 at 98,845,234 bp
  • A to T, chromosome 15 at 101,692,557 bp
  • G to T, chromosome 15 at 102,313,221 bp
  • A to T, chromosome 16 at 88,627,275 bp
  • T to A, chromosome 17 at 18,929,135 bp
  • A to G, chromosome 17 at 32,977,337 bp
  • T to C, chromosome 17 at 33,642,066 bp
  • A to G, chromosome 17 at 56,106,473 bp
  • T to C, chromosome 18 at 34,744,067 bp
  • T to A, chromosome 18 at 37,520,188 bp
  • T to A, chromosome 18 at 77,293,241 bp
  • T to C, chromosome 19 at 13,502,519 bp
  • T to G, chromosome 19 at 58,792,762 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1725 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039757-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.