Strain Name:
C57BL/6J-MtgxR1726Btlr/Mmmh
Stock Number:
039758-MU
Citation ID:
RRID:MMRRC_039758-MU
Other Names:
R1726 (G1), C57BL/6J-MtgxR1726Btlr
Major Collection:

Gene Information

Gene Symbol: Pkd1 [MGI:97603] (Mus musculus (mouse))
Name: polycystin 1, transient receptor poteintial channel interacting
Synonyms: polycystin-1, PC1, PC-1
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Slc12a6 [MGI:2135960] (Mus musculus (mouse))
Name: solute carrier family 12, member 6
Synonyms: gaxp, KCC3
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Kmt2c [MGI:2444959] (Mus musculus (mouse))
Name: lysine (K)-specific methyltransferase 2C
Synonyms: Mll3, HALR, E330008K23Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Chrnb2 [MGI:87891] (Mus musculus (mouse))
Name: cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal)
Synonyms: C030030P04Rik, Acrb-2, [b]2-nAchR, Acrb2
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Dgat2 [MGI:1915050] (Mus musculus (mouse))
Name: diacylglycerol O-acyltransferase 2
Synonyms: 0610010B06Rik
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Dip2c [MGI:1920179] (Mus musculus (mouse))
Name: disco interacting protein 2 homolog C
Synonyms: 2900024P20Rik
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Arhgef18 [MGI:2142567] (Mus musculus (mouse))
Name: rho/rac guanine nucleotide exchange factor (GEF) 18
Synonyms: D030053O22Rik
Chromosome: 8
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 52,637,395 bp
  • C to A, chromosome 1 at 69,548,688 bp
  • A to G, chromosome 1 at 172,957,091 bp
  • A to G, chromosome 2 at 77,108,356 bp
  • C to A, chromosome 2 at 91,059,140 bp
  • T to A, chromosome 2 at 112,347,426 bp
  • G to A, chromosome 2 at 127,880,446 bp
  • A to T, chromosome 2 at 128,967,215 bp
  • G to T, chromosome 2 at 164,771,317 bp
  • A to T, chromosome 3 at 89,761,202 bp
  • T to C, chromosome 3 at 106,389,843 bp
  • A to G, chromosome 3 at 108,467,868 bp
  • A to G, chromosome 3 at 141,818,103 bp
  • A to G, chromosome 4 at 34,954,028 bp
  • G to A, chromosome 4 at 41,756,001 bp
  • G to T, chromosome 4 at 155,803,753 bp
  • C to T, chromosome 5 at 25,315,005 bp
  • T to C, chromosome 5 at 31,169,123 bp
  • T to C, chromosome 5 at 96,223,827 bp
  • C to T, chromosome 5 at 108,673,675 bp
  • C to A, chromosome 5 at 148,959,501 bp
  • T to C, chromosome 6 at 40,998,026 bp
  • T to C, chromosome 6 at 100,637,035 bp
  • A to G, chromosome 7 at 99,182,416 bp
  • T to C, chromosome 7 at 108,755,008 bp
  • T to A, chromosome 7 at 123,489,823 bp
  • T to C, chromosome 7 at 134,899,138 bp
  • A to T, chromosome 8 at 3,454,228 bp
  • A to G, chromosome 10 at 19,356,734 bp
  • G to A, chromosome 10 at 53,537,881 bp
  • C to A, chromosome 10 at 75,992,527 bp
  • T to C, chromosome 10 at 80,335,120 bp
  • A to C, chromosome 10 at 81,155,855 bp
  • A to T, chromosome 11 at 69,497,889 bp
  • A to T, chromosome 11 at 70,009,461 bp
  • C to A, chromosome 11 at 77,467,738 bp
  • TC to T, chromosome 11 at 77,786,002 bp
  • A to G, chromosome 11 at 82,061,720 bp
  • A to T, chromosome 11 at 86,064,914 bp
  • G to T, chromosome 11 at 87,080,613 bp
  • A to G, chromosome 11 at 97,224,669 bp
  • A to G, chromosome 11 at 102,310,917 bp
  • A to G, chromosome 11 at 116,145,454 bp
  • G to C, chromosome 12 at 118,874,801 bp
  • A to T, chromosome 12 at 118,907,532 bp
  • A to G, chromosome 13 at 9,575,428 bp
  • A to T, chromosome 13 at 23,905,591 bp
  • T to A, chromosome 13 at 32,729,169 bp
  • A to T, chromosome 13 at 67,474,720 bp
  • C to T, chromosome 13 at 98,316,296 bp
  • A to T, chromosome 14 at 50,361,541 bp
  • T to A, chromosome 14 at 50,516,179 bp
  • T to C, chromosome 14 at 54,469,656 bp
  • A to G, chromosome 14 at 57,826,635 bp
  • A to G, chromosome 14 at 63,537,098 bp
  • A to T, chromosome 14 at 123,308,404 bp
  • A to G, chromosome 15 at 89,557,986 bp
  • A to C, chromosome 16 at 13,838,300 bp
  • A to T, chromosome 16 at 45,096,005 bp
  • C to T, chromosome 17 at 24,564,176 bp
  • T to A, chromosome 17 at 27,111,690 bp
  • C to A, chromosome 17 at 27,886,251 bp
  • T to A, chromosome 17 at 36,015,621 bp
  • T to C, chromosome 17 at 67,042,327 bp
  • C to A, chromosome 18 at 21,148,262 bp
  • T to A, chromosome 18 at 37,449,594 bp
  • T to C, chromosome 18 at 61,697,778 bp
  • T to A, chromosome 18 at 78,116,466 bp
  • A to G, chromosome Y at 10,616,358 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1726 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039758-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.