Strain Name:
C57BL/6J-MtgxR1754Btlr/Mmmh
Stock Number:
039786-MU
Citation ID:
RRID:MMRRC_039786-MU
Other Names:
R1754 (G1), C57BL/6J-MtgxR1754Btlr
Major Collection:

Gene Information

Gene Symbol: Ppp5c [MGI:102666] (Mus musculus (mouse))
Name: protein phosphatase 5, catalytic subunit
Synonyms: ANP receptor, PP5
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Polr3e [MGI:1349452] (Mus musculus (mouse))
Name: polymerase (RNA) III (DNA directed) polypeptide E
Synonyms: Sin, RPC5
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Gabra6 [MGI:95618] (Mus musculus (mouse))
Name: gamma-aminobutyric acid (GABA) A receptor, subunit alpha 6
Synonyms: Gabra-6, GABA-ARalpha6, alpha6
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Vcan [MGI:102889] (Mus musculus (mouse))
Name: versican
Synonyms: Cspg2, DPEAAE, heart defect, PG-M, 5430420N07Rik, hdf
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Cd14 [MGI:88318] (Mus musculus (mouse))
Name: CD14 antigen
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Avpr1b [MGI:1347010] (Mus musculus (mouse))
Name: arginine vasopressin receptor 1B
Synonyms: V3/V1b pituitary vasopressin receptor, VPR3, V1BR, AVPR3, V3/V1b, V1bR
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Kcns2 [MGI:1197011] (Mus musculus (mouse))
Name: K+ voltage-gated channel, subfamily S, 2
Synonyms: Kv9.2, E130006J24Rik
Chromosome: 15
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 53,504,185 bp
  • A to C, chromosome 1 at 53,561,900 bp
  • T to C, chromosome 1 at 66,473,221 bp
  • T to C, chromosome 1 at 131,600,101 bp
  • T to C, chromosome 1 at 171,217,394 bp
  • A to G, chromosome 1 at 174,100,033 bp
  • T to A, chromosome 2 at 25,434,333 bp
  • C to A, chromosome 2 at 76,751,040 bp
  • A to T, chromosome 2 at 88,525,815 bp
  • G to T, chromosome 2 at 94,303,691 bp
  • G to A, chromosome 2 at 120,533,763 bp
  • A to C, chromosome 2 at 120,533,764 bp
  • T to C, chromosome 3 at 10,182,224 bp
  • T to C, chromosome 3 at 29,668,333 bp
  • T to A, chromosome 3 at 32,718,574 bp
  • A to G, chromosome 3 at 32,730,084 bp
  • T to C, chromosome 3 at 37,225,525 bp
  • C to G, chromosome 3 at 136,881,448 bp
  • A to G, chromosome 4 at 11,583,906 bp
  • T to G, chromosome 4 at 33,329,634 bp
  • T to A, chromosome 4 at 49,529,342 bp
  • A to T, chromosome 4 at 90,223,809 bp
  • A to G, chromosome 4 at 132,232,027 bp
  • T to G, chromosome 5 at 76,560,322 bp
  • T to C, chromosome 5 at 105,812,657 bp
  • A to T, chromosome 5 at 113,955,845 bp
  • A to C, chromosome 5 at 115,245,865 bp
  • A to T, chromosome 5 at 121,607,481 bp
  • T to C, chromosome 5 at 150,481,590 bp
  • G to T, chromosome 6 at 3,373,126 bp
  • G to A, chromosome 6 at 4,518,822 bp
  • T to C, chromosome 6 at 38,067,829 bp
  • TA to TAA, chromosome 6 at 66,716,533 bp
  • A to G, chromosome 6 at 77,636,749 bp
  • A to T, chromosome 6 at 128,357,859 bp
  • G to T, chromosome 7 at 10,099,946 bp
  • T to C, chromosome 7 at 17,005,310 bp
  • C to T, chromosome 7 at 29,260,366 bp
  • T to C, chromosome 7 at 44,255,004 bp
  • T to C, chromosome 7 at 103,418,848 bp
  • T to C, chromosome 7 at 115,477,055 bp
  • T to C, chromosome 7 at 120,939,298 bp
  • A to T, chromosome 7 at 140,466,860 bp
  • A to G, chromosome 8 at 25,570,210 bp
  • A to G, chromosome 8 at 69,101,961 bp
  • T to C, chromosome 8 at 71,490,978 bp
  • G to T, chromosome 8 at 71,623,179 bp
  • A to G, chromosome 8 at 71,711,246 bp
  • A to T, chromosome 8 at 81,770,811 bp
  • A to G, chromosome 8 at 83,669,297 bp
  • CAT to C, chromosome 8 at 105,941,814 bp
  • A to T, chromosome 8 at 117,030,719 bp
  • T to A, chromosome 8 at 124,877,006 bp
  • T to G, chromosome 9 at 46,999,049 bp
  • A to T, chromosome 9 at 102,541,927 bp
  • T to A, chromosome 9 at 111,392,871 bp
  • A to T, chromosome 10 at 20,407,219 bp
  • T to C, chromosome 10 at 59,427,940 bp
  • T to A, chromosome 10 at 78,030,290 bp
  • T to A, chromosome 10 at 78,955,697 bp
  • T to C, chromosome 10 at 127,370,293 bp
  • G to A, chromosome 10 at 130,163,164 bp
  • A to C, chromosome 11 at 24,164,724 bp
  • A to G, chromosome 11 at 42,316,561 bp
  • T to C, chromosome 11 at 54,055,873 bp
  • A to T, chromosome 11 at 58,455,262 bp
  • A to G, chromosome 11 at 59,558,402 bp
  • C to A, chromosome 11 at 68,813,058 bp
  • A to G, chromosome 11 at 78,337,847 bp
  • T to C, chromosome 12 at 110,855,457 bp
  • T to C, chromosome 13 at 23,460,468 bp
  • A to T, chromosome 13 at 50,701,087 bp
  • A to G, chromosome 13 at 55,013,424 bp
  • A to G, chromosome 13 at 89,704,735 bp
  • A to T, chromosome 13 at 113,216,696 bp
  • A to G, chromosome 14 at 60,284,907 bp
  • A to G, chromosome 14 at 60,379,757 bp
  • T to C, chromosome 14 at 111,680,519 bp
  • C to A, chromosome 15 at 6,735,368 bp
  • T to C, chromosome 15 at 34,839,517 bp
  • A to T, chromosome 15 at 77,735,762 bp
  • A to G, chromosome 15 at 80,249,602 bp
  • A to G, chromosome 15 at 80,371,529 bp
  • A to G, chromosome 16 at 32,299,124 bp
  • T to A, chromosome 16 at 45,589,509 bp
  • T to A, chromosome 16 at 59,110,581 bp
  • G to A, chromosome 16 at 73,973,798 bp
  • A to G, chromosome 16 at 92,321,031 bp
  • A to G, chromosome 17 at 5,279,201 bp
  • G to A, chromosome 17 at 24,377,779 bp
  • T to G, chromosome 17 at 28,821,323 bp
  • T to C, chromosome 17 at 32,775,334 bp
  • C to T, chromosome 17 at 34,051,813 bp
  • T to C, chromosome 17 at 66,380,183 bp
  • G to A, chromosome 17 at 71,403,173 bp
  • A to G, chromosome 18 at 32,123,830 bp
  • A to T, chromosome 18 at 36,725,514 bp
  • T to C, chromosome 19 at 3,968,517 bp
  • T to C, chromosome 19 at 13,365,353 bp
  • A to T, chromosome 19 at 44,155,601 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1754 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039786-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.