MMRRC:039962-MU

G to A, chromosome 1 at 44,061,849 bp
T to C, chromosome 1 at 127,823,122 bp
T to C, chromosome 1 at 151,696,228 bp
T to C, chromosome 1 at 171,461,891 bp
A to T, chromosome 1 at 175,153,203 bp
T to A, chromosome 2 at 11,783,632 bp
T to A, chromosome 2 at 13,278,538 bp
A to G, chromosome 2 at 44,752,186 bp
A to T, chromosome 2 at 52,228,850 bp
T to A, chromosome 2 at 61,812,256 bp
A to T, chromosome 2 at 70,973,279 bp
T to C, chromosome 2 at 71,845,961 bp
A to T, chromosome 2 at 74,706,712 bp
C to T, chromosome 2 at 119,334,169 bp
A to T, chromosome 2 at 122,346,520 bp
A to G, chromosome 2 at 130,029,969 bp
A to G, chromosome 2 at 180,177,419 bp
C to T, chromosome 3 at 107,331,552 bp
T to A, chromosome 3 at 130,418,829 bp
T to C, chromosome 4 at 88,423,066 bp
A to G, chromosome 4 at 107,306,780 bp
T to C, chromosome 4 at 123,370,666 bp
A to G, chromosome 4 at 126,353,727 bp
T to C, chromosome 4 at 147,865,965 bp
A to G, chromosome 4 at 151,056,160 bp
C to G, chromosome 4 at 154,256,066 bp
A to T, chromosome 5 at 8,930,796 bp
T to A, chromosome 5 at 35,097,630 bp
A to G, chromosome 5 at 36,602,814 bp
A to G, chromosome 5 at 36,816,180 bp
G to A, chromosome 5 at 66,999,099 bp
G to A, chromosome 5 at 88,526,234 bp
A to G, chromosome 5 at 127,783,764 bp
G to A, chromosome 5 at 145,132,916 bp
G to A, chromosome 6 at 17,097,593 bp
T to C, chromosome 6 at 23,599,480 bp
T to C, chromosome 6 at 34,872,812 bp
G to C, chromosome 6 at 63,909,061 bp
T to A, chromosome 6 at 84,195,331 bp
A to G, chromosome 6 at 98,248,190 bp
A to G, chromosome 6 at 118,606,266 bp
T to C, chromosome 6 at 130,018,945 bp
T to C, chromosome 6 at 141,839,550 bp
G to A, chromosome 7 at 12,903,840 bp
G to T, chromosome 7 at 27,227,659 bp
A to T, chromosome 7 at 34,118,938 bp
G to T, chromosome 7 at 44,304,674 bp
A to G, chromosome 7 at 84,343,751 bp
T to C, chromosome 7 at 101,579,946 bp
A to G, chromosome 7 at 127,260,514 bp
A to T, chromosome 8 at 65,001,312 bp
A to G, chromosome 8 at 85,010,761 bp
A to G, chromosome 8 at 85,517,996 bp
G to A, chromosome 8 at 95,007,300 bp
A to G, chromosome 8 at 105,707,576 bp
C to A, chromosome 8 at 111,689,519 bp
A to T, chromosome 9 at 7,001,377 bp
G to T, chromosome 9 at 57,765,186 bp
T to G, chromosome 9 at 59,330,415 bp
A to T, chromosome 9 at 67,886,276 bp
G to A, chromosome 9 at 105,709,384 bp
G to A, chromosome 9 at 108,960,010 bp
T to A, chromosome 9 at 109,071,867 bp
A to T, chromosome 10 at 13,239,979 bp
T to C, chromosome 10 at 42,572,778 bp
T to G, chromosome 10 at 79,293,737 bp
A to T, chromosome 10 at 99,022,931 bp
A to T, chromosome 10 at 107,582,388 bp
T to C, chromosome 10 at 109,716,530 bp
T to A, chromosome 11 at 6,214,588 bp
T to C, chromosome 11 at 9,038,296 bp
A to G, chromosome 11 at 54,323,370 bp
A to G, chromosome 11 at 54,987,045 bp
T to C, chromosome 11 at 59,090,791 bp
T to C, chromosome 11 at 60,502,083 bp
T to C, chromosome 11 at 61,154,517 bp
A to T, chromosome 11 at 67,746,792 bp
A to T, chromosome 11 at 68,829,920 bp
A to T, chromosome 11 at 70,688,977 bp
A to G, chromosome 11 at 74,630,009 bp
G to T, chromosome 11 at 83,769,220 bp
T to A, chromosome 11 at 87,484,687 bp
T to C, chromosome 11 at 97,711,155 bp
A to C, chromosome 11 at 99,519,823 bp
T to C, chromosome 11 at 100,012,709 bp
T to A, chromosome 11 at 100,084,844 bp
A to G, chromosome 11 at 106,421,950 bp
GTACATACATACATACATACATACATACA to GTACATACATACATACATACATACATACATACA, chromosome 12 at 40,185,261 bp
T to C, chromosome 12 at 76,074,544 bp
A to T, chromosome 12 at 102,401,924 bp
A to T, chromosome 13 at 21,703,117 bp
T to C, chromosome 13 at 55,676,120 bp
C to T, chromosome 13 at 70,791,886 bp
A to G, chromosome 13 at 74,646,639 bp
T to A, chromosome 13 at 76,123,554 bp
A to T, chromosome 13 at 81,510,911 bp
A to T, chromosome 13 at 100,074,381 bp
G to T, chromosome 14 at 55,074,732 bp
G to T, chromosome 14 at 55,496,317 bp
T to C, chromosome 14 at 55,498,630 bp
T to C, chromosome 14 at 62,693,640 bp
A to T, chromosome 14 at 67,757,135 bp
T to A, chromosome 14 at 103,229,404 bp
A to G, chromosome 15 at 76,694,294 bp
T to C, chromosome 15 at 81,611,537 bp
A to G, chromosome 15 at 91,856,646 bp
G to A, chromosome 15 at 101,548,535 bp
T to A, chromosome 16 at 20,719,046 bp
T to A, chromosome 16 at 23,927,566 bp
A to G, chromosome 17 at 18,940,238 bp
A to C, chromosome 17 at 21,261,523 bp
A to G, chromosome 17 at 24,500,771 bp
A to G, chromosome 17 at 35,909,340 bp
A to G, chromosome 17 at 36,058,005 bp
T to C, chromosome 17 at 46,636,627 bp
T to G, chromosome 17 at 74,408,863 bp
T to A, chromosome 18 at 6,332,669 bp
C to A, chromosome 18 at 62,179,413 bp
T to C, chromosome 19 at 5,426,229 bp
C to T, chromosome 19 at 11,649,595 bp
T to A, chromosome 19 at 38,157,519 bp
T to C, chromosome 19 at 44,235,780 bp
T to A, chromosome 19 at 46,308,052 bp
T to C, chromosome 19 at 48,029,224 bp
T to C, chromosome X at 101,441,823 bp
T to A, chromosome X at 160,127,358 bp

Genotype Determination

Genotyping Protocol(s)
Center protocol and contact for technical support will be shipped with mice.

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.

Strain Development

C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.

Suggested Control Mice

Wild-type littermates or C57BL/6J mice

Strain Origin

Donor

Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Primary Reference

MUTAGENETIX record for R1944 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information Expand All

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

Appearance

Coat Color

Black

Breeding

MMRRC Breeding System

Random intra-strain mating

Breeding Scheme(s)

When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.

Overall Breeding Performance

Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size

Undetermined

Recommended wean age

3 weeks

Order Request Information Expand All

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description	Distribution Fee / Unit (US $) Shipping & Handling not included	Units	Notes
039962-MU-RESUS	Litter recovered from cryo-archive	$5,475.00 / Non-Profit	Litter	Recovered litter⁴; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

¹ The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

³ An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

⁴ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

Strain Detail Sheet

Gene Information Expand All

Colony and Husbandry Information Expand All

Order Request Information Expand All