Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR1952Btlr/Mmmh
Stock Number:
039966-MU
Citation ID:
RRID:MMRRC_039966-MU
Other Names:
R1952 (G1), C57BL/6J-MtgxR1952Btlr
Major Collection:

Strain Information

Pax2
Name: paired box 2
Synonyms: Pax-2, Opdc
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18504
HGNC: HGNC:8616
Homologene: 2968
Satb1
Name: special AT-rich sequence binding protein 1
Synonyms: 2610306G12Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20230
Homologene: 2232
Epha7
Name: Eph receptor A7
Synonyms: MDK1, Ebk, Cek11, Ehk3, Hek11, Mdk1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13841
HGNC: HGNC:3390
Homologene: 20935
Pcf11
Name: PCF11 cleavage and polyadenylation factor subunit
Synonyms: 5730417B17Rik, 2500001H09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74737
Homologene: 32282
Camsap3
Name: calmodulin regulated spectrin-associated protein family, member 3
Synonyms: Nezha, 2310057J16Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 69697
Homologene: 18966
Mlst8
Name: MTOR associated protein, LST8 homolog (S. cerevisiae)
Synonyms: 0610033N12Rik, mLST8, Gbl
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 56716
VEGA: 17
Homologene: 6833
Adgrg1
Name: adhesion G protein-coupled receptor G1
Synonyms: Cyt28, Gpr56
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14766
HGNC: HGNC:4512
Homologene: 4156
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 15,860,295 bp
  • A to T, chromosome 1 at 44,017,208 bp
  • T to C, chromosome 1 at 56,899,070 bp
  • T to C, chromosome 1 at 60,234,840 bp
  • A to G, chromosome 1 at 74,687,559 bp
  • G to A, chromosome 1 at 87,126,516 bp
  • A to G, chromosome 2 at 72,208,127 bp
  • G to T, chromosome 2 at 93,859,433 bp
  • T to A, chromosome 2 at 102,853,087 bp
  • G to A, chromosome 2 at 121,359,010 bp
  • G to A, chromosome 2 at 145,928,200 bp
  • T to C, chromosome 2 at 163,498,296 bp
  • T to A, chromosome 3 at 25,436,300 bp
  • A to G, chromosome 3 at 89,849,485 bp
  • T to C, chromosome 3 at 97,666,627 bp
  • A to T, chromosome 3 at 115,101,940 bp
  • T to C, chromosome 4 at 11,221,150 bp
  • A to T, chromosome 4 at 19,841,578 bp
  • A to G, chromosome 4 at 28,950,474 bp
  • A to T, chromosome 4 at 45,458,347 bp
  • T to A, chromosome 4 at 63,132,318 bp
  • T to A, chromosome 4 at 63,283,893 bp
  • C to T, chromosome 4 at 117,265,986 bp
  • C to T, chromosome 4 at 124,851,946 bp
  • A to T, chromosome 5 at 20,998,310 bp
  • A to G, chromosome 6 at 6,152,482 bp
  • A to G, chromosome 6 at 67,292,316 bp
  • T to C, chromosome 6 at 116,255,091 bp
  • A to G, chromosome 6 at 134,432,366 bp
  • A to G, chromosome 7 at 30,131,632 bp
  • G to A, chromosome 7 at 41,044,247 bp
  • A to G, chromosome 7 at 63,650,876 bp
  • A to T, chromosome 7 at 92,661,338 bp
  • A to G, chromosome 7 at 108,924,563 bp
  • A to G, chromosome 8 at 3,604,789 bp
  • G to A, chromosome 8 at 4,523,461 bp
  • T to C, chromosome 8 at 45,033,926 bp
  • T to C, chromosome 8 at 95,008,491 bp
  • T to C, chromosome 9 at 37,429,020 bp
  • T to A, chromosome 9 at 39,078,284 bp
  • T to C, chromosome 9 at 39,820,067 bp
  • C to T, chromosome 9 at 42,046,624 bp
  • T to C, chromosome 9 at 65,369,347 bp
  • A to T, chromosome 9 at 88,476,874 bp
  • T to A, chromosome 9 at 106,370,829 bp
  • G to A, chromosome 9 at 108,513,181 bp
  • T to A, chromosome 10 at 24,049,849 bp
  • T to C, chromosome 10 at 81,595,485 bp
  • C to T, chromosome 10 at 116,822,811 bp
  • A to C, chromosome 10 at 127,567,431 bp
  • A to G, chromosome 10 at 128,209,362 bp
  • T to C, chromosome 10 at 128,225,903 bp
  • A to G, chromosome 11 at 5,977,955 bp
  • G to A, chromosome 11 at 32,214,277 bp
  • A to T, chromosome 11 at 50,406,406 bp
  • T to A, chromosome 11 at 94,097,358 bp
  • T to C, chromosome 11 at 97,677,222 bp
  • A to G, chromosome 11 at 100,352,279 bp
  • G to T, chromosome 11 at 115,441,329 bp
  • C to G, chromosome 11 at 120,595,932 bp
  • T to A, chromosome 12 at 70,030,926 bp
  • A to G, chromosome 13 at 22,088,139 bp
  • G to A, chromosome 13 at 27,270,153 bp
  • C to T, chromosome 13 at 113,366,275 bp
  • A to T, chromosome 14 at 21,837,879 bp
  • A to G, chromosome 14 at 32,388,336 bp
  • T to C, chromosome 14 at 47,270,190 bp
  • T to C, chromosome 15 at 101,224,500 bp
  • T to C, chromosome 15 at 101,847,748 bp
  • A to C, chromosome 16 at 69,857,164 bp
  • A to C, chromosome 17 at 6,728,333 bp
  • AT to ATT, chromosome 17 at 24,478,013 bp
  • C to G, chromosome 17 at 34,193,507 bp
  • T to C, chromosome 17 at 34,200,910 bp
  • T to C, chromosome 17 at 34,244,081 bp
  • A to T, chromosome 17 at 45,642,959 bp
  • A to T, chromosome 17 at 51,740,145 bp
  • T to C, chromosome 17 at 66,031,571 bp
  • A to T, chromosome 17 at 66,940,580 bp
  • A to T, chromosome 18 at 10,812,077 bp
  • C to A, chromosome 18 at 36,760,384 bp
  • T to A, chromosome 18 at 54,897,779 bp
  • G to T, chromosome 18 at 76,980,011 bp
  • T to C, chromosome 19 at 39,712,528 bp
  • A to G, chromosome 19 at 44,788,832 bp
  • A to G, chromosome X at 23,261,581 bp
  • T to A, chromosome X at 83,830,517 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1952 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039966-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.