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Strain Name:
C57BL/6J-MtgxR2033Btlr/Mmmh
Stock Number:
040040-MU
Citation ID:
RRID:MMRRC_040040-MU
Other Names:
R2033 (G1), C57BL/6J-MtgxR2033Btlr
Major Collection:

Strain Information

Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: Steel Factor Receptor, c-KIT, Dominant white spotting, belly-spot, Tr-kit, SOW3, SCO5, SCO1, Gsfsow3, Gsfsco5, Gsfsco1, CD117
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Slc6a6
Name: solute carrier family 6 (neurotransmitter transporter, taurine), member 6
Synonyms: Taut
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21366
Homologene: 2291
Gldc
Name: glycine decarboxylase
Synonyms: D19Wsu57e, D030049L12Rik, b2b2679Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 104174
VEGA: 19
HGNC: HGNC:4313
Homologene: 141
Ppp1r14c
Name: protein phosphatase 1, regulatory inhibitor subunit 14C
Synonyms: 6330514J04Rik, KEPI
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 76142
VEGA: 10
Homologene: 12805
Ptprk
Name: protein tyrosine phosphatase receptor type K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 52,790,173 bp
  • A to G, chromosome 1 at 53,761,076 bp
  • A to G, chromosome 1 at 67,160,972 bp
  • T to G, chromosome 1 at 82,718,011 bp
  • CACAGA to CA, chromosome 1 at 85,577,154 bp
  • T to C, chromosome 1 at 156,323,738 bp
  • T to A, chromosome 2 at 73,348,182 bp
  • A to G, chromosome 2 at 89,157,154 bp
  • A to T, chromosome 2 at 91,123,032 bp
  • T to C, chromosome 2 at 120,806,849 bp
  • C to T, chromosome 2 at 121,337,627 bp
  • G to A, chromosome 2 at 155,970,892 bp
  • A to T, chromosome 2 at 181,351,863 bp
  • T to C, chromosome 3 at 146,944,234 bp
  • G to T, chromosome 4 at 102,605,295 bp
  • A to G, chromosome 4 at 130,537,246 bp
  • T to C, chromosome 4 at 144,456,383 bp
  • C to T, chromosome 5 at 25,247,538 bp
  • G to C, chromosome 5 at 75,637,317 bp
  • A to G, chromosome 5 at 87,892,419 bp
  • G to T, chromosome 5 at 125,749,289 bp
  • C to T, chromosome 5 at 134,710,106 bp
  • C to T, chromosome 5 at 139,225,438 bp
  • C to T, chromosome 6 at 55,077,723 bp
  • A to T, chromosome 6 at 91,724,910 bp
  • T to C, chromosome 6 at 101,150,954 bp
  • T to A, chromosome 7 at 5,544,820 bp
  • A to C, chromosome 7 at 78,916,533 bp
  • A to G, chromosome 7 at 102,699,162 bp
  • T to C, chromosome 7 at 102,928,408 bp
  • T to C, chromosome 7 at 105,765,548 bp
  • T to C, chromosome 7 at 107,756,907 bp
  • G to T, chromosome 8 at 70,888,587 bp
  • A to G, chromosome 8 at 86,708,942 bp
  • T to A, chromosome 8 at 86,725,270 bp
  • A to G, chromosome 9 at 24,313,352 bp
  • G to T, chromosome 9 at 38,403,373 bp
  • A to G, chromosome 9 at 66,717,558 bp
  • T to C, chromosome 9 at 95,706,843 bp
  • A to G, chromosome 9 at 111,055,870 bp
  • C to T, chromosome 10 at 3,366,979 bp
  • T to C, chromosome 10 at 23,971,094 bp
  • C to A, chromosome 10 at 28,592,767 bp
  • A to G, chromosome 10 at 79,491,819 bp
  • G to A, chromosome 10 at 86,000,632 bp
  • T to C, chromosome 11 at 3,517,781 bp
  • T to C, chromosome 11 at 70,612,508 bp
  • T to C, chromosome 11 at 73,787,438 bp
  • T to A, chromosome 11 at 77,843,099 bp
  • C to A, chromosome 11 at 115,794,328 bp
  • T to C, chromosome 12 at 81,967,160 bp
  • T to C, chromosome 12 at 98,791,386 bp
  • A to T, chromosome 13 at 34,075,456 bp
  • C to A, chromosome 13 at 76,002,872 bp
  • A to C, chromosome 14 at 54,963,645 bp
  • G to T, chromosome 14 at 70,579,616 bp
  • T to C, chromosome 15 at 38,673,966 bp
  • A to G, chromosome 15 at 73,753,769 bp
  • A to G, chromosome 15 at 78,671,896 bp
  • T to A, chromosome 16 at 15,687,352 bp
  • C to T, chromosome 16 at 32,144,157 bp
  • A to G, chromosome 19 at 30,133,470 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2033 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040040-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.