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Strain Name:
C57BL/6J-MtgxR2051Btlr/Mmmh
Stock Number:
040058-MU
Citation ID:
RRID:MMRRC_040058-MU
Other Names:
R2051 (G1), C57BL/6J-MtgxR2051Btlr
Major Collection:

Strain Information

Gjd2
Name: gap junction protein, delta 2
Synonyms: connexin36, Cx36, Gja9
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14617
Homologene: 7734
Chrnb3
Name: cholinergic receptor, nicotinic, beta polypeptide 3
Synonyms: Acrb3, 5730417K16Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 108043
HGNC: HGNC:1963
Homologene: 36035
Prok2
Name: prokineticin 2
Synonyms: Bombina variegata 8kDa protein, Bv8, PK2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 50501
Homologene: 9268
Pkp4
Name: plakophilin 4
Synonyms: p0071, Armrp, 5031422I09Rik, 9430019K17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227937
HGNC: HGNC:9026
Homologene: 2689
Arid4b
Name: AT-rich interaction domain 4B
Synonyms: RBBP1L1, SAP180, BRCAA1, Rbp1l1, 6720480E17Rik, 6330417L24Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 94246
Homologene: 12847
Pds5b
Name: PDS5 cohesin associated factor B
Synonyms: AS3, Aprin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100710
Homologene: 41001
Arhgef10
Name: Rho guanine nucleotide exchange factor 10
Synonyms: 6430549H08Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234094
Homologene: 22827
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 40,726,437 bp
  • T to C, chromosome 1 at 151,372,330 bp
  • T to C, chromosome 1 at 159,892,033 bp
  • T to A, chromosome 1 at 174,089,219 bp
  • G to T, chromosome 2 at 23,132,824 bp
  • A to G, chromosome 2 at 24,436,508 bp
  • T to C, chromosome 2 at 59,334,904 bp
  • G to T, chromosome 2 at 68,711,048 bp
  • C to T, chromosome 2 at 88,391,538 bp
  • C to T, chromosome 2 at 90,757,532 bp
  • T to A, chromosome 2 at 112,756,641 bp
  • T to C, chromosome 2 at 114,011,058 bp
  • A to G, chromosome 2 at 125,109,660 bp
  • T to G, chromosome 2 at 129,015,618 bp
  • G to C, chromosome 2 at 131,240,450 bp
  • C to T, chromosome 2 at 137,066,205 bp
  • A to G, chromosome 2 at 151,503,082 bp
  • A to T, chromosome 2 at 158,632,293 bp
  • A to G, chromosome 3 at 38,454,493 bp
  • T to C, chromosome 3 at 64,117,345 bp
  • T to C, chromosome 3 at 88,120,167 bp
  • T to C, chromosome 3 at 95,240,740 bp
  • G to T, chromosome 3 at 97,168,017 bp
  • T to C, chromosome 3 at 102,777,541 bp
  • T to C, chromosome 3 at 103,809,040 bp
  • G to A, chromosome 3 at 121,981,860 bp
  • T to A, chromosome 4 at 96,769,771 bp
  • T to C, chromosome 4 at 98,780,102 bp
  • C to T, chromosome 4 at 100,407,868 bp
  • T to C, chromosome 4 at 108,192,047 bp
  • T to C, chromosome 4 at 126,460,453 bp
  • T to C, chromosome 4 at 131,819,087 bp
  • T to A, chromosome 5 at 3,975,685 bp
  • G to T, chromosome 5 at 24,591,276 bp
  • T to A, chromosome 5 at 34,462,150 bp
  • T to A, chromosome 5 at 65,798,174 bp
  • A to G, chromosome 5 at 103,525,922 bp
  • A to T, chromosome 5 at 114,245,890 bp
  • A to G, chromosome 5 at 118,046,329 bp
  • G to C, chromosome 5 at 120,543,388 bp
  • G to T, chromosome 5 at 135,970,264 bp
  • T to A, chromosome 5 at 136,332,658 bp
  • G to A, chromosome 5 at 138,564,185 bp
  • T to G, chromosome 5 at 150,748,190 bp
  • A to T, chromosome 5 at 151,562,551 bp
  • T to A, chromosome 6 at 35,230,516 bp
  • A to T, chromosome 6 at 40,879,046 bp
  • C to A, chromosome 6 at 57,625,976 bp
  • T to A, chromosome 6 at 99,714,487 bp
  • T to G, chromosome 7 at 3,665,401 bp
  • T to C, chromosome 7 at 6,712,721 bp
  • T to C, chromosome 7 at 9,217,793 bp
  • T to C, chromosome 7 at 16,141,015 bp
  • C to T, chromosome 7 at 27,476,269 bp
  • C to T, chromosome 7 at 28,120,360 bp
  • C to T, chromosome 7 at 73,943,202 bp
  • T to C, chromosome 7 at 139,096,814 bp
  • A to G, chromosome 8 at 14,945,320 bp
  • T to G, chromosome 8 at 25,691,089 bp
  • C to A, chromosome 8 at 27,386,811 bp
  • T to A, chromosome 8 at 60,507,139 bp
  • T to C, chromosome 8 at 86,049,821 bp
  • C to T, chromosome 8 at 94,942,067 bp
  • A to G, chromosome 8 at 95,724,593 bp
  • A to T, chromosome 8 at 124,672,211 bp
  • A to G, chromosome 8 at 124,895,480 bp
  • C to T, chromosome 9 at 110,550,890 bp
  • A to G, chromosome 10 at 24,028,006 bp
  • T to A, chromosome 10 at 24,091,314 bp
  • A to G, chromosome 10 at 24,711,804 bp
  • T to C, chromosome 10 at 69,898,090 bp
  • A to G, chromosome 10 at 76,531,878 bp
  • T to A, chromosome 10 at 106,837,299 bp
  • T to C, chromosome 10 at 109,824,675 bp
  • A to T, chromosome 11 at 7,161,885 bp
  • A to G, chromosome 11 at 9,328,098 bp
  • A to T, chromosome 11 at 23,464,468 bp
  • C to T, chromosome 11 at 67,939,883 bp
  • G to T, chromosome 11 at 82,440,438 bp
  • T to C, chromosome 11 at 96,541,463 bp
  • T to C, chromosome 11 at 98,420,172 bp
  • A to G, chromosome 11 at 99,949,204 bp
  • C to T, chromosome 11 at 103,063,984 bp
  • A to T, chromosome 11 at 121,453,670 bp
  • T to A, chromosome 12 at 24,586,152 bp
  • C to A, chromosome 12 at 58,990,968 bp
  • A to G, chromosome 13 at 14,187,645 bp
  • A to G, chromosome 13 at 28,952,781 bp
  • C to T, chromosome 13 at 117,976,083 bp
  • A to T, chromosome 14 at 20,142,209 bp
  • T to G, chromosome 14 at 31,279,123 bp
  • A to G, chromosome 14 at 47,411,484 bp
  • A to T, chromosome 15 at 11,393,194 bp
  • C to A, chromosome 15 at 36,162,132 bp
  • G to T, chromosome 15 at 38,933,234 bp
  • C to T, chromosome 15 at 48,621,993 bp
  • A to G, chromosome 15 at 76,348,207 bp
  • C to A, chromosome 15 at 79,004,540 bp
  • A to T, chromosome 15 at 98,131,692 bp
  • T to C, chromosome 15 at 102,029,500 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • C to A, chromosome 16 at 91,580,292 bp
  • T to C, chromosome 17 at 25,366,565 bp
  • A to G, chromosome 17 at 57,741,768 bp
  • G to C, chromosome 18 at 34,738,239 bp
  • G to A, chromosome 18 at 54,988,314 bp
  • A to G, chromosome 19 at 39,082,231 bp
  • A to G, chromosome 19 at 57,691,849 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2051 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040058-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.