Strain Name:
C57BL/6J-MtgxR2064Btlr/Mmmh
Stock Number:
040069-MU
Citation ID:
RRID:MMRRC_040069-MU
Other Names:
R2064 (G1), C57BL/6J-MtgxR2064Btlr
Major Collection:

Gene Information

Gene Symbol: Tyr [MGI:98880] (Mus musculus (mouse))
Name: tyrosinase
Synonyms: skc35, Oca1
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Atg7 [MGI:1921494] (Mus musculus (mouse))
Name: autophagy related 7
Synonyms: 1810013K23Rik, Apg7l
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Ptprz1 [MGI:97816] (Mus musculus (mouse))
Name: protein tyrosine phosphatase, receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, Ptpz, phosphacan, Rptpbeta, Ptprz, RPTPz, PTPbeta, PTPzeta
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Cdkn2d [MGI:105387] (Mus musculus (mouse))
Name: cyclin dependent kinase inhibitor 2D
Synonyms: p19, p19INK4d, INK4d, INK4d
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Rgs7 [MGI:1346089] (Mus musculus (mouse))
Name: regulator of G protein signaling 7
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Ep400 [MGI:1276124] (Mus musculus (mouse))
Name: E1A binding protein p400
Synonyms: 1700020J09Rik, mDomino, p400
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Mapk8 [MGI:1346861] (Mus musculus (mouse))
Name: mitogen-activated protein kinase 8
Synonyms: c-Jun N-terminal kinase, JNK1, Prkm8
Chromosome: 14
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 21,350,972 bp
  • A to G, chromosome 1 at 36,702,087 bp
  • A to G, chromosome 1 at 36,779,134 bp
  • G to A, chromosome 1 at 59,143,782 bp
  • C to A, chromosome 1 at 60,270,356 bp
  • T to C, chromosome 1 at 130,939,117 bp
  • A to T, chromosome 1 at 175,121,942 bp
  • A to G, chromosome 1 at 177,102,985 bp
  • C to T, chromosome 2 at 5,795,543 bp
  • A to G, chromosome 2 at 10,130,574 bp
  • T to C, chromosome 2 at 19,553,759 bp
  • A to T, chromosome 2 at 34,948,190 bp
  • A to G, chromosome 2 at 39,004,741 bp
  • A to T, chromosome 2 at 71,410,160 bp
  • C to A, chromosome 2 at 76,938,331 bp
  • C to A, chromosome 2 at 92,327,077 bp
  • C to A, chromosome 2 at 94,366,547 bp
  • A to G, chromosome 2 at 181,253,970 bp
  • A to G, chromosome 3 at 5,398,927 bp
  • A to T, chromosome 3 at 28,806,808 bp
  • A to C, chromosome 3 at 32,198,288 bp
  • A to G, chromosome 3 at 65,364,639 bp
  • A to G, chromosome 3 at 75,151,403 bp
  • T to C, chromosome 3 at 113,530,568 bp
  • T to C, chromosome 3 at 127,613,350 bp
  • A to G, chromosome 3 at 140,839,217 bp
  • A to T, chromosome 3 at 141,870,807 bp
  • T to C, chromosome 3 at 149,185,159 bp
  • C to T, chromosome 4 at 41,199,872 bp
  • T to A, chromosome 4 at 48,396,287 bp
  • T to A, chromosome 4 at 88,522,759 bp
  • C to T, chromosome 4 at 90,225,120 bp
  • T to C, chromosome 4 at 96,769,726 bp
  • T to C, chromosome 4 at 111,615,126 bp
  • T to A, chromosome 4 at 112,025,533 bp
  • T to A, chromosome 4 at 115,524,720 bp
  • A to T, chromosome 4 at 121,324,872 bp
  • T to A, chromosome 4 at 122,689,322 bp
  • T to C, chromosome 4 at 126,175,396 bp
  • T to C, chromosome 4 at 128,747,136 bp
  • T to A, chromosome 4 at 134,523,307 bp
  • T to A, chromosome 5 at 30,173,798 bp
  • C to T, chromosome 5 at 34,825,982 bp
  • G to A, chromosome 5 at 36,522,930 bp
  • A to C, chromosome 5 at 110,735,404 bp
  • T to C, chromosome 5 at 120,885,883 bp
  • C to T, chromosome 5 at 134,216,498 bp
  • A to T, chromosome 5 at 135,132,777 bp
  • C to T, chromosome 5 at 136,030,577 bp
  • T to C, chromosome 5 at 147,875,453 bp
  • A to G, chromosome 6 at 23,050,389 bp
  • G to T, chromosome 6 at 42,366,053 bp
  • A to T, chromosome 6 at 48,473,662 bp
  • T to G, chromosome 6 at 83,058,543 bp
  • C to T, chromosome 6 at 85,478,471 bp
  • A to G, chromosome 6 at 113,704,142 bp
  • C to A, chromosome 6 at 114,703,363 bp
  • T to C, chromosome 7 at 4,523,799 bp
  • A to G, chromosome 7 at 5,147,049 bp
  • T to G, chromosome 7 at 17,093,748 bp
  • T to A, chromosome 7 at 18,521,253 bp
  • C to T, chromosome 7 at 45,147,161 bp
  • T to C, chromosome 7 at 87,492,843 bp
  • G to A, chromosome 7 at 109,898,693 bp
  • C to T, chromosome 7 at 118,156,867 bp
  • A to T, chromosome 8 at 84,673,118 bp
  • G to A, chromosome 8 at 87,781,358 bp
  • T to C, chromosome 8 at 127,610,611 bp
  • A to G, chromosome 9 at 21,290,879 bp
  • A to G, chromosome 9 at 56,896,656 bp
  • A to G, chromosome 9 at 79,662,454 bp
  • T to C, chromosome 9 at 118,807,293 bp
  • T to C, chromosome 9 at 120,016,896 bp
  • G to T, chromosome 10 at 61,205,522 bp
  • T to C, chromosome 10 at 62,286,536 bp
  • T to A, chromosome 10 at 76,039,183 bp
  • T to C, chromosome 10 at 80,170,332 bp
  • G to A, chromosome 10 at 94,045,041 bp
  • T to C, chromosome 11 at 6,439,365 bp
  • T to C, chromosome 11 at 57,317,708 bp
  • C to T, chromosome 11 at 66,145,435 bp
  • T to C, chromosome 11 at 76,737,398 bp
  • T to A, chromosome 11 at 97,493,062 bp
  • T to A, chromosome 11 at 102,000,690 bp
  • T to C, chromosome 11 at 106,871,548 bp
  • T to C, chromosome 11 at 119,499,582 bp
  • T to C, chromosome 12 at 64,943,472 bp
  • A to T, chromosome 13 at 11,730,333 bp
  • A to G, chromosome 13 at 22,884,115 bp
  • G to A, chromosome 13 at 27,196,737 bp
  • T to A, chromosome 13 at 27,660,887 bp
  • T to C, chromosome 13 at 30,935,561 bp
  • C to T, chromosome 13 at 73,607,723 bp
  • G to A, chromosome 14 at 14,733,773 bp
  • A to G, chromosome 14 at 33,410,846 bp
  • T to C, chromosome 14 at 37,095,105 bp
  • A to G, chromosome 14 at 42,413,701 bp
  • G to A, chromosome 14 at 51,996,183 bp
  • A to G, chromosome 14 at 55,709,471 bp
  • T to C, chromosome 14 at 66,356,260 bp
  • A to G, chromosome 14 at 75,198,075 bp
  • T to A, chromosome 15 at 8,186,165 bp
  • A to G, chromosome 15 at 88,812,115 bp
  • T to C, chromosome 16 at 15,634,469 bp
  • T to C, chromosome 16 at 18,151,257 bp
  • G to A, chromosome 16 at 49,155,621 bp
  • G to A, chromosome 16 at 56,243,630 bp
  • T to A, chromosome 16 at 58,674,409 bp
  • A to T, chromosome 17 at 22,455,465 bp
  • G to A, chromosome 17 at 27,098,076 bp
  • G to A, chromosome 17 at 28,821,364 bp
  • T to A, chromosome 17 at 37,009,676 bp
  • T to A, chromosome 17 at 37,835,002 bp
  • T to A, chromosome 17 at 57,524,553 bp
  • T to C, chromosome 17 at 71,111,800 bp
  • C to T, chromosome 18 at 58,048,849 bp
  • T to C, chromosome 18 at 60,538,277 bp
  • A to T, chromosome 18 at 61,739,122 bp
  • G to T, chromosome 18 at 61,758,743 bp
  • T to C, chromosome 19 at 6,298,078 bp
  • T to C, chromosome 19 at 10,796,317 bp
  • T to A, chromosome X at 67,920,702 bp
  • T to A, chromosome X at 73,771,751 bp
  • A to G, chromosome X at 101,267,810 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2064 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040069-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.