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Strain Name:
C57BL/6J-MtgxR2071Btlr/Mmmh
Stock Number:
040076-MU
Citation ID:
RRID:MMRRC_040076-MU
Other Names:
R2071 (G1), C57BL/6J-MtgxR2071Btlr
Major Collection:

Strain Information

Mc1r
Name: melanocortin 1 receptor
Synonyms: e, Mshra, extension recessive yellow, Mcr1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 17199
HGNC: HGNC:6929
Homologene: 1789
Trp53
Name: transformation related protein 53
Synonyms: p53, p44
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22059
Homologene: 460
Vcp
Name: valosin containing protein
Synonyms: CDC48, p97, p97/VCP, AAA ATPase p97
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269523
Homologene: 5168
Nap1l1
Name: nucleosome assembly protein 1-like 1
Synonyms: D10Ertd68e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 53605
VEGA: 10
HGNC: HGNC:7637
Homologene: 129218
Ccn1
Name: cellular communication network factor 1
Synonyms: CCN1, Cyr61
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 16007
HGNC: HGNC:2654
Homologene: 1194
Nmral1
Name: NmrA-like family domain containing 1
Synonyms: 1110025F24Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 67824
Homologene: 41388
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 57,383,315 bp
  • T to A, chromosome 1 at 57,940,464 bp
  • C to A, chromosome 1 at 75,255,144 bp
  • T to C, chromosome 1 at 131,977,632 bp
  • T to C, chromosome 1 at 151,636,430 bp
  • T to C, chromosome 1 at 155,290,280 bp
  • T to C, chromosome 2 at 62,542,121 bp
  • A to G, chromosome 2 at 91,646,018 bp
  • T to C, chromosome 2 at 112,479,227 bp
  • T to C, chromosome 2 at 131,055,346 bp
  • T to C, chromosome 2 at 144,389,216 bp
  • A to G, chromosome 3 at 55,207,622 bp
  • T to C, chromosome 3 at 87,923,067 bp
  • C to G, chromosome 3 at 88,966,203 bp
  • C to A, chromosome 3 at 95,747,638 bp
  • G to A, chromosome 3 at 96,193,875 bp
  • T to C, chromosome 3 at 130,595,092 bp
  • C to T, chromosome 3 at 145,648,673 bp
  • T to C, chromosome 4 at 10,879,514 bp
  • A to G, chromosome 4 at 22,488,076 bp
  • A to G, chromosome 4 at 42,995,894 bp
  • T to C, chromosome 5 at 73,007,497 bp
  • A to G, chromosome 5 at 90,523,735 bp
  • T to G, chromosome 5 at 93,636,516 bp
  • T to C, chromosome 5 at 110,841,246 bp
  • T to C, chromosome 5 at 121,632,485 bp
  • A to T, chromosome 5 at 123,511,370 bp
  • G to T, chromosome 5 at 123,794,277 bp
  • T to C, chromosome 5 at 125,433,928 bp
  • A to G, chromosome 5 at 143,176,584 bp
  • T to A, chromosome 6 at 71,976,074 bp
  • C to T, chromosome 6 at 90,072,202 bp
  • A to G, chromosome 6 at 142,369,467 bp
  • A to G, chromosome 8 at 80,057,302 bp
  • T to C, chromosome 8 at 84,921,194 bp
  • G to T, chromosome 8 at 85,085,384 bp
  • A to T, chromosome 8 at 95,108,353 bp
  • T to C, chromosome 8 at 123,408,369 bp
  • T to A, chromosome 9 at 7,349,725 bp
  • T to C, chromosome 9 at 15,341,564 bp
  • T to C, chromosome 9 at 41,979,457 bp
  • C to G, chromosome 9 at 62,919,336 bp
  • T to C, chromosome 9 at 80,283,594 bp
  • A to G, chromosome 9 at 110,727,013 bp
  • C to T, chromosome 10 at 24,922,663 bp
  • T to A, chromosome 10 at 39,824,353 bp
  • A to G, chromosome 10 at 109,737,014 bp
  • C to T, chromosome 10 at 111,492,900 bp
  • A to G, chromosome 11 at 58,876,769 bp
  • A to C, chromosome 11 at 62,117,875 bp
  • A to G, chromosome 11 at 69,589,632 bp
  • A to T, chromosome 11 at 80,098,052 bp
  • T to C, chromosome 11 at 83,421,949 bp
  • A to G, chromosome 12 at 8,499,232 bp
  • C to T, chromosome 12 at 76,052,872 bp
  • A to G, chromosome 12 at 79,287,446 bp
  • T to A, chromosome 12 at 87,517,052 bp
  • A to T, chromosome 12 at 102,562,863 bp
  • C to T, chromosome 12 at 112,920,219 bp
  • T to C, chromosome 13 at 22,474,825 bp
  • T to A, chromosome 13 at 34,128,261 bp
  • G to A, chromosome 13 at 52,835,496 bp
  • A to T, chromosome 13 at 76,759,724 bp
  • T to A, chromosome 13 at 77,104,624 bp
  • A to T, chromosome 13 at 119,912,300 bp
  • A to G, chromosome 14 at 16,241,983 bp
  • T to A, chromosome 14 at 20,303,977 bp
  • T to C, chromosome 14 at 50,854,282 bp
  • A to G, chromosome 14 at 56,366,009 bp
  • A to G, chromosome 14 at 65,235,563 bp
  • T to A, chromosome 14 at 67,728,755 bp
  • T to C, chromosome 14 at 99,062,278 bp
  • A to G, chromosome 14 at 111,680,189 bp
  • T to A, chromosome 16 at 4,716,347 bp
  • T to A, chromosome 16 at 32,372,469 bp
  • C to T, chromosome 16 at 48,592,611 bp
  • A to T, chromosome 17 at 8,961,995 bp
  • A to T, chromosome 17 at 19,793,794 bp
  • T to C, chromosome 17 at 26,122,043 bp
  • A to G, chromosome 17 at 29,694,783 bp
  • A to T, chromosome 17 at 35,554,694 bp
  • G to C, chromosome 17 at 46,303,565 bp
  • T to C, chromosome 17 at 58,974,097 bp
  • T to C, chromosome 17 at 78,841,085 bp
  • G to A, chromosome 17 at 80,216,601 bp
  • T to C, chromosome 18 at 20,536,825 bp
  • T to C, chromosome 19 at 6,257,458 bp
  • T to A, chromosome 19 at 55,222,340 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2071 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040076-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.