Strain Name:
C57BL/6J-MtgxR2095Btlr/Mmmh
Stock Number:
040099-MU
Citation ID:
RRID:MMRRC_040099-MU
Other Names:
R2095 (G1), C57BL/6J-MtgxR2095Btlr
Major Collection:

Gene Information

Chrnb2
Name: cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal)
Synonyms: [b]2-nAchR, C030030P04Rik, Acrb2, Acrb-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 11444
HGNC: HGNC:1962
Homologene: 595
Pzp
Name: PZP, alpha-2-macroglobulin like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 11287
Homologene: 104112
Stub1
Name: STIP1 homology and U-Box containing protein 1
Synonyms: 2310040B03Rik, 0610033N24Rik, 2210017D18Rik, CHIP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 56424
VEGA: 17
Homologene: 4281
Slit1
Name: slit guidance ligand 1
Synonyms: Slil1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20562
Homologene: 2302
Ccn2
Name: cellular communication network factor 2
Synonyms: hypertrophic chondrocyte-specific gene product 24, Ccn2, Ctgf, Hcs24, Fisp12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 14219
HGNC: HGNC:2500
Homologene: 1431
Ubap2
Name: ubiquitin-associated protein 2
Synonyms: 1190005K07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 68926
Homologene: 73649
Phf14
Name: PHD finger protein 14
Synonyms: 4932409F11Rik, 5730446A07Rik, 1110001C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 75725
Homologene: 8775
Mov10
Name: Mov10 RISC complex RNA helicase
Synonyms: Mov-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 17454
HGNC: HGNC:7200
Homologene: 10365
Plekhm1
Name: pleckstrin homology domain containing, family M (with RUN domain) member 1
Synonyms: AP162, D330036J23Rik, B2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 353047
Homologene: 8871
Bbx
Name: bobby sox HMG box containing
Synonyms: 5530401J07Rik, 5730403O13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 70508
Homologene: 10634
Arhgap21
Name: Rho GTPase activating protein 21
Synonyms: 5530401C11Rik, ARHGAP10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71435
Homologene: 10822
Ube3c
Name: ubiquitin protein ligase E3C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100763
Homologene: 8783
Uaca
Name: uveal autoantigen with coiled-coil domains and ankyrin repeats
Synonyms: 2700059D02Rik, nucling
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72565
VEGA: 9
Homologene: 74297
Helz
Name: helicase with zinc finger domain
Synonyms: 3110078M01Rik, 9630002H22Rik, 9430093I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 78455
Homologene: 8918
Fnta
Name: farnesyltransferase, CAAX box, alpha
Synonyms: FTA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 14272
HGNC: HGNC:3782
Homologene: 1534
Slc33a1
Name: solute carrier family 33 (acetyl-CoA transporter), member 1
Synonyms: Acatn, D630022N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 11416
HGNC: HGNC:95
Homologene: 3476
Cluh
Name: clustered mitochondria (cluA/CLU1) homolog
Synonyms: 1300001I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74148
Homologene: 9063
Dtna
Name: dystrobrevin alpha
Synonyms: Dtn, 2210407P21Rik, a-DB-1, 87K protein, A0, alpha-dystrobrevin, adbn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13527
VEGA: 18
HGNC: HGNC:3057
Homologene: 20362
Fntb
Name: farnesyltransferase, CAAX box, beta
Synonyms: 2010013E13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 110606
HGNC: HGNC:3785
Homologene: 1535
Frs2
Name: fibroblast growth factor receptor substrate 2
Synonyms: SNT1, C330018A15Rik, Frs2alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 327826
VEGA: 10
Homologene: 4846
Klf3
Name: Kruppel-like factor 3 (basic)
Synonyms: Bklf, 9930027G08Rik, Tef-2, BKLF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 16599
Homologene: 7396
Abr
Name: active BCR-related gene
Synonyms: 6330400K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 109934
HGNC: HGNC:81
Homologene: 11081
Pgm3
Name: phosphoglucomutase 3
Synonyms: Pgm-3, 2810473H05Rik, GlcNAc-P mutase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 109785
HGNC: HGNC:8907
Homologene: 9205
Msh6
Name: mutS homolog 6
Synonyms: Gtmbp, GTBP, Msh6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 17688
VEGA: 17
HGNC: HGNC:7329
Homologene: 149
Tbc1d1
Name: TBC1 domain family, member 1
Synonyms: Nobq1, 1110062G02Rik, Nob1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 57915
Homologene: 56856
Arhgef17
Name: Rho guanine nucleotide exchange factor (GEF) 17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 207212
Homologene: 129601
Zfp738
Name: zinc finger protein 738
Synonyms: 6720487G11Rik, 3830402I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 408068
Homologene: 133713
Adam33
Name: a disintegrin and metallopeptidase domain 33
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 110751
Homologene: 11881
Svil
Name: supervillin
Synonyms: B430302E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225115
Homologene: 25090
Gnl2
Name: guanine nucleotide binding protein-like 2 (nucleolar)
Synonyms: Ngp-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230737
Homologene: 6858
Clec9a
Name: C-type lectin domain family 9, member a
Synonyms: DNGR-1, 9830005G06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232414
Homologene: 52106
Cacna2d2
Name: calcium channel, voltage-dependent, alpha 2/delta subunit 2
Synonyms: a2d2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 56808
HGNC: HGNC:1400
Homologene: 4400
Hydin
Name: HYDIN, axonemal central pair apparatus protein
Synonyms: hy-3, 4930545D19Rik, 1700034M11Rik, hyrh, hy3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244653
Homologene: 52118
Spta1
Name: spectrin alpha, erythrocytic 1
Synonyms: Spna1, erythroid, Spna-1, ihj
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20739
Homologene: 74460
Mlxipl
Name: MLX interacting protein-like
Synonyms: ChREBP, Wbscr14, bHLHd14, WS-bHLH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 58805
Homologene: 32507
Tcea2
Name: transcription elongation factor A (SII), 2
Synonyms: SII-T1, Tceat, S-II-T1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21400
Homologene: 68304
Ctdspl
Name: CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like
Synonyms: HYA22, 2810418J22Rik, SCP3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 69274
Homologene: 48881
Myh8
Name: myosin, heavy polypeptide 8, skeletal muscle, perinatal
Synonyms: MyHC-pn, Myhs-p, Myhsp, 4832426G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17885
HGNC: HGNC:7578
Homologene: 68256
Armc3
Name: armadillo repeat containing 3
Synonyms: 4921513G22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 70882
Homologene: 31589
Casq1
Name: calsequestrin 1
Synonyms: CSQ, sCSQ, CSQ-1, CSQ1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12372
HGNC: HGNC:1512
Homologene: 20329
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: OTTMUSG00000005786, LOC380698
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Dcaf12
Name: DDB1 and CUL4 associated factor 12
Synonyms: 1500001L20Rik, Wdr40a, 5830424K06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 68970
Homologene: 49418
Sgcz
Name: sarcoglycan zeta
Synonyms: C230085N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244431
Homologene: 26726
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 435626
Homologene: 52359
Cyp3a57
Name: cytochrome P450, family 3, subfamily a, polypeptide 57
Synonyms: EG622127
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 622127
Homologene: 135775
Cbfa2t3
Name: CBFA2/RUNX1 translocation partner 3
Synonyms: MTGR2, Eto2, A630044F12Rik, ETO-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12398
HGNC: HGNC:1537
Homologene: 74543
Olfr381
Name: olfactory receptor 381
Synonyms: GA_x6K02T2P1NL-3646409-3645474, MOR135-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 259024
Kctd11
Name: potassium channel tetramerisation domain containing 11
Synonyms: Ren
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216858
Homologene: 17697
Cntnap5a
Name: contactin associated protein-like 5A
Synonyms: Caspr5-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 636808
Homologene: 43974
9130401M01Rik
Name: RIKEN cDNA 9130401M01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 75758
Homologene: 12622
Vmn2r8
Name: vomeronasal 2, receptor 8
Synonyms: EG627479
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 627479
Homologene: 129606
Pcdhb17
Name: protocadherin beta 17
Synonyms: Pcdhb16, PcdhbQ
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93888
Homologene: 81881
Tcp11l1
Name: t-complex 11 like 1
Synonyms: C130096D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320554
Homologene: 41260
9530002B09Rik
Name: RIKEN cDNA 9530002B09 gene
Synonyms: Aump, Vpp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 77432
S1pr5
Name: sphingosine-1-phosphate receptor 5
Synonyms: lpB4, Edg8, S1P5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 94226
Homologene: 11031
Arl5c
Name: ADP-ribosylation factor-like 5C
Synonyms: Arl12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217151
Homologene: 28456
Slco2a1
Name: solute carrier organic anion transporter family, member 2a1
Synonyms: 2310021C19Rik, mPgt, Slc21a2, Pgt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 24059
Homologene: 38077
Adh1
Name: alcohol dehydrogenase 1 (class I)
Synonyms: Adh-1e, Adh-1-t, Adh1-t, Adh-1, Adh1tl, Adh-1t, class I alcohol dehydrogenase, ADH-AA, Adh1-e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 11522
Homologene: 73888
Klhl21
Name: kelch-like 21
Synonyms: 1810045K06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242785
Homologene: 8902
Cd163
Name: CD163 antigen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 93671
HGNC: HGNC:1631
Homologene: 128811
Olfr166
Name: olfactory receptor 166
Synonyms: MOR270-1, GA_x54KRFPKG5P-15934912-15935850
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 259071
Homologene: 88350
Olfr1205
Name: olfactory receptor 1205
Synonyms: Olfr1203, MOR230-3, GA_x6K02T2Q125-50336588-50337313, GA_x6K02T2Q125-50304328-50305251, MOR230-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258898
Homologene: 81567
Trpv6
Name: transient receptor potential cation channel, subfamily V, member 6
Synonyms: Ecac2, Cac, CaT1, CAT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 64177
Homologene: 56812
Lsm14b
Name: LSM family member 14B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241846
Homologene: 100207
Olfr150
Name: olfactory receptor 150
Synonyms: MOR171-18, GA_x6K02T2PVTD-33434302-33435240, M93
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258602
VEGA: 9
Gabra4
Name: gamma-aminobutyric acid (GABA) A receptor, subunit alpha 4
Synonyms: Gabra-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14397
HGNC: HGNC:4078
Homologene: 631
Gm2431
Name: predicted gene 2431
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 105243101
Slc25a31
Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 31
Synonyms: 1700034J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 73333
Homologene: 69485
Ndufv3
Name: NADH:ubiquinone oxidoreductase core subunit V3
Synonyms: 1500032D16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 78330
HGNC: HGNC:7719
Homologene: 10885
Taar7e
Name: trace amine-associated receptor 7E
Synonyms: LOC276742
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 276742
Homologene: 134040
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 74,147,663 bp
  • T to A, chromosome 1 at 116,442,260 bp
  • T to C, chromosome 1 at 172,215,962 bp
  • T to A, chromosome 1 at 174,244,198 bp
  • T to A, chromosome 2 at 19,288,929 bp
  • T to C, chromosome 2 at 20,967,318 bp
  • T to C, chromosome 2 at 88,831,290 bp
  • T to A, chromosome 2 at 104,681,840 bp
  • C to T, chromosome 2 at 131,053,709 bp
  • A to T, chromosome 2 at 180,031,787 bp
  • C to A, chromosome 2 at 181,686,932 bp
  • T to A, chromosome 3 at 40,722,762 bp
  • A to G, chromosome 3 at 63,963,955 bp
  • A to T, chromosome 3 at 89,761,437 bp
  • G to A, chromosome 3 at 104,801,531 bp
  • T to C, chromosome 3 at 138,282,796 bp
  • C to G, chromosome 4 at 41,206,901 bp
  • G to T, chromosome 4 at 41,294,085 bp
  • C to A, chromosome 4 at 122,702,358 bp
  • C to T, chromosome 4 at 125,034,318 bp
  • T to C, chromosome 4 at 152,009,393 bp
  • T to C, chromosome 5 at 29,668,040 bp
  • C to T, chromosome 5 at 64,316,501 bp
  • T to A, chromosome 5 at 64,821,902 bp
  • T to C, chromosome 5 at 71,624,112 bp
  • T to G, chromosome 5 at 108,808,621 bp
  • T to C, chromosome 5 at 135,122,120 bp
  • A to T, chromosome 5 at 145,369,134 bp
  • T to A, chromosome 6 at 11,990,146 bp
  • C to T, chromosome 6 at 41,621,756 bp
  • A to G, chromosome 6 at 124,317,822 bp
  • C to T, chromosome 6 at 128,516,801 bp
  • T to C, chromosome 6 at 129,416,358 bp
  • T to C, chromosome 7 at 100,881,263 bp
  • A to T, chromosome 7 at 142,257,781 bp
  • G to A, chromosome 8 at 25,999,879 bp
  • T to C, chromosome 8 at 37,540,392 bp
  • A to G, chromosome 8 at 110,462,657 bp
  • A to G, chromosome 8 at 122,634,988 bp
  • T to A, chromosome 9 at 21,244,858 bp
  • T to A, chromosome 9 at 39,737,261 bp
  • T to A, chromosome 9 at 60,840,843 bp
  • T to C, chromosome 9 at 86,556,341 bp
  • A to G, chromosome 9 at 103,076,968 bp
  • G to A, chromosome 9 at 107,527,165 bp
  • A to G, chromosome 9 at 119,037,346 bp
  • T to A, chromosome 10 at 24,038,051 bp
  • T to C, chromosome 10 at 24,596,479 bp
  • T to C, chromosome 10 at 117,074,602 bp
  • A to T, chromosome 11 at 59,093,584 bp
  • G to T, chromosome 11 at 67,286,224 bp
  • T to A, chromosome 11 at 69,879,576 bp
  • T to A, chromosome 11 at 73,486,594 bp
  • C to T, chromosome 11 at 74,661,724 bp
  • A to G, chromosome 11 at 76,455,594 bp
  • C to T, chromosome 11 at 97,993,451 bp
  • T to A, chromosome 11 at 103,397,283 bp
  • T to A, chromosome 11 at 107,646,146 bp
  • C to A, chromosome 12 at 76,887,888 bp
  • G to T, chromosome 13 at 67,671,303 bp
  • A to T, chromosome 15 at 58,032,092 bp
  • T to C, chromosome 16 at 19,486,931 bp
  • T to C, chromosome 16 at 50,224,689 bp
  • A to G, chromosome 17 at 25,830,890 bp
  • T to C, chromosome 17 at 31,527,486 bp
  • A to G, chromosome 17 at 87,988,233 bp
  • T to C, chromosome 18 at 5,093,009 bp
  • T to A, chromosome 18 at 23,569,748 bp
  • T to A, chromosome 18 at 37,486,322 bp
  • G to A, chromosome 19 at 41,606,380 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2095 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040099-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.