Strain Name:
C57BL/6J-MtgxR2101Btlr/Mmmh
Stock Number:
040105-MU
Citation ID:
RRID:MMRRC_040105-MU
Other Names:
R2101 (G1), C57BL/6J-MtgxR2101Btlr
Major Collection:

Gene Information

Myo10
Name: myosin X
Synonyms: myosin-X, D15Ertd600e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 17909
HGNC: HGNC:7593
Homologene: 36328
Gdf6
Name: growth differentiation factor 6
Synonyms: BMP13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242316
HGNC: HGNC:4221
Homologene: 40883
Actn4
Name: actinin alpha 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 60595
HGNC: HGNC:166
Homologene: 55857
Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Prkd2
Name: protein kinase D2
Synonyms: PKD2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101540
Homologene: 9516
Pcbp3
Name: poly(rC) binding protein 3
Synonyms: AlphaCP-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 59093
HGNC: HGNC:8651
Homologene: 23233
Chd9
Name: chromodomain helicase DNA binding protein 9
Synonyms: 1810014J18Rik, AD013, A330063D19Rik, 9030205D12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 109151
Homologene: 11844
Hunk
Name: hormonally upregulated Neu-associated kinase
Synonyms: Mak-v, Bstk1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 26559
VEGA: 16
Homologene: 8742
Sf3a3
Name: splicing factor 3a, subunit 3
Synonyms: 4930512K19Rik, 60kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 75062
Homologene: 4949
Prc1
Name: protein regulator of cytokinesis 1
Synonyms: D7Ertd348e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233406
HGNC: HGNC:9341
Homologene: 37868
Apaf1
Name: apoptotic peptidase activating factor 1
Synonyms: Apaf1l, 6230400I06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 11783
HGNC: HGNC:576
Homologene: 7626
Cacnb1
Name: calcium channel, voltage-dependent, beta 1 subunit
Synonyms: Cchlb1, Cchb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12295
HGNC: HGNC:1401
Homologene: 20186
Slc38a10
Name: solute carrier family 38, member 10
Synonyms: 1810073N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72055
Homologene: 41556
Cyfip2
Name: cytoplasmic FMR1 interacting protein 2
Synonyms: Pir121, 6430511D02Rik, 1500004I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76884
Homologene: 7936
Cluh
Name: clustered mitochondria (cluA/CLU1) homolog
Synonyms: 1300001I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74148
Homologene: 9063
Txnrd1
Name: thioredoxin reductase 1
Synonyms: TrxR1, TR1, TR alpha, TR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 50493
VEGA: 10
Homologene: 55733
Nuf2
Name: NUF2, NDC80 kinetochore complex component
Synonyms: 2410003C07Rik, Nuf2R, Cdca1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66977
Homologene: 40205
N4bp2
Name: NEDD4 binding protein 2
Synonyms: LOC333789, LOC386488, B3bp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 333789
Homologene: 32396
Gtf2b
Name: general transcription factor IIB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229906
HGNC: HGNC:4648
Homologene: 1158
Clca2
Name: chloride channel accessory 2
Synonyms: Clca5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229933
HGNC: HGNC:2016
Homologene: 4765
Dcc
Name: deleted in colorectal carcinoma
Synonyms: C030036D22Rik, Igdcc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13176
HGNC: HGNC:2701
Homologene: 21081
Uty
Name: ubiquitously transcribed tetratricopeptide repeat containing, Y-linked
Synonyms: Hydb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: Y
NCBI: 22290
Agap3
Name: ArfGAP with GTPase domain, ankyrin repeat and PH domain 3
Synonyms: MRIP-1, Crag, Centg3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 213990
Homologene: 23742
Tspan12
Name: tetraspanin 12
Synonyms: Tm4sf12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 269831
Homologene: 8212
Rbp3
Name: retinol binding protein 3, interstitial
Synonyms: Irbp, Rbp-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 19661
VEGA: 14
HGNC: HGNC:9921
Homologene: 9261
Tfeb
Name: transcription factor EB
Synonyms: TFEB, bHLHe35, Tcfeb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 21425
Homologene: 5182
Hps3
Name: HPS3, biogenesis of lysosomal organelles complex 2 subunit 1
Synonyms: Hermansky-Pudlak syndrome 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12807
Homologene: 13019
Cdc42bpa
Name: CDC42 binding protein kinase alpha
Synonyms: DMPK-like, A930014J19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226751
HGNC: HGNC:1737
Homologene: 55765
Enpep
Name: glutamyl aminopeptidase
Synonyms: Bp-1/6C3, APA, Ly-51, Ly51, 6030431M22Rik, aminopeptidase-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 13809
HGNC: HGNC:3355
Homologene: 68216
Kidins220
Name: kinase D-interacting substrate 220
Synonyms: 3110039L19Rik, C330002I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 77480
VEGA: 12
Homologene: 14254
Rnpep
Name: arginyl aminopeptidase (aminopeptidase B)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 215615
Homologene: 10628
Nlrp1b
Name: NLR family, pyrin domain containing 1B
Synonyms: Nalp1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 637515
Homologene: 19080
Col11a2
Name: collagen, type XI, alpha 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12815
HGNC: HGNC:2187
Homologene: 22547
Chd3
Name: chromodomain helicase DNA binding protein 3
Synonyms: Mi-2 alpha, Prp9-1, Prp7, 2600010P09Rik, Chd7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216848
HGNC: HGNC:1918
Homologene: 62693
Plb1
Name: phospholipase B1
Synonyms: 4632413E21Rik, 4930433E17Rik, 4930539A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 665270
Homologene: 82108
Plekhg4
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 4
Synonyms: 4931414L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102075
Homologene: 18516
Astn2
Name: astrotactin 2
Synonyms: 1d8, Astnl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56079
Homologene: 77850
Rb1cc1
Name: RB1-inducible coiled-coil 1
Synonyms: LaXp180, 2900055E04Rik, Cc1, 5930404L04Rik, Fip200
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12421
Homologene: 7659
Dscam
Name: DS cell adhesion molecule
Synonyms: 4932410A21Rik, Down syndrome cell adhesion molecule
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 13508
HGNC: HGNC:3039
Homologene: 74393
D430041D05Rik
Name: RIKEN cDNA D430041D05 gene
Synonyms: G2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241589
Homologene: 115928
Sarm1
Name: sterile alpha and HEAT/Armadillo motif containing 1
Synonyms: MyD88-5, A830091I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237868
Homologene: 9015
Zc3h12c
Name: zinc finger CCCH type containing 12C
Synonyms: C230027N18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244871
VEGA: 9
Homologene: 35462
Tdrd5
Name: tudor domain containing 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 214575
Homologene: 18312
Olfr1223
Name: olfactory receptor 1223
Synonyms: GA_x6K02T2Q125-50623664-50622729, MOR233-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258894
Homologene: 79379
Dpy19l1
Name: dpy-19-like 1 (C. elegans)
Synonyms: 1100001I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244745
Homologene: 14762
Rev3l
Name: REV3 like, DNA directed polymerase zeta catalytic subunit
Synonyms: Sez4, Rev
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19714
HGNC: HGNC:9968
Homologene: 48147
Slco6c1
Name: solute carrier organic anion transporter family, member 6c1
Synonyms: 4933404A18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74441
Homologene: 65259
Ddx60
Name: DExD/H box helicase 60
Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234311
Homologene: 23031
Nol4
Name: nucleolar protein 4
Synonyms: 4930568N03Rik, LOC383304, 1700013J13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 319211
HGNC: HGNC:7870
Homologene: 36142
Zfp944
Name: zinc finger protein 944
Synonyms: 6330416L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 319615
VEGA: 17
Homologene: 133237
Nemp2
Name: nuclear envelope integral membrane protein 2
Synonyms: 5330401P04Rik, Tmem194b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227094
Homologene: 67089
Dio2
Name: deiodinase, iodothyronine, type II
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13371
VEGA: 12
HGNC: HGNC:2884
Homologene: 621
Abtb2
Name: ankyrin repeat and BTB (POZ) domain containing 2
Synonyms: BPOZ-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99382
Homologene: 15904
Vstm2a
Name: V-set and transmembrane domain containing 2A
Synonyms: 9330184N17Rik, Vstm2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 211739
Homologene: 17123
Vmn2r99
Name: vomeronasal 2, receptor 99
Synonyms: EG665376
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 665376
Homologene: 115024
Cnga1
Name: cyclic nucleotide gated channel alpha 1
Synonyms: Cncg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12788
HGNC: HGNC:2148
Homologene: 55432
Cdkn2aip
Name: CDKN2A interacting protein
Synonyms: CARF, 4921511I16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 70925
Homologene: 9752
Ptprb
Name: protein tyrosine phosphatase, receptor type, B
Synonyms: 3230402H02Rik, VE-PTP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19263
VEGA: 10
HGNC: HGNC:9665
Homologene: 2125
Hmbox1
Name: homeobox containing 1
Synonyms: F830020C16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219150
Homologene: 11589
Vmn1r23
Name: vomeronasal 1 receptor 23
Synonyms: V1rc24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 171197
Homologene: 110825
Krt1
Name: keratin 1
Synonyms: Krt2-1, Krt-2.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16678
VEGA: 15
HGNC: HGNC:6412
Homologene: 38146
Tas2r136
Name: taste receptor, type 2, member 136
Synonyms: mt2r52, Tas2r36
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 353165
Homologene: 136304
Upk1b
Name: uroplakin 1B
Synonyms: Upk1, Tspan20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 22268
VEGA: 16
Homologene: 5065
Marchf2
Name: membrane associated ring-CH-type finger 2
Synonyms: 9530046H09Rik, March2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224703
Homologene: 9539
Nkain2
Name: Na+/K+ transporting ATPase interacting 2
Synonyms: 6330571D19Rik, Tcba1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 432450
Homologene: 65073
Hsd3b6
Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 6
Synonyms: 3beta-HSD VI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 15497
Homologene: 133013
Nat8l
Name: N-acetyltransferase 8-like
Synonyms: 1110038O08Rik, Shati
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269642
Homologene: 19546
Pla1a
Name: phospholipase A1 member A
Synonyms: Ps-pla1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 85031
VEGA: 16
Homologene: 9294
Pcp4l1
Name: Purkinje cell protein 4-like 1
Synonyms: 1500036F01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66425
Homologene: 136091
Tax1bp3
Name: Tax1 (human T cell leukemia virus type I) binding protein 3
Synonyms: TIP-1, 1300011C24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76281
Homologene: 8749
Gm6169
Name: predicted gene 6169
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 620648
VEGA: 13
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 6,249,335 bp
  • T to C, chromosome 1 at 52,641,066 bp
  • A to T, chromosome 1 at 97,072,870 bp
  • G to T, chromosome 1 at 135,271,617 bp
  • A to G, chromosome 1 at 156,301,639 bp
  • A to G, chromosome 1 at 169,523,988 bp
  • G to T, chromosome 1 at 171,175,605 bp
  • G to T, chromosome 1 at 180,146,968 bp
  • T to A, chromosome 2 at 89,144,957 bp
  • G to A, chromosome 2 at 103,566,862 bp
  • A to G, chromosome 2 at 104,148,830 bp
  • C to A, chromosome 3 at 20,012,783 bp
  • T to A, chromosome 3 at 98,806,237 bp
  • C to T, chromosome 3 at 129,298,938 bp
  • T to C, chromosome 3 at 142,771,383 bp
  • T to C, chromosome 3 at 145,077,938 bp
  • A to G, chromosome 4 at 9,860,025 bp
  • G to A, chromosome 4 at 65,581,686 bp
  • C to T, chromosome 4 at 124,718,343 bp
  • T to A, chromosome 5 at 24,487,799 bp
  • T to A, chromosome 5 at 32,349,660 bp
  • T to C, chromosome 5 at 33,998,372 bp
  • T to A, chromosome 5 at 65,790,881 bp
  • C to T, chromosome 5 at 72,619,061 bp
  • A to T, chromosome 6 at 21,799,888 bp
  • T to C, chromosome 6 at 57,926,452 bp
  • T to C, chromosome 6 at 132,777,532 bp
  • T to A, chromosome 7 at 16,869,565 bp
  • G to A, chromosome 7 at 28,898,266 bp
  • T to C, chromosome 7 at 80,312,284 bp
  • A to T, chromosome 8 at 47,713,001 bp
  • T to A, chromosome 8 at 61,940,645 bp
  • C to T, chromosome 8 at 91,033,987 bp
  • T to C, chromosome 8 at 105,378,291 bp
  • A to T, chromosome 9 at 24,482,035 bp
  • A to G, chromosome 9 at 52,116,421 bp
  • G to A, chromosome 10 at 32,329,817 bp
  • T to C, chromosome 10 at 39,828,096 bp
  • T to C, chromosome 10 at 76,789,755 bp
  • T to G, chromosome 10 at 82,881,739 bp
  • T to G, chromosome 10 at 91,060,080 bp
  • C to T, chromosome 10 at 116,315,038 bp
  • T to A, chromosome 11 at 16,263,191 bp
  • A to G, chromosome 11 at 46,242,443 bp
  • A to T, chromosome 11 at 69,349,051 bp
  • A to T, chromosome 11 at 71,165,558 bp
  • G to A, chromosome 11 at 73,181,121 bp
  • G to T, chromosome 11 at 74,660,502 bp
  • G to A, chromosome 11 at 78,475,289 bp
  • A to C, chromosome 11 at 98,005,728 bp
  • A to T, chromosome 11 at 120,132,741 bp
  • T to A, chromosome 12 at 25,057,423 bp
  • T to C, chromosome 12 at 90,729,823 bp
  • G to T, chromosome 13 at 97,099,222 bp
  • C to T, chromosome 14 at 33,956,018 bp
  • A to G, chromosome 14 at 64,828,579 bp
  • G to T, chromosome 14 at 67,794,010 bp
  • T to A, chromosome 15 at 25,722,259 bp
  • C to T, chromosome 15 at 101,846,187 bp
  • T to C, chromosome 16 at 38,415,368 bp
  • A to T, chromosome 16 at 38,780,137 bp
  • T to A, chromosome 16 at 90,432,500 bp
  • T to C, chromosome 16 at 96,610,349 bp
  • T to G, chromosome 17 at 19,377,991 bp
  • T to A, chromosome 17 at 22,339,828 bp
  • G to T, chromosome 17 at 33,709,986 bp
  • A to T, chromosome 17 at 34,052,169 bp
  • G to A, chromosome 17 at 47,789,665 bp
  • A to G, chromosome 18 at 22,823,409 bp
  • G to A, chromosome 18 at 71,810,870 bp
  • T to C, chromosome Y at 1,176,541 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2101 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040105-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.