Strain Name:
C57BL/6J-MtgxR2104Btlr/Mmmh
Stock Number:
040108-MU
Citation ID:
RRID:MMRRC_040108-MU
Other Names:
R2104 (G1), C57BL/6J-MtgxR2104Btlr
Major Collection:

Gene Information

Gene Symbol: Kifc2 [MGI:109187] (Mus musculus (mouse))
Name: kinesin family member C2
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Traf4 [MGI:1202880] (Mus musculus (mouse))
Name: TNF receptor associated factor 4
Synonyms: msp2, CART1, A530032M13Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Cdk6 [MGI:1277162] (Mus musculus (mouse))
Name: cyclin-dependent kinase 6
Synonyms: Crk2
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Igf2bp1 [MGI:1890357] (Mus musculus (mouse))
Name: insulin-like growth factor 2 mRNA binding protein 1
Synonyms: D11Moh40e, Zbp1, IMP1, D030026A21Rik, IMP-1, CRD-BP, D11Moh45, Crdbp
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Cdh1 [MGI:88354] (Mus musculus (mouse))
Name: cadherin 1
Synonyms: E-cadherin, uvomorulin, UM, Ecad, E-cad, L-CAM
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Slit1 [MGI:1315203] (Mus musculus (mouse))
Name: slit guidance ligand 1
Synonyms: Slil1
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Ctnnal1 [MGI:1859649] (Mus musculus (mouse))
Name: catenin (cadherin associated protein), alpha-like 1
Synonyms: ACRP, Catnal1
Chromosome: 4
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,588,678 bp
  • T to G, chromosome 1 at 52,799,418 bp
  • A to G, chromosome 1 at 135,843,809 bp
  • T to C, chromosome 1 at 179,670,597 bp
  • A to G, chromosome 2 at 11,779,900 bp
  • T to A, chromosome 2 at 52,256,814 bp
  • C to A, chromosome 2 at 52,271,558 bp
  • G to A, chromosome 2 at 62,610,545 bp
  • G to A, chromosome 2 at 130,037,483 bp
  • T to A, chromosome 2 at 160,704,819 bp
  • C to T, chromosome 2 at 163,864,865 bp
  • G to T, chromosome 3 at 57,672,875 bp
  • A to G, chromosome 3 at 87,536,062 bp
  • G to T, chromosome 3 at 101,823,428 bp
  • T to A, chromosome 3 at 129,918,688 bp
  • T to G, chromosome 4 at 24,591,084 bp
  • T to C, chromosome 4 at 32,644,727 bp
  • A to T, chromosome 4 at 32,743,843 bp
  • T to C, chromosome 4 at 56,812,329 bp
  • C to A, chromosome 4 at 61,833,725 bp
  • T to C, chromosome 4 at 115,246,918 bp
  • T to A, chromosome 4 at 151,453,294 bp
  • T to C, chromosome 4 at 155,862,131 bp
  • T to G, chromosome 5 at 3,344,387 bp
  • C to A, chromosome 5 at 44,033,020 bp
  • T to A, chromosome 5 at 69,521,119 bp
  • A to T, chromosome 5 at 73,421,530 bp
  • A to T, chromosome 5 at 87,329,682 bp
  • A to T, chromosome 5 at 88,501,787 bp
  • C to T, chromosome 5 at 105,607,358 bp
  • C to T, chromosome 5 at 110,341,141 bp
  • T to C, chromosome 5 at 111,454,751 bp
  • A to T, chromosome 5 at 114,911,002 bp
  • G to A, chromosome 5 at 123,501,114 bp
  • A to G, chromosome 5 at 130,102,361 bp
  • A to G, chromosome 6 at 23,247,332 bp
  • T to C, chromosome 6 at 29,450,735 bp
  • G to A, chromosome 6 at 83,297,378 bp
  • G to A, chromosome 6 at 125,646,330 bp
  • T to A, chromosome 7 at 59,276,477 bp
  • T to A, chromosome 7 at 79,723,239 bp
  • C to T, chromosome 7 at 81,591,771 bp
  • G to A, chromosome 7 at 119,784,304 bp
  • C to A, chromosome 7 at 141,634,078 bp
  • C to A, chromosome 8 at 69,882,792 bp
  • T to A, chromosome 8 at 106,653,759 bp
  • G to A, chromosome 8 at 119,749,845 bp
  • A to G, chromosome 8 at 121,118,080 bp
  • A to G, chromosome 9 at 15,998,517 bp
  • A to G, chromosome 9 at 54,415,564 bp
  • C to T, chromosome 9 at 65,074,567 bp
  • T to C, chromosome 9 at 103,317,982 bp
  • C to A, chromosome 9 at 107,996,261 bp
  • T to C, chromosome 10 at 23,993,061 bp
  • T to A, chromosome 10 at 43,932,406 bp
  • T to C, chromosome 10 at 127,068,135 bp
  • T to C, chromosome 10 at 129,238,499 bp
  • T to C, chromosome 11 at 53,983,610 bp
  • A to G, chromosome 11 at 58,643,138 bp
  • A to T, chromosome 11 at 66,061,124 bp
  • A to T, chromosome 11 at 73,193,081 bp
  • G to T, chromosome 11 at 75,452,983 bp
  • A to G, chromosome 11 at 78,160,014 bp
  • A to G, chromosome 11 at 95,975,296 bp
  • A to G, chromosome 12 at 37,167,477 bp
  • A to G, chromosome 12 at 57,306,056 bp
  • G to T, chromosome 12 at 80,338,861 bp
  • T to G, chromosome 12 at 80,496,801 bp
  • A to C, chromosome 12 at 81,502,052 bp
  • T to A, chromosome 12 at 104,138,810 bp
  • A to G, chromosome 13 at 12,398,890 bp
  • T to A, chromosome 13 at 96,634,886 bp
  • A to G, chromosome 13 at 97,171,520 bp
  • T to C, chromosome 14 at 11,952,897 bp
  • A to G, chromosome 14 at 50,850,580 bp
  • A to T, chromosome 14 at 118,121,737 bp
  • A to G, chromosome 14 at 122,311,306 bp
  • G to A, chromosome 15 at 12,824,452 bp
  • A to G, chromosome 15 at 66,919,327 bp
  • A to G, chromosome 15 at 76,661,254 bp
  • T to C, chromosome 16 at 22,026,750 bp
  • T to C, chromosome 16 at 22,956,199 bp
  • T to G, chromosome 16 at 32,794,177 bp
  • T to C, chromosome 16 at 38,625,579 bp
  • A to T, chromosome 17 at 28,195,216 bp
  • G to C, chromosome 17 at 47,824,637 bp
  • T to A, chromosome 19 at 34,619,520 bp
  • T to C, chromosome 19 at 41,602,247 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2104 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040108-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.