Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR2149Btlr/Mmmh
Stock Number:
040152-MU
Citation ID:
RRID:MMRRC_040152-MU
Other Names:
R2149 (G1), C57BL/6J-MtgxR2149Btlr
Major Collection:

Strain Information

Kifc2
Name: kinesin family member C2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16581
VEGA: 15
Homologene: 7800
Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Iqgap1
Name: IQ motif containing GTPase activating protein 1
Synonyms: D7Ertd237e, D7Ertd257e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 29875
HGNC: HGNC:6110
Homologene: 74514
Snx4
Name: sorting nexin 4
Synonyms: 1810036H14Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 69150
VEGA: 16
Homologene: 36143
Xpa
Name: xeroderma pigmentosum, complementation group A
Synonyms: Xpac
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22590
Homologene: 37298
Thada
Name: thyroid adenoma associated
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240174
VEGA: 17
Homologene: 75175
Sbno1
Name: strawberry notch 1
Synonyms: sno, 9330180L10Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243272
Homologene: 10055
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 10,199,878 bp
  • C to A, chromosome 1 at 36,812,609 bp
  • A to G, chromosome 1 at 43,742,336 bp
  • C to A, chromosome 1 at 71,263,488 bp
  • T to C, chromosome 1 at 84,907,399 bp
  • T to C, chromosome 1 at 127,793,426 bp
  • A to T, chromosome 1 at 150,388,559 bp
  • T to C, chromosome 1 at 171,767,272 bp
  • T to C, chromosome 1 at 173,641,259 bp
  • T to G, chromosome 1 at 191,345,109 bp
  • C to T, chromosome 2 at 32,034,466 bp
  • T to C, chromosome 2 at 76,729,363 bp
  • A to G, chromosome 2 at 82,258,664 bp
  • A to G, chromosome 2 at 131,143,057 bp
  • A to G, chromosome 2 at 158,195,492 bp
  • T to C, chromosome 3 at 67,474,560 bp
  • C to T, chromosome 3 at 90,089,784 bp
  • T to A, chromosome 3 at 97,792,836 bp
  • T to C, chromosome 3 at 124,409,686 bp
  • T to C, chromosome 4 at 46,183,189 bp
  • G to T, chromosome 4 at 96,641,340 bp
  • C to T, chromosome 4 at 125,126,642 bp
  • C to T, chromosome 4 at 141,408,017 bp
  • T to G, chromosome 4 at 155,905,625 bp
  • A to C, chromosome 5 at 104,455,590 bp
  • A to G, chromosome 5 at 104,776,005 bp
  • T to A, chromosome 5 at 122,443,826 bp
  • T to C, chromosome 5 at 124,402,119 bp
  • T to C, chromosome 5 at 124,599,785 bp
  • T to A, chromosome 5 at 127,462,962 bp
  • A to T, chromosome 5 at 129,596,751 bp
  • T to C, chromosome 6 at 8,155,383 bp
  • T to C, chromosome 6 at 38,336,537 bp
  • T to C, chromosome 6 at 41,356,561 bp
  • T to G, chromosome 6 at 49,439,218 bp
  • C to T, chromosome 6 at 71,844,675 bp
  • C to A, chromosome 6 at 128,646,135 bp
  • G to A, chromosome 7 at 3,844,171 bp
  • A to T, chromosome 7 at 5,675,359 bp
  • A to T, chromosome 7 at 16,159,164 bp
  • T to C, chromosome 7 at 20,028,629 bp
  • A to T, chromosome 7 at 21,052,964 bp
  • A to T, chromosome 7 at 67,531,682 bp
  • T to C, chromosome 7 at 80,762,560 bp
  • T to A, chromosome 7 at 92,625,891 bp
  • A to T, chromosome 7 at 107,036,338 bp
  • T to A, chromosome 7 at 127,786,518 bp
  • C to T, chromosome 7 at 129,379,371 bp
  • C to T, chromosome 7 at 140,638,052 bp
  • CGTG to CGTGTG, chromosome 7 at 141,699,185 bp
  • A to C, chromosome 8 at 45,795,443 bp
  • G to A, chromosome 8 at 83,271,449 bp
  • T to C, chromosome 8 at 110,726,383 bp
  • A to T, chromosome 9 at 20,771,270 bp
  • T to C, chromosome 9 at 38,581,508 bp
  • T to A, chromosome 9 at 53,934,227 bp
  • T to G, chromosome 9 at 58,152,539 bp
  • T to A, chromosome 9 at 62,371,802 bp
  • TCCC to TCC, chromosome 9 at 69,489,754 bp
  • C to A, chromosome 9 at 106,076,929 bp
  • T to C, chromosome 9 at 114,450,648 bp
  • G to A, chromosome 10 at 79,740,275 bp
  • A to T, chromosome 10 at 86,865,040 bp
  • A to T, chromosome 10 at 92,868,874 bp
  • A to G, chromosome 10 at 111,986,689 bp
  • A to G, chromosome 11 at 9,267,508 bp
  • T to A, chromosome 11 at 33,599,424 bp
  • A to G, chromosome 11 at 34,229,472 bp
  • G to T, chromosome 11 at 116,260,867 bp
  • A to G, chromosome 12 at 7,993,059 bp
  • G to A, chromosome 12 at 72,112,161 bp
  • T to C, chromosome 13 at 48,804,689 bp
  • G to A, chromosome 13 at 52,835,496 bp
  • T to A, chromosome 13 at 74,363,757 bp
  • A to G, chromosome 14 at 28,513,317 bp
  • T to C, chromosome 14 at 49,751,571 bp
  • G to T, chromosome 14 at 119,075,345 bp
  • A to T, chromosome 14 at 123,370,017 bp
  • T to A, chromosome 15 at 66,023,729 bp
  • A to T, chromosome 15 at 73,555,060 bp
  • T to C, chromosome 15 at 75,108,543 bp
  • C to A, chromosome 15 at 76,662,221 bp
  • A to G, chromosome 15 at 84,232,168 bp
  • T to C, chromosome 15 at 96,370,835 bp
  • T to C, chromosome 16 at 33,276,937 bp
  • T to C, chromosome 16 at 48,460,672 bp
  • T to A, chromosome 16 at 92,499,207 bp
  • C to T, chromosome 17 at 19,588,963 bp
  • ATTT to ATT, chromosome 17 at 23,290,932 bp
  • T to C, chromosome 17 at 34,514,347 bp
  • C to T, chromosome 17 at 36,877,014 bp
  • T to C, chromosome 17 at 45,633,107 bp
  • A to G, chromosome 17 at 56,417,706 bp
  • T to C, chromosome 17 at 67,773,865 bp
  • T to C, chromosome 17 at 71,273,992 bp
  • C to T, chromosome 17 at 84,441,764 bp
  • A to G, chromosome 17 at 87,986,088 bp
  • T to A, chromosome 18 at 36,532,238 bp
  • C to A, chromosome 18 at 58,102,325 bp
  • T to C, chromosome 18 at 61,157,674 bp
  • A to G, chromosome 19 at 6,068,134 bp
  • A to G, chromosome 19 at 6,226,982 bp
  • A to T, chromosome 19 at 11,649,614 bp
  • A to C, chromosome 19 at 13,838,582 bp
  • A to G, chromosome 19 at 34,021,617 bp
  • T to A, chromosome Y at 10,641,918 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2149 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040152-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.