Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR2155Btlr/Mmmh
Stock Number:
040158-MU
Citation ID:
RRID:MMRRC_040158-MU
Other Names:
R2155 (G1), C57BL/6J-MtgxR2155Btlr
Major Collection:

Strain Information

Nhlh1
Name: nescient helix loop helix 1
Synonyms: Hen1, Nscl, Tal2, Nscl, bHLHa35
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18071
HGNC: HGNC:7817
Homologene: 4089
Slc17a5
Name: solute carrier family 17 (anion/sugar transporter), member 5
Synonyms: 4631416G20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235504
Homologene: 56571
Slamf6
Name: SLAM family member 6
Synonyms: SF2000, NTB-A, KAL1b, KAL1, Ly108
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 30925
Homologene: 49945
Pdzd2
Name: PDZ domain containing 2
Synonyms: 4930537L06Rik, LOC223364, A930022H17Rik, Pdzk3, Gm21706
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68070
Homologene: 23393
Dtx4
Name: deltex 4, E3 ubiquitin ligase
Synonyms: RNF155
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 207521
VEGA: 19
Homologene: 27781
Topors
Name: topoisomerase I binding, arginine/serine-rich
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 106021
Homologene: 4237
Smg7
Name: SMG7 nonsense mediated mRNA decay factor
Synonyms: 9430023P16Rik, Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans)
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226517
Homologene: 32235
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 58,170,751 bp
  • A to C, chromosome 1 at 91,384,623 bp
  • G to T, chromosome 1 at 110,048,864 bp
  • A to G, chromosome 1 at 150,382,335 bp
  • G to A, chromosome 1 at 152,840,313 bp
  • T to A, chromosome 1 at 171,938,008 bp
  • A to G, chromosome 1 at 172,053,957 bp
  • T to C, chromosome 2 at 3,409,253 bp
  • A to T, chromosome 2 at 31,460,349 bp
  • C to T, chromosome 2 at 32,314,937 bp
  • T to A, chromosome 2 at 32,777,028 bp
  • G to A, chromosome 2 at 35,085,354 bp
  • A to T, chromosome 2 at 94,366,642 bp
  • A to G, chromosome 2 at 110,632,796 bp
  • A to G, chromosome 2 at 164,414,423 bp
  • A to C, chromosome 3 at 53,507,587 bp
  • G to A, chromosome 3 at 55,196,721 bp
  • G to T, chromosome 3 at 59,331,680 bp
  • G to A, chromosome 3 at 88,636,044 bp
  • G to A, chromosome 3 at 116,647,830 bp
  • G to T, chromosome 3 at 136,890,450 bp
  • C to T, chromosome 3 at 153,844,685 bp
  • T to A, chromosome 4 at 40,262,790 bp
  • A to G, chromosome 4 at 132,338,049 bp
  • A to T, chromosome 4 at 134,363,180 bp
  • A to T, chromosome 4 at 141,818,039 bp
  • A to C, chromosome 4 at 147,613,458 bp
  • A to C, chromosome 4 at 148,592,491 bp
  • T to C, chromosome 4 at 149,488,203 bp
  • T to C, chromosome 5 at 14,714,295 bp
  • A to T, chromosome 5 at 38,214,120 bp
  • G to T, chromosome 5 at 128,788,165 bp
  • T to C, chromosome 5 at 129,657,152 bp
  • T to C, chromosome 5 at 137,780,005 bp
  • G to C, chromosome 5 at 143,961,256 bp
  • A to C, chromosome 6 at 12,379,633 bp
  • G to T, chromosome 6 at 90,072,459 bp
  • G to A, chromosome 6 at 141,483,914 bp
  • T to C, chromosome 6 at 141,980,944 bp
  • A to G, chromosome 7 at 24,612,645 bp
  • T to C, chromosome 7 at 28,107,203 bp
  • G to T, chromosome 7 at 46,099,357 bp
  • T to A, chromosome 7 at 58,819,745 bp
  • T to C, chromosome 7 at 62,380,792 bp
  • T to A, chromosome 7 at 66,783,414 bp
  • A to T, chromosome 7 at 73,540,768 bp
  • A to G, chromosome 7 at 79,461,720 bp
  • T to C, chromosome 7 at 79,755,924 bp
  • C to G, chromosome 7 at 108,630,777 bp
  • A to G, chromosome 7 at 128,130,391 bp
  • A to T, chromosome 7 at 131,097,575 bp
  • A to C, chromosome 7 at 140,466,591 bp
  • G to A, chromosome 8 at 3,511,787 bp
  • G to A, chromosome 8 at 9,972,766 bp
  • T to A, chromosome 8 at 15,084,555 bp
  • A to G, chromosome 8 at 22,013,584 bp
  • AGAGGAGGAGGAGGAGGAGGAGGAG to AGAGGAGGAGGAGGAGGAGGAG, chromosome 8 at 22,935,647 bp
  • T to C, chromosome 8 at 64,749,770 bp
  • A to T, chromosome 9 at 13,244,908 bp
  • A to T, chromosome 9 at 78,577,173 bp
  • A to T, chromosome 9 at 119,609,448 bp
  • C to T, chromosome 11 at 53,399,619 bp
  • T to C, chromosome 11 at 76,910,173 bp
  • T to C, chromosome 11 at 103,620,459 bp
  • A to T, chromosome 12 at 70,159,480 bp
  • T to A, chromosome 12 at 74,898,456 bp
  • A to T, chromosome 13 at 21,495,657 bp
  • A to T, chromosome 13 at 21,520,589 bp
  • A to T, chromosome 13 at 29,717,832 bp
  • C to A, chromosome 13 at 41,032,544 bp
  • A to G, chromosome 14 at 27,001,477 bp
  • A to G, chromosome 14 at 30,938,071 bp
  • A to T, chromosome 14 at 50,148,697 bp
  • A to G, chromosome 14 at 50,360,682 bp
  • A to T, chromosome 14 at 54,953,794 bp
  • A to G, chromosome 14 at 57,398,423 bp
  • A to G, chromosome 14 at 67,714,838 bp
  • A to T, chromosome 15 at 12,375,793 bp
  • T to C, chromosome 15 at 97,794,063 bp
  • T to A, chromosome 15 at 99,939,785 bp
  • T to A, chromosome 15 at 101,562,611 bp
  • A to G, chromosome 16 at 7,294,082 bp
  • G to T, chromosome 16 at 14,132,429 bp
  • A to G, chromosome 16 at 17,121,396 bp
  • G to T, chromosome 16 at 58,926,123 bp
  • A to G, chromosome 16 at 65,546,991 bp
  • T to A, chromosome 16 at 69,860,597 bp
  • A to T, chromosome 17 at 21,397,708 bp
  • A to G, chromosome 17 at 35,822,209 bp
  • G to T, chromosome 17 at 38,082,180 bp
  • T to A, chromosome 17 at 46,579,617 bp
  • G to A, chromosome 17 at 49,454,358 bp
  • A to T, chromosome 17 at 73,505,432 bp
  • A to T, chromosome 18 at 32,426,220 bp
  • A to T, chromosome 18 at 77,010,941 bp
  • A to G, chromosome 18 at 90,510,381 bp
  • C to A, chromosome 19 at 4,962,469 bp
  • T to C, chromosome 19 at 6,084,429 bp
  • T to A, chromosome 19 at 12,485,282 bp
  • G to T, chromosome 19 at 28,531,302 bp
  • A to G, chromosome 19 at 46,591,546 bp
  • T to A, chromosome 19 at 59,300,421 bp
  • T to C, chromosome X at 136,983,467 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2155 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040158-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.