Strain Name:
C57BL/6J-MtgxR2159Btlr/Mmmh
Stock Number:
040162-MU
Citation ID:
RRID:MMRRC_040162-MU
Other Names:
R2159 (G1), C57BL/6J-MtgxR2159Btlr
Major Collection:

Gene Information

Camk2a
Name: calcium/calmodulin-dependent protein kinase II alpha
Synonyms: alpha-CaMKII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12322
HGNC: HGNC:1460
Homologene: 56577
Foxa3
Name: forkhead box A3
Synonyms: Hnf-3g, Tcf3g, Tcf-3g, Hnf3g
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 15377
HGNC: HGNC:5023
Homologene: 3308
Mga
Name: MAX gene associated
Synonyms: C130042M01Rik, Mga, D030062C11Rik, Mad5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 29808
Homologene: 49351
Cdk2ap1
Name: CDK2 (cyclin-dependent kinase 2)-associated protein 1
Synonyms: DORC1, ST19, p12, Doc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13445
Homologene: 3411
Cwc27
Name: CWC27 spliceosome-associated protein
Synonyms: 3110009E13Rik, NY-CO-10, Sdccag10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 67285
Homologene: 21192
Oxsr1
Name: oxidative-stress responsive 1
Synonyms: 2210022N24Rik, 2810422B09Rik, Osr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 108737
HGNC: HGNC:8508
Homologene: 31288
Sec24a
Name: Sec24 related gene family, member A (S. cerevisiae)
Synonyms: 9430090N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 77371
Homologene: 70131
Serpini1
Name: serine (or cysteine) peptidase inhibitor, clade I, member 1
Synonyms: Spi17, Neuroserpin, Ns, PI12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20713
HGNC: HGNC:8943
Homologene: 21045
Casp3
Name: caspase 3
Synonyms: AC-3, Yama, mldy, Apopain, CPP32, Caspase-3, CC3, A830040C14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12367
HGNC: HGNC:1504
Homologene: 37912
Stim2
Name: stromal interaction molecule 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 116873
Homologene: 32490
Sema4d
Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Synonyms: coll-4, CD100, Semcl2, Semaj, Semacl2, semaphorin H, M-sema G
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20354
Homologene: 21282
Apob
Name: apolipoprotein B
Synonyms: apob-48, apob-100
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238055
HGNC: HGNC:603
Homologene: 328
Tecr
Name: trans-2,3-enoyl-CoA reductase
Synonyms: A230102P12Rik, D17Ertd178e, 2410016D23Rik, SC2, Gpsn2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 106529
HGNC: HGNC:4551
Homologene: 36231
Setd1a
Name: SET domain containing 1A
Synonyms: KMT2F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233904
Homologene: 52251
Prmt5
Name: protein arginine N-methyltransferase 5
Synonyms: Jbp1, Skb1, Jak-binding protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 27374
Homologene: 4454
Btbd1
Name: BTB (POZ) domain containing 1
Synonyms: 1190005H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 83962
HGNC: HGNC:1120
Homologene: 23529
Parm1
Name: prostate androgen-regulated mucin-like protein 1
Synonyms: 2210012L08Rik, 9130213B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231440
Homologene: 41036
Phf10
Name: PHD finger protein 10
Synonyms: 1810055P05Rik, Baf45a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72057
Homologene: 10112
Sp8
Name: trans-acting transcription factor 8
Synonyms: D930049B17Rik, mBtd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 320145
VEGA: 12
Homologene: 18548
Cebpb
Name: CCAAT/enhancer binding protein (C/EBP), beta
Synonyms: NF-IL6, CRP2, C/EBP BETA, Nfil6, NF-M, LAP, C/EBPbeta, IL-6DBP, LIP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12608
HGNC: HGNC:1834
Homologene: 3807
Gp2
Name: glycoprotein 2 (zymogen granule membrane)
Synonyms: 2310037I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67133
HGNC: HGNC:4441
Homologene: 133790
Ccnt2
Name: cyclin T2
Synonyms: CycT2, 2900041I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72949
HGNC: HGNC:1600
Homologene: 14043
Ptp4a3
Name: protein tyrosine phosphatase 4a3
Synonyms: Prl-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 19245
VEGA: 15
HGNC: HGNC:9636
Homologene: 22500
Dnah5
Name: dynein, axonemal, heavy chain 5
Synonyms: b2b1154Clo, b2b1134Clo, b2b1537Clo, b2b3491Clo, b2b1565Clo, Dnahc5, Mdnah5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 110082
HGNC: HGNC:2950
Homologene: 1048
Ttn
Name: titin
Synonyms: shru, L56, mdm, 2310057K23Rik, 2310074I15Rik, 2310036G12Rik, 1100001C23Rik, connectin, D330041I19Rik, D830007G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Rapgef4
Name: Rap guanine nucleotide exchange factor (GEF) 4
Synonyms: Epac2, 6330581N18Rik, 1300003D15Rik, 5730402K07Rik, cAMP-GEFII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56508
Homologene: 4451
Col5a3
Name: collagen, type V, alpha 3
Synonyms: Pro-alpha3(V)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 53867
Homologene: 9253
Srgap3
Name: SLIT-ROBO Rho GTPase activating protein 3
Synonyms: Arhgap14, D130026O08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 259302
Homologene: 56686
Mybpc3
Name: myosin binding protein C, cardiac
Synonyms: cardiac C-protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17868
HGNC: HGNC:7551
Homologene: 215
Ncoa6
Name: nuclear receptor coactivator 6
Synonyms: NRC, ASC-2, ASC2, RAP250, AIB3, PRIP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56406
Homologene: 137337
BC024139
Name: cDNA sequence BC024139
Synonyms: 6230424I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 271278
Homologene: 129974
Vwf
Name: Von Willebrand factor
Synonyms: B130011O06Rik, 6820430P06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 22371
Homologene: 466
Pwwp2b
Name: PWWP domain containing 2B
Synonyms: D930023J19Rik, D7Ertd517e, Pwwp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101631
Homologene: 19056
Eif2ak2
Name: eukaryotic translation initiation factor 2-alpha kinase 2
Synonyms: 2310047A08Rik, IFN-induced and double-stranded RNA-activated kinase, 4732414G15Rik, eIF-2 alpha, IFN- type I-induced and dsRNA-activated kinase, dsRNA-activated kinase, Prkr, Tik, eIF-2 alpha, Pkr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19106
HGNC: HGNC:9437
Homologene: 48134
Gprc6a
Name: G protein-coupled receptor, family C, group 6, member A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 210198
VEGA: 10
Homologene: 17529
Wdpcp
Name: WD repeat containing planar cell polarity effector
Synonyms: AV249152, homoloc-13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216560
Homologene: 9299
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239420
Homologene: 65982
Cpd
Name: carboxypeptidase D
Synonyms: D830034L15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12874
HGNC: HGNC:2301
Homologene: 999
Atp10b
Name: ATPase, class V, type 10B
Synonyms: 5930426O13Rik, 9030605H24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 319767
Homologene: 70969
Olfr70
Name: olfactory receptor 70
Synonyms: MOR262-10, mOR6, GA_x6K02T2N78B-16239704-16240654
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56014
Homologene: 10459
Wdr90
Name: WD repeat domain 90
Synonyms: 3230401M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106618
Homologene: 27066
Vmn2r108
Name: vomeronasal 2, receptor 108
Synonyms: EG627805
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 627805
Homologene: 129678
Ano9
Name: anoctamin 9
Synonyms: Trp53i5, 5430425C04Rik, Tp53i5, Tmem16j
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71345
Homologene: 67083
Tectb
Name: tectorin beta
Synonyms: Tctnb, [b]-tectorin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 21684
Homologene: 7568
Rad51ap2
Name: RAD51 associated protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 209550
VEGA: 12
Homologene: 85245
Gzmd
Name: granzyme D
Synonyms: Ctla-5, Ctla5, CCP5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 14941
HGNC: HGNC:4710
Homologene: 133275
Alg11
Name: asparagine-linked glycosylation 11 (alpha-1,2-mannosyltransferase)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 207958
Homologene: 68893
Nostrin
Name: nitric oxide synthase trafficker
Synonyms: mDaIP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329416
Homologene: 43142
Adam21
Name: a disintegrin and metallopeptidase domain 21
Synonyms: ADAM31
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 56622
HGNC: HGNC:200
Homologene: 68365
Slc22a20
Name: solute carrier family 22 (organic anion transporter), member 20
Synonyms: LOC381203, mOAT6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 381203
Homologene: 87203
Vmn2r8
Name: vomeronasal 2, receptor 8
Synonyms: EG627479
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 627479
Homologene: 129606
Klk1b1
Name: kallikrein 1-related peptidase b1
Synonyms: tissue kallikrein, mK1, mGK-1, Klk1, TK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16623
HGNC: HGNC:6364
Homologene: 68141
Lcmt2
Name: leucine carboxyl methyltransferase 2
Synonyms: Tyw4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329504
Homologene: 19642
Stk36
Name: serine/threonine kinase 36
Synonyms: Fused, 1700112N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 269209
Homologene: 49432
Klhdc10
Name: kelch domain containing 10
Synonyms: 2410127E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 76788
Homologene: 15267
Vmn2r12
Name: vomeronasal 2, receptor 12
Synonyms: Gm6769
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 627569
Homologene: 129606
Cyp21a1
Name: cytochrome P450, family 21, subfamily a, polypeptide 1
Synonyms: 21OH, Oh21-1, 21OHA, 21-hydroxylase, 21-OH, Oh21-1, Cyp21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13079
Homologene: 68063
Psph
Name: phosphoserine phosphatase
Synonyms: PSPase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100678
HGNC: HGNC:9577
Homologene: 31245
Troap
Name: trophinin associated protein
Synonyms: tastin, E130301L11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 78733
VEGA: 15
Homologene: 28697
Ptgir
Name: prostaglandin I receptor (IP)
Synonyms: prostacyclin receptor, IP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19222
HGNC: HGNC:9602
Homologene: 7496
Loxl4
Name: lysyl oxidase-like 4
Synonyms: 4833426I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 67573
Homologene: 12977
Aida
Name: axin interactor, dorsalization associated
Synonyms: 2610208M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 108909
Homologene: 11268
Sftpb
Name: surfactant associated protein B
Synonyms: Sftp-3, Sftp3, SP-B, SF-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20388
Homologene: 456
Olfr1264
Name: olfactory receptor 1264
Synonyms: GA_x6K02T2Q125-51454183-51453257, MOR236-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258206
Homologene: 66365
Dupd1
Name: dual specificity phosphatase and pro isomerase domain containing 1
Synonyms: EG435391
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 435391
VEGA: 14
Homologene: 51120
Vmo1
Name: vitelline membrane outer layer 1 homolog (chicken)
Synonyms: LOC327956
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 327956
Homologene: 45649
Gm2022
Name: predicted pseudogene 2022
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 100039052
Homologene: 103865
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 74,634,737 bp
  • A to G, chromosome 1 at 127,775,154 bp
  • C to T, chromosome 1 at 183,322,379 bp
  • A to G, chromosome 2 at 69,180,922 bp
  • A to G, chromosome 2 at 72,174,881 bp
  • G to A, chromosome 2 at 76,899,327 bp
  • A to T, chromosome 2 at 90,021,538 bp
  • A to T, chromosome 2 at 91,125,370 bp
  • T to A, chromosome 2 at 119,919,643 bp
  • G to A, chromosome 2 at 121,139,285 bp
  • G to T, chromosome 2 at 155,407,713 bp
  • G to T, chromosome 2 at 167,689,253 bp
  • A to G, chromosome 3 at 75,623,944 bp
  • T to C, chromosome 4 at 43,697,110 bp
  • T to C, chromosome 5 at 54,109,814 bp
  • A to G, chromosome 5 at 91,613,064 bp
  • T to A, chromosome 5 at 108,802,303 bp
  • T to A, chromosome 5 at 109,091,474 bp
  • G to A, chromosome 5 at 124,348,604 bp
  • A to T, chromosome 5 at 129,769,387 bp
  • T to C, chromosome 6 at 30,441,764 bp
  • T to C, chromosome 6 at 72,309,786 bp
  • A to G, chromosome 6 at 112,771,378 bp
  • T to C, chromosome 6 at 125,626,341 bp
  • A to G, chromosome 7 at 16,906,869 bp
  • A to G, chromosome 7 at 19,014,184 bp
  • A to T, chromosome 7 at 43,970,433 bp
  • A to T, chromosome 7 at 81,801,056 bp
  • T to C, chromosome 7 at 119,452,284 bp
  • G to A, chromosome 7 at 127,785,489 bp
  • A to T, chromosome 7 at 139,254,928 bp
  • T to C, chromosome 7 at 141,108,117 bp
  • T to G, chromosome 8 at 22,065,845 bp
  • A to G, chromosome 8 at 46,634,288 bp
  • C to T, chromosome 8 at 83,573,019 bp
  • A to G, chromosome 9 at 20,771,310 bp
  • A to C, chromosome 9 at 119,304,814 bp
  • CAAA to CA, chromosome 10 at 51,615,680 bp
  • A to T, chromosome 11 at 21,857,476 bp
  • C to T, chromosome 11 at 43,151,853 bp
  • T to G, chromosome 11 at 51,712,350 bp
  • A to G, chromosome 11 at 70,513,782 bp
  • T to C, chromosome 11 at 76,797,641 bp
  • A to T, chromosome 12 at 8,010,081 bp
  • T to C, chromosome 12 at 11,457,751 bp
  • G to A, chromosome 12 at 81,560,467 bp
  • A to G, chromosome 12 at 87,895,701 bp
  • T to C, chromosome 12 at 118,848,706 bp
  • T to C, chromosome 13 at 51,720,535 bp
  • A to G, chromosome 13 at 104,804,329 bp
  • G to A, chromosome 14 at 21,677,172 bp
  • T to C, chromosome 14 at 54,515,338 bp
  • T to A, chromosome 14 at 56,130,696 bp
  • A to G, chromosome 15 at 28,252,545 bp
  • CCTTTGCGCTT to CCTT, chromosome 15 at 47,741,236 bp
  • A to G, chromosome 15 at 73,752,016 bp
  • T to C, chromosome 15 at 76,121,488 bp
  • G to T, chromosome 15 at 99,077,586 bp
  • C to T, chromosome 17 at 14,952,664 bp
  • C to A, chromosome 17 at 20,469,101 bp
  • T to C, chromosome 17 at 25,851,741 bp
  • C to A, chromosome 17 at 34,802,404 bp
  • A to G, chromosome 17 at 78,874,018 bp
  • G to A, chromosome 18 at 60,957,185 bp
  • T to A, chromosome 19 at 5,981,760 bp
  • C to T, chromosome 19 at 42,600,007 bp
  • C to G, chromosome 19 at 55,180,999 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2159 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040162-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.