Beutler Mutagenetix

Strain Name:
C57BL/6J-MtgxR2201Btlr/Mmmh
Stock Number:
040203-MU
Citation ID:
RRID:MMRRC_040203-MU
Other Names:
R2201 (G1), C57BL/6J-MtgxR2201Btlr
Major Collection:

Gene Information

Myo5a
Name: myosin VA
Synonyms: 9630007J19Rik, MyoVA, flail, MVa, Myo5, Dbv
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Polr3e
Name: polymerase (RNA) III (DNA directed) polypeptide E
Synonyms: Sin, RPC5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 26939
Homologene: 14052
Tln2
Name: talin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 70549
VEGA: 9
Homologene: 56692
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: b2b1562Clo, my, 6030440P17Rik, ne, 8430406N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 242022
Homologene: 18454
Dst
Name: dystonin
Synonyms: A830042E19Rik, 2310001O04Rik, Macf2, bullous pemphigoid antigen 1, BPAG1-n, BPAG1, athetoid, Bpag, ah, bullous pemphigoid antigen 1, Bpag1, nmf339, nmf203
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Ubr5
Name: ubiquitin protein ligase E3 component n-recognin 5
Synonyms: 4432411E13Rik, Edd1, Edd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 70790
VEGA: 15
Homologene: 9295
Bag3
Name: BCL2-associated athanogene 3
Synonyms: Bcl-2-interacting death suppressor, Bis
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 29810
HGNC: HGNC:939
Homologene: 3162
Zfp638
Name: zinc finger protein 638
Synonyms: Zfml, Np220
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 18139
Homologene: 7447
Sec16a
Name: SEC16 homolog A, endoplasmic reticulum export factor
Synonyms: C230052J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 227648
Homologene: 10533
Clic4
Name: chloride intracellular channel 4 (mitochondrial)
Synonyms: mc3s5, mtCLIC, D0Jmb3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 29876
Homologene: 8490
Arap2
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2
Synonyms: Centd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 212285
Homologene: 9064
Ubr7
Name: ubiquitin protein ligase E3 component n-recognin 7 (putative)
Synonyms: 5730410I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 66622
VEGA: 12
Homologene: 11998
Rttn
Name: rotatin
Synonyms: C530033I08Rik, 4921538A15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 246102
VEGA: 18
Homologene: 65275
Hip1
Name: huntingtin interacting protein 1
Synonyms: A930014B11Rik, HIP-1, 2610109B09Rik, E130315I21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 215114
HGNC: HGNC:4913
Homologene: 68463
Pias1
Name: protein inhibitor of activated STAT 1
Synonyms: GBP, 2900068C24Rik, Ddxbp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 56469
VEGA: 9
HGNC: HGNC:2752
Homologene: 22953
C77080
Name: expressed sequence C77080
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 97130
Homologene: 45837
Cc2d2a
Name: coiled-coil and C2 domain containing 2A
Synonyms: b2b1035Clo, 5730509K17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231214
Homologene: 18159
Actr10
Name: ARP10 actin-related protein 10
Synonyms: Arp11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 56444
VEGA: 12
Homologene: 10220
Vps8
Name: VPS8 CORVET complex subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 209018
Homologene: 44592
Npas4
Name: neuronal PAS domain protein 4
Synonyms: LE-PAS, Nxf, Npas4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 225872
VEGA: 19
Homologene: 15333
Fam160a2
Name: family with sequence similarity 160, member A2
Synonyms: 6530415H11Rik, 4632419K20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 74349
Homologene: 75329
Nbeal2
Name: neurobeachin-like 2
Synonyms: 1110014F23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235627
Homologene: 86422
Eng
Name: endoglin
Synonyms: Endo, CD105
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 13805
HGNC: HGNC:3349
Homologene: 92
Dsg2
Name: desmoglein 2
Synonyms: D18Ertd293e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 13511
HGNC: HGNC:3049
Homologene: 1464
Pja2
Name: praja ring finger ubiquitin ligase 2
Synonyms: Neurodap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 224938
Homologene: 32233
Trpm2
Name: transient receptor potential cation channel, subfamily M, member 2
Synonyms: 9830168K16Rik, Trrp7, TRPC7, LTRPC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 28240
Homologene: 20709
Zfhx4
Name: zinc finger homeodomain 4
Synonyms: Zfh-4, C130041O22Rik, Zfh4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 80892
Homologene: 23477
Rapgef4
Name: Rap guanine nucleotide exchange factor (GEF) 4
Synonyms: cAMP-GEFII, 5730402K07Rik, 1300003D15Rik, 6330581N18Rik, Epac2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 56508
Homologene: 4451
4932431P20Rik
Name: RIKEN cDNA 4932431P20 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 114675
Homologene: null
Muc6
Name: mucin 6, gastric
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 353328
HGNC: HGNC:7517
Homologene: 18768
Itga9
Name: integrin alpha 9
Synonyms: 6720458D17Rik, D9Ertd428e, 2610002H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 104099
HGNC: HGNC:6145
Homologene: 1664
Ankrd35
Name: ankyrin repeat domain 35
Synonyms: 4732436F15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 213121
Homologene: 16977
Vmn1r37
Name: vomeronasal 1 receptor 37
Synonyms: V1rc10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 171183
HGNC: null
Homologene: 110800
Stab2
Name: stabilin 2
Synonyms: STAB-2, FEEL-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 192188
Homologene: 23022
Krt6a
Name: keratin 6A
Synonyms: MK6a, mK6[a], Krt2-6a, Krt2-6c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 16687
VEGA: 15
Homologene: 136794
Cacna1s
Name: calcium channel, voltage-dependent, L type, alpha 1S subunit
Synonyms: sj, DHPR alpha1s, muscle dysgenesis, fmd, Cchl1a3, mdg, Cav1.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12292
HGNC: HGNC:1397
Homologene: 37257
A2ml1
Name: alpha-2-macroglobulin like 1
Synonyms: BC048546, Ovos2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 232400
HGNC: null
Homologene: 45969
Tgm7
Name: transglutaminase 7
Synonyms: TGz
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 640543
Homologene: 14192
Megf8
Name: multiple EGF-like-domains 8
Synonyms: Egfl4, b2b1702Clo, m687Ddg, b2b288Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 269878
HGNC: HGNC:3233
Homologene: 15988
Vmn2r72
Name: vomeronasal 2, receptor 72
Synonyms: Vmn2r72-ps, EG244114
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 244114
HGNC: null
Homologene: 115466
Tdrd1
Name: tudor domain containing 1
Synonyms: MTR-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 83561
Homologene: 12850
Ctsj
Name: cathepsin J
Synonyms: Ctsp, CatRLP, CATP, rat gene/Cathepsin L-related protein, Cat P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 26898
VEGA: 13
HGNC: null
Homologene: 137246
Cep85l
Name: centrosomal protein 85-like
Synonyms: Gm9766
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 100038725
VEGA: 10
Homologene: 52598
Sap30
Name: sin3 associated polypeptide
Synonyms: 30kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 60406
Homologene: 2869
Emilin1
Name: elastin microfibril interfacer 1
Synonyms: gp115, EMILIN-1, 5830419M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 100952
Homologene: 5117
Ddx28
Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 28
Synonyms: 2410004K13Rik, Mddx28
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 71986
Homologene: 10164
Col20a1
Name: collagen, type XX, alpha 1
Synonyms: 1700051I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 73368
Homologene: 75138
Kcnt2
Name: potassium channel, subfamily T, member 2
Synonyms: E330038N15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 240776
Homologene: 16121
Dnase2b
Name: deoxyribonuclease II beta
Synonyms: Dlad, DnaseIIb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 56629
Homologene: 1052
Slc5a5
Name: solute carrier family 5 (sodium iodide symporter), member 5
Synonyms: NIS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 114479
Homologene: 37311
Adcy7
Name: adenylate cyclase 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 11513
HGNC: HGNC:238
Homologene: 866
Pfpl
Name: pore forming protein-like
Synonyms: Epcs5, Epcs50
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 56093
VEGA: 19
HGNC: null
Homologene: 130704
Zscan29
Name: zinc finger SCAN domains 29
Synonyms: Zfp690
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 99334
Homologene: 17586
Tmem256
Name: transmembrane protein 256
Synonyms: 1810027O10Rik, 3110009M16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 69186
Homologene: 45148
Vmn2r14
Name: vomeronasal 2, receptor 14
Synonyms: EG231591
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231591
HGNC: null
Homologene: 129606
Fdxr
Name: ferredoxin reductase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 14149
HGNC: HGNC:3642
Homologene: 3033
Nt5m
Name: 5',3'-nucleotidase, mitochondrial
Synonyms: dNT-2, 2010013E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 103850
Homologene: 10622
Fam189a1
Name: family with sequence similarity 189, member A1
Synonyms: 5730507A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 70638
Homologene: 77705
N4bp2l2
Name: NEDD4 binding protein 2-like 2
Synonyms: zag1, 2700092H06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 381695
Homologene: 115380
Dlg2
Name: discs large MAGUK scaffold protein 2
Synonyms: LOC382816, A330103J02Rik, B230218P12Rik, Gm21505, Dlgh2, B330007M19Rik, PSD93, Chapsyn-110
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 23859
HGNC: HGNC:2901
Homologene: 1046
Ttll8
Name: tubulin tyrosine ligase-like family, member 8
Synonyms: 1700019P01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 239591
Homologene: 52766
Slc1a7
Name: solute carrier family 1 (glutamate transporter), member 7
Synonyms: EAAT5, A930031E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 242607
Homologene: 21327
Reep1
Name: receptor accessory protein 1
Synonyms: D6Ertd253e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 52250
Homologene: 41504
Pfn4
Name: profilin family, member 4
Synonyms: 2900024P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 382562
VEGA: 12
Homologene: 20046
Pomc
Name: pro-opiomelanocortin-alpha
Synonyms: POMC, beta-endorphin, BE, Pomc-1, ACTH, adrenal corticotropic hormone, alpha-melanocyte stimulating hormone, beta-MSH, gamma-MSH, gamma-melanocyte stimulating hormone, beta-melanocyte stimulating hormone, alphaMSH, alpha-MSH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 18976
VEGA: 12
HGNC: HGNC:9201
Homologene: 723
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 34,195,921 bp
  • T to C, chromosome 1 at 136,053,059 bp
  • T to A, chromosome 1 at 140,509,441 bp
  • A to T, chromosome 2 at 26,416,243 bp
  • T to C, chromosome 2 at 32,673,740 bp
  • A to G, chromosome 2 at 72,045,189 bp
  • A to T, chromosome 2 at 121,098,581 bp
  • A to C, chromosome 2 at 121,169,402 bp
  • A to G, chromosome 2 at 180,992,422 bp
  • A to G, chromosome 3 at 5,242,289 bp
  • T to A, chromosome 3 at 53,516,573 bp
  • T to C, chromosome 3 at 96,679,248 bp
  • T to A, chromosome 3 at 146,584,688 bp
  • T to C, chromosome 4 at 107,993,006 bp
  • A to G, chromosome 4 at 129,222,639 bp
  • A to G, chromosome 4 at 135,223,539 bp
  • T to C, chromosome 5 at 30,915,692 bp
  • T to C, chromosome 5 at 43,684,033 bp
  • G to A, chromosome 5 at 62,706,685 bp
  • A to G, chromosome 5 at 109,218,832 bp
  • A to T, chromosome 5 at 135,431,730 bp
  • A to T, chromosome 5 at 150,661,608 bp
  • T to A, chromosome 6 at 66,731,894 bp
  • T to C, chromosome 6 at 71,773,294 bp
  • G to C, chromosome 6 at 83,929,518 bp
  • A to T, chromosome 6 at 128,547,305 bp
  • C to T, chromosome 7 at 25,340,745 bp
  • T to A, chromosome 7 at 29,536,525 bp
  • T to A, chromosome 7 at 64,759,393 bp
  • T to C, chromosome 7 at 85,738,236 bp
  • C to T, chromosome 7 at 92,417,337 bp
  • T to C, chromosome 7 at 105,388,191 bp
  • T to C, chromosome 7 at 120,932,242 bp
  • AAAGG to AAAGGAAGG, chromosome 7 at 128,545,769 bp
  • C to T, chromosome 7 at 141,649,810 bp
  • T to C, chromosome 8 at 57,485,472 bp
  • C to T, chromosome 8 at 70,892,458 bp
  • G to A, chromosome 8 at 88,317,978 bp
  • A to G, chromosome 8 at 106,010,574 bp
  • G to T, chromosome 9 at 62,951,855 bp
  • T to C, chromosome 9 at 67,375,757 bp
  • A to G, chromosome 9 at 75,217,943 bp
  • T to A, chromosome 9 at 110,630,250 bp
  • T to C, chromosome 9 at 118,877,115 bp
  • A to T, chromosome 10 at 53,348,731 bp
  • G to A, chromosome 10 at 77,920,471 bp
  • T to A, chromosome 10 at 86,940,639 bp
  • A to G, chromosome 11 at 59,875,915 bp
  • T to C, chromosome 11 at 69,839,445 bp
  • C to A, chromosome 11 at 115,270,382 bp
  • T to C, chromosome 12 at 3,960,275 bp
  • A to G, chromosome 12 at 4,774,382 bp
  • A to G, chromosome 12 at 70,960,021 bp
  • G to A, chromosome 12 at 102,761,505 bp
  • T to C, chromosome 13 at 61,002,549 bp
  • T to C, chromosome 15 at 38,002,299 bp
  • A to G, chromosome 15 at 88,933,953 bp
  • A to G, chromosome 15 at 101,693,171 bp
  • A to G, chromosome 16 at 21,576,757 bp
  • A to T, chromosome 17 at 64,311,167 bp
  • T to A, chromosome 18 at 20,596,054 bp
  • T to A, chromosome 18 at 89,010,943 bp
  • A to T, chromosome 19 at 4,987,364 bp
  • A to C, chromosome 19 at 12,430,479 bp
  • C to A, chromosome 19 at 56,858,661 bp
  • C to A, chromosome 19 at 56,858,662 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2201 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040203-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.