Strain Name:
C57BL/6J-MtgxR2216Btlr/Mmmh
Stock Number:
040218-MU
Citation ID:
RRID:MMRRC_040218-MU
Other Names:
R2216 (G1), C57BL/6J-MtgxR2216Btlr
Major Collection:

Gene Information

Nrp2
Name: neuropilin 2
Synonyms: Npn2, 1110048P06Rik, NP-2, NP2, Npn-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18187
HGNC: HGNC:8005
Homologene: 2875
Sesn2
Name: sestrin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230784
Homologene: 12873
Dysf
Name: dysferlin
Synonyms: 2310004N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 26903
HGNC: HGNC:3097
Homologene: 20748
Cep70
Name: centrosomal protein 70
Synonyms: 6720484E09Rik, C030018L16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68121
Homologene: 11387
Ranbp17
Name: RAN binding protein 17
Synonyms: 4932704E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66011
Homologene: 36409
Wfs1
Name: wolframin ER transmembrane glycoprotein
Synonyms: wolframin, Wolfram syndrome 1 homolog (human)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22393
Homologene: 4380
Gcn1
Name: GCN1 activator of EIF2AK4
Synonyms: GCN1L, Gcn1l1, G431004K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231659
HGNC: HGNC:4199
Homologene: 5887
Tcf12
Name: transcription factor 12
Synonyms: HEB, ALF1, ME1, HTF-4, REB, HTF4, HEBAlt, bHLHb20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 21406
Homologene: 40774
Zcchc8
Name: zinc finger, CCHC domain containing 8
Synonyms: 5730565F05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 70650
Homologene: 32349
Dmxl1
Name: Dmx-like 1
Synonyms: C630007L23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240283
HGNC: HGNC:2937
Homologene: 21136
Cep85
Name: centrosomal protein 85
Synonyms: 2410030J07Rik, Ccdc21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 70012
Homologene: 11254
Dtna
Name: dystrobrevin alpha
Synonyms: alpha-dystrobrevin, Dtn, adbn, A0, 87K protein, a-DB-1, 2210407P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13527
VEGA: 18
HGNC: HGNC:3057
Homologene: 20362
Cyp1a1
Name: cytochrome P450, family 1, subfamily a, polypeptide 1
Synonyms: cytochrome P450 subfamily I, polypeptide 1, P450-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 13076
VEGA: 9
HGNC: HGNC:2595
Homologene: 68062
Bend5
Name: BEN domain containing 5
Synonyms: 2310026E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67621
Homologene: 41574
Tars
Name: threonyl-tRNA synthetase
Synonyms: ThrRS, D15Wsu59e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 110960
VEGA: 15
Homologene: 11852
Prkag1
Name: protein kinase, AMP-activated, gamma 1 non-catalytic subunit
Synonyms: Prkaac
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 19082
VEGA: 15
HGNC: HGNC:9385
Homologene: 99712
Pex19
Name: peroxisomal biogenesis factor 19
Synonyms: Pxf, peroxisome biogenesis factor 19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19298
HGNC: HGNC:9713
Homologene: 134253
Usp54
Name: ubiquitin specific peptidase 54
Synonyms: 4930429G18Rik, C030002J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 78787
Homologene: 90902
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 94109
Homologene: 69536
Cd22
Name: CD22 antigen
Synonyms: Lyb8, Lyb-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12483
HGNC: HGNC:1643
Homologene: 31052
Dennd1c
Name: DENN/MADD domain containing 1C
Synonyms: 4432409M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 70785
VEGA: 17
Homologene: 11773
Pram1
Name: PML-RAR alpha-regulated adaptor molecule 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 378460
Homologene: 12963
Col24a1
Name: collagen, type XXIV, alpha 1
Synonyms: 5430404K19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71355
Homologene: 65061
Reln
Name: reelin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Cmya5
Name: cardiomyopathy associated 5
Synonyms: Myospryn, 2310076E16Rik, 2310076E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 76469
VEGA: 13
Homologene: 137367
Myh15
Name: myosin, heavy chain 15
Synonyms: EG667772
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 667772
VEGA: 16
Homologene: 18929
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: D86 mRNA, fibrocystin L, PKHDL1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 192190
Homologene: 16332
Myo3a
Name: myosin IIIA
Synonyms: 9030416P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 667663
HGNC: HGNC:7601
Homologene: 49486
Prss52
Name: protease, serine 52
Synonyms: 1700049K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 73382
Homologene: 105740
Tnn
Name: tenascin N
Synonyms: tenascin-W, Tnw
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329278
Homologene: 18634
Arnt2
Name: aryl hydrocarbon receptor nuclear translocator 2
Synonyms: bHLHe1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11864
Homologene: 7230
Olfr702
Name: olfactory receptor 702
Synonyms: GA_x6K02T2PBJ9-9202245-9201289, MOR260-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258590
Homologene: 121512
Slc4a9
Name: solute carrier family 4, sodium bicarbonate cotransporter, member 9
Synonyms: D630024F24Rik, AE4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240215
VEGA: 18
Homologene: 23732
Scn5a
Name: sodium channel, voltage-gated, type V, alpha
Synonyms: Nav1.5c, mH1, SkM2, Nav1.5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20271
Homologene: 22738
Rnf112
Name: ring finger protein 112
Synonyms: bfp, Zfp179, neurolastin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 22671
Homologene: 5176
Ube2u
Name: ubiquitin-conjugating enzyme E2U (putative)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381534
Homologene: 17594
Speer2
Name: spermatogenesis associated glutamate (E)-rich protein 2
Synonyms: SPEER-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224318
Homologene: 69402
Kmt2b
Name: lysine (K)-specific methyltransferase 2B
Synonyms: Wbp7, 2610014H22Rik, Mll2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75410
Homologene: 22838
Cep126
Name: centrosomal protein 126
Synonyms: AK129341
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 234915
VEGA: 9
Homologene: 28313
Nebl
Name: nebulette
Synonyms: 1200007O21Rik, D830029A09Rik, A630080F05Rik, Lnebl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74103
Homologene: 128431
Mybpc2
Name: myosin binding protein C, fast-type
Synonyms: Fast-type C-protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233199
HGNC: HGNC:7550
Homologene: 3331
Thsd7a
Name: thrombospondin, type I, domain containing 7A
Synonyms: LOC330267
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330267
Homologene: 46582
Itga1
Name: integrin alpha 1
Synonyms: Vla1, CD49A, E130012M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 109700
VEGA: 13
HGNC: HGNC:6134
Homologene: 57137
Masp1
Name: mannan-binding lectin serine peptidase 1
Synonyms: Crarf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 17174
VEGA: 16
HGNC: HGNC:6901
Homologene: 88793
Vil1
Name: villin 1
Synonyms: Villin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22349
Homologene: 5169
Pcnx2
Name: pecanex homolog 2
Synonyms: E330039K12Rik, Pcnxl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 270109
HGNC: HGNC:8736
Homologene: 8987
Trim40
Name: tripartite motif-containing 40
Synonyms: LOC240093, LOC195359, LOC333872
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 195359
Homologene: 34709
Olfr513
Name: olfactory receptor 513
Synonyms: MOR195-1, GA_x6K02T2PBJ9-11084889-11085818
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258718
Homologene: 64908
Itga2b
Name: integrin alpha 2b
Synonyms: GP IIb, CD41, platelet glycoprotein IIb, GpIIb, alphaIIb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16399
HGNC: HGNC:6138
Homologene: 37304
Hdac9
Name: histone deacetylase 9
Synonyms: Hdac7b, Mitr, HDRP, D030072B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 79221
Homologene: 128578
Parp14
Name: poly (ADP-ribose) polymerase family, member 14
Synonyms: collaborator of Stat6, CoaSt6, 1600029O10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 547253
Homologene: 19697
Zfp946
Name: zinc finger protein 946
Synonyms: 1300003B13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 74149
Tnnt3
Tmem132b
Name: transmembrane protein 132B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 208151
Homologene: 28135
Gpbar1
Name: G protein-coupled bile acid receptor 1
Synonyms: M-BAR, BG37, GPR131, TGR5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227289
Homologene: 18125
Trp53tg5
Name: transformation related protein 53 target 5
Synonyms: 1700126L10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 73603
Homologene: 49386
Pramel18
Name: PRAME like 18
Synonyms: Gm12800
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381535
Klra7
Name: killer cell lectin-like receptor, subfamily A, member 7
Synonyms: Ly49g1, LGL-1, Ly49g, Ly-49G.2, Ly49g2, Ly-49G.1, Ly-49G.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16638
Homologene: 110821
Slc5a6
Name: solute carrier family 5 (sodium-dependent vitamin transporter), member 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 330064
Homologene: 23277
E130304F04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Aipl1
Name: aryl hydrocarbon receptor-interacting protein-like 1
Synonyms: A930007I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 114230
HGNC: HGNC:359
Homologene: 22806
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 62,762,918 bp
  • C to G, chromosome 1 at 74,278,894 bp
  • G to T, chromosome 1 at 74,425,679 bp
  • T to A, chromosome 1 at 160,126,317 bp
  • T to C, chromosome 1 at 172,129,889 bp
  • T to A, chromosome 2 at 17,348,882 bp
  • A to C, chromosome 2 at 22,577,771 bp
  • T to C, chromosome 2 at 164,471,306 bp
  • T to C, chromosome 3 at 145,314,981 bp
  • T to C, chromosome 4 at 100,532,168 bp
  • T to A, chromosome 4 at 101,910,060 bp
  • A to G, chromosome 4 at 111,448,590 bp
  • T to C, chromosome 4 at 132,497,942 bp
  • T to C, chromosome 4 at 134,131,430 bp
  • T to C, chromosome 5 at 22,048,005 bp
  • T to G, chromosome 5 at 31,039,335 bp
  • T to A, chromosome 5 at 36,967,220 bp
  • T to A, chromosome 5 at 115,593,661 bp
  • A to G, chromosome 5 at 123,707,403 bp
  • T to A, chromosome 5 at 125,698,558 bp
  • A to T, chromosome 6 at 12,337,268 bp
  • G to A, chromosome 6 at 84,207,245 bp
  • T to C, chromosome 6 at 130,228,586 bp
  • G to A, chromosome 7 at 30,574,065 bp
  • G to T, chromosome 7 at 30,867,046 bp
  • C to T, chromosome 7 at 44,512,500 bp
  • T to A, chromosome 7 at 84,275,351 bp
  • T to A, chromosome 7 at 106,823,998 bp
  • C to T, chromosome 7 at 108,755,612 bp
  • A to G, chromosome 7 at 142,512,564 bp
  • T to A, chromosome 8 at 17,027,339 bp
  • C to T, chromosome 8 at 125,888,077 bp
  • G to A, chromosome 9 at 8,120,678 bp
  • T to A, chromosome 9 at 57,702,069 bp
  • A to T, chromosome 9 at 72,109,756 bp
  • T to A, chromosome 9 at 99,275,570 bp
  • G to T, chromosome 9 at 119,485,612 bp
  • T to C, chromosome 9 at 119,513,085 bp
  • A to T, chromosome 11 at 33,481,125 bp
  • A to G, chromosome 11 at 61,452,279 bp
  • G to T, chromosome 11 at 72,031,446 bp
  • T to A, chromosome 11 at 102,467,866 bp
  • A to T, chromosome 12 at 34,429,517 bp
  • A to T, chromosome 13 at 93,093,495 bp
  • A to T, chromosome 13 at 114,997,029 bp
  • A to G, chromosome 13 at 119,714,215 bp
  • A to T, chromosome 14 at 20,561,840 bp
  • T to C, chromosome 14 at 64,113,593 bp
  • A to G, chromosome 15 at 11,389,708 bp
  • A to T, chromosome 15 at 44,573,895 bp
  • A to T, chromosome 15 at 98,815,946 bp
  • T to C, chromosome 16 at 23,492,055 bp
  • T to C, chromosome 16 at 35,857,205 bp
  • C to G, chromosome 16 at 49,165,838 bp
  • T to C, chromosome 16 at 69,858,842 bp
  • A to T, chromosome 17 at 22,454,716 bp
  • C to T, chromosome 17 at 33,641,284 bp
  • T to C, chromosome 17 at 36,888,983 bp
  • C to T, chromosome 17 at 57,074,492 bp
  • A to G, chromosome 18 at 23,569,565 bp
  • A to G, chromosome 18 at 36,530,745 bp
  • G to A, chromosome 18 at 49,893,923 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2216 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040218-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.