Strain Name:
C57BL/6J-MtgxR2224Btlr/Mmmh
Stock Number:
040225-MU
Citation ID:
RRID:MMRRC_040225-MU
Other Names:
R2224 (G1), C57BL/6J-MtgxR2224Btlr
Major Collection:

Gene Information

Met
Name: met proto-oncogene
Synonyms: Par4, c-Met, HGF receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206
Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: T-type Cav3.2, Cav3.2, alpha13.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Odc1
Name: ornithine decarboxylase, structural 1
Synonyms: ODC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 18263
VEGA: 12
HGNC: HGNC:8109
Homologene: 1906
Spns1
Name: spinster homolog 1
Synonyms: 2210013K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73658
Homologene: 41530
Ppp1r9a
Name: protein phosphatase 1, regulatory subunit 9A
Synonyms: Neurabin I, 2810430P21Rik, NRB, 4930518N04Rik, neurabin-I, A230094E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243725
Homologene: 14247
Sirt2
Name: sirtuin 2
Synonyms: Sir2l, 5730427M03Rik, SIR2L2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 64383
Homologene: 40823
Pard3
Name: par-3 family cell polarity regulator
Synonyms: ASIP, PAR-3, D8Ertd580e, Par3, Pard3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 93742
Homologene: 10489
Rnmt
Name: RNA (guanine-7-) methyltransferase
Synonyms: 2610002P10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 67897
Homologene: 2816
Exo1
Name: exonuclease 1
Synonyms: Msa, 5730442G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 26909
HGNC: HGNC:3511
Homologene: 31352
Chd9
Name: chromodomain helicase DNA binding protein 9
Synonyms: 1810014J18Rik, AD013, 9030205D12Rik, A330063D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 109151
Homologene: 11844
Btbd9
Name: BTB (POZ) domain containing 9
Synonyms: 1700023F20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224671
VEGA: 17
Homologene: 14995
Brwd1
Name: bromodomain and WD repeat domain containing 1
Synonyms: repro5, Wdr9, D530019K20Rik, 5330419I02Rik, G1-403-16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 93871
Homologene: 23130
Fbxo21
Name: F-box protein 21
Synonyms: 2810425J22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231670
Homologene: 14145
Wdr3
Name: WD repeat domain 3
Synonyms: D030020G18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 269470
Homologene: 4937
Sfxn1
Name: sideroflexin 1
Synonyms: 2810002O05Rik, A930015P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 14057
VEGA: 13
Homologene: 41494
Aen
Name: apoptosis enhancing nuclease
Synonyms: Isg20l1, 2700083B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68048
Homologene: 23382
Mrgpra1
Name: MAS-related GPR, member A1
Synonyms: MrgA1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233221
Homologene: 79615
Pcx
Name: pyruvate carboxylase
Synonyms: Pc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18563
VEGA: 19
HGNC: HGNC:8636
Homologene: 5422
Nbeal2
Name: neurobeachin-like 2
Synonyms: 1110014F23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235627
Homologene: 86422
Il17f
Name: interleukin 17F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 257630
Homologene: 17115
Ddx41
Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 41
Synonyms: 2900024F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 72935
VEGA: 13
Homologene: 9431
Mtus1
Name: mitochondrial tumor suppressor 1
Synonyms: MD44, B430305I03Rik, Atip1, MTSG1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102103
Homologene: 100292
Map4k3
Name: mitogen-activated protein kinase kinase kinase kinase 3
Synonyms: 9530052P13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 225028
VEGA: 17
HGNC: HGNC:6865
Homologene: 2683
Eea1
Name: early endosome antigen 1
Synonyms: B230358H09Rik, A430109M19Rik, ZFYVE2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216238
VEGA: 10
HGNC: HGNC:3185
Homologene: 37822
Ar
Name: androgen receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 11835
HGNC: HGNC:644
Homologene: 28
Tns2
Name: tensin 2
Synonyms: nph, Tenc1, nep
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Fyb
Name: FYN binding protein
Synonyms: ADAP, B630013F22Rik, FYB-120/130
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 23880
HGNC: HGNC:4036
Homologene: 22664
Nkpd1
Name: NTPase, KAP family P-loop domain containing 1
Synonyms: 2310015G09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 69547
Homologene: 18876
Mgam
Name: maltase-glucoamylase
Synonyms: 6030407P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232714
HGNC: HGNC:7043
Homologene: 130099
Vmn2r100
Name: vomeronasal 2, receptor 100
Synonyms: EG627537
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 627537
Homologene: 129750
Brinp3
Name: bone morphogenetic protein/retinoic acid inducible neural specific 3
Synonyms: Fam5c, B830045N13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 215378
Homologene: 17786
Mrgprx2
Name: MAS-related GPR, member X2
Synonyms: Mrgprb10, MrgB10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243978
Homologene: 24986
Atg9b
Name: autophagy related 9B
Synonyms: eONE, Nos3as, Apg9l2, LOC213948
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 213948
Homologene: 72638
BC051142
Name: cDNA sequence BC051142
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 407788
Homologene: 128168
Ahnak
Name: AHNAK nucleoprotein (desmoyokin)
Synonyms: 2310047C17Rik, DY6, 1110004P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 66395
VEGA: 19
HGNC: HGNC:347
Homologene: 67425
Dis3l
Name: DIS3 like exosome 3'-5' exoribonuclease
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 213550
Homologene: 15797
Zbtb4
Name: zinc finger and BTB domain containing 4
Synonyms: 9230111I22Rik, 2310026P19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75580
Homologene: 10846
Emcn
Name: endomucin
Synonyms: 0610012K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 59308
Homologene: 9438
Prpf40b
Name: pre-mRNA processing factor 40B
Synonyms: 2610317D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 54614
Homologene: 81828
Naalad2
Name: N-acetylated alpha-linked acidic dipeptidase 2
Synonyms: NAADALASE2, GCP3, Folh1b, D9Ertd285e, NAALADASE2, GCPIII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72560
Homologene: 3294
Vmn2r108
Name: vomeronasal 2, receptor 108
Synonyms: EG627805
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 627805
Homologene: 129678
Nin
Name: ninein
Synonyms: 3110068G20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 18080
Homologene: 40632
Arsb
Name: arylsulfatase B
Synonyms: As-1s, As-1t, Asr-1, Ast-1, 1110007C02Rik, As-1, As-1r, As1-s, As1, As1-r, As1-t
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 11881
VEGA: 13
HGNC: HGNC:714
Homologene: 73870
Olfr1024
Name: olfactory receptor 1024
Synonyms: MOR197-1, GA_x6K02T2Q125-47384320-47383337
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 257900
Homologene: 128271
Ccdc33
Name: coiled-coil domain containing 33
Synonyms: 4930535E21Rik, LOC382077
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382077
Homologene: 19641
Fam75d3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Hdac9
Name: histone deacetylase 9
Synonyms: HDRP, Mitr, D030072B18Rik, Hdac7b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 79221
Homologene: 128578
Abcc1
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 1
Synonyms: MRP, Mdrap, Mrp1, Abcc1b, Abcc1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 17250
HGNC: HGNC:51
Homologene: 133779
Inpp1
Name: inositol polyphosphate-1-phosphatase
Synonyms: 2300002C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16329
HGNC: HGNC:6071
Homologene: 1655
Endou
Name: endonuclease, polyU-specific
Synonyms: Tcl-30, Pp11r
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 19011
VEGA: 15
Homologene: 48394
Cep250
Name: centrosomal protein 250
Synonyms: Inmp, B230210E21Rik, Cep2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16328
HGNC: HGNC:1859
Homologene: 38286
Olfr1200
Name: olfactory receptor 1200
Synonyms: GA_x6K02T2Q125-50243231-50242221, MOR225-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 257887
Homologene: 123770
Cyp2c68
Name: cytochrome P450, family 2, subfamily c, polypeptide 68
Synonyms: 9030012A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 433247
Homologene: 74936
Fbxw20
Name: F-box and WD-40 domain protein 20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 434440
Homologene: 110776
Gm4953
Name: predicted pseudogene 4953
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240853
Gm17661
Name: predicted gene, 17661
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Olfr1377
Name: olfactory receptor 1377
Synonyms: GA_x6K02T2QP88-4453480-4452557, MOR129-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258913
Homologene: 64929
Uqcrc2
Name: ubiquinol cytochrome c reductase core protein 2
Synonyms: 1500004O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67003
Homologene: 37764
Fpr3
Name: formyl peptide receptor 3
Synonyms: Fprl1, Fpr-rs1, LXA4-R, Lxa4r
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14294
HGNC: HGNC:3828
Dgkk
Name: diacylglycerol kinase kappa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 331374
Homologene: 45867
Hoxa10
Name: homeobox A10
Synonyms: Hoxa-10, Hox-1.8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 15395
HGNC: HGNC:5100
Homologene: 7365
Tex45
Name: testis expressed 45
Synonyms: 1700019B03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 76406
Homologene: 52367
Mup18
Name: major urinary protein 18
Synonyms: Gm12561
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100048884
Homologene: 74304
Leap2
Name: liver-expressed antimicrobial peptide 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 259301
Homologene: 14198
Gm4985
Name: predicted gene 4985
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245376
CU019598.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 20,779,375 bp
  • T to A, chromosome 1 at 52,790,131 bp
  • C to T, chromosome 1 at 146,901,920 bp
  • G to A, chromosome 1 at 159,168,524 bp
  • A to G, chromosome 1 at 175,886,688 bp
  • T to A, chromosome 2 at 85,904,755 bp
  • A to T, chromosome 2 at 88,767,586 bp
  • GG to GGG, chromosome 2 at 90,917,708 bp
  • A to C, chromosome 2 at 155,991,817 bp
  • A to G, chromosome 3 at 100,146,791 bp
  • T to C, chromosome 3 at 137,404,017 bp
  • G to T, chromosome 4 at 61,671,891 bp
  • A to G, chromosome 5 at 24,386,395 bp
  • T to G, chromosome 5 at 118,008,123 bp
  • G to T, chromosome 6 at 5,154,074 bp
  • A to G, chromosome 6 at 17,563,722 bp
  • G to T, chromosome 6 at 40,764,274 bp
  • T to C, chromosome 6 at 52,232,636 bp
  • A to T, chromosome 7 at 19,519,820 bp
  • A to G, chromosome 7 at 28,772,212 bp
  • A to G, chromosome 7 at 47,335,106 bp
  • T to A, chromosome 7 at 48,482,860 bp
  • C to A, chromosome 7 at 78,902,451 bp
  • T to A, chromosome 7 at 120,641,714 bp
  • G to A, chromosome 7 at 126,371,165 bp
  • A to G, chromosome 8 at 3,479,249 bp
  • C to T, chromosome 8 at 41,082,775 bp
  • A to C, chromosome 8 at 91,011,285 bp
  • A to G, chromosome 8 at 127,359,776 bp
  • A to T, chromosome 9 at 18,376,533 bp
  • A to G, chromosome 9 at 58,082,022 bp
  • C to T, chromosome 9 at 64,310,162 bp
  • G to C, chromosome 9 at 109,233,582 bp
  • T to C, chromosome 9 at 110,629,756 bp
  • T to A, chromosome 10 at 96,020,012 bp
  • T to C, chromosome 11 at 50,985,232 bp
  • A to G, chromosome 11 at 53,422,807 bp
  • C to T, chromosome 11 at 69,776,358 bp
  • A to G, chromosome 12 at 17,547,335 bp
  • C to T, chromosome 12 at 34,407,802 bp
  • A to T, chromosome 12 at 70,061,230 bp
  • A to G, chromosome 13 at 54,085,517 bp
  • G to A, chromosome 13 at 55,531,401 bp
  • T to A, chromosome 13 at 59,703,715 bp
  • T to A, chromosome 13 at 93,794,171 bp
  • A to G, chromosome 15 at 6,652,383 bp
  • G to A, chromosome 15 at 97,719,499 bp
  • C to T, chromosome 15 at 99,303,291 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • T to G, chromosome 16 at 14,472,068 bp
  • A to T, chromosome 16 at 96,016,102 bp
  • T to A, chromosome 17 at 17,971,193 bp
  • A to G, chromosome 17 at 19,522,372 bp
  • A to T, chromosome 17 at 20,481,033 bp
  • T to C, chromosome 17 at 25,385,943 bp
  • C to A, chromosome 17 at 30,527,346 bp
  • C to A, chromosome 17 at 34,448,763 bp
  • A to T, chromosome 17 at 80,630,454 bp
  • C to T, chromosome 18 at 68,305,783 bp
  • T to C, chromosome 19 at 4,617,998 bp
  • G to A, chromosome 19 at 9,012,991 bp
  • A to G, chromosome 19 at 39,735,582 bp
  • T to A, chromosome X at 6,875,248 bp
  • T to A, chromosome X at 23,958,934 bp
  • A to T, chromosome X at 34,237,275 bp
  • T to A, chromosome X at 98,151,331 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2224 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040225-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.