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Strain Name:
C57BL/6J-MtgxR2239Btlr/Mmmh
Stock Number:
040239-MU
Citation ID:
RRID:MMRRC_040239-MU
Other Names:
R2239 (G1), C57BL/6J-MtgxR2239Btlr
Major Collection:

Strain Information

Ltbp3
Name: latent transforming growth factor beta binding protein 3
Synonyms: Ltbp2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16998
VEGA: 19
HGNC: HGNC:6716
Homologene: 7405
Eomes
Name: eomesodermin
Synonyms: Tbr2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13813
HGNC: HGNC:3372
Homologene: 3971
Tet1
Name: tet methylcytosine dioxygenase 1
Synonyms: 2510010B09Rik, D10Ertd17e, Cxxc6, BB001228
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52463
Homologene: 12735
Map7
Name: microtubule-associated protein 7
Synonyms: E-MAP-115, mste, ste, mshi, Mtap7
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17761
VEGA: 10
HGNC: HGNC:6869
Homologene: 20851
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Rgs3
Name: regulator of G-protein signaling 3
Synonyms: 4930506N09Rik, C2pa, PDZ-RGS3, RGS3S, C2PA-RGS3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 50780
HGNC: HGNC:9999
Homologene: 32440
Cilk1
Name: ciliogenesis associated kinase 1
Synonyms: 2210420N10Rik, Ick
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56542
Homologene: 69218
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 36,505,678 bp
  • C to A, chromosome 1 at 46,201,184 bp
  • T to C, chromosome 1 at 132,583,022 bp
  • T to A, chromosome 1 at 139,456,846 bp
  • T to C, chromosome 1 at 161,063,282 bp
  • C to A, chromosome 1 at 188,576,214 bp
  • T to A, chromosome 2 at 62,268,646 bp
  • T to A, chromosome 2 at 62,690,377 bp
  • G to A, chromosome 2 at 75,676,554 bp
  • T to C, chromosome 2 at 87,949,397 bp
  • T to C, chromosome 2 at 110,012,393 bp
  • T to A, chromosome 3 at 145,358,508 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • A to T, chromosome 4 at 62,625,887 bp
  • G to A, chromosome 4 at 113,546,536 bp
  • G to A, chromosome 4 at 126,368,522 bp
  • A to C, chromosome 4 at 134,202,205 bp
  • A to G, chromosome 5 at 73,108,547 bp
  • T to A, chromosome 5 at 75,181,508 bp
  • T to C, chromosome 5 at 76,559,710 bp
  • T to G, chromosome 5 at 88,282,554 bp
  • T to C, chromosome 5 at 123,803,670 bp
  • A to G, chromosome 5 at 136,408,041 bp
  • G to A, chromosome 5 at 137,457,837 bp
  • G to A, chromosome 5 at 142,474,865 bp
  • A to G, chromosome 7 at 5,097,602 bp
  • G to A, chromosome 7 at 13,801,372 bp
  • A to T, chromosome 7 at 44,665,183 bp
  • A to G, chromosome 7 at 141,237,692 bp
  • T to C, chromosome 8 at 24,646,287 bp
  • A to C, chromosome 8 at 54,602,629 bp
  • T to C, chromosome 8 at 95,769,521 bp
  • C to A, chromosome 8 at 104,125,672 bp
  • C to A, chromosome 8 at 107,056,361 bp
  • G to A, chromosome 9 at 21,142,533 bp
  • G to A, chromosome 9 at 21,211,268 bp
  • T to C, chromosome 9 at 78,155,336 bp
  • G to A, chromosome 9 at 111,690,122 bp
  • A to G, chromosome 9 at 118,482,291 bp
  • C to T, chromosome 9 at 122,771,147 bp
  • A to T, chromosome 10 at 20,278,282 bp
  • T to C, chromosome 10 at 62,879,734 bp
  • A to G, chromosome 10 at 80,530,988 bp
  • A to G, chromosome 11 at 23,876,378 bp
  • A to G, chromosome 11 at 59,589,271 bp
  • A to T, chromosome 11 at 101,078,463 bp
  • G to T, chromosome 11 at 106,202,134 bp
  • A to G, chromosome 11 at 120,563,282 bp
  • C to A, chromosome 12 at 10,375,558 bp
  • A to G, chromosome 12 at 73,285,114 bp
  • T to A, chromosome 12 at 75,007,719 bp
  • T to C, chromosome 12 at 91,347,567 bp
  • A to G, chromosome 12 at 100,355,001 bp
  • T to A, chromosome 13 at 47,073,755 bp
  • G to T, chromosome 13 at 54,575,149 bp
  • A to C, chromosome 13 at 111,748,944 bp
  • A to G, chromosome 14 at 20,739,387 bp
  • A to G, chromosome 14 at 49,306,203 bp
  • A to G, chromosome 14 at 57,455,504 bp
  • A to T, chromosome 14 at 72,574,339 bp
  • A to T, chromosome 14 at 78,299,981 bp
  • T to C, chromosome 15 at 79,252,947 bp
  • C to T, chromosome 15 at 99,954,864 bp
  • T to C, chromosome 16 at 14,443,339 bp
  • T to G, chromosome 16 at 30,338,422 bp
  • T to A, chromosome 16 at 44,820,897 bp
  • T to A, chromosome 16 at 59,329,375 bp
  • T to A, chromosome 17 at 71,310,224 bp
  • A to C, chromosome 17 at 75,547,772 bp
  • C to A, chromosome 19 at 5,751,523 bp
  • T to A, chromosome 19 at 6,337,590 bp
  • T to C, chromosome 19 at 11,828,450 bp
  • T to C, chromosome 19 at 13,234,721 bp
  • A to T, chromosome 19 at 36,437,354 bp
  • A to G, chromosome 19 at 42,078,032 bp
  • T to C, chromosome 19 at 46,163,618 bp
  • T to C, chromosome X at 18,199,237 bp
  • G to T, chromosome X at 49,493,405 bp
  • G to T, chromosome X at 57,066,504 bp
  • A to G, chromosome X at 153,833,676 bp
  • A to G, chromosome X at 160,541,412 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2239 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040239-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.