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Strain Name:
C57BL/6J-MtgxR2259Btlr/Mmmh
Stock Number:
040259-MU
Citation ID:
RRID:MMRRC_040259-MU
Other Names:
R2259 (G1), C57BL/6J-MtgxR2259Btlr
Major Collection:

Strain Information

Sod1
Name: superoxide dismutase 1, soluble
Synonyms: CuZnSOD, SODC, Cu/Zn-SOD, Cu(2+)-Zn2+ superoxide dismutase, Sod-1, Ipo1, Ipo-1, B430204E11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 20655
VEGA: 16
Homologene: 392
Npy2r
Name: neuropeptide Y receptor Y2
Synonyms: NPY-Y2 receptor
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18167
HGNC: HGNC:7957
Homologene: 701
Ift52
Name: intraflagellar transport 52
Synonyms: NGD5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 245866
Homologene: 9335
1190005I06Rik
Name: RIKEN cDNA 1190005I06 gene
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 68918
Homologene: 18858
Dlg4
Name: discs large MAGUK scaffold protein 4
Synonyms: SAP90, PSD-95, SAP90A, PSD95, Dlgh4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13385
HGNC: HGNC:2903
Homologene: 1047
Trip10
Name: thyroid hormone receptor interactor 10
Synonyms: Cip4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 106628
VEGA: 17
Homologene: 99728
E2f7
Name: E2F transcription factor 7
Synonyms: A630014C11Rik, D10Ertd739e, E2F7
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52679
Homologene: 18685
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 5,916,658 bp
  • A to G, chromosome 1 at 30,804,343 bp
  • C to T, chromosome 1 at 58,729,121 bp
  • T to A, chromosome 1 at 174,502,932 bp
  • T to A, chromosome 1 at 185,221,859 bp
  • G to A, chromosome 2 at 25,243,644 bp
  • A to C, chromosome 2 at 118,455,783 bp
  • A to G, chromosome 2 at 126,758,568 bp
  • A to T, chromosome 2 at 139,951,506 bp
  • T to A, chromosome 2 at 156,335,272 bp
  • A to T, chromosome 2 at 158,204,711 bp
  • A to G, chromosome 2 at 163,028,093 bp
  • A to T, chromosome 2 at 169,886,406 bp
  • A to G, chromosome 3 at 30,896,295 bp
  • T to A, chromosome 3 at 63,697,977 bp
  • G to T, chromosome 3 at 82,541,354 bp
  • T to C, chromosome 3 at 87,754,286 bp
  • A to G, chromosome 3 at 87,800,452 bp
  • T to C, chromosome 3 at 144,846,381 bp
  • A to G, chromosome 3 at 159,615,571 bp
  • A to T, chromosome 4 at 43,182,780 bp
  • A to G, chromosome 4 at 43,570,107 bp
  • C to T, chromosome 5 at 122,882,416 bp
  • T to C, chromosome 5 at 127,504,924 bp
  • T to G, chromosome 5 at 129,112,311 bp
  • C to A, chromosome 5 at 134,729,654 bp
  • C to T, chromosome 5 at 135,337,614 bp
  • C to A, chromosome 5 at 137,520,332 bp
  • G to A, chromosome 6 at 29,438,666 bp
  • A to G, chromosome 6 at 42,591,430 bp
  • A to G, chromosome 6 at 48,596,530 bp
  • C to A, chromosome 6 at 83,197,586 bp
  • T to C, chromosome 7 at 4,128,184 bp
  • T to A, chromosome 7 at 4,519,406 bp
  • C to T, chromosome 7 at 4,943,916 bp
  • A to T, chromosome 7 at 6,570,023 bp
  • C to T, chromosome 7 at 26,309,035 bp
  • C to A, chromosome 7 at 29,019,741 bp
  • G to A, chromosome 7 at 31,053,878 bp
  • T to C, chromosome 7 at 44,471,411 bp
  • T to C, chromosome 7 at 44,541,484 bp
  • C to T, chromosome 7 at 79,711,589 bp
  • T to A, chromosome 7 at 84,940,911 bp
  • T to A, chromosome 7 at 86,585,884 bp
  • T to G, chromosome 7 at 98,069,499 bp
  • A to T, chromosome 7 at 101,484,567 bp
  • T to A, chromosome 7 at 104,411,504 bp
  • T to C, chromosome 7 at 127,273,477 bp
  • T to A, chromosome 7 at 134,917,667 bp
  • A to G, chromosome 7 at 141,791,008 bp
  • A to G, chromosome 8 at 46,837,833 bp
  • A to T, chromosome 8 at 120,634,260 bp
  • A to G, chromosome 9 at 27,056,072 bp
  • T to C, chromosome 9 at 38,976,000 bp
  • C to A, chromosome 9 at 44,405,238 bp
  • G to T, chromosome 9 at 44,881,142 bp
  • A to G, chromosome 9 at 67,953,860 bp
  • C to T, chromosome 9 at 79,774,164 bp
  • C to T, chromosome 9 at 114,443,032 bp
  • A to G, chromosome 10 at 27,031,127 bp
  • T to G, chromosome 10 at 110,746,343 bp
  • A to G, chromosome 11 at 43,172,745 bp
  • G to A, chromosome 11 at 43,189,613 bp
  • G to A, chromosome 11 at 66,108,301 bp
  • T to C, chromosome 11 at 70,031,370 bp
  • T to A, chromosome 11 at 72,457,268 bp
  • C to T, chromosome 11 at 72,900,633 bp
  • T to G, chromosome 11 at 77,476,342 bp
  • T to A, chromosome 11 at 82,917,799 bp
  • T to C, chromosome 11 at 97,664,976 bp
  • T to A, chromosome 11 at 98,690,964 bp
  • T to G, chromosome 11 at 99,915,018 bp
  • A to G, chromosome 11 at 102,885,298 bp
  • T to A, chromosome 11 at 114,751,928 bp
  • T to C, chromosome 11 at 116,306,411 bp
  • T to C, chromosome 11 at 116,841,314 bp
  • T to G, chromosome 11 at 119,543,316 bp
  • A to C, chromosome 12 at 4,315,819 bp
  • T to C, chromosome 13 at 98,260,791 bp
  • A to T, chromosome 13 at 100,535,029 bp
  • A to G, chromosome 13 at 100,612,470 bp
  • C to T, chromosome 14 at 66,309,262 bp
  • G to C, chromosome 15 at 58,314,978 bp
  • T to C, chromosome 15 at 66,683,898 bp
  • A to T, chromosome 15 at 100,313,168 bp
  • C to T, chromosome 16 at 5,245,308 bp
  • A to T, chromosome 16 at 17,844,977 bp
  • T to C, chromosome 16 at 37,062,097 bp
  • T to C, chromosome 16 at 90,224,342 bp
  • G to T, chromosome 17 at 5,433,470 bp
  • A to G, chromosome 17 at 34,039,677 bp
  • T to C, chromosome 17 at 34,600,703 bp
  • C to T, chromosome 17 at 46,240,896 bp
  • T to C, chromosome 17 at 47,687,294 bp
  • A to C, chromosome 17 at 56,125,600 bp
  • G to C, chromosome 17 at 57,255,135 bp
  • C to A, chromosome 17 at 73,494,266 bp
  • T to C, chromosome 18 at 36,287,750 bp
  • G to T, chromosome 18 at 84,209,793 bp
  • T to C, chromosome 19 at 10,571,603 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2259 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040259-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.