Strain Name:
C57BL/6J-MtgxR2277Btlr/Mmmh
Stock Number:
040276-MU
Citation ID:
RRID:MMRRC_040276-MU
Other Names:
R2277 (G1), C57BL/6J-MtgxR2277Btlr
Major Collection:

Strain Information

Itpk1
Name: inositol 1,3,4-triphosphate 5/6 kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217837
HGNC: HGNC:6177
Homologene: 8588
Lars
Name: leucyl-tRNA synthetase
Synonyms: 3110009L02Rik, 2310045K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 107045
VEGA: 18
HGNC: HGNC:6512
Homologene: 7083
Nphs1
Name: nephrosis 1, nephrin
Synonyms: nephrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 54631
HGNC: HGNC:7908
Homologene: 20974
Arhgap21
Name: Rho GTPase activating protein 21
Synonyms: 5530401C11Rik, ARHGAP10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71435
Homologene: 10822
Hdlbp
Name: high density lipoprotein (HDL) binding protein
Synonyms: D1Ertd101e, 1110005P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 110611
HGNC: HGNC:4857
Homologene: 38035
Ddx42
Name: DEAD box helicase 42
Synonyms: B430002H05Rik, 1810047H21Rik, SF3b125, DEAD (Asp-Glu-Ala-Asp) box polypeptide 42
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72047
Homologene: 49137
Sulf1
Name: sulfatase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240725
Homologene: 49408
Rbm28
Name: RNA binding motif protein 28
Synonyms: 2810480G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 68272
Homologene: 135952
Plcg1
Name: phospholipase C, gamma 1
Synonyms: Plc-1, Plcg-1, Plc-gamma1, Cded
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18803
HGNC: HGNC:9065
Homologene: 1997
Bdp1
Name: B double prime 1, subunit of RNA polymerase III transcription initiation factor IIIB
Synonyms: TFIIIB150, TFIIIB90, TAF3B1, TFC5, Tfnr, G630013P12Rik, B130055N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 544971
Homologene: 34582
Ankrd27
Name: ankyrin repeat domain 27 (VPS9 domain)
Synonyms: D330003H11Rik, Varp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 245886
Homologene: 12956
Top3a
Name: topoisomerase (DNA) III alpha
Synonyms: Top IIIa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 21975
Homologene: 3394
Ibtk
Name: inhibitor of Bruton agammaglobulinemia tyrosine kinase
Synonyms: 5430411K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 108837
Homologene: 34661
Serpina10
Name: serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
Synonyms: PZI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217847
Homologene: 9414
Ptpn4
Name: protein tyrosine phosphatase, non-receptor type 4
Synonyms: hPTP-MEG, protein tyrosine phosphatase, non-receptor type 4 (megakaryocyte), TEP/mPTPMEG, testis-enriched phosphatase, TEP, PTPMEG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19258
HGNC: HGNC:9656
Homologene: 2120
Rbp3
Name: retinol binding protein 3, interstitial
Synonyms: Irbp, Rbp-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 19661
VEGA: 14
HGNC: HGNC:9921
Homologene: 9261
Syne2
Name: spectrin repeat containing, nuclear envelope 2
Synonyms: nesprin-2, syne-2, D12Ertd777e, 6820443O06Rik, Nesp2g, Cpfl8, diminished cone electroretinogram, dice
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 319565
Homologene: 56700
Gh
Name: growth hormone
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14599
Homologene: 47932
Dnah17
Name: dynein, axonemal, heavy chain 17
Synonyms: LOC382552, 2810003K23Rik, Dnahcl1, Dnahc17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69926
HGNC: HGNC:2946
Homologene: 72102
Pcdhb10
Name: protocadherin beta 10
Synonyms: Pcdhb5D, PcdhbJ
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93881
HGNC: HGNC:8690
Homologene: 88834
Madd
Name: MAP-kinase activating death domain
Synonyms: 9630059K23Rik, Rab3 GEP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228355
HGNC: HGNC:6766
Homologene: 14249
Hcn3
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 3
Synonyms: Hac3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 15168
Homologene: 22453
Nlrp2
Name: NLR family, pyrin domain containing 2
Synonyms: PYPAF2, E330007A02Rik, Nalp2, Pan1, Nbs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232827
Homologene: 56789
Ttc23l
Name: tetratricopeptide repeat domain 23-like
Synonyms: 4930401A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 75777
Homologene: 44907
Lgi4
Name: leucine-rich repeat LGI family, member 4
Synonyms: clp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243914
Homologene: 16408
Cdh4
Name: cadherin 4
Synonyms: Rcad, R-cadherin, R-Cadh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12561
HGNC: HGNC:1763
Homologene: 48044
Slc25a29
Name: solute carrier family 25 (mitochondrial carrier, palmitoylcarnitine transporter), member 29
Synonyms: mCACL, CACL, C030003J19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 214663
Homologene: 5385
Atxn1
Name: ataxin 1
Synonyms: Atx1, Sca1, 2900016G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20238
VEGA: 13
Homologene: 281
Lrrcc1
Name: leucine rich repeat and coiled-coil domain containing 1
Synonyms: 4932441F23Rik, 1200008A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71710
Homologene: 12559
Ankrd23
Name: ankyrin repeat domain 23
Synonyms: MARP3, Darp, MARP3, 2310041C05Rik, 1110058D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 78321
Homologene: 14025
Slc23a2
Name: solute carrier family 23 (nucleobase transporters), member 2
Synonyms: YSPL3, SVCT2, Slc23a1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 54338
Homologene: 68440
Cr2
Name: complement receptor 2
Synonyms: CD21, CD35, Cr-1, Cr-2, Cr1, C3DR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12902
HGNC: HGNC:2336
Homologene: 55611
Hook2
Name: hook microtubule tethering protein 2
Synonyms: A630054I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 170833
Homologene: 8332
Pom121l2
Name: POM121 membrane glycoprotein-like 2 (rat)
Synonyms: LOC195236
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 195236
Homologene: 123536
Mpp2
Name: membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2)
Synonyms: Dlg2, Dlgh2, Pals4, D11Bwg0652e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 50997
HGNC: HGNC:7220
Homologene: 3920
Cela3a
Name: chymotrypsin-like elastase family, member 3A
Synonyms: Gm13011
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242711
Homologene: 129880
Mrgprb5
Name: MAS-related GPR, member B5
Synonyms: MrgB5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 404239
Homologene: 115575
Rhebl1
Name: Ras homolog enriched in brain like 1
Synonyms: 1810036J22Rik, Rheb2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 69159
Homologene: 5477
Ankrd13d
Name: ankyrin repeat domain 13 family, member D
Synonyms: 0710001P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 68423
Homologene: 27612
Gm6994
Name: predicted gene 6994
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 629678
Dnajc28
Name: DnaJ heat shock protein family (Hsp40) member C28
Synonyms: ORF28
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 246738
HGNC: HGNC:1297
Homologene: 9869
Tmem45a
Name: transmembrane protein 45a
Synonyms: C630002M10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 56277
Homologene: 41215
Runx1t1
Name: RUNX1 translocation partner 1
Synonyms: ETO, MTG8, Cbfa2t1h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12395
HGNC: HGNC:1535
Homologene: 3801
Mamld1
Name: mastermind-like domain containing 1
Synonyms: G630014P10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 333639
HGNC: HGNC:2568
Homologene: 135712
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 12,796,794 bp
  • A to C, chromosome 1 at 36,534,442 bp
  • G to A, chromosome 1 at 93,408,178 bp
  • T to C, chromosome 1 at 119,684,591 bp
  • T to C, chromosome 1 at 195,157,368 bp
  • T to C, chromosome 2 at 20,863,226 bp
  • A to T, chromosome 2 at 91,143,683 bp
  • A to G, chromosome 2 at 132,091,259 bp
  • T to A, chromosome 2 at 160,755,805 bp
  • C to A, chromosome 2 at 179,797,524 bp
  • T to C, chromosome 3 at 14,554,292 bp
  • C to A, chromosome 3 at 89,147,861 bp
  • T to C, chromosome 4 at 13,771,501 bp
  • T to C, chromosome 4 at 137,405,876 bp
  • T to C, chromosome 6 at 29,135,514 bp
  • T to C, chromosome 7 at 5,328,129 bp
  • T to A, chromosome 7 at 30,467,564 bp
  • T to G, chromosome 7 at 31,060,612 bp
  • T to C, chromosome 7 at 35,615,840 bp
  • A to G, chromosome 7 at 48,168,831 bp
  • C to T, chromosome 8 at 85,002,931 bp
  • T to C, chromosome 9 at 85,703,151 bp
  • A to C, chromosome 11 at 60,745,874 bp
  • T to C, chromosome 11 at 102,064,301 bp
  • T to A, chromosome 11 at 106,242,939 bp
  • T to C, chromosome 11 at 106,300,787 bp
  • T to C, chromosome 11 at 118,096,561 bp
  • A to G, chromosome 12 at 75,927,466 bp
  • T to C, chromosome 12 at 102,570,260 bp
  • T to A, chromosome 12 at 103,626,743 bp
  • T to C, chromosome 12 at 108,826,926 bp
  • T to C, chromosome 13 at 21,984,247 bp
  • T to C, chromosome 13 at 45,557,068 bp
  • C to A, chromosome 13 at 100,061,330 bp
  • T to A, chromosome 13 at 100,061,339 bp
  • C to T, chromosome 14 at 33,956,018 bp
  • A to G, chromosome 14 at 77,485,995 bp
  • A to G, chromosome 15 at 10,523,592 bp
  • A to T, chromosome 15 at 98,878,286 bp
  • A to T, chromosome 16 at 56,823,519 bp
  • G to A, chromosome 16 at 91,616,867 bp
  • T to C, chromosome 18 at 37,412,624 bp
  • C to T, chromosome 18 at 42,235,502 bp
  • T to C, chromosome 19 at 4,280,984 bp
  • ACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCA to ACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCA, chromosome X at 71,118,815 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2277 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040276-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.