Strain Name:
C57BL/6J-MtgxR2324Btlr/Mmmh
Stock Number:
040315-MU
Citation ID:
RRID:MMRRC_040315-MU
Other Names:
R2324 (G1), C57BL/6J-MtgxR2324Btlr
Major Collection:

Gene Information

Vangl2
Name: VANGL planar cell polarity 2
Synonyms: Lpp1, C530001F03Rik, skam17Jus, Ltap, strabismus, loop-tail, ska17, Lootl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 93840
Homologene: 62161
Npm1
Name: nucleophosmin 1
Synonyms: nucleolar protein NO38, B23, NO38
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18148
HGNC: HGNC:7910
Homologene: 81697
Notch3
Name: notch 3
Synonyms: hpbk, N3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18131
HGNC: HGNC:7883
Homologene: 376
Lactb2
Name: lactamase, beta 2
Synonyms: E430032H21Rik, Cgi-83
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 212442
Homologene: 9349
Ints3
Name: integrator complex subunit 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229543
Homologene: 11309
Pabpc4
Name: poly(A) binding protein, cytoplasmic 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230721
HGNC: HGNC:8557
Homologene: 37855
Esyt2
Name: extended synaptotagmin-like protein 2
Synonyms: 2410017M09Rik, 4921504I16Rik, 2310058N22Rik, D12Ertd551e, Fam62b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 52635
VEGA: 12
Homologene: 32699
Ints12
Name: integrator complex subunit 12
Synonyms: A230056J18Rik, 4930529N21Rik, 2810027J24Rik, 1110020M19Rik, Phf22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71793
Homologene: 32474
Slu7
Name: SLU7 splicing factor homolog (S. cerevisiae)
Synonyms: D3Bwg0878e, D11Ertd730e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 193116
Homologene: 4690
Mib2
Name: mindbomb E3 ubiquitin protein ligase 2
Synonyms: 2210008I11Rik, Zzank1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 76580
Homologene: 16062
Cep152
Name: centrosomal protein 152
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99100
Homologene: 37159
Ttc13
Name: tetratricopeptide repeat domain 13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234875
Homologene: 11569
Dpy19l4
Name: dpy-19-like 4 (C. elegans)
Synonyms: LOC381510, Narg3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381510
Homologene: 18773
Hectd4
Name: HECT domain E3 ubiquitin protein ligase 4
Synonyms: Gm15800
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269700
Homologene: 28297
Zfp988
Name: zinc finger protein 988
Synonyms: Gm13151
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 77505
Homologene: 131117
Lrp1
Name: low density lipoprotein receptor-related protein 1
Synonyms: CD91, A2mr, b2b1554Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16971
HGNC: HGNC:6692
Homologene: 1744
Clca3a2
Name: chloride channel accessory 3A2
Synonyms: Clca2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 80797
Homologene: 77224
Akna
Name: AT-hook transcription factor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100182
Homologene: 49947
Skint6
Name: selection and upkeep of intraepithelial T cells 6
Synonyms: OTTMUSG00000008519
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230622
Homologene: 135888
Nfic
Name: nuclear factor I/C
Synonyms: NF1-C, 1500041O16Rik, 1110019L22Rik, nuclear factor 1-C2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18029
HGNC: HGNC:7786
Homologene: 4088
Gm281
Name: predicted gene 281
Synonyms: LOC238939
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 238939
Homologene: 141164
Lmntd2
Name: lamin tail domain containing 2
Synonyms: 1600016N20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72000
Homologene: 104159
Ankrd42
Name: ankyrin repeat domain 42
Synonyms: 4933417L02Rik, Ikbn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73845
Homologene: 23652
Oas2
Name: 2'-5' oligoadenylate synthetase 2
Synonyms: 2'-5' oligoadenylate synthetase-like 11, Oasl11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 246728
HGNC: HGNC:8087
Homologene: 49478
Rbsn
Name: rabenosyn, RAB effector
Synonyms: 5330426D11Rik, Rabenosyn-5, Zfyve20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 78287
Homologene: 41477
Irf9
Name: interferon regulatory factor 9
Synonyms: p48, Irf-9, Isgf3g
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 16391
HGNC: HGNC:6131
Homologene: 4436
Vax2
Name: ventral anterior homeobox 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 24113
Homologene: 8048
Coa8
Name: cytochrome c oxidase assembly factor 8
Synonyms: 1700081D05Rik, Apop-1, 2810002N01Rik, Apopt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 68020
Homologene: 12225
Piwil4
Name: piwi-like RNA-mediated gene silencing 4
Synonyms: 9230101H05Rik, Miwi2, MIWI2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 330890
Homologene: 45122
Lrrc56
Name: leucine rich repeat containing 56
Synonyms: 5730427C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 70552
Homologene: 115748
Tmem181a
Name: transmembrane protein 181A
Synonyms: 5930418K15Rik, Gpr178, C76977, Tmem181
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 77106
Homologene: 44787
Olfr178
Name: olfactory receptor 178
Synonyms: GA_x54KRFPKG5P-55108059-55107100, MOR184-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258999
Homologene: 79412
Efcab8
Name: EF-hand calcium binding domain 8
Synonyms: EG329541
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 100504221
Tmem165
Name: transmembrane protein 165
Synonyms: pFT27, Tpardl, Tparl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21982
Homologene: 5411
Rhox2e
Name: reproductive homeobox 2E
Synonyms: ENSMUSG00000063709, OTTMUSG00000017141
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 100040016
Homologene: 41772
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 13,638,296 bp
  • G to T, chromosome 1 at 172,006,333 bp
  • T to C, chromosome 2 at 125,563,462 bp
  • A to G, chromosome 2 at 153,783,809 bp
  • A to G, chromosome 3 at 90,394,094 bp
  • G to A, chromosome 3 at 133,109,365 bp
  • A to G, chromosome 3 at 144,806,280 bp
  • T to C, chromosome 4 at 11,276,857 bp
  • T to C, chromosome 4 at 63,371,802 bp
  • T to C, chromosome 4 at 112,872,457 bp
  • T to C, chromosome 4 at 123,297,778 bp
  • A to C, chromosome 4 at 147,332,785 bp
  • C to T, chromosome 4 at 155,655,243 bp
  • T to C, chromosome 5 at 76,204,824 bp
  • T to C, chromosome 5 at 120,743,274 bp
  • G to A, chromosome 5 at 121,315,437 bp
  • G to T, chromosome 6 at 83,711,325 bp
  • A to G, chromosome 6 at 92,193,966 bp
  • C to A, chromosome 7 at 92,623,978 bp
  • A to T, chromosome 7 at 105,710,090 bp
  • A to G, chromosome 7 at 141,205,563 bp
  • A to T, chromosome 7 at 141,210,788 bp
  • A to G, chromosome 8 at 124,679,057 bp
  • C to T, chromosome 9 at 14,736,908 bp
  • A to G, chromosome 10 at 81,406,087 bp
  • C to A, chromosome 10 at 127,566,586 bp
  • A to G, chromosome 11 at 33,155,439 bp
  • T to C, chromosome 11 at 43,438,793 bp
  • A to G, chromosome 12 at 111,724,350 bp
  • A to G, chromosome 12 at 116,367,821 bp
  • A to T, chromosome 14 at 13,868,077 bp
  • T to C, chromosome 14 at 55,605,910 bp
  • T to C, chromosome 16 at 58,890,140 bp
  • T to A, chromosome 17 at 6,295,786 bp
  • G to A, chromosome 17 at 32,150,134 bp
  • C to A, chromosome X at 37,530,863 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2324 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040315-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.