Strain Name:
C57BL/6J-MtgxR2422Btlr/Mmmh
Stock Number:
040384-MU
Citation ID:
RRID:MMRRC_040384-MU
Other Names:
R2422 (G1), C57BL/6J-MtgxR2422Btlr
Major Collection:

Gene Information

Gene Symbol: Rgs9 [MGI:1338824] (Mus musculus (mouse))
Name: regulator of G-protein signaling 9
Synonyms: RGS9-1, Rgs9-2
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Glrb [MGI:95751] (Mus musculus (mouse))
Name: glycine receptor, beta subunit
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Adck2 [MGI:1889336] (Mus musculus (mouse))
Name: aarF domain containing kinase 2
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Dnajc21 [MGI:1925371] (Mus musculus (mouse))
Name: DnaJ heat shock protein family (Hsp40) member C21
Synonyms: 9930116P15Rik, 4930461P20Rik
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Spred1 [MGI:2150016] (Mus musculus (mouse))
Name: sprouty protein with EVH-1 domain 1, related sequence
Synonyms: Spred-1, 5730461F13Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Kcnc4 [MGI:96670] (Mus musculus (mouse))
Name: potassium voltage gated channel, Shaw-related subfamily, member 4
Synonyms: Kv3.4, Kcr2-4
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Mib1 [MGI:2443157] (Mus musculus (mouse))
Name: mindbomb E3 ubiquitin protein ligase 1
Synonyms: Mib, mind bomb-1, skeletrophin, E430019M12Rik
Chromosome: 18
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 32,545,861 bp
  • C to A, chromosome 1 at 37,613,475 bp
  • C to A, chromosome 1 at 45,384,388 bp
  • G to A, chromosome 2 at 6,553,889 bp
  • A to T, chromosome 2 at 68,591,520 bp
  • T to C, chromosome 2 at 70,770,005 bp
  • C to T, chromosome 2 at 104,471,485 bp
  • T to C, chromosome 2 at 117,165,010 bp
  • T to C, chromosome 2 at 119,655,737 bp
  • C to T, chromosome 2 at 120,700,284 bp
  • T to C, chromosome 2 at 158,636,081 bp
  • C to T, chromosome 2 at 167,484,206 bp
  • C to A, chromosome 3 at 5,390,405 bp
  • A to G, chromosome 3 at 80,860,235 bp
  • T to A, chromosome 3 at 100,027,619 bp
  • A to T, chromosome 3 at 105,960,021 bp
  • G to T, chromosome 3 at 107,445,547 bp
  • A to T, chromosome 3 at 108,095,539 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • A to G, chromosome 4 at 99,748,782 bp
  • G to T, chromosome 4 at 106,561,094 bp
  • T to C, chromosome 5 at 32,991,035 bp
  • A to G, chromosome 5 at 86,132,616 bp
  • A to G, chromosome 5 at 89,683,175 bp
  • G to A, chromosome 5 at 104,815,277 bp
  • A to G, chromosome 5 at 106,637,244 bp
  • A to C, chromosome 5 at 108,228,588 bp
  • A to G, chromosome 5 at 109,086,532 bp
  • A to T, chromosome 5 at 112,355,118 bp
  • C to T, chromosome 6 at 39,583,998 bp
  • C to T, chromosome 6 at 83,199,800 bp
  • C to A, chromosome 6 at 83,966,439 bp
  • A to G, chromosome 6 at 120,339,159 bp
  • A to T, chromosome 6 at 132,855,594 bp
  • T to A, chromosome 7 at 10,362,945 bp
  • T to C, chromosome 8 at 61,019,901 bp
  • A to T, chromosome 8 at 71,321,855 bp
  • C to A, chromosome 8 at 110,222,781 bp
  • C to A, chromosome 9 at 20,499,262 bp
  • A to G, chromosome 9 at 39,950,528 bp
  • C to A, chromosome 9 at 56,918,538 bp
  • T to C, chromosome 9 at 73,931,547 bp
  • T to C, chromosome 9 at 103,365,118 bp
  • G to A, chromosome 9 at 109,108,438 bp
  • G to A, chromosome 10 at 3,958,048 bp
  • T to A, chromosome 11 at 69,804,537 bp
  • T to G, chromosome 11 at 98,219,074 bp
  • C to A, chromosome 11 at 109,225,777 bp
  • A to T, chromosome 12 at 8,748,931 bp
  • A to G, chromosome 12 at 101,042,653 bp
  • C to A, chromosome 12 at 112,610,824 bp
  • T to A, chromosome 12 at 113,369,487 bp
  • T to C, chromosome 13 at 62,871,306 bp
  • T to G, chromosome 14 at 16,409,189 bp
  • T to C, chromosome 14 at 50,453,436 bp
  • C to A, chromosome 14 at 54,857,574 bp
  • A to G, chromosome 14 at 121,954,177 bp
  • C to T, chromosome 15 at 9,194,765 bp
  • A to G, chromosome 15 at 10,461,935 bp
  • CT to CTTGGATT, chromosome 15 at 10,537,562 bp
  • G to A, chromosome 15 at 10,537,566 bp
  • T to C, chromosome 15 at 55,449,922 bp
  • C to T, chromosome 15 at 66,678,020 bp
  • T to C, chromosome 15 at 98,862,266 bp
  • T to C, chromosome 15 at 101,947,264 bp
  • T to C, chromosome 16 at 8,487,361 bp
  • T to C, chromosome 16 at 58,889,965 bp
  • T to A, chromosome 17 at 20,227,835 bp
  • G to A, chromosome 17 at 35,078,146 bp
  • A to G, chromosome 17 at 36,774,999 bp
  • A to T, chromosome 17 at 67,750,553 bp
  • T to A, chromosome 17 at 74,660,614 bp
  • T to C, chromosome 18 at 10,751,906 bp
  • T to A, chromosome 18 at 51,865,784 bp
  • A to T, chromosome 18 at 82,405,923 bp
  • A to T, chromosome 19 at 5,652,112 bp
  • A to T, chromosome 19 at 12,268,919 bp
  • T to C, chromosome 19 at 38,124,344 bp
  • A to T, chromosome 19 at 43,728,088 bp
  • A to G, chromosome X at 152,047,975 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R2422 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    040384-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.