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Strain Name:
C57BL/6J-MtgxR2517Btlr/Mmmh
Stock Number:
040421-MU
Citation ID:
RRID:MMRRC_040421-MU
Other Names:
R2517 (G1), C57BL/6J-MtgxR2517Btlr
Major Collection:

Strain Information

Glrb
Name: glycine receptor, beta subunit
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14658
HGNC: HGNC:4329
Homologene: 20224
Ssu72
Name: Ssu72 RNA polymerase II CTD phosphatase homolog (yeast)
Synonyms: 2610101M12Rik, 1190002E22Rik, 1500011L16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68991
Homologene: 6754
Gnl3
Name: guanine nucleotide binding protein nucleolar 3
Synonyms: nucleostemin, NS
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 30877
VEGA: 14
Homologene: 56670
Dip2c
Name: disco interacting protein 2 homolog C
Synonyms: 2900024P20Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 208440
Homologene: 40996
Gfpt2
Name: glutamine fructose-6-phosphate transaminase 2
Synonyms: GFAT2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14584
HGNC: HGNC:4242
Homologene: 68439
Enpp2
Name: ectonucleotide pyrophosphatase/phosphodiesterase 2
Synonyms: Autotaxin, Npps2, PD-Ialpha, Pdnp2, ATX
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 18606
HGNC: HGNC:3357
Homologene: 4526
Atp2a2
Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Synonyms: sarco/endoplasmic reticulum Ca2+-ATPase 2, SERCA2, 9530097L16Rik, D5Wsu150e, SERCA2B, Serca2a
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11938
HGNC: HGNC:812
Homologene: 80167
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 82,680,710 bp
  • A to G, chromosome 1 at 132,597,763 bp
  • C to T, chromosome 2 at 36,189,097 bp
  • A to G, chromosome 2 at 57,999,413 bp
  • C to T, chromosome 2 at 66,273,832 bp
  • A to T, chromosome 2 at 69,936,018 bp
  • A to G, chromosome 2 at 132,556,981 bp
  • T to C, chromosome 2 at 181,259,026 bp
  • A to T, chromosome 3 at 75,892,859 bp
  • A to T, chromosome 3 at 80,861,747 bp
  • A to C, chromosome 3 at 94,531,234 bp
  • T to C, chromosome 4 at 47,208,492 bp
  • A to T, chromosome 4 at 100,980,882 bp
  • A to G, chromosome 4 at 116,173,594 bp
  • G to A, chromosome 4 at 126,439,939 bp
  • C to T, chromosome 4 at 141,464,585 bp
  • T to C, chromosome 4 at 155,733,513 bp
  • G to A, chromosome 5 at 38,126,999 bp
  • A to T, chromosome 5 at 73,421,543 bp
  • A to G, chromosome 5 at 115,445,458 bp
  • G to A, chromosome 5 at 122,457,513 bp
  • A to G, chromosome 5 at 150,539,672 bp
  • T to A, chromosome 6 at 83,147,545 bp
  • C to A, chromosome 6 at 85,667,963 bp
  • A to G, chromosome 6 at 123,329,978 bp
  • A to T, chromosome 6 at 129,639,557 bp
  • A to C, chromosome 6 at 146,992,444 bp
  • G to A, chromosome 7 at 20,049,410 bp
  • T to C, chromosome 7 at 27,663,263 bp
  • A to T, chromosome 7 at 45,634,823 bp
  • A to G, chromosome 7 at 144,052,305 bp
  • C to T, chromosome 8 at 83,572,575 bp
  • A to T, chromosome 9 at 19,249,061 bp
  • A to T, chromosome 9 at 46,288,148 bp
  • A to T, chromosome 9 at 63,512,223 bp
  • G to A, chromosome 9 at 114,386,196 bp
  • C to A, chromosome 10 at 14,128,969 bp
  • T to A, chromosome 11 at 3,221,168 bp
  • T to C, chromosome 11 at 49,809,029 bp
  • T to A, chromosome 11 at 51,048,473 bp
  • T to C, chromosome 11 at 60,468,128 bp
  • T to G, chromosome 11 at 61,141,476 bp
  • C to T, chromosome 11 at 69,516,644 bp
  • T to C, chromosome 11 at 90,671,125 bp
  • T to C, chromosome 12 at 31,075,239 bp
  • A to G, chromosome 12 at 76,649,869 bp
  • A to G, chromosome 13 at 9,609,005 bp
  • C to A, chromosome 13 at 55,542,816 bp
  • T to A, chromosome 13 at 120,263,939 bp
  • A to G, chromosome 14 at 31,014,163 bp
  • T to A, chromosome 14 at 32,863,707 bp
  • C to A, chromosome 14 at 55,781,946 bp
  • A to T, chromosome 14 at 55,788,035 bp
  • T to C, chromosome 14 at 75,741,742 bp
  • T to C, chromosome 14 at 79,214,054 bp
  • T to C, chromosome 15 at 9,335,795 bp
  • A to G, chromosome 15 at 9,725,197 bp
  • A to C, chromosome 15 at 38,297,113 bp
  • A to T, chromosome 15 at 54,919,694 bp
  • T to A, chromosome 15 at 73,124,242 bp
  • A to T, chromosome 15 at 77,517,195 bp
  • C to A, chromosome 15 at 82,043,981 bp
  • T to C, chromosome 15 at 84,328,759 bp
  • A to T, chromosome 15 at 102,453,175 bp
  • A to T, chromosome 16 at 22,988,315 bp
  • A to G, chromosome 16 at 29,297,397 bp
  • G to A, chromosome 16 at 37,089,325 bp
  • A to G, chromosome 17 at 20,472,315 bp
  • G to A, chromosome 17 at 47,703,072 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2517 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040421-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.