Beutler Mutagenetix

Strain Name:
C57BL/6J-MtgxR2862Btlr/Mmmh
Stock Number:
040452-MU
Citation ID:
RRID:MMRRC_040452-MU
Other Names:
R2862 (G1), C57BL/6J-MtgxR2862Btlr
Major Collection:

Gene Information

Mrps18b
Name: mitochondrial ribosomal protein S18B
Synonyms: 2400002C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 66973
Homologene: 32205
Ctage5
Name: CTAGE family, member 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
Cdca2
Name: cell division cycle associated 2
Synonyms: 2610311M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 108912
Homologene: 18444
Ppp6r3
Name: protein phosphatase 6, regulatory subunit 3
Synonyms: Saps3, 9130026N02Rik, D19Bwg1430e, 4930528G08Rik, Pp6r3, Pptcs3, D19Ertd703e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 52036
VEGA: 19
HGNC: HGNC:1173
Homologene: 115911
Tbc1d8
Name: TBC1 domain family, member 8
Synonyms: AD3, GRAM domain, BUB2-like protein 1, HBLP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 54610
Homologene: 31421
Cap1
Name: CAP, adenylate cyclase-associated protein 1 (yeast)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 12331
Homologene: 74572
Aqr
Name: aquarius
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 11834
Homologene: 7629
Ahi1
Name: Abelson helper integration site 1
Synonyms: 1700015F03Rik, Ahi-1, Jouberin, D10Bwg0629e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 52906
VEGA: 10
Homologene: 9762
Apoe
Name: apolipoprotein E
Synonyms: Apoe
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 11816
HGNC: HGNC:613
Homologene: 30951
Lamp5
Name: lysosomal-associated membrane protein family, member 5
Synonyms: 3110035N03Rik, BAD-LAMP, 6330527O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 76161
Homologene: 8173
Nmnat2
Name: nicotinamide nucleotide adenylyltransferase 2
Synonyms: PNAT1, D030041I09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 226518
Homologene: 75037
Ntn1
Name: netrin 1
Synonyms: Netrin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 18208
HGNC: HGNC:8029
Homologene: 21008
Ube2v1
Name: ubiquitin-conjugating enzyme E2 variant 1
Synonyms: D7Bwg1382e, CROC1, 0610011J09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 66589
Homologene: 81888
Itgb3bp
Name: integrin beta 3 binding protein (beta3-endonexin)
Synonyms: 4930471O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 67733
HGNC: HGNC:6157
Homologene: 8617
Rreb1
Name: ras responsive element binding protein 1
Synonyms: 1110037N09Rik, B930013M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 68750
Homologene: 2218
Rnf41
Name: ring finger protein 41
Synonyms: 4930511A05Rik, 4933415P08Rik, Nrdp1, D10Ertd722e, FLRF, 2210404G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 67588
VEGA: 10
Homologene: 4226
Tmem131l
Name: transmembrane 131 like
Synonyms: D930015E06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229473
Homologene: 9057
Opn4
Name: opsin 4 (melanopsin)
Synonyms: 1110007J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 30044
VEGA: 14
Homologene: 69152
Btg3
Name: B cell translocation gene 3
Synonyms: ANA, tob5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 12228
HGNC: HGNC:1132
Homologene: 4953
Dnah1
Name: dynein, axonemal, heavy chain 1
Synonyms: B230373P09Rik, E030034C22Rik, Dnahc1, MDHC7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 110084
VEGA: 14
HGNC: HGNC:2940
Homologene: 67131
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 77505
Homologene: 131117
Pex2
Name: peroxisomal biogenesis factor 2
Synonyms: D3Ertd138e, Pxmp3, PMP35, Zellweger syndrome homolog
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 19302
HGNC: HGNC:9717
Homologene: 269
Col1a2
Name: collagen, type I, alpha 2
Synonyms: Col1a-2, Cola-2, Cola2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 12843
HGNC: HGNC:2198
Homologene: 69
Abca13
Name: ATP-binding cassette, sub-family A (ABC1), member 13
Synonyms: A930002G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 268379
Homologene: 27991
Lama2
Name: laminin, alpha 2
Synonyms: mer, merosin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 16773
HGNC: HGNC:6482
Homologene: 37306
Snx13
Name: sorting nexin 13
Synonyms: RGS-PX1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 217463
VEGA: 12
Homologene: 41011
F5
Name: coagulation factor V
Synonyms: Cf5, Cf-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 14067
HGNC: HGNC:3542
Homologene: 104
Mrgbp
Name: MRG/MORF4L binding protein
Synonyms: 1600027N09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 73247
Homologene: 10104
Srgap3
Name: SLIT-ROBO Rho GTPase activating protein 3
Synonyms: Arhgap14, D130026O08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 259302
Homologene: 56686
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: PKHDL1, fibrocystin L, D86 mRNA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 192190
Homologene: 16332
Lama3
Name: laminin, alpha 3
Synonyms: nicein, 150kDa, [a]3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 16774
HGNC: HGNC:6483
Homologene: 18279
Olfr1214
Name: olfactory receptor 1214
Synonyms: MOR233-8, GA_x6K02T2Q125-50468705-50467770
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258899
HGNC: null
Homologene: 73984
Capn2
Name: calpain 2
Synonyms: m-calpain, Capa-2, Capa2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12334
HGNC: HGNC:1479
Homologene: 1326
Vmn2r72
Name: vomeronasal 2, receptor 72
Synonyms: Vmn2r72-ps, EG244114
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 244114
HGNC: null
Homologene: 115466
Greb1
Name: gene regulated by estrogen in breast cancer protein
Synonyms: 5730583K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 268527
Homologene: 8780
Col22a1
Name: collagen, type XXII, alpha 1
Synonyms: C80743, 2310067L16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 69700
Homologene: 43567
Mapk12
Name: mitogen-activated protein kinase 12
Synonyms: P38gamma, Prkm12, Erk6, Sapk3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 29857
VEGA: 15
HGNC: HGNC:6874
Homologene: 55705
Rxfp1
Name: relaxin/insulin-like family peptide receptor 1
Synonyms: Lgr7, LOC381489
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 381489
Homologene: 11007
Pate3
Name: prostate and testis expressed 3
Synonyms: Gm17365
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 100312956
Homologene: 129908
Synj1
Name: synaptojanin 1
Synonyms: A930006D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 104015
Homologene: 48252
Olfr231
Name: olfactory receptor 231
Synonyms: GA_x6K02T2LER7-6-623, GA_x6K02T02K39-452-3, GA_x6K02T2P20D-21053942-21054499, MOR105-4, Olfr244, Olfr425
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 404222
Homologene: 17363
Med14
Name: mediator complex subunit 14
Synonyms: LOC270579, ORF1, 9930001L01Rik, Trap170, ENSMUSG00000073278, Crsp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 26896
HGNC: HGNC:2370
Homologene: 22082
Zc3h6
Name: zinc finger CCCH type containing 6
Synonyms: 4833425H18Rik, 4631426G04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 78751
Homologene: 35328
Cyp2c38
Name: cytochrome P450, family 2, subfamily c, polypeptide 38
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 13097
HGNC: HGNC:2622
Homologene: 117948
Smyd4
Name: SET and MYND domain containing 4
Synonyms: G430029E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 319822
Homologene: 35098
Il18r1
Name: interleukin 18 receptor 1
Synonyms: Il1rrp, Il18ralpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 16182
HGNC: HGNC:5988
Homologene: 2861
Ears2
Name: glutamyl-tRNA synthetase 2, mitochondrial
Synonyms: 3230401I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 67417
Homologene: 6907
Gata5
Name: GATA binding protein 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 14464
Homologene: 32031
4930548H24Rik
Name: RIKEN cDNA 4930548H24 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 67656
Homologene: null
Abcd1
Name: ATP-binding cassette, sub-family D (ALD), member 1
Synonyms: ALDP, Aldgh, Ald
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 11666
HGNC: HGNC:61
Homologene: 55426
Actg1
Name: actin, gamma, cytoplasmic 1
Synonyms: E51, Actl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 11465
HGNC: HGNC:144
Homologene: 74402
Tinf2
Name: Terf1 (TRF1)-interacting nuclear factor 2
Synonyms: TIN2, D14Wsu146e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 28113
VEGA: 14
Homologene: 8264
Pwwp3b
Name: PWWP domain containing 3B
Synonyms: Mum1l1, 9430086K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 245631
Homologene: 51851
CAAA01083517.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
Grap2
Name: GRB2-related adaptor protein 2
Synonyms: Grf40, Mona, P38, GRB2L, Grb2-related adaptor downstream of Sch, GrpL, Gads, GRID, GrbX, GRAP-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 17444
VEGA: 15
HGNC: HGNC:4563
Homologene: 21007
Rnf113a1
Name: ring finger protein 113A1
Synonyms: 2810428C21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 69942
Homologene: 133822
CAAA01066805.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
Ang
Name: angiogenin, ribonuclease, RNase A family, 5
Synonyms: Rnase5a, Ang1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 11727
VEGA: 14
HGNC: HGNC:483
Homologene: 74385
Olfr715b
Name: olfactory receptor 715B
Synonyms: EG384732, Gm10081
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 384732
HGNC: HGNC:8244
Homologene: 81541
Gm11938
Name: predicted gene 11938
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 100041412
Homologene: 41961
Gm4862
Name: predicted gene 4862
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229879
HGNC: null
Homologene: null
Maged1
Name: melanoma antigen, family D, 1
Synonyms: MAGE-D1, Dlxin-1, Nrage, 5430405L04Rik, DXBwg1492e, 2810433C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 94275
HGNC: HGNC:6813
Homologene: 5077
1700021P04Rik
Name: RIKEN cDNA 1700021P04 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: null
HGNC: null
Homologene: null
Slc35e4
Name: solute carrier family 35, member E4
Synonyms: A330108F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 103710
Homologene: 37409
Kdf1
Name: keratinocyte differentiation factor 1
Synonyms: 1810019J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 69073
Homologene: 17563
Vegfd
Name: vascular endothelial growth factor D
Synonyms: Figf, VEGF-D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 14205
HGNC: HGNC:3708
Homologene: 3288
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 39,402,696 bp
  • T to C, chromosome 1 at 40,498,557 bp
  • G to A, chromosome 1 at 153,112,425 bp
  • A to T, chromosome 1 at 164,184,964 bp
  • A to G, chromosome 1 at 174,117,732 bp
  • A to T, chromosome 1 at 182,472,920 bp
  • A to G, chromosome 1 at 183,356,434 bp
  • A to T, chromosome 2 at 88,987,320 bp
  • A to T, chromosome 2 at 114,136,917 bp
  • A to G, chromosome 2 at 129,015,460 bp
  • A to T, chromosome 2 at 136,058,946 bp
  • G to A, chromosome 2 at 167,617,965 bp
  • C to T, chromosome 2 at 180,334,336 bp
  • G to A, chromosome 2 at 180,583,410 bp
  • A to G, chromosome 3 at 5,561,180 bp
  • A to G, chromosome 3 at 79,682,471 bp
  • A to T, chromosome 3 at 83,923,109 bp
  • A to G, chromosome 3 at 139,128,477 bp
  • T to C, chromosome 4 at 99,802,088 bp
  • A to T, chromosome 4 at 122,864,725 bp
  • G to A, chromosome 4 at 133,528,541 bp
  • A to G, chromosome 4 at 156,237,450 bp
  • G to A, chromosome 5 at 31,485,911 bp
  • G to A, chromosome 6 at 4,518,822 bp
  • A to T, chromosome 6 at 112,722,972 bp
  • T to C, chromosome 7 at 19,697,554 bp
  • A to T, chromosome 7 at 85,750,836 bp
  • A to G, chromosome 7 at 105,712,559 bp
  • T to A, chromosome 7 at 107,106,468 bp
  • A to T, chromosome 7 at 122,062,940 bp
  • T to A, chromosome 9 at 35,648,119 bp
  • T to A, chromosome 10 at 20,981,408 bp
  • G to A, chromosome 10 at 27,422,612 bp
  • T to C, chromosome 10 at 128,438,154 bp
  • A to T, chromosome 11 at 3,912,796 bp
  • A to G, chromosome 11 at 9,309,057 bp
  • T to C, chromosome 11 at 68,385,864 bp
  • T to A, chromosome 11 at 75,390,136 bp
  • C to A, chromosome 11 at 99,603,146 bp
  • T to C, chromosome 11 at 120,346,801 bp
  • A to G, chromosome 12 at 16,711,745 bp
  • T to A, chromosome 12 at 35,138,117 bp
  • A to G, chromosome 12 at 59,154,410 bp
  • G to C, chromosome 13 at 37,932,453 bp
  • C to T, chromosome 14 at 31,284,762 bp
  • A to G, chromosome 14 at 34,593,828 bp
  • G to T, chromosome 14 at 51,101,818 bp
  • T to C, chromosome 14 at 55,680,631 bp
  • T to C, chromosome 14 at 67,698,090 bp
  • A to C, chromosome 15 at 44,540,871 bp
  • A to G, chromosome 15 at 71,815,943 bp
  • A to T, chromosome 15 at 80,647,964 bp
  • T to C, chromosome 15 at 89,137,472 bp
  • A to G, chromosome 16 at 78,364,980 bp
  • T to C, chromosome 16 at 90,969,329 bp
  • G to A, chromosome 17 at 35,910,854 bp
  • G to T, chromosome 18 at 12,453,750 bp
  • A to C, chromosome 19 at 3,521,782 bp
  • C to T, chromosome 19 at 24,066,231 bp
  • G to A, chromosome 19 at 39,460,694 bp
  • A to G, chromosome X at 12,719,697 bp
  • A to G, chromosome X at 37,192,083 bp
  • T to A, chromosome X at 73,737,458 bp
  • G to A, chromosome X at 94,538,924 bp
  • G to A, chromosome X at 139,236,680 bp
  • A to G, chromosome X at 164,385,883 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2862 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040452-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.