Strain Name:
C57BL/6J-MtgxR2863Btlr/Mmmh
Stock Number:
040453-MU
Citation ID:
RRID:MMRRC_040453-MU
Other Names:
R2863 (G1), C57BL/6J-MtgxR2863Btlr
Major Collection:

Gene Information

Ldb2
Name: LIM domain binding 2
Synonyms: CLIM1, Ldb3, CLP-36, CLIM-1b, CLIM-1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 16826
HGNC: HGNC:6533
Homologene: 989
Ppp4c
Name: protein phosphatase 4, catalytic subunit
Synonyms: PPX, 1110002D08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56420
HGNC: HGNC:9319
Homologene: 2038
Armh3
Name: armadillo-like helical domain containing 3
Synonyms: 9130011E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 71617
VEGA: 19
Homologene: 15843
Exoc6
Name: exocyst complex component 6
Synonyms: msec15, 4833405E05Rik, Sec15, Sec15l1, hbd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107371
VEGA: 19
Homologene: 41305
Fxr2
Name: fragile X mental retardation, autosomal homolog 2
Synonyms: Fxr2h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 23879
HGNC: HGNC:4024
Homologene: 21014
Epb41l3
Name: erythrocyte membrane protein band 4.1 like 3
Synonyms: NBL3, 4.1B, DAL1P, Epb4.1l3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13823
HGNC: HGNC:3380
Homologene: 49308
Ddx6
Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
Synonyms: mRCK/P54, HLR2, E230023J21Rik, p54, rck, 1110001P04Rik, C430015D01Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 13209
VEGA: 9
HGNC: HGNC:2747
Homologene: 3238
Kars
Name: lysyl-tRNA synthetase
Synonyms: D8Wsu108e, LysRS, D8Ertd698e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 85305
HGNC: HGNC:6215
Homologene: 4053
Riox2
Name: ribosomal oxygenase 2
Synonyms: 1810047J07Rik, 3830408E23Rik, 2410057H13Rik, Mina
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 67014
Homologene: 12071
Zfp35
Name: zinc finger protein 35
Synonyms: Zfp-35
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 22694
Homologene: 133081
Sars
Name: seryl-aminoacyl-tRNA synthetase
Synonyms: Strs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20226
Homologene: 4751
Boc
Name: biregional cell adhesion molecule-related/down-regulated by oncogenes (Cdon) binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 117606
Homologene: 32819
Nid2
Name: nidogen 2
Synonyms: entactin-2, entactin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 18074
VEGA: 14
Homologene: 40575
Fnip1
Name: folliculin interacting protein 1
Synonyms: A730024A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216742
Homologene: 28173
Bmper
Name: BMP-binding endothelial regulator
Synonyms: Cv2, 3110056H04Rik, CV-2, Crim3, crossveinless-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 73230
VEGA: 9
Homologene: 12494
Psd
Name: pleckstrin and Sec7 domain containing
Synonyms: Efa6, 1110007H17Rik, Psdl, Efa6a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 73728
VEGA: 19
HGNC: HGNC:9507
Homologene: 31115
Wdr95
Name: WD40 repeat domain 95
Synonyms: 4930434E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 381693
Homologene: 124480
Myh11
Name: myosin, heavy polypeptide 11, smooth muscle
Synonyms: smMHC, SM2, SM1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 17880
VEGA: 16
HGNC: HGNC:7569
Homologene: 128512
Otog
Name: otogelin
Synonyms: Otgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18419
HGNC: HGNC:8516
Homologene: 8421
Spink14
Name: serine peptidase inhibitor, Kazal type 14
Synonyms: Gm5505
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 433178
Homologene: 37400
Itgal
Name: integrin alpha L
Synonyms: LFA-1, Cd11a, Ly-21, Ly-15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16408
HGNC: HGNC:6148
Homologene: 1666
Pou6f1
Name: POU domain, class 6, transcription factor 1
Synonyms: 2310038G18Rik, Emb, cns-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 19009
HGNC: HGNC:9224
Homologene: 7703
Olfr923
Name: olfactory receptor 923
Synonyms: GA_x6K02T2PVTD-32530445-32531380, MOR164-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258812
VEGA: 9
Mus81
Name: MUS81 structure-specific endonuclease subunit
Synonyms: 1200008A18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 71711
Homologene: 5725
Ofcc1
Name: orofacial cleft 1 candidate 1
Synonyms: ojoplano, Opo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218165
VEGA: 13
Homologene: 125376
Ifna6
Name: interferon alpha 6
Synonyms: Ifa6, Ifna8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 15969
HGNC: HGNC:5425
Homologene: 68536
Wdr35
Name: WD repeat domain 35
Synonyms: 4930459M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 74682
Homologene: 10814
Tdrd9
Name: tudor domain containing 9
Synonyms: 4930441E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 74691
Homologene: 14311
Prkag2
Name: protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Synonyms: 2410051C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 108099
HGNC: HGNC:9386
Homologene: 136125
Tgm2
Name: transglutaminase 2, C polypeptide
Synonyms: tissue transglutaminase, protein-glutamine gamma-glutamyltransferase, G[a]h, TG C, tTGas, tTG, TG2, TGase2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21817
Homologene: 3391
Odad1
Name: outer dynein arm docking complex subunit 1
Synonyms: Ccdc114
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 211535
Homologene: 128610
Phc3
Name: polyhomeotic 3
Synonyms: EDR3, HPH3, E030046K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 241915
Homologene: 69390
Bag5
Name: BCL2-associated athanogene 5
Synonyms: 1600025G07Rik, 4930405J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 70369
HGNC: HGNC:941
Homologene: 3584
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 158,143,099 bp
  • C to T, chromosome 3 at 30,914,128 bp
  • T to C, chromosome 3 at 108,433,015 bp
  • G to C, chromosome 4 at 88,827,862 bp
  • T to A, chromosome 5 at 25,021,792 bp
  • T to C, chromosome 5 at 44,480,324 bp
  • T to A, chromosome 5 at 149,581,856 bp
  • T to C, chromosome 7 at 45,948,312 bp
  • T to G, chromosome 7 at 46,269,306 bp
  • A to T, chromosome 7 at 126,792,100 bp
  • T to A, chromosome 7 at 127,305,191 bp
  • A to G, chromosome 8 at 111,998,704 bp
  • A to G, chromosome 9 at 23,483,941 bp
  • T to A, chromosome 9 at 38,828,539 bp
  • G to T, chromosome 9 at 44,614,256 bp
  • T to C, chromosome 11 at 54,502,424 bp
  • T to A, chromosome 11 at 69,639,427 bp
  • T to A, chromosome 12 at 9,028,060 bp
  • T to C, chromosome 12 at 111,710,595 bp
  • T to C, chromosome 12 at 112,031,261 bp
  • A to T, chromosome 13 at 40,072,760 bp
  • T to A, chromosome 13 at 40,087,938 bp
  • G to A, chromosome 14 at 19,768,403 bp
  • A to G, chromosome 15 at 100,580,808 bp
  • T to C, chromosome 16 at 14,239,426 bp
  • A to G, chromosome 16 at 44,492,960 bp
  • A to G, chromosome 16 at 59,489,393 bp
  • C to T, chromosome 17 at 69,210,321 bp
  • T to A, chromosome 18 at 24,004,295 bp
  • T to A, chromosome 18 at 44,030,881 bp
  • T to G, chromosome 19 at 5,486,500 bp
  • T to C, chromosome 19 at 37,653,413 bp
  • A to T, chromosome 19 at 45,885,957 bp
  • T to C, chromosome 19 at 46,314,762 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2863 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040453-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.