Strain Name:
C57BL/6J-MtgxR3108Btlr/Mmmh
Stock Number:
040582-MU
Citation ID:
RRID:MMRRC_040582-MU
Other Names:
R3108 (G1), C57BL/6J-MtgxR3108Btlr
Major Collection:

Gene Information

Ap4e1
Name: adaptor-related protein complex AP-4, epsilon 1
Synonyms: 2310033A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 108011
HGNC: HGNC:573
Homologene: 22397
Drd4
Name: dopamine receptor D4
Synonyms: Drd-4, D4R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13491
HGNC: HGNC:3025
Homologene: 20215
Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: separase, SSE, ESP1, PRCE, PRCE, Cerp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Ntrk3
Name: neurotrophic tyrosine kinase, receptor, type 3
Synonyms: TrkC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18213
HGNC: HGNC:8033
Homologene: 49183
Satb1
Name: special AT-rich sequence binding protein 1
Synonyms: 2610306G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20230
Homologene: 2232
Nup155
Name: nucleoporin 155
Synonyms: D930027M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 170762
VEGA: 15
HGNC: HGNC:8063
Homologene: 43155
Cnot1
Name: CCR4-NOT transcription complex, subunit 1
Synonyms: 6030411K04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234594
HGNC: HGNC:7877
Homologene: 9453
Dclk2
Name: doublecortin-like kinase 2
Synonyms: 6330415M09Rik, Click-II, Dcamkl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 70762
Homologene: 69431
Slf1
Name: SMC5-SMC6 complex localization factor 1
Synonyms: C730024G01Rik, 2700017A04Rik, Brctx, Brctd1, Ankrd32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 105377
Homologene: 13000
Unc45a
Name: unc-45 myosin chaperone A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101869
Homologene: 32423
Adcy1
Name: adenylate cyclase 1
Synonyms: AC1, I-AC, D11Bwg1392e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 432530
HGNC: HGNC:232
Homologene: 41419
Ccnb1
Name: cyclin B1
Synonyms: Cycb-4, Ccnb1-rs13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 268697
HGNC: HGNC:1579
Homologene: 68982
Fat1
Name: FAT atypical cadherin 1
Synonyms: mFat1, 2310038E12Rik, Fath
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 14107
HGNC: HGNC:3595
Homologene: 66302
Zfp169
Name: zinc finger protein 169
Synonyms: 4930429A13Rik, 1700025J14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 67911
Homologene: 2572
Pak4
Name: p21 (RAC1) activated kinase 4
Synonyms: 5730488L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 70584
Homologene: 4300
Raph1
Name: Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
Synonyms: C730009O10Rik, 9430025M21Rik, lamellipodin, Lpd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 77300
Homologene: 71030
Lsr
Name: lipolysis stimulated lipoprotein receptor
Synonyms: Lisch7, ILDR3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 54135
Homologene: 9306
Trhde
Name: TRH-degrading enzyme
Synonyms: 9330155P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 237553
Homologene: 75007
Nkpd1
Name: NTPase, KAP family P-loop domain containing 1
Synonyms: 2310015G09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 69547
Homologene: 18876
Dennd4a
Name: DENN/MADD domain containing 4A
Synonyms: F730015K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102442
VEGA: 9
Homologene: 55933
Gm872
Name: predicted gene 872
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Mrgprb1
Name: MAS-related GPR, member B1
Synonyms: MrgB1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233231
Homologene: 24986
Muc5b
Name: mucin 5, subtype B, tracheobronchial
Synonyms: MUC9, MUC5, 2300002I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74180
HGNC: HGNC:7516
Homologene: 136756
Serpina1a
Name: serine (or cysteine) peptidase inhibitor, clade A, member 1A
Synonyms: PI1, Aat-2, Spi1-1, Aat2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 20700
HGNC: HGNC:8941
Homologene: 20103
Olfr591
Name: olfactory receptor 591
Synonyms: GA_x6K02T2PBJ9-5889409-5888465, MOR24-1P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258139
Homologene: 79429
Zfp229
Name: zinc finger protein 229
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 381067
Homologene: 136287
Cd46
Name: CD46 antigen, complement regulatory protein
Synonyms: CD46, Mcp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17221
HGNC: HGNC:6953
Homologene: 7832
Olfr466
Name: olfactory receptor 466
Synonyms: GA_x6K02T2PB7A-3051266-3052192, MOR209-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 258816
Homologene: 72032
Abhd2
Name: abhydrolase domain containing 2
Synonyms: 2210009N18Rik, LABH2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 54608
Homologene: 23121
Slc34a3
Name: solute carrier family 34 (sodium phosphate), member 3
Synonyms: NPTIIc, Npt2c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 142681
Homologene: 15444
Tprkb
Name: Tp53rk binding protein
Synonyms: 0610033G21Rik, DRWMS1, 1810034M08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 69786
Homologene: 41096
Dtx2
Name: deltex 2, E3 ubiquitin ligase
Synonyms: 2610524D08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 74198
Homologene: 56904
Trim30d
Name: tripartite motif-containing 30D
Synonyms: TRIM30-3, Trim79
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 209387
Homologene: 114426
Ston1
Name: stonin 1
Synonyms: 4921524J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 77057
Homologene: 122152
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 60,493,386 bp
  • C to T, chromosome 1 at 195,085,450 bp
  • G to T, chromosome 2 at 25,229,245 bp
  • T to C, chromosome 2 at 127,056,306 bp
  • G to A, chromosome 3 at 86,920,035 bp
  • G to A, chromosome 5 at 136,021,816 bp
  • C to T, chromosome 6 at 85,928,767 bp
  • G to A, chromosome 7 at 19,522,978 bp
  • A to T, chromosome 7 at 28,564,344 bp
  • A to G, chromosome 7 at 30,958,890 bp
  • A to G, chromosome 7 at 48,447,328 bp
  • C to T, chromosome 7 at 78,460,515 bp
  • C to T, chromosome 7 at 79,323,585 bp
  • A to G, chromosome 7 at 80,331,546 bp
  • T to A, chromosome 7 at 103,173,086 bp
  • A to T, chromosome 7 at 104,473,293 bp
  • T to A, chromosome 7 at 141,292,282 bp
  • C to T, chromosome 7 at 141,858,759 bp
  • G to A, chromosome 8 at 45,045,173 bp
  • A to G, chromosome 8 at 95,735,749 bp
  • A to G, chromosome 9 at 64,912,387 bp
  • T to C, chromosome 10 at 92,994,683 bp
  • C to T, chromosome 10 at 114,592,066 bp
  • A to G, chromosome 11 at 7,169,453 bp
  • A to T, chromosome 12 at 103,853,841 bp
  • A to G, chromosome 13 at 48,489,996 bp
  • T to C, chromosome 13 at 65,153,061 bp
  • C to T, chromosome 13 at 77,126,721 bp
  • C to T, chromosome 13 at 100,781,624 bp
  • C to A, chromosome 15 at 8,117,306 bp
  • A to G, chromosome 15 at 102,312,989 bp
  • T to A, chromosome 17 at 21,746,816 bp
  • A to T, chromosome 17 at 51,782,782 bp
  • G to T, chromosome 17 at 88,636,155 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3108 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040582-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.