Strain Name:
C57BL/6J-MtgxR3727Btlr/Mmmh
Stock Number:
040718-MU
Citation ID:
RRID:MMRRC_040718-MU
Other Names:
R3727 (G1), C57BL/6J-MtgxR3727Btlr
Major Collection:

Gene Information

Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11426
Homologene: 136191
Cemip2
Name: cell migration inducing hyaluronidase 2
Synonyms: 3110012M15Rik, Tmem2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 83921
VEGA: 19
Homologene: 75008
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: 6430573H23Rik, Prkwnk1, EG406236, Hsn2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232341
Homologene: 14253
Ttc39b
Name: tetratricopeptide repeat domain 39B
Synonyms: 9130422G05Rik, 1810054D07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 69863
Homologene: 25228
Prpf38a
Name: PRP38 pre-mRNA processing factor 38 (yeast) domain containing A
Synonyms: 2410002M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230596
Homologene: 13146
Kif13b
Name: kinesin family member 13B
Synonyms: GAKIN, N-3 kinesin, C130021D12Rik, 5330429L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 16554
VEGA: 14
Homologene: 9073
Dpp3
Name: dipeptidylpeptidase 3
Synonyms: 4930533O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 75221
VEGA: 19
HGNC: HGNC:3008
Homologene: 40210
Zmym2
Name: zinc finger, MYM-type 2
Synonyms: SCLL, RAMP, MYM, FIM, Zfp198
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 76007
VEGA: 14
Homologene: 12631
Ago2
Name: argonaute RISC catalytic subunit 2
Synonyms: argonaute 2, 1110029L17Rik, 2310051F07Rik, Eif2c2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239528
HGNC: HGNC:3263
Homologene: 81825
C3
Name: complement component 3
Synonyms: complement factor 3, acylation stimulating protein, Plp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12266
HGNC: HGNC:1318
Homologene: 68031
Nfix
Name: nuclear factor I/X
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18032
HGNC: HGNC:7788
Homologene: 1872
Eps15
Name: epidermal growth factor receptor pathway substrate 15
Synonyms: 2410112D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13858
HGNC: HGNC:3419
Homologene: 128359
Dnah8
Name: dynein, axonemal, heavy chain 8
Synonyms: Hst6.7b, P1-Loop, Dnahc8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13417
VEGA: 17
HGNC: HGNC:2952
Homologene: 1049
Ifrd2
Name: interferon-related developmental regulator 2
Synonyms: SKMc15, 1810034A24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 15983
HGNC: HGNC:5457
Homologene: 21341
Exosc10
Name: exosome component 10
Synonyms: PM/Scl-100, PM-Scl, RRP6, p4, p3, p2, Pmscl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 50912
HGNC: HGNC:9138
Homologene: 31105
Dusp27
Name: dual specificity phosphatase 27 (putative)
Synonyms: C130085G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240892
Homologene: 18949
Flt3
Name: FMS-like tyrosine kinase 3
Synonyms: CD135, Flk-2, Flt-3, Flk2, wmfl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14255
HGNC: HGNC:3765
Homologene: 3040
Stpg2
Name: sperm tail PG rich repeat containing 2
Synonyms: LOC381476, B930007M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 381476
Homologene: 52167
Olfr137
Name: olfactory receptor 137
Synonyms: MOR256-18, GA_x6K02T2PSCP-2749525-2748587
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258481
HGNC: HGNC:8261
Homologene: 128084
Ina
Name: internexin neuronal intermediate filament protein, alpha
Synonyms: NF-66, NF66
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226180
HGNC: HGNC:6057
Homologene: 10433
Pdzrn3
Name: PDZ domain containing RING finger 3
Synonyms: semaphorin cytoplasmic domain-associated protein 3A, 1110020C07Rik, LNX3, Semcap3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 55983
Homologene: 10328
Rab42
Name: RAB42, member RAS oncogene family
Synonyms: 9530096D07Rik, Rab42-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242681
Homologene: 85237
Hormad2
Name: HORMA domain containing 2
Synonyms: 4930529M09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75828
Homologene: 45130
Oas1h
Name: 2'-5' oligoadenylate synthetase 1H
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 246729
HGNC: HGNC:8086
Homologene: 77345
Atp6v0a1
Name: ATPase, H+ transporting, lysosomal V0 subunit A1
Synonyms: V-ATPase a1, Vpp1, Vpp-1, Atp6n1, Atp6n1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11975
HGNC: HGNC:865
Homologene: 3795
Ccng2
Name: cyclin G2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12452
HGNC: HGNC:1593
Homologene: 3208
Esp6
Name: exocrine gland secreted peptide 6
Synonyms: Esp5, Gm5494
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 433107
VEGA: 17
Alk
Name: anaplastic lymphoma kinase
Synonyms: Tcrz, CD246
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 11682
VEGA: 17
HGNC: HGNC:427
Homologene: 68387
Slc52a3
Name: solute carrier protein family 52, member 3
Synonyms: 2310046K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 69698
Homologene: 12324
Vmn2r22
Name: vomeronasal 2, receptor 22
Synonyms: EG546913
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 546913
Homologene: 84037
Nt5c
Name: 5',3'-nucleotidase, cytosolic
Synonyms: Umph-2, Dnt1, Dnt, Umph2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 50773
Homologene: 8745
Vmn2r95
Name: vomeronasal 2, receptor 95
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 328759
Homologene: 115024
Olfr1391
Name: olfactory receptor 1391
Synonyms: GA_x6K02T2QP88-6107233-6106298, MOR256-27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258460
Homologene: 17264
Cyp2c38
Name: cytochrome P450, family 2, subfamily c, polypeptide 38
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 13097
HGNC: HGNC:2622
Homologene: 117948
Dmgdh
Name: dimethylglycine dehydrogenase precursor
Synonyms: 1200014D15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 74129
Homologene: 8372
Mpi
Name: mannose phosphate isomerase
Synonyms: Mpi-1, 1110002E17Rik, Mpi1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 110119
HGNC: HGNC:7216
Homologene: 1825
Rpia
Name: ribose 5-phosphate isomerase A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 19895
Homologene: 6943
BC035044
Name: cDNA sequence BC035044
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232406
Tmem240
Name: transmembrane protein 240
Synonyms: Gm5151
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381582
Homologene: 19885
Caly
Name: calcyon neuron-specific vesicular protein
Synonyms: 0710001P07Rik, 1110004A22Rik, Calcyon, Drd1ip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68566
Homologene: 9256
Cypt1
Name: cysteine-rich perinuclear theca 1
Synonyms: TISP55, Ckt1r3, 4931420D14Rik, CYPT1, Cypt6, Cypt5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 66742
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 166,099,506 bp
  • T to C, chromosome 2 at 69,510,429 bp
  • C to T, chromosome 2 at 152,005,781 bp
  • T to C, chromosome 3 at 139,298,496 bp
  • A to T, chromosome 4 at 83,263,680 bp
  • A to G, chromosome 4 at 108,568,480 bp
  • G to T, chromosome 4 at 109,370,685 bp
  • T to A, chromosome 4 at 123,459,311 bp
  • A to G, chromosome 4 at 132,302,653 bp
  • A to G, chromosome 4 at 148,565,277 bp
  • T to C, chromosome 4 at 155,739,778 bp
  • T to C, chromosome 5 at 93,274,951 bp
  • T to A, chromosome 5 at 120,862,519 bp
  • C to A, chromosome 5 at 147,354,923 bp
  • A to T, chromosome 6 at 70,777,421 bp
  • T to A, chromosome 6 at 101,156,945 bp
  • T to C, chromosome 6 at 119,992,453 bp
  • A to G, chromosome 6 at 123,650,625 bp
  • C to A, chromosome 6 at 128,890,859 bp
  • T to C, chromosome 7 at 140,070,504 bp
  • CAAAAA to CAAAA, chromosome 8 at 84,716,247 bp
  • T to C, chromosome 9 at 57,544,849 bp
  • C to T, chromosome 9 at 107,591,682 bp
  • C to A, chromosome 11 at 4,408,598 bp
  • G to A, chromosome 11 at 49,327,795 bp
  • T to A, chromosome 11 at 101,030,420 bp
  • C to T, chromosome 11 at 115,490,648 bp
  • A to G, chromosome 13 at 93,692,067 bp
  • A to T, chromosome 14 at 56,919,349 bp
  • A to G, chromosome 14 at 64,765,748 bp
  • C to T, chromosome 15 at 73,113,857 bp
  • C to T, chromosome 17 at 18,441,482 bp
  • C to T, chromosome 17 at 30,739,648 bp
  • A to T, chromosome 17 at 38,305,419 bp
  • T to C, chromosome 17 at 40,576,615 bp
  • G to T, chromosome 17 at 57,207,379 bp
  • T to C, chromosome 17 at 71,901,400 bp
  • A to G, chromosome 19 at 4,923,185 bp
  • G to A, chromosome 19 at 21,844,711 bp
  • A to G, chromosome 19 at 39,392,295 bp
  • G to A, chromosome 19 at 47,015,719 bp
  • T to A, chromosome X at 16,523,435 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3727 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040718-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.