Strain Name:
C57BL/6J-MtgxR3821Btlr/Mmmh
Stock Number:
040883-MU
Citation ID:
RRID:MMRRC_040883-MU
Other Names:
R3821 (G1), C57BL/6J-MtgxR3821Btlr
Major Collection:

Strain Information

Erbb4
Name: erb-b2 receptor tyrosine kinase 4
Synonyms: ErbB4, Her4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13869
HGNC: HGNC:3432
Homologene: 21084
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: b2b1562Clo, ne, my, 8430406N05Rik, 6030440P17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242022
Homologene: 18454
Ufc1
Name: ubiquitin-fold modifier conjugating enzyme 1
Synonyms: ESTM29, 1110021H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66155
Homologene: 9500
Htr1b
Name: 5-hydroxytryptamine (serotonin) receptor 1B
Synonyms: 5-HT1B receptor, 5-HT1B receptor, 5HT1B receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 15551
HGNC: HGNC:5287
Homologene: 669
Kdm3a
Name: lysine (K)-specific demethylase 3A
Synonyms: Jmjd1a, Jmjd1, 1700105C21Rik, Tsga, C230043E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 104263
Homologene: 10196
Cnot6
Name: CCR4-NOT transcription complex, subunit 6
Synonyms: A230103N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 104625
Homologene: 69187
Anp32e
Name: acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
Synonyms: CPD1, 2810018A15Rik, LANP-L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 66471
Homologene: 41519
Ptprg
Name: protein tyrosine phosphatase, receptor type, G
Synonyms: 5430405N12Rik, RPTPgamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 19270
HGNC: HGNC:9671
Homologene: 2129
Gbf1
Name: golgi-specific brefeldin A-resistance factor 1
Synonyms: 1700083E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107338
HGNC: HGNC:4181
Homologene: 37897
Tenm2
Name: teneurin transmembrane protein 2
Synonyms: 2610040L17Rik, 9330187F13Rik, Odz2, Ten-m2, D3Bwg1534e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 23964
Homologene: 22672
Marf1
Name: meiosis regulator and mRNA stability 1
Synonyms: 4921513D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 223989
VEGA: 16
Homologene: 40967
Cramp1
Name: cramped chromatin regulator 1
Synonyms: Cramp1l, 5830477H08Rik, Tce4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 57354
VEGA: 17
Homologene: 35373
Sdhb
Name: succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
Synonyms: 0710008N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67680
Homologene: 2255
C6
Name: complement component 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 12274
HGNC: HGNC:1339
Homologene: 47
Scn10a
Name: sodium channel, voltage-gated, type X, alpha
Synonyms: Nav1.8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20264
VEGA: 9
Homologene: 21300
Wdfy1
Name: WD repeat and FYVE domain containing 1
Synonyms: Jr1, 1700013B03Rik, 1700120F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 69368
Homologene: 10825
Tmem131
Name: transmembrane protein 131
Synonyms: Rw1, CC28, D1Bwg0491e, Neg, 2610524E03Rik, YR-23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 56030
Homologene: 32428
Lhfpl4
Name: lipoma HMGIC fusion partner-like protein 4
Synonyms: 1190004M23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 269788
Homologene: 14943
Ubr3
Name: ubiquitin protein ligase E3 component n-recognin 3
Synonyms: 1110059H15Rik, Zfp650, A130030D10Rik, 4833421P10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68795
Homologene: 52092
Aarsd1
Name: alanyl-tRNA synthetase domain containing 1
Synonyms: 2310044P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69684
Homologene: 6821
Slitrk3
Name: SLIT and NTRK-like family, member 3
Synonyms: ST3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 386750
Homologene: 8955
Fchsd1
Name: FCH and double SH3 domains 1
Synonyms: A030002D08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 319262
Homologene: 14127
Neto2
Name: neuropilin (NRP) and tolloid (TLL)-like 2
Synonyms: 5530601C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 74513
Homologene: 32387
Slc20a2
Name: solute carrier family 20, member 2
Synonyms: Pit-2, Ram-1, PiT-2, Ram1, MolPit2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 20516
Homologene: 68531
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Eml2
Name: echinoderm microtubule associated protein like 2
Synonyms: 1600029N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72205
Homologene: 8125
Mns1
Name: meiosis-specific nuclear structural protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17427
Homologene: 40628
Trpc6
Name: transient receptor potential cation channel, subfamily C, member 6
Synonyms: mtrp6, Trrp6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 22068
VEGA: 9
Homologene: 37944
Col9a2
Name: collagen, type IX, alpha 2
Synonyms: Col9a-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12840
HGNC: HGNC:2218
Homologene: 37535
Itgam
Name: integrin alpha M
Synonyms: Ly-40, complement receptor type 3, Mac-1 alpha, complement component receptor 3 alpha, CD11B (p170), Mac-1, Mac-1a, F730045J24Rik, Cd11b, CR3, CD11b/CD18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16409
HGNC: HGNC:6149
Homologene: 526
Dnah5
Name: dynein, axonemal, heavy chain 5
Synonyms: b2b1565Clo, b2b1154Clo, Mdnah5, b2b1537Clo, Dnahc5, b2b3491Clo, b2b1134Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 110082
HGNC: HGNC:2950
Homologene: 1048
Adamtsl3
Name: ADAMTS-like 3
Synonyms: punctin-2, 9230119C12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269959
Homologene: 18912
Zfp831
Name: zinc finger protein 831
Synonyms: ENSMUSG00000050600, OTTMUSG00000017459
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 100043757
Homologene: 19013
Itpr2
Name: inositol 1,4,5-triphosphate receptor 2
Synonyms: Itpr5, Ip3r2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16439
HGNC: HGNC:6181
Homologene: 37593
Tnfsf8
Name: tumor necrosis factor (ligand) superfamily, member 8
Synonyms: CD153, CD30LG, Cd30L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21949
Homologene: 950
Ncoa6
Name: nuclear receptor coactivator 6
Synonyms: ASC-2, AIB3, RAP250, NRC, PRIP, ASC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56406
Homologene: 137337
Ralgapa2
Name: Ral GTPase activating protein, alpha subunit 2 (catalytic)
Synonyms: RGC2, AS250, A230067G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241694
Homologene: 28131
Tmem209
Name: transmembrane protein 209
Synonyms: 2700094F01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 72649
Homologene: 18021
Irs1
Name: insulin receptor substrate 1
Synonyms: G972R, IRS-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16367
HGNC: HGNC:6125
Homologene: 4049
Dnah9
Name: dynein, axonemal, heavy chain 9
Synonyms: Dnahc9, D11Ertd686e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237806
HGNC: HGNC:2953
Homologene: 20357
Trank1
Name: tetratricopeptide repeat and ankyrin repeat containing 1
Synonyms: A230061D21Rik, Lba1, LOC235639, C030048J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 320429
Homologene: 45845
Flad1
Name: flavin adenine dinucleotide synthetase 1
Synonyms: Pp591, A930017E24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 319945
Homologene: 115598
Kcnma1
Name: potassium large conductance calcium-activated channel, subfamily M, alpha member 1
Synonyms: mSlo1, MaxiK, BK channel alpha subunit, Slo, 5730414M22Rik, BKCa, Slo1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 16531
HGNC: HGNC:6284
Homologene: 1693
Acpp
Name: acid phosphatase, prostate
Synonyms: PAP, A030005E02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 56318
HGNC: HGNC:125
Homologene: 55552
Topaz1
Name: testis and ovary specific PAZ domain containing 1
Synonyms: Gm9524
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 671232
VEGA: 9
Homologene: 19835
Tmbim1
Name: transmembrane BAX inhibitor motif containing 1
Synonyms: 2310061B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 69660
Homologene: 36404
Ccdc13
Name: coiled-coil domain containing 13
Synonyms: 2900041A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 100502861
Homologene: 87213
Mrc2
Name: mannose receptor, C type 2
Synonyms: uPARAP, Endo180, novel lectin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17534
Homologene: 4408
Usb1
Name: U6 snRNA biogenesis 1
Synonyms: AA960436
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 101985
Homologene: 11611
Hscb
Name: HscB iron-sulfur cluster co-chaperone
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100900
Homologene: 13945
Zfp618
Name: zinc finger protein 618
Synonyms: D430033D05Rik, Nedd10, 2810040O04Rik, 2810031P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 72701
Homologene: 18975
Ptprb
Name: protein tyrosine phosphatase, receptor type, B
Synonyms: VE-PTP, 3230402H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19263
VEGA: 10
HGNC: HGNC:9665
Homologene: 2125
Insyn2b
Name: inhibitory synaptic factor family member 2B
Synonyms: Gm6041, Fam196b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 574403
VEGA: 11
Homologene: 107175
Tmem8b
Name: transmembrane protein 8B
Synonyms: 4930500O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242409
Homologene: 72894
Pde8b
Name: phosphodiesterase 8B
Synonyms: B230331L10Rik, C030047E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218461
HGNC: HGNC:8794
Homologene: 2758
Hoxc9
Name: homeobox C9
Synonyms: Hox-3.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 15427
HGNC: HGNC:5130
Homologene: 130558
Ido2
Name: indoleamine 2,3-dioxygenase 2
Synonyms: Indol1, Ido2, C230043N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 209176
Homologene: 48830
Or2a51
Name: olfactory receptor family 2 subfamily A member 51
Synonyms: GA_x6K02T2P3E9-4356476-4355535, MOR261-5, Olfr435
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 258647
Homologene: 110586
Rab40b
Name: Rab40B, member RAS oncogene family
Synonyms: rar
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217371
Homologene: 4968
Abcd2
Name: ATP-binding cassette, sub-family D (ALD), member 2
Synonyms: ABC39, adrenoleukodystrophy related, ALDL1, ALDR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 26874
VEGA: 15
HGNC: HGNC:66
Homologene: 55873
Ehd3
Name: EH-domain containing 3
Synonyms: Ehd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 57440
HGNC: HGNC:3244
Homologene: 81837
Mrpl19
Name: mitochondrial ribosomal protein L19
Synonyms: D6Ertd157e, Rpml15, MRP-L15, 9030416F12Rik, RLX1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 56284
Homologene: 8851
Alox8
Name: arachidonate 8-lipoxygenase
Synonyms: 8-LOX, 8S-LOX, Alox15b, 8S-lipoxygenase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11688
HGNC: HGNC:434
Homologene: 886
Clgn
Name: calmegin
Synonyms: A2/6, 4930459O04Rik, calnexin-t, Cln
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12745
HGNC: HGNC:2060
Homologene: 68392
Slc17a4
Name: solute carrier family 17 (sodium phosphate), member 4
Synonyms: 9130214H05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 319848
Homologene: 21127
Tas1r1
Name: taste receptor, type 1, member 1
Synonyms: T1R1, T1r1, Gpr70, TR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 110326
Homologene: 12888
Arpin
Name: actin-related protein 2/3 complex inhibitor
Synonyms: 2610034B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 70420
Homologene: 128480
Unc5cl
Name: unc-5 family C-terminal like
Synonyms: 2510009H09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 76589
Homologene: 18319
Shroom4
Name: shroom family member 4
Synonyms: D430043L16Rik, Shrm4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 208431
Homologene: 26615
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 36,808,396 bp
  • A to G, chromosome 1 at 68,305,913 bp
  • A to G, chromosome 1 at 74,293,930 bp
  • A to G, chromosome 1 at 79,706,300 bp
  • T to C, chromosome 1 at 82,290,049 bp
  • A to G, chromosome 1 at 171,289,599 bp
  • G to A, chromosome 2 at 69,993,813 bp
  • T to C, chromosome 2 at 146,449,195 bp
  • T to A, chromosome 2 at 155,406,938 bp
  • T to C, chromosome 2 at 174,644,023 bp
  • A to T, chromosome 3 at 53,652,415 bp
  • T to C, chromosome 3 at 73,049,216 bp
  • T to A, chromosome 3 at 89,411,187 bp
  • A to T, chromosome 3 at 95,934,869 bp
  • C to A, chromosome 4 at 43,689,745 bp
  • G to A, chromosome 4 at 63,133,564 bp
  • A to G, chromosome 4 at 63,860,890 bp
  • C to G, chromosome 4 at 121,054,258 bp
  • C to T, chromosome 4 at 140,979,088 bp
  • G to T, chromosome 4 at 152,034,681 bp
  • A to T, chromosome 5 at 110,836,328 bp
  • G to T, chromosome 6 at 30,505,960 bp
  • A to G, chromosome 6 at 43,201,670 bp
  • A to T, chromosome 6 at 71,611,677 bp
  • C to A, chromosome 6 at 81,962,006 bp
  • A to G, chromosome 6 at 113,194,108 bp
  • A to T, chromosome 6 at 146,417,726 bp
  • T to C, chromosome 7 at 19,202,986 bp
  • G to A, chromosome 7 at 79,929,660 bp
  • G to T, chromosome 7 at 82,606,479 bp
  • A to G, chromosome 7 at 128,112,286 bp
  • A to G, chromosome 8 at 22,538,902 bp
  • T to C, chromosome 8 at 24,533,755 bp
  • A to G, chromosome 8 at 83,420,477 bp
  • C to A, chromosome 8 at 85,663,295 bp
  • G to A, chromosome 8 at 95,333,433 bp
  • A to T, chromosome 9 at 8,610,278 bp
  • A to G, chromosome 9 at 72,439,448 bp
  • A to T, chromosome 9 at 81,632,434 bp
  • G to T, chromosome 9 at 104,324,717 bp
  • G to A, chromosome 9 at 111,378,819 bp
  • A to G, chromosome 9 at 119,638,633 bp
  • C to A, chromosome 9 at 121,831,019 bp
  • A to G, chromosome 9 at 122,797,783 bp
  • G to T, chromosome 10 at 116,350,074 bp
  • T to C, chromosome 11 at 34,403,007 bp
  • A to T, chromosome 11 at 36,024,320 bp
  • A to T, chromosome 11 at 49,689,172 bp
  • C to A, chromosome 11 at 65,851,003 bp
  • T to A, chromosome 11 at 69,186,482 bp
  • T to A, chromosome 11 at 101,411,145 bp
  • G to A, chromosome 11 at 105,348,431 bp
  • T to A, chromosome 11 at 121,358,048 bp
  • C to T, chromosome 13 at 23,901,769 bp
  • A to G, chromosome 13 at 95,107,855 bp
  • A to T, chromosome 14 at 12,226,375 bp
  • A to G, chromosome 14 at 23,367,611 bp
  • A to G, chromosome 15 at 4,789,584 bp
  • T to C, chromosome 15 at 28,340,437 bp
  • C to T, chromosome 15 at 91,174,705 bp
  • A to G, chromosome 15 at 102,982,164 bp
  • A to T, chromosome 16 at 14,142,554 bp
  • G to A, chromosome 17 at 24,974,782 bp
  • T to C, chromosome 17 at 48,459,973 bp
  • C to A, chromosome 17 at 67,779,046 bp
  • A to T, chromosome 17 at 73,827,395 bp
  • A to G, chromosome 18 at 37,969,457 bp
  • T to C, chromosome 19 at 46,264,807 bp
  • C to T, chromosome X at 6,624,222 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3821 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040883-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.