Strain Name:
C57BL/6J-MtgxR3907Btlr/Mmmh
Stock Number:
040908-MU
Citation ID:
RRID:MMRRC_040908-MU
Other Names:
R3907 (G1), C57BL/6J-MtgxR3907Btlr
Major Collection:

Gene Information

Kitl
Name: kit ligand
Synonyms: Gb, SCF, Steel, Mgf, Sl, Kitlg, SF, grizzle-belly, blz, stem cell factor, Steel factor, SLF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 17311
HGNC: HGNC:6343
Homologene: 692
Utrn
Name: utrophin
Synonyms: G-utrophin, DRP, Dmdl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 22288
VEGA: 10
Homologene: 21398
Fn1
Name: fibronectin 1
Synonyms: Fn-1, Fn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 14268
HGNC: HGNC:3778
Homologene: 1533
Chrm4
Name: cholinergic receptor, muscarinic 4
Synonyms: Chrm-4, muscarinic acetylcholine receptor 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 12672
HGNC: HGNC:1953
Homologene: 20192
Ppp1r16b
Name: protein phosphatase 1, regulatory subunit 16B
Synonyms: C130078N17Rik, Wdt4, ANKRD4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228852
Homologene: 9194
Anks3
Name: ankyrin repeat and sterile alpha motif domain containing 3
Synonyms: 2700067D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 72615
VEGA: 16
Homologene: 12475
Arid1a
Name: AT rich interactive domain 1A (SWI-like)
Synonyms: Osa1, Smarcf1, 1110030E03Rik, BAF250a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 93760
Homologene: 21216
Hars
Name: histidyl-tRNA synthetase
Synonyms: MMHRS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 15115
VEGA: 18
HGNC: HGNC:4816
Homologene: 1592
Gphn
Name: gephyrin
Synonyms: 5730552E08Rik, geph
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 268566
VEGA: 12
Homologene: 10820
Tsc22d1
Name: TSC22 domain family, member 1
Synonyms: TSC-22, Egr5, Tgfb1i4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 21807
Homologene: 7573
Trip12
Name: thyroid hormone receptor interactor 12
Synonyms: 6720416K24Rik, Gtl6, 1110036I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 14897
Homologene: 44226
Rcan1
Name: regulator of calcineurin 1
Synonyms: ADAPT78, MCIP1, CSP1, 2410048A02Rik, Dscr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 54720
VEGA: 16
HGNC: HGNC:3040
Homologene: 3251
Cct4
Name: chaperonin containing Tcp1, subunit 4 (delta)
Synonyms: A45, 2610204B21Rik, Cctd, T complex protein 1, delta, TCP-1 delta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 12464
HGNC: HGNC:1617
Homologene: 4695
Ptpn6
Name: protein tyrosine phosphatase, non-receptor type 6
Synonyms: Ptp1C, SHP-1, Hcph, hcp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 15170
HGNC: HGNC:9658
Homologene: 56589
Iws1
Name: IWS1, SUPT6 interacting protein
Synonyms: 1700069O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 73473
Homologene: 134421
Fat1
Name: FAT atypical cadherin 1
Synonyms: mFat1, 2310038E12Rik, Fath
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 14107
HGNC: HGNC:3595
Homologene: 66302
Copg1
Name: coatomer protein complex, subunit gamma 1
Synonyms: D6Ertd71e, D6Wsu16e, Copg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 54161
HGNC: HGNC:2236
Homologene: 56745
Dcbld1
Name: discoidin, CUB and LCCL domain containing 1
Synonyms: 4631413K11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 66686
Homologene: 12010
Asph
Name: aspartate-beta-hydroxylase
Synonyms: Junctin, cI-37, junctate, calsequestrin-binding protein, aspartyl beta-hydroxylase, 3110001L23Rik, jumbug, 2310005F16Rik, BAH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 65973
HGNC: HGNC:757
Homologene: 20910
Pwwp3a
Name: PWWP domain containing 3A, DNA repair factor
Synonyms: 9430059D04Rik, Mum1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 68114
Homologene: 11349
Stn1
Name: STN1, CST complex subunit
Synonyms: 0610009H20Rik, Obfc1, 2310057J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 108689
Homologene: 11788
Dcaf6
Name: DDB1 and CUL4 associated factor 6
Synonyms: Iqwd1, 1200006M05Rik, PC326
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 74106
Homologene: 10199
Usp54
Name: ubiquitin specific peptidase 54
Synonyms: 4930429G18Rik, C030002J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 78787
Homologene: 90902
Trhde
Name: TRH-degrading enzyme
Synonyms: 9330155P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 237553
Homologene: 75007
Atp2b4
Name: ATPase, Ca++ transporting, plasma membrane 4
Synonyms: PMCA4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 381290
HGNC: HGNC:817
Homologene: 48034
Foxp2
Name: forkhead box P2
Synonyms: D0Kist7, 2810043D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 114142
Homologene: 134404
Tespa1
Name: thymocyte expressed, positive selection associated 1
Synonyms: Itprid3, 5830405N20Rik, A430001F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 67596
VEGA: 10
Homologene: 106645
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Arsi
Name: arylsulfatase i
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 545260
Homologene: 44428
Loxhd1
Name: lipoxygenase homology domains 1
Synonyms: 1700096C21Rik, sba
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 240411
Car4
Name: carbonic anhydrase 4
Synonyms: CA IV
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 12351
HGNC: HGNC:1375
Homologene: 20183
Mapkapk2
Name: MAP kinase-activated protein kinase 2
Synonyms: Rps6kc1, MAPKAP kinase 2, MK2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 17164
HGNC: HGNC:6887
Homologene: 56412
Ampd3
Name: adenosine monophosphate deaminase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 11717
HGNC: HGNC:470
Homologene: 408
Csf3r
Name: colony stimulating factor 3 receptor (granulocyte)
Synonyms: Cd114, Csfgr, G-CSFR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 12986
HGNC: HGNC:2439
Homologene: 601
Rnf185
Name: ring finger protein 185
Synonyms: 1700022N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 193670
Homologene: 34298
Cacna1s
Name: calcium channel, voltage-dependent, L type, alpha 1S subunit
Synonyms: sj, DHPR alpha1s, muscle dysgenesis, fmd, Cchl1a3, mdg, Cav1.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12292
HGNC: HGNC:1397
Homologene: 37257
4930402H24Rik
Name: RIKEN cDNA 4930402H24 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228602
Homologene: 12623
Asns
Name: asparagine synthetase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 27053
HGNC: HGNC:753
Homologene: 69113
Ugt8a
Name: UDP galactosyltransferase 8A
Synonyms: Cgt, mCerGT, Ugt8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 22239
Homologene: 20715
Adamts3
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 3
Synonyms: 1100001H14Rik, 6330442E02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 330119
HGNC: HGNC:219
Homologene: 8596
Duox2
Name: dual oxidase 2
Synonyms: A430065P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 214593
Homologene: 9689
Apba1
Name: amyloid beta (A4) precursor protein binding, family A, member 1
Synonyms: X11, X11alpha, Mint1, Lin-10, 6430513E09Rik, Mint
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 319924
VEGA: 19
HGNC: HGNC:578
Homologene: 897
Otoa
Name: otoancorin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 246190
Homologene: 71803
Shank2
Name: SH3 and multiple ankyrin repeat domains 2
Synonyms: ProSAP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 210274
Homologene: 105965
Plekha4
Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4
Synonyms: 2410005C22Rik, PEPP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 69217
Homologene: 10848
Ephb1
Name: Eph receptor B1
Synonyms: C130099E04Rik, Hek6, Cek6, Net, Elkh, Elk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 270190
HGNC: HGNC:3392
Homologene: 20936
Fam76a
Name: family with sequence similarity 76, member A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230789
Homologene: 27071
Tmcc2
Name: transmembrane and coiled-coil domains 2
Synonyms: 1110063G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 68875
Homologene: 8905
Prrt4
Name: proline-rich transmembrane protein 4
Synonyms: D330027H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 101359
Homologene: 46116
Krt16
Name: keratin 16
Synonyms: K16, Krt1-16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 16666
HGNC: HGNC:6423
Homologene: 21145
Nlrp5
Name: NLR family, pyrin domain containing 5
Synonyms: Mater, Op1, Nalp5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 23968
Homologene: 65105
Defb4
Name: defensin beta 4
Synonyms: 2310001F05Rik, MBD-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 56519
Taar7a
Name: trace amine-associated receptor 7A
Synonyms: Taar7a, LOC215856
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 215856
Homologene: 110833
Slc19a3
Name: solute carrier family 19, member 3
Synonyms: ThTr2, A230084E24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 80721
Homologene: 23530
Eef2k
Name: eukaryotic elongation factor-2 kinase
Synonyms: eEF-2K
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 13631
Homologene: 7299
Aspg
Name: asparaginase
Synonyms: A530050D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 104816
VEGA: 12
Homologene: 113390
Gm10110
Name: predicted gene 10110
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
Oxct1
Name: 3-oxoacid CoA transferase 1
Synonyms: 2610008O03Rik, Scot-s
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 67041
HGNC: HGNC:8527
Homologene: 377
Olfr5
Name: olfactory receptor 5
Synonyms: MOR103-8, GA_x6K02T2QGBW-3210997-3210059
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 18349
Homologene: 72037
Kcnj4
Name: potassium inwardly-rectifying channel, subfamily J, member 4
Synonyms: Kcnf2, IRK3, MB-IRK3, Kir 2.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 16520
VEGA: 15
HGNC: HGNC:6265
Homologene: 3653
Mxd1
Name: MAX dimerization protein 1
Synonyms: Mad, Mad1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 17119
HGNC: HGNC:6761
Homologene: 1767
Pced1b
Name: PC-esterase domain containing 1B
Synonyms: Fam113b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 239647
VEGA: 15
Homologene: 16301
Olfr1222
Name: olfactory receptor 1222
Synonyms: GA_x6K02T2Q125-50604368-50603433, MOR233-14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258177
Homologene: 73988
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 71,607,913 bp
  • G to A, chromosome 1 at 83,014,813 bp
  • T to C, chromosome 1 at 84,732,106 bp
  • A to T, chromosome 1 at 131,056,914 bp
  • T to C, chromosome 1 at 132,360,638 bp
  • A to T, chromosome 1 at 133,738,586 bp
  • T to A, chromosome 1 at 136,084,269 bp
  • A to T, chromosome 1 at 165,424,380 bp
  • C to A, chromosome 2 at 76,903,342 bp
  • A to C, chromosome 2 at 89,125,583 bp
  • C to T, chromosome 2 at 91,927,739 bp
  • C to T, chromosome 2 at 122,283,060 bp
  • C to T, chromosome 2 at 130,736,576 bp
  • T to C, chromosome 2 at 158,761,490 bp
  • T to C, chromosome 3 at 125,914,982 bp
  • T to C, chromosome 4 at 9,474,934 bp
  • A to T, chromosome 4 at 126,034,447 bp
  • T to C, chromosome 4 at 132,916,121 bp
  • T to C, chromosome 4 at 133,692,912 bp
  • C to A, chromosome 5 at 89,861,355 bp
  • C to T, chromosome 6 at 7,682,270 bp
  • T to C, chromosome 6 at 15,411,229 bp
  • G to T, chromosome 6 at 29,177,174 bp
  • G to T, chromosome 6 at 86,650,960 bp
  • T to C, chromosome 6 at 87,892,195 bp
  • T to C, chromosome 6 at 124,725,276 bp
  • A to T, chromosome 7 at 6,480,679 bp
  • T to A, chromosome 7 at 23,433,646 bp
  • T to C, chromosome 7 at 45,553,557 bp
  • A to G, chromosome 7 at 110,793,670 bp
  • T to C, chromosome 7 at 120,885,799 bp
  • A to T, chromosome 7 at 121,125,565 bp
  • C to T, chromosome 7 at 144,409,576 bp
  • A to T, chromosome 8 at 19,201,261 bp
  • G to C, chromosome 8 at 45,023,035 bp
  • A to G, chromosome 9 at 102,001,726 bp
  • C to T, chromosome 10 at 12,710,182 bp
  • T to C, chromosome 10 at 23,992,559 bp
  • T to G, chromosome 10 at 52,319,470 bp
  • T to C, chromosome 10 at 80,238,316 bp
  • A to C, chromosome 10 at 100,079,960 bp
  • C to T, chromosome 10 at 114,800,696 bp
  • T to C, chromosome 10 at 130,356,797 bp
  • A to G, chromosome 11 at 3,426,681 bp
  • A to G, chromosome 11 at 23,001,560 bp
  • G to A, chromosome 11 at 84,964,357 bp
  • A to G, chromosome 11 at 100,247,163 bp
  • T to A, chromosome 12 at 78,493,942 bp
  • T to A, chromosome 12 at 112,112,259 bp
  • A to T, chromosome 14 at 20,586,113 bp
  • T to C, chromosome 14 at 76,416,543 bp
  • T to C, chromosome 14 at 89,898,147 bp
  • G to A, chromosome 15 at 4,101,117 bp
  • G to T, chromosome 15 at 79,485,745 bp
  • T to C, chromosome 15 at 97,384,550 bp
  • A to T, chromosome 16 at 4,942,304 bp
  • A to G, chromosome 16 at 92,466,029 bp
  • G to A, chromosome 18 at 32,079,920 bp
  • A to T, chromosome 18 at 36,782,716 bp
  • G to A, chromosome 18 at 60,916,651 bp
  • A to T, chromosome 18 at 77,408,768 bp
  • T to C, chromosome 19 at 23,937,506 bp
  • T to C, chromosome 19 at 47,507,823 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3907 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040908-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.