Strain Name:
C57BL/6J-MtgxR4058Btlr/Mmmh
Stock Number:
040969-MU
Citation ID:
RRID:MMRRC_040969-MU
Other Names:
R4058 (G1), C57BL/6J-MtgxR4058Btlr
Major Collection:

Gene Information

Rgs8
Name: regulator of G-protein signaling 8
Synonyms: 6530413N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67792
Homologene: 23152
Tns3
Name: tensin 3
Synonyms: Tens1, F830010I22Rik, TEM6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 319939
Homologene: 49713
Taok1
Name: TAO kinase 1
Synonyms: D130018F14Rik, 2810468K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216965
Homologene: 27041
Anxa4
Name: annexin A4
Synonyms: annexin IV, AIV, Anx4, Xanx-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 11746
HGNC: HGNC:542
Homologene: 68164
Prep
Name: prolyl endopeptidase
Synonyms: Pop, D10Wsu136e, prolyl oligopeptidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19072
VEGA: 10
HGNC: HGNC:9358
Homologene: 2042
Etl4
Name: enhancer trap locus 4
Synonyms: Sickle tail, Etl-4, Skt, E330027G05Rik, 6620402G01Rik, 9430077C05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 208618
Homologene: 10477
Mon2
Name: MON2 homolog, regulator of endosome to Golgi trafficking
Synonyms: 2610528O22Rik, SF21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67074
VEGA: 10
Homologene: 44309
Sucla2
Name: succinate-Coenzyme A ligase, ADP-forming, beta subunit
Synonyms: 4930547K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 20916
Homologene: 2856
Txnrd1
Name: thioredoxin reductase 1
Synonyms: TR alpha, TrxR1, TR1, TR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 50493
VEGA: 10
Homologene: 55733
Kdm8
Name: lysine (K)-specific demethylase 8
Synonyms: 3110005O21Rik, Jmjd5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 77035
Homologene: 49791
Eef1aknmt
Name: EEF1A lysine and N-terminal methyltransferase
Synonyms: Mettl13, 5630401D24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71449
Homologene: 32285
Atp13a3
Name: ATPase type 13A3
Synonyms: LOC224088, LOC385637, LOC224087
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224088
Homologene: 23455
Plekhg1
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 1
Synonyms: D10Ertd733e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 213783
Homologene: 18897
H13
Name: histocompatibility 13
Synonyms: 5031424B04Rik, 1200006O09Rik, 4930443L17Rik, Hm13, H-13, Spp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14950
Homologene: 7749
Lmo2
Name: LIM domain only 2
Synonyms: Rbtn-2, Rbtn2, Rhom-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16909
HGNC: HGNC:6642
Homologene: 4072
Qrfprl
Name: pyroglutamylated RFamide peptide receptor like
Synonyms: C130060K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243407
Homologene: 135976
Aqp9
Name: aquaporin 9
Synonyms: 1700020I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 64008
HGNC: HGNC:643
Homologene: 41405
Adam15
Name: a disintegrin and metallopeptidase domain 15 (metargidin)
Synonyms: metargidin, MDC15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 11490
HGNC: HGNC:193
Homologene: 2829
Pkd2l2
Name: polycystic kidney disease 2-like 2
Synonyms: TRPP5, Polycystin - L2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 53871
VEGA: 18
HGNC: HGNC:9012
Homologene: 22812
Siglec1
Name: sialic acid binding Ig-like lectin 1, sialoadhesin
Synonyms: CD169, Siglec-1, Sn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20612
Homologene: 124458
Svep1
Name: sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1
Synonyms: D430029O09Rik, 4833413O10Rik, 1110021D17Rik, Polydom
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 64817
Homologene: 23386
Slc1a5
Name: solute carrier family 1 (neutral amino acid transporter), member 5
Synonyms: ASCT2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20514
Homologene: 21155
Spta1
Name: spectrin alpha, erythrocytic 1
Synonyms: Spna-1, erythroid, Spna1, ihj
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20739
Homologene: 74460
Rin2
Name: Ras and Rab interactor 2
Synonyms: RASSF4, 2010003K16Rik, 4632403N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74030
Homologene: 32430
B4galt3
Name: UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3
Synonyms: beta4GalT-III, ESTM26, R74981, 9530061M23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 57370
HGNC: HGNC:926
Homologene: 48241
Vmn2r15
Name: vomeronasal 2, receptor 15
Synonyms: EG211223
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 211223
Homologene: 129606
Spag16
Name: sperm associated antigen 16
Synonyms: Wdr29, 4930524F24Rik, 4930585K05Rik, 4921511D23Rik, Pf20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66722
Homologene: 11572
Gys1
Name: glycogen synthase 1, muscle
Synonyms: MGS, Gys3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14936
HGNC: HGNC:4706
Homologene: 113557
Nup210
Name: nucleoporin 210
Synonyms: gp190, gp210, Pom210
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54563
Homologene: 41286
Megf6
Name: multiple EGF-like-domains 6
Synonyms: 2600001P17Rik, Egfl3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230971
HGNC: HGNC:3232
Homologene: 45412
Igfn1
Name: immunoglobulin-like and fibronectin type III domain containing 1
Synonyms: 9830123M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226438
Homologene: 130054
Lbp
Name: lipopolysaccharide binding protein
Synonyms: Bpifd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16803
HGNC: HGNC:6517
Homologene: 3055
AC149051.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Rhbdd1
Name: rhomboid domain containing 1
Synonyms: 4930418P06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 76867
Homologene: 12659
Olfr1328
Name: olfactory receptor 1328
Synonyms: Olfr1519, MOR259-1, MOR259-13, MOR259-1, GA_x6K02T2QD9B-18602750-18603691
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 258394
Homologene: 121524
Usp45
Name: ubiquitin specific petidase 45
Synonyms: 3110003C05Rik, Gcap7, 4930550B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 77593
Homologene: 17674
Olfr1031
Name: olfactory receptor 1031
Synonyms: GA_x6K02T2Q125-47470765-47471775, MOR200-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 257916
Homologene: 73973
Opcml
Name: opioid binding protein/cell adhesion molecule-like
Synonyms: B930023M13Rik, 2900075O15Rik, LOC235104, Obcam
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 330908
HGNC: HGNC:8143
Homologene: 55663
Pde3a
Name: phosphodiesterase 3A, cGMP inhibited
Synonyms: A930022O17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54611
HGNC: HGNC:8778
Homologene: 708
Vmn2r76
Name: vomeronasal 2, receptor 76
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 675969
Homologene: 104330
Dync1i1
Name: dynein cytoplasmic 1 intermediate chain 1
Synonyms: IC74, DIC, Dncic1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 13426
HGNC: HGNC:2963
Homologene: 68398
4930453N24Rik
Name: RIKEN cDNA 4930453N24 gene
Synonyms: din
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 67609
Homologene: 27867
Nkx3-2
Name: NK3 homeobox 2
Synonyms: Bapx1, Nkx-3.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12020
HGNC: HGNC:951
Homologene: 68168
AC123821.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Tspan8
Name: tetraspanin 8
Synonyms: E330007O21Rik, Tm4sf3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216350
VEGA: 10
Homologene: 20968
Mitd1
Name: MIT, microtubule interacting and transport, domain containing 1
Synonyms: 1500032H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 69028
Homologene: 14787
Cldn34c4
Name: claudin 34C4
Synonyms: 4930412D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 73934
Homologene: 132184
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 37,881,026 bp
  • A to T, chromosome 1 at 69,853,328 bp
  • A to G, chromosome 1 at 82,370,381 bp
  • A to G, chromosome 1 at 135,969,756 bp
  • A to G, chromosome 1 at 153,690,996 bp
  • G to T, chromosome 1 at 162,546,186 bp
  • C to A, chromosome 1 at 171,274,043 bp
  • T to C, chromosome 1 at 174,241,137 bp
  • T to A, chromosome 2 at 20,806,019 bp
  • T to A, chromosome 2 at 85,992,232 bp
  • T to C, chromosome 2 at 103,981,062 bp
  • A to G, chromosome 2 at 131,083,554 bp
  • C to T, chromosome 2 at 145,860,446 bp
  • C to G, chromosome 2 at 152,691,874 bp
  • T to A, chromosome 2 at 158,324,630 bp
  • A to G, chromosome 3 at 89,347,055 bp
  • T to C, chromosome 4 at 21,810,746 bp
  • A to C, chromosome 4 at 58,145,149 bp
  • T to C, chromosome 4 at 118,934,683 bp
  • T to C, chromosome 4 at 154,242,532 bp
  • T to A, chromosome 5 at 41,762,063 bp
  • T to A, chromosome 5 at 109,293,446 bp
  • A to G, chromosome 6 at 5,769,764 bp
  • T to A, chromosome 6 at 65,381,541 bp
  • C to T, chromosome 6 at 86,757,818 bp
  • A to T, chromosome 6 at 91,060,620 bp
  • T to C, chromosome 6 at 141,471,171 bp
  • T to C, chromosome 7 at 16,795,853 bp
  • T to A, chromosome 7 at 31,375,990 bp
  • A to G, chromosome 7 at 45,448,386 bp
  • A to C, chromosome 7 at 86,230,300 bp
  • T to A, chromosome 7 at 125,456,494 bp
  • A to T, chromosome 8 at 63,926,947 bp
  • A to G, chromosome 9 at 28,901,588 bp
  • C to A, chromosome 9 at 71,130,444 bp
  • A to G, chromosome 10 at 3,957,087 bp
  • G to A, chromosome 10 at 45,158,371 bp
  • A to G, chromosome 10 at 82,885,280 bp
  • C to T, chromosome 10 at 115,835,282 bp
  • A to G, chromosome 10 at 123,002,819 bp
  • T to C, chromosome 11 at 8,492,275 bp
  • T to A, chromosome 11 at 77,549,438 bp
  • A to G, chromosome 14 at 73,581,664 bp
  • C to A, chromosome 16 at 30,354,246 bp
  • A to G, chromosome 16 at 64,766,458 bp
  • T to C, chromosome 18 at 34,428,192 bp
  • C to A, chromosome X at 127,721,437 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4058 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040969-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.