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Strain Name:
C57BL/6J-MtgxR4059Btlr/Mmmh
Stock Number:
040970-MU
Citation ID:
RRID:MMRRC_040970-MU
Other Names:
R4059 (G1), C57BL/6J-MtgxR4059Btlr
Major Collection:

Strain Information

Rgs8
Name: regulator of G-protein signaling 8
Synonyms: 6530413N01Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67792
Homologene: 23152
Rnd3
Name: Rho family GTPase 3
Synonyms: 2610017M01Rik, Arhe, Rhoe
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74194
HGNC: HGNC:671
Homologene: 21074
Runx1
Name: runt related transcription factor 1
Synonyms: AML1, Pebp2a2, runt domain, alpha subunit 2, Cbfa2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12394
Homologene: 1331
Ntrk1
Name: neurotrophic tyrosine kinase, receptor, type 1
Synonyms: TrkA, Tkr
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18211
HGNC: HGNC:8031
Homologene: 1898
Dgat1
Name: diacylglycerol O-acyltransferase 1
Synonyms: D15Ertd23e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 13350
HGNC: HGNC:2843
Homologene: 7688
Ptcd2
Name: pentatricopeptide repeat domain 2
Synonyms: 1190005P08Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 68927
VEGA: 13
Homologene: 11695
Aspscr1
Name: ASPSCR1 tether for SLC2A4, UBX domain containing
Synonyms: ASPCR1, RCC17, ASPC, 1190006K01Rik, TUG, ASPL
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68938
Homologene: 41550
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 87,486,231 bp
  • A to G, chromosome 1 at 135,969,756 bp
  • A to G, chromosome 1 at 153,690,996 bp
  • G to T, chromosome 1 at 162,546,186 bp
  • C to A, chromosome 1 at 171,274,043 bp
  • G to T, chromosome 1 at 172,538,489 bp
  • A to G, chromosome 2 at 51,148,748 bp
  • C to T, chromosome 2 at 88,756,451 bp
  • T to C, chromosome 2 at 101,895,154 bp
  • T to C, chromosome 2 at 102,450,721 bp
  • T to C, chromosome 2 at 103,981,062 bp
  • T to C, chromosome 2 at 156,139,716 bp
  • A to G, chromosome 3 at 87,781,479 bp
  • A to G, chromosome 3 at 88,381,008 bp
  • T to C, chromosome 3 at 104,825,111 bp
  • T to C, chromosome 4 at 13,889,769 bp
  • T to C, chromosome 4 at 24,792,781 bp
  • C to T, chromosome 4 at 57,024,337 bp
  • G to A, chromosome 5 at 65,892,588 bp
  • T to C, chromosome 5 at 137,436,820 bp
  • T to G, chromosome 6 at 4,756,427 bp
  • T to C, chromosome 6 at 34,846,899 bp
  • A to C, chromosome 7 at 39,535,399 bp
  • T to C, chromosome 7 at 43,301,731 bp
  • T to C, chromosome 7 at 100,482,664 bp
  • C to T, chromosome 7 at 109,717,995 bp
  • T to C, chromosome 7 at 127,801,545 bp
  • G to T, chromosome 7 at 143,065,293 bp
  • A to G, chromosome 8 at 13,320,848 bp
  • A to G, chromosome 8 at 105,427,796 bp
  • A to G, chromosome 9 at 64,911,892 bp
  • A to G, chromosome 10 at 3,957,087 bp
  • T to C, chromosome 10 at 59,083,533 bp
  • A to G, chromosome 10 at 62,956,989 bp
  • T to C, chromosome 10 at 70,554,508 bp
  • A to G, chromosome 10 at 82,885,280 bp
  • A to G, chromosome 10 at 91,162,261 bp
  • C to T, chromosome 10 at 115,835,282 bp
  • A to G, chromosome 11 at 54,911,569 bp
  • A to G, chromosome 11 at 73,112,134 bp
  • C to T, chromosome 11 at 80,346,815 bp
  • T to C, chromosome 11 at 99,485,788 bp
  • C to T, chromosome 11 at 115,356,739 bp
  • T to A, chromosome 11 at 117,149,198 bp
  • T to C, chromosome 11 at 120,686,679 bp
  • T to C, chromosome 13 at 19,141,998 bp
  • A to G, chromosome 13 at 99,344,576 bp
  • A to G, chromosome 14 at 52,314,571 bp
  • C to T, chromosome 15 at 44,550,760 bp
  • G to T, chromosome 15 at 76,504,171 bp
  • C to A, chromosome 16 at 30,354,246 bp
  • A to G, chromosome 16 at 64,766,458 bp
  • A to G, chromosome 16 at 92,644,246 bp
  • A to G, chromosome 17 at 18,598,077 bp
  • G to A, chromosome 18 at 24,679,390 bp
  • A to G, chromosome 18 at 67,545,713 bp
  • T to A, chromosome 19 at 7,879,608 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4059 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040970-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.