Strain Name:
C57BL/6J-MtgxR4200Btlr/Mmmh
Stock Number:
041030-MU
Citation ID:
RRID:MMRRC_041030-MU
Other Names:
R4200 (G1), C57BL/6J-MtgxR4200Btlr
Major Collection:

Gene Information

Pknox1
Name: Pbx/knotted 1 homeobox
Synonyms: D17Wsu76e, PREP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18771
HGNC: HGNC:9022
Homologene: 3363
Olr1
Name: oxidized low density lipoprotein (lectin-like) receptor 1
Synonyms: Scare1, LOX-1, SR-EI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 108078
HGNC: HGNC:8133
Homologene: 1910
Ifi203
Name: interferon activated gene 203
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 15950
HGNC: HGNC:5395
Homologene: 115929
Xpnpep1
Name: X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
Synonyms: D230045I08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 170750
Homologene: 6424
Nup210l
Name: nucleoporin 210-like
Synonyms: 4930548O11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 77595
Homologene: 28122
Etl4
Name: enhancer trap locus 4
Synonyms: 6620402G01Rik, E330027G05Rik, Etl-4, 9430077C05Rik, Sickle tail, Skt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 208618
Homologene: 10477
Asxl1
Name: additional sex combs like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228790
Homologene: 9098
Zfp26
Name: zinc finger protein 26
Synonyms: 5033428C05Rik, Zfp70, Zfp81-rs1, KRAB15, mkr-3, Zfp-26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 22688
Homologene: 52318
Map2
Name: microtubule-associated protein 2
Synonyms: G1-397-34, Mtap2, MAP-2, repro4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17756
HGNC: HGNC:6839
Homologene: 1779
Trim29
Name: tripartite motif-containing 29
Synonyms: 2810431N19Rik, 4732461M22Rik, 1110047J21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72169
VEGA: 9
Homologene: 8100
Slc1a1
Name: solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
Synonyms: EAAC1, MEAAC1, EAAT3, D130048G10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20510
Homologene: 20881
Dnah3
Name: dynein, axonemal, heavy chain 3
Synonyms: Dnahc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381917
HGNC: HGNC:2949
Homologene: 19674
Vmn2r100
Name: vomeronasal 2, receptor 100
Synonyms: EG627537
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 627537
Homologene: 129750
Mboat7
Name: membrane bound O-acyltransferase domain containing 7
Synonyms: mBB1, Leng4, 5730589L02Rik, Lpiat1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 77582
Homologene: 11510
C7
Name: complement component 7
Synonyms: LOC383055
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 109828
VEGA: 15
HGNC: HGNC:1346
Homologene: 489
Col6a3
Name: collagen, type VI, alpha 3
Synonyms: Col6a-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12835
HGNC: HGNC:2213
Homologene: 37917
Mroh8
Name: maestro heat-like repeat family member 8
Synonyms: 4922505G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 629499
Homologene: 51864
Muc5b
Name: mucin 5, subtype B, tracheobronchial
Synonyms: MUC9, MUC5, 2300002I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74180
HGNC: HGNC:7516
Homologene: 136756
Aox3
Name: aldehyde oxidase 3
Synonyms: 1200011D03Rik, AOH1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71724
Homologene: 90899
Mest
Name: mesoderm specific transcript
Synonyms: Peg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17294
HGNC: HGNC:7028
Homologene: 1800
Psg23
Name: pregnancy-specific glycoprotein 23
Synonyms: 1620401C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56868
Homologene: 110989
Hnf4g
Name: hepatocyte nuclear factor 4, gamma
Synonyms: NR2A2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 30942
HGNC: HGNC:5026
Homologene: 37886
Ttc24
Name: tetratricopeptide repeat domain 24
Synonyms: A430025D11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 214191
Homologene: 82282
Olfr1357
Name: olfactory receptor 1357
Synonyms: MOR128-3, GA_x6K02T2QGN0-3196801-3197742, MOR128-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 257883
HGNC: HGNC:8207
Homologene: 72920
Foxg1
Name: forkhead box G1
Synonyms: Hfh9, Bf1, Hfhbf1, BF-1, 2900064B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 15228
HGNC: HGNC:3811
Homologene: 3843
Arhgap31
Name: Rho GTPase activating protein 31
Synonyms: CdGAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12549
Homologene: 10644
Aadac
Name: arylacetamide deacetylase
Synonyms: Aada, 5033417E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67758
HGNC: HGNC:17
Homologene: 37436
Dus2
Name: dihydrouridine synthase 2
Synonyms: Dus2l, 2310016K04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66369
Homologene: 6838
Ttll12
Name: tubulin tyrosine ligase-like family, member 12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223723
Homologene: 9036
Hyou1
Name: hypoxia up-regulated 1
Synonyms: Grp170, Orp150, CBP-140, Cab140, 140 kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12282
Homologene: 4658
Nfatc4
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 4
Synonyms: 3110041H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 73181
VEGA: 14
HGNC: HGNC:7778
Homologene: 3349
Olfr573-ps1
Name: olfactory receptor 573, pseudogene 1
Synonyms: MOR10-3P, GA_x6K02T2PBJ9-5653743-5652872, MOR10-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258230
Zfp58
Name: zinc finger protein 58
Synonyms: A530094I17Rik, Zfp817, Mfg-1, Mfg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238693
Tgm2
Name: transglutaminase 2, C polypeptide
Synonyms: G[a]h, tissue transglutaminase, TG2, TGase2, tTGas, TG C, protein-glutamine gamma-glutamyltransferase, tTG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21817
Homologene: 3391
Ttll1
Name: tubulin tyrosine ligase-like 1
Synonyms: 6330444E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 319953
HGNC: HGNC:1312
Homologene: 8174
Vmn1r18
Name: vomeronasal 1 receptor 18
Synonyms: V1rc26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 171199
Homologene: 138094
Gm3336
Name: predicted gene 3336
Synonyms: 2410018E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 100502950
Homologene: 136417
Pcdhgb5
Name: protocadherin gamma subfamily B, 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93702
HGNC: HGNC:8712
Homologene: 55773
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 58,188,378 bp
  • C to T, chromosome 1 at 66,425,298 bp
  • C to A, chromosome 1 at 90,801,383 bp
  • A to C, chromosome 1 at 173,924,115 bp
  • C to A, chromosome 2 at 20,781,883 bp
  • C to A, chromosome 2 at 153,400,106 bp
  • C to T, chromosome 2 at 157,241,810 bp
  • C to A, chromosome 2 at 158,132,490 bp
  • T to G, chromosome 3 at 3,651,284 bp
  • T to A, chromosome 3 at 60,039,534 bp
  • T to C, chromosome 3 at 88,074,517 bp
  • T to C, chromosome 3 at 90,119,911 bp
  • T to C, chromosome 6 at 30,742,187 bp
  • A to T, chromosome 6 at 57,390,116 bp
  • A to G, chromosome 6 at 129,502,105 bp
  • C to A, chromosome 7 at 3,685,753 bp
  • T to C, chromosome 7 at 18,612,065 bp
  • A to G, chromosome 7 at 102,941,797 bp
  • C to T, chromosome 7 at 119,922,838 bp
  • T to A, chromosome 7 at 141,858,925 bp
  • A to T, chromosome 8 at 70,720,612 bp
  • A to G, chromosome 8 at 106,044,813 bp
  • T to C, chromosome 9 at 20,436,716 bp
  • G to T, chromosome 9 at 43,311,380 bp
  • G to A, chromosome 9 at 44,388,859 bp
  • A to T, chromosome 10 at 78,612,067 bp
  • T to A, chromosome 12 at 49,385,299 bp
  • T to G, chromosome 13 at 67,491,321 bp
  • C to T, chromosome 14 at 55,832,032 bp
  • A to G, chromosome 15 at 4,990,309 bp
  • A to T, chromosome 15 at 83,492,577 bp
  • T to C, chromosome 15 at 83,577,013 bp
  • G to A, chromosome 16 at 38,623,913 bp
  • T to A, chromosome 17 at 19,522,535 bp
  • C to A, chromosome 17 at 31,599,610 bp
  • A to G, chromosome 18 at 37,731,982 bp
  • A to G, chromosome 19 at 28,901,452 bp
  • G to A, chromosome 19 at 52,996,973 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4200 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041030-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.