Within sex and replication, the prone lines clearly display high withdrawal-convulsion scores.
The WSP-2 and WSR-2 mice also did not appear to differ systematically on BEC in early generations. Female and male WSP-2 mice reached higher Blood Ethanol Concentrations (BECs) than female and male WSR-2 mice in S0 (26 and 69%, respectively), but no differences were seen in S11.
Withdrawal Seizure-Prone, replicate 2 (WSP2) mice were selectively bred for high alcohol withdrawal convulsion severity after 72hr of continuous chronic ethanol vapor inhalation. The founder stock for selective breeding was the HS/Ibg heterogeneous stock that was developed by Gerald McClearn and colleagues (The Institute for Behavioral Genetics, Boulder, Colorado) as an 8-way cross of the following inbred strains obtained from the Cancer Research Genetics Laboratory at UC-Berkeley: A, AKR, BALB/c, C3H/2, C57BL, DBA/2, Is/Bi, and RIII.
Eighteen matings of Generation 30 HS/Ibg mice were randomly divided into 2 groups of 9 families each. These generation 31 mice became the foundation (So) population for selection based on alcohol withdrawal severity. One group of 9 familes was designated Replicate 1, the other Replicate 2. To establish the Withdrawal Seizure-Prone Replicate 2 (WSP2) line the highest scoring male and female from each family were selected. Selected progeny from each family were then mated using a rotational system – female from Family 1 mated to male from Family 2, female from Family 2 mated to male from Family 3, etc. Two to six reserve families were generated to provide back up. The selection process was repeated for 26 generations. Relaxed breeding (random selection of breeders from within each family) has continued for approximately 100 generations. The MMRRC imported a combination of the 9 families to form the WSP2 line in 2016.
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Yes||Yes|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||4 to 6 months||4 to 6 months|
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