Strain Name:
C57BL/6J-MtgxR4526Btlr/Mmmh
Stock Number:
041591-MU
Citation ID:
RRID:MMRRC_041591-MU
Other Names:
R4526 (G1), C57BL/6J-MtgxR4526Btlr
Major Collection:

Gene Information

Zmynd8
Name: zinc finger, MYND-type containing 8
Synonyms: Prkcbp1, ZMYND8, RACK7, 3632413B07Rik, 1110013E22Rik, 2010005I16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228880
HGNC: HGNC:9397
Homologene: 32679
Plekhm1
Name: pleckstrin homology domain containing, family M (with RUN domain) member 1
Synonyms: D330036J23Rik, AP162, B2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 353047
Homologene: 8871
Usp5
Name: ubiquitin specific peptidase 5 (isopeptidase T)
Synonyms: Ucht
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 22225
Homologene: 55758
Myh10
Name: myosin, heavy polypeptide 10, non-muscle
Synonyms: SMemb, nonmuscle myosin heavy chain IIB, Fltn, NMHC II-B, NMHC-B, Myosin IIB, nonmuscle myosin heavy chain II-B, Fltn, 9330167F11Rik, Myhn2, 5730504C04Rik, Myhn-2, myosin IIB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 77579
HGNC: HGNC:7568
Homologene: 55941
Rbm25
Name: RNA binding motif protein 25
Synonyms: 2610015J01Rik, A130095G20Rik, 2600011C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 67039
Snrnp200
Name: small nuclear ribonucleoprotein 200 (U5)
Synonyms: HELIC2, A330064G03Rik, Ascc3l1, U5-200KD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 320632
Homologene: 5859
Nek1
Name: NIMA (never in mitosis gene a)-related expressed kinase 1
Synonyms: kat, kidney, anemia and testis, D8Ertd790e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 18004
HGNC: HGNC:7744
Homologene: 14376
Rims2
Name: regulating synaptic membrane exocytosis 2
Synonyms: 2810036I15Rik, RIM2, Syt3-rs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 116838
VEGA: 15
Homologene: 81639
Zfp318
Name: zinc finger protein 318
Synonyms: D530032D06Rik, 2610034E08Rik, TZF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 57908
Homologene: 22808
Polr2b
Name: polymerase (RNA) II (DNA directed) polypeptide B
Synonyms: RPB2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231329
HGNC: HGNC:9188
Homologene: 722
Scamp4
Name: secretory carrier membrane protein 4
Synonyms: Sc4, 2410022D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 56214
VEGA: 10
Homologene: 10476
Cbwd1
Name: COBW domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 226043
VEGA: 19
Homologene: 6843
Plvap
Name: plasmalemma vesicle associated protein
Synonyms: MECA32, PV-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 84094
Homologene: 10578
Arl13b
Name: ADP-ribosylation factor-like 13B
Synonyms: A930014M17Rik, C530009C10Rik, Arl2l1, hnn, A530097K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 68146
Homologene: 18820
Dnah1
Name: dynein, axonemal, heavy chain 1
Synonyms: B230373P09Rik, E030034C22Rik, Dnahc1, MDHC7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 110084
VEGA: 14
HGNC: HGNC:2940
Homologene: 67131
Ptpn13
Name: protein tyrosine phosphatase, non-receptor type 13
Synonyms: PTPL1, PTP-BL, Ptpri
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 19249
HGNC: HGNC:9646
Homologene: 7909
Mapk15
Name: mitogen-activated protein kinase 15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 332110
Homologene: 16371
Vmn2r6
Name: vomeronasal 2, receptor 6
Synonyms: EG620718, EG667069
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 667069
Homologene: 129754
D430041D05Rik
Name: RIKEN cDNA D430041D05 gene
Synonyms: G2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241589
Homologene: 115928
Ly75
Name: lymphocyte antigen 75
Synonyms: CD205, DEC-205
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 17076
HGNC: HGNC:6729
Homologene: 31085
Ssu2
Name: ssu-2 homolog (C. elegans)
Synonyms: D630042P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 243612
Homologene: 136556
Adgre4
Name: adhesion G protein-coupled receptor E4
Synonyms: EGF-TM7, FIRE, Gpr127, Emr4, D17Ertd479e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 52614
VEGA: 17
Homologene: 64640
Stard7
Name: START domain containing 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 99138
Homologene: 32463
Otogl
Name: otogelin-like
Synonyms: Gm6924
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 628870
Homologene: 46008
Gli3
Name: GLI-Kruppel family member GLI3
Synonyms: Bph, brachyphalangy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 14634
HGNC: HGNC:4319
Homologene: 139
Olfr1098
Name: olfactory receptor 1098
Synonyms: MOR206-1, GA_x6K02T2Q125-48410458-48409511
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258842
Homologene: 37005
Lman2l
Name: lectin, mannose-binding 2-like
Synonyms: VIP36-like, A630028F14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 214895
Homologene: 57125
Olfr1156
Name: olfactory receptor 1156
Synonyms: GA_x6K02T2Q125-49433499-49432537, MOR174-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258814
HGNC: HGNC:8351
Homologene: 72031
Vmn2r60
Name: vomeronasal 2, receptor 60
Synonyms: Casr-rs3, EG637898, Gprc2a-rs3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 637898
Homologene: 129683
4930474N05Rik
Name: RIKEN cDNA 4930474N05 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 218921
Homologene: 138428
Olfr1269
Name: olfactory receptor 1269
Synonyms: MOR228-3, GA_x6K02T2Q125-51551618-51550689
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258339
Homologene: 133727
Crebzf
Name: CREB/ATF bZIP transcription factor
Synonyms: LAZipII, Zf, 1110034C16Rik, LAZip, 6330417B10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233490
Homologene: 10926
Lcn9
Name: lipocalin 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 77704
Homologene: 12681
Oas1b
Name: 2'-5' oligoadenylate synthetase 1B
Synonyms: Mmu-L1, Oias-2, L1, Wnv, Flavivirus resistance, Flv, Oias2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 23961
HGNC: HGNC:8086
Gm17542
Name: predicted gene, 17542
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
Gm26561
Name: predicted gene, 26561
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 36,438,763 bp
  • A to C, chromosome 2 at 25,824,508 bp
  • G to A, chromosome 2 at 60,330,773 bp
  • C to T, chromosome 2 at 86,922,995 bp
  • T to C, chromosome 2 at 87,949,409 bp
  • T to C, chromosome 2 at 90,118,672 bp
  • A to G, chromosome 2 at 104,192,433 bp
  • A to G, chromosome 2 at 127,229,102 bp
  • A to G, chromosome 2 at 127,297,208 bp
  • TTGCTGCTGCTGCTGCTG to TTGCTGCTGCTGCTG, chromosome 2 at 165,807,607 bp
  • A to G, chromosome 3 at 64,537,724 bp
  • T to G, chromosome 5 at 77,326,714 bp
  • T to C, chromosome 5 at 103,501,469 bp
  • T to A, chromosome 5 at 120,822,102 bp
  • A to G, chromosome 6 at 112,382,422 bp
  • C to G, chromosome 6 at 124,822,630 bp
  • A to G, chromosome 7 at 42,195,243 bp
  • A to C, chromosome 7 at 90,443,760 bp
  • T to C, chromosome 8 at 61,106,944 bp
  • G to A, chromosome 8 at 71,507,771 bp
  • T to C, chromosome 10 at 58,713,613 bp
  • T to C, chromosome 10 at 80,611,057 bp
  • G to A, chromosome 10 at 107,886,980 bp
  • C to T, chromosome 11 at 68,815,049 bp
  • A to G, chromosome 11 at 103,395,304 bp
  • C to A, chromosome 12 at 83,664,222 bp
  • A to G, chromosome 13 at 15,713,631 bp
  • C to A, chromosome 14 at 31,285,998 bp
  • C to T, chromosome 14 at 36,096,578 bp
  • A to T, chromosome 15 at 39,437,717 bp
  • A to T, chromosome 15 at 75,995,255 bp
  • T to C, chromosome 16 at 62,812,011 bp
  • T to A, chromosome 17 at 46,412,358 bp
  • C to A, chromosome 17 at 55,785,016 bp
  • T to A, chromosome 17 at 71,237,378 bp
  • A to G, chromosome 19 at 24,957,964 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4526 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041591-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.