Strain Name:
C57BL/6J-MtgxR4474Btlr/Mmmh
Stock Number:
041731-MU
Citation ID:
RRID:MMRRC_041731-MU
Other Names:
R4474 (G1), C57BL/6J-MtgxR4474Btlr
Major Collection:

Gene Information

Cdk19
Name: cyclin-dependent kinase 19
Synonyms: Cdc2l6, 2700084L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 78334
VEGA: 10
Homologene: 22862
Mov10
Name: Mov10 RISC complex RNA helicase
Synonyms: Mov-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 17454
HGNC: HGNC:7200
Homologene: 10365
Cep104
Name: centrosomal protein 104
Synonyms: BC046331
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230967
Homologene: 44919
Parn
Name: poly(A)-specific ribonuclease (deadenylation nuclease)
Synonyms: DAN, 1200003I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74108
VEGA: 16
HGNC: HGNC:8609
Homologene: 31098
Siah1b
Name: siah E3 ubiquitin protein ligase 1B
Synonyms: Sinh1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 20438
Homologene: 56441
Rims2
Name: regulating synaptic membrane exocytosis 2
Synonyms: Syt3-rs, 2810036I15Rik, RIM2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 116838
VEGA: 15
Homologene: 81639
Mdh1
Name: malate dehydrogenase 1, NAD (soluble)
Synonyms: MDH-s, B230377B03Rik, Mor2, Mor-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17449
HGNC: HGNC:6970
Homologene: 4324
Sppl3
Name: signal peptide peptidase 3
Synonyms: 4833416I09Rik, Usmg3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 74585
Homologene: 15563
Rab22a
Name: RAB22A, member RAS oncogene family
Synonyms: Rab22, 3732413A17Rik, E130120E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19334
HGNC: HGNC:9764
Homologene: 10782
Kif13a
Name: kinesin family member 13A
Synonyms: 4930505I07Rik, N-3 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 16553
VEGA: 13
Homologene: 22589
Kcna4
Name: potassium voltage-gated channel, shaker-related subfamily, member 4
Synonyms: Kv1.4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16492
HGNC: HGNC:6222
Homologene: 20514
Cdh23
Name: cadherin 23 (otocadherin)
Synonyms: mdfw, USH1D, ahl, nmf252, nmf181, nmf112, sals, bob, 4930542A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22295
Homologene: 11142
Tsc2
Name: TSC complex subunit 2
Synonyms: tuberous sclerosis 2, Nafld, tuberin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22084
VEGA: 17
Homologene: 462
Hydin
Name: HYDIN, axonemal central pair apparatus protein
Synonyms: hyrh, hy3, hy-3, 1700034M11Rik, 4930545D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244653
Homologene: 52118
Pik3c2g
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 gamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 18705
HGNC: HGNC:8973
Homologene: 3362
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: Schnurri-2, Gm20114, Shn-2, MIBP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Dclre1b
Name: DNA cross-link repair 1B
Synonyms: Apollo, SNMIB, mSNM1B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 140917
Homologene: 32553
Csf1
Name: colony stimulating factor 1 (macrophage)
Synonyms: CSF-1, M-CSF, colony-stimulating factor-1, Csfm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12977
HGNC: HGNC:2432
Homologene: 7282
Sarm1
Name: sterile alpha and HEAT/Armadillo motif containing 1
Synonyms: A830091I15Rik, MyD88-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237868
Homologene: 9015
Acss1
Name: acyl-CoA synthetase short-chain family member 1
Synonyms: Acas2l, 1110032O15Rik, Acas2, AceCS2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68738
Homologene: 56037
Abca6
Name: ATP-binding cassette, sub-family A (ABC1), member 6
Synonyms: 6330565N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76184
HGNC: HGNC:36
Homologene: 71264
Lingo2
Name: leucine rich repeat and Ig domain containing 2
Synonyms: Lrrn6c, B230217C06Rik, LERN3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242384
Homologene: 17621
Olfr851
Name: olfactory receptor 851
Synonyms: GA_x6K02T2PVTD-13234278-13235216, MOR155-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258907
Homologene: 74178
Ugt1a6a
Name: UDP glucuronosyltransferase 1 family, polypeptide A6A
Synonyms: UGT1.6, Ugt1a6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 94284
Homologene: 85959
Erbb2
Name: erb-b2 receptor tyrosine kinase 2
Synonyms: Neu, ErbB-2, l11Jus8, c-erbB2, HER2, HER-2, c-neu, Neu oncogene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 13866
HGNC: HGNC:3430
Homologene: 3273
Gm9573
Name: predicted gene 9573
Synonyms: Muc21, epiglycanin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 672682
Fbp1
Name: fructose bisphosphatase 1
Synonyms: Fbp3, Fbp-2, FBPase brain isoform, FBPase liver
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 14121
HGNC: HGNC:3606
Homologene: 55467
Vmn2r95
Name: vomeronasal 2, receptor 95
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 328759
Homologene: 115024
Alpk1
Name: alpha-kinase 1
Synonyms: 8430410J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71481
Homologene: 11849
Rassf5
Name: Ras association (RalGDS/AF-6) domain family member 5
Synonyms: Rapl, Nore1B, 1300019G20Rik, Nore1A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 54354
Homologene: 10296
Rhot2
Name: ras homolog family member T2
Synonyms: Arht2, Miro2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 214952
Homologene: 56038
Snx31
Name: sorting nexin 31
Synonyms: 4631426E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 66696
Homologene: 23551
Olfr1305
Name: olfactory receptor 1305
Synonyms: GA_x6K02T2Q125-72930843-72929905, MOR245-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258396
Homologene: 74056
Zfp36l3
Name: zinc finger protein 36, C3H type-like 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 333473
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • TAAAAAAAAA to TAAAAAAA, chromosome 1 at 88,215,928 bp
  • G to A, chromosome 1 at 131,212,338 bp
  • A to G, chromosome 2 at 107,296,007 bp
  • G to A, chromosome 2 at 111,873,439 bp
  • G to A, chromosome 2 at 150,637,913 bp
  • C to T, chromosome 2 at 173,695,263 bp
  • C to A, chromosome 3 at 103,807,243 bp
  • C to T, chromosome 3 at 104,818,465 bp
  • T to C, chromosome 3 at 107,753,856 bp
  • T to C, chromosome 3 at 127,680,018 bp
  • T to C, chromosome 4 at 35,708,810 bp
  • T to A, chromosome 4 at 153,989,236 bp
  • G to A, chromosome 5 at 115,088,294 bp
  • T to A, chromosome 6 at 139,633,751 bp
  • G to T, chromosome 8 at 110,563,865 bp
  • T to A, chromosome 9 at 19,496,877 bp
  • C to A, chromosome 10 at 14,128,969 bp
  • G to A, chromosome 10 at 40,469,956 bp
  • G to T, chromosome 10 at 60,311,086 bp
  • T to G, chromosome 11 at 21,566,624 bp
  • T to A, chromosome 11 at 78,497,101 bp
  • G to A, chromosome 11 at 98,425,179 bp
  • A to T, chromosome 11 at 110,233,772 bp
  • C to T, chromosome 13 at 46,814,155 bp
  • A to G, chromosome 13 at 62,875,261 bp
  • A to G, chromosome 15 at 36,546,111 bp
  • A to G, chromosome 15 at 39,462,560 bp
  • A to G, chromosome 16 at 13,664,685 bp
  • T to C, chromosome 17 at 18,452,245 bp
  • T to A, chromosome 17 at 24,597,264 bp
  • C to T, chromosome 17 at 25,841,382 bp
  • G to T, chromosome 17 at 35,620,604 bp
  • T to C, chromosome X at 53,775,957 bp
  • G to A, chromosome X at 164,071,692 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4474 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041731-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.