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Strain Name:
C57BL/6J-MtgxR4474Btlr/Mmmh
Stock Number:
041731-MU
Citation ID:
RRID:MMRRC_041731-MU
Other Names:
R4474 (G1), C57BL/6J-MtgxR4474Btlr
Major Collection:

Strain Information

Cdk19
Name: cyclin dependent kinase 19
Synonyms: 2700084L06Rik, Cdc2l6
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 78334
VEGA: 10
Homologene: 22862
Mov10
Name: Mov10 RISC complex RNA helicase
Synonyms: Mov-10
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 17454
HGNC: HGNC:7200
Homologene: 10365
Cep104
Name: centrosomal protein 104
Synonyms: BC046331
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230967
Homologene: 44919
Parn
Name: poly(A)-specific ribonuclease (deadenylation nuclease)
Synonyms: DAN, 1200003I18Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 74108
VEGA: 16
HGNC: HGNC:8609
Homologene: 31098
Siah1b
Name: siah E3 ubiquitin protein ligase 1B
Synonyms: Sinh1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 20438
Homologene: 56441
Rims2
Name: regulating synaptic membrane exocytosis 2
Synonyms: RIM2, 2810036I15Rik, Syt3-rs
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 116838
VEGA: 15
Homologene: 81639
Mdh1
Name: malate dehydrogenase 1, NAD (soluble)
Synonyms: Mor-2, MDH-s, Mor2, B230377B03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17449
HGNC: HGNC:6970
Homologene: 4324
Sppl3
Name: signal peptide peptidase 3
Synonyms: 4833416I09Rik, Usmg3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 74585
Homologene: 15563
Rab22a
Name: RAB22A, member RAS oncogene family
Synonyms: E130120E14Rik, 3732413A17Rik, Rab22
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19334
HGNC: HGNC:9764
Homologene: 10782
Kif13a
Name: kinesin family member 13A
Synonyms: N-3 kinesin, 4930505I07Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16553
VEGA: 13
Homologene: 22589
Kcna4
Name: potassium voltage-gated channel, shaker-related subfamily, member 4
Synonyms: Kv1.4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16492
HGNC: HGNC:6222
Homologene: 20514
Cdh23
Name: cadherin related 23 (otocadherin)
Synonyms: 4930542A03Rik, USH1D, mdfw, ahl, nmf252, bob, nmf112, nmf181, sals
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22295
Homologene: 11142
Tsc2
Name: TSC complex subunit 2
Synonyms: tuberin, Nafld, tuberous sclerosis 2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22084
VEGA: 17
Homologene: 462
Hydin
Name: HYDIN, axonemal central pair apparatus protein
Synonyms: hy-3, hy3, 1700034M11Rik, 4930545D19Rik, hyrh
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244653
Homologene: 52118
Pik3c2g
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 gamma
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 18705
HGNC: HGNC:8973
Homologene: 3362
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: MIBP1, Schnurri-2, Shn-2, Gm20114
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Dclre1b
Name: DNA cross-link repair 1B
Synonyms: mSNM1B, Apollo, SNMIB
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 140917
Homologene: 32553
Csf1
Name: colony stimulating factor 1 (macrophage)
Synonyms: CSF-1, M-CSF, colony-stimulating factor-1, Csfm, BAP025
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 12977
HGNC: HGNC:2432
Homologene: 7282
Sarm1
Name: sterile alpha and HEAT/Armadillo motif containing 1
Synonyms: MyD88-5, A830091I15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237868
Homologene: 9015
Acss1
Name: acyl-CoA synthetase short-chain family member 1
Synonyms: 1110032O15Rik, AceCS2, Acas2, Acas2l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68738
Homologene: 56037
Abca6
Name: ATP-binding cassette, sub-family A member 6
Synonyms: 6330565N06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76184
HGNC: HGNC:36
Homologene: 71264
Lingo2
Name: leucine rich repeat and Ig domain containing 2
Synonyms: B230217C06Rik, Lrrn6c, LERN3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242384
Homologene: 17621
Or7g32
Name: olfactory receptor family 7 subfamily G member 32
Synonyms: GA_x6K02T2PVTD-13234278-13235216, MOR155-1, Olfr851
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258907
HGNC: HGNC:8466
Homologene: 74178
Ugt1a6a
Name: UDP glucuronosyltransferase 1 family, polypeptide A6A
Synonyms: UGT1.6, Ugt1a6
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 94284
Homologene: 85959
Erbb2
Name: erb-b2 receptor tyrosine kinase 2
Synonyms: Neu, Neu oncogene, c-erbB2, HER-2, ErbB-2, HER2, c-neu, l11Jus8
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13866
HGNC: HGNC:3430
Homologene: 3273
Muc21
Name: mucin 21
Synonyms: epiglycanin, Gm9573
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 672682
Fbp1
Name: fructose bisphosphatase 1
Synonyms: FBPase brain isoform, FBPase liver, Fbp-2, Fbp3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 14121
HGNC: HGNC:3606
Homologene: 55467
Vmn2r95
Name: vomeronasal 2, receptor 95
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 328759
Homologene: 115024
Alpk1
Name: alpha-kinase 1
Synonyms: 8430410J10Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71481
Homologene: 11849
Rassf5
Name: Ras association (RalGDS/AF-6) domain family member 5
Synonyms: 1300019G20Rik, Nore1B, Nore1A, Rapl
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 54354
Homologene: 10296
Rhot2
Name: ras homolog family member T2
Synonyms: Miro2, Arht2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 214952
Homologene: 56038
Snx31
Name: sorting nexin 31
Synonyms: 4631426E05Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 66696
Homologene: 23551
Or4f56
Name: olfactory receptor family 4 subfamily F member 56
Synonyms: GA_x6K02T2Q125-72930843-72929905, MOR245-8, Olfr1305
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258396
Homologene: 74056
Zfp36l3
Name: zinc finger protein 36, C3H type-like 3
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 333473
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • TAAAAAAAAA to TAAAAAAA, chromosome 1 at 88,215,928 bp
  • G to A, chromosome 1 at 131,212,338 bp
  • A to G, chromosome 2 at 107,296,007 bp
  • G to A, chromosome 2 at 111,873,439 bp
  • G to A, chromosome 2 at 150,637,913 bp
  • C to T, chromosome 2 at 173,695,263 bp
  • C to A, chromosome 3 at 103,807,243 bp
  • C to T, chromosome 3 at 104,818,465 bp
  • T to C, chromosome 3 at 107,753,856 bp
  • T to C, chromosome 3 at 127,680,018 bp
  • T to C, chromosome 4 at 35,708,810 bp
  • T to A, chromosome 4 at 153,989,236 bp
  • G to A, chromosome 5 at 115,088,294 bp
  • T to A, chromosome 6 at 139,633,751 bp
  • G to T, chromosome 8 at 110,563,865 bp
  • T to A, chromosome 9 at 19,496,877 bp
  • C to A, chromosome 10 at 14,128,969 bp
  • G to A, chromosome 10 at 40,469,956 bp
  • G to T, chromosome 10 at 60,311,086 bp
  • T to G, chromosome 11 at 21,566,624 bp
  • T to A, chromosome 11 at 78,497,101 bp
  • G to A, chromosome 11 at 98,425,179 bp
  • A to T, chromosome 11 at 110,233,772 bp
  • C to T, chromosome 13 at 46,814,155 bp
  • A to G, chromosome 13 at 62,875,261 bp
  • A to G, chromosome 15 at 36,546,111 bp
  • A to G, chromosome 15 at 39,462,560 bp
  • A to G, chromosome 16 at 13,664,685 bp
  • T to C, chromosome 17 at 18,452,245 bp
  • T to A, chromosome 17 at 24,597,264 bp
  • C to T, chromosome 17 at 25,841,382 bp
  • G to T, chromosome 17 at 35,620,604 bp
  • T to C, chromosome X at 53,775,957 bp
  • G to A, chromosome X at 164,071,692 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4474 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041731-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.