Strain Name:
C57BL/6J-MtgxR4509Btlr/Mmmh
Stock Number:
041758-MU
Citation ID:
RRID:MMRRC_041758-MU
Other Names:
R4509 (G1), C57BL/6J-MtgxR4509Btlr
Major Collection:

Gene Information

Arid1a
Name: AT rich interactive domain 1A (SWI-like)
Synonyms: Osa1, 1110030E03Rik, Smarcf1, BAF250a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 93760
Homologene: 21216
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: ASC1p200, B630009I04Rik, Helic1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 77987
VEGA: 10
Homologene: 4973
Zfhx3
Name: zinc finger homeobox 3
Synonyms: WBP9, A230102L03Rik, Atbf1, Sci
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11906
HGNC: HGNC:777
Homologene: 21366
Etl4
Name: enhancer trap locus 4
Synonyms: Etl-4, E330027G05Rik, 6620402G01Rik, 9430077C05Rik, Skt, Sickle tail
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 208618
Homologene: 10477
Tdp1
Name: tyrosyl-DNA phosphodiesterase 1
Synonyms: 4921509N21Rik, SCAN1, 2810481F14Rik, E430034L06Rik, Gm40556
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 104884
Homologene: 5424
Ints9
Name: integrator complex subunit 9
Synonyms: D14Ertd231e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 210925
VEGA: 14
Homologene: 10096
Tnip1
Name: TNFAIP3 interacting protein 1
Synonyms: A20-binding inhibitor of NF-kappa B activation, ABIN1, VAN, Nef
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 57783
Homologene: 31355
Cttnbp2nl
Name: CTTNBP2 N-terminal like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 80281
Homologene: 36371
Mdn1
Name: midasin AAA ATPase 1
Synonyms: LOC213784, 4833432B22Rik, D4Abb1e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100019
Homologene: 39689
Ccdc77
Name: coiled-coil domain containing 77
Synonyms: 2400002C23Rik, 2700091N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67200
Homologene: 12096
Tnfrsf21
Name: tumor necrosis factor receptor superfamily, member 21
Synonyms: DR6, TR7, Death receptor 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 94185
VEGA: 17
Homologene: 8696
Vps13d
Name: vacuolar protein sorting 13D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230895
Homologene: 15583
Lca5
Name: Leber congenital amaurosis 5 (human)
Synonyms: 5730406O13Rik, ORF64, 4930431B11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 75782
Homologene: 32718
Pah
Name: phenylalanine hydroxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18478
HGNC: HGNC:8582
Homologene: 234
Lnx1
Name: ligand of numb-protein X 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 16924
HGNC: HGNC:6657
Homologene: 7819
Ppp3cb
Name: protein phosphatase 3, catalytic subunit, beta isoform
Synonyms: PP2BA beta, Calnb, CnAbeta, 1110063J16Rik, Cnab
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 19056
HGNC: HGNC:9315
Homologene: 56429
Atp5j
Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 11957
HGNC: HGNC:847
Homologene: 1272
Col1a2
Name: collagen, type I, alpha 2
Synonyms: Col1a-2, Cola2, Cola-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12843
HGNC: HGNC:2198
Homologene: 69
Rasgrp3
Name: RAS, guanyl releasing protein 3
Synonyms: LOC240168
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240168
VEGA: 17
Homologene: 15019
Camta2
Name: calmodulin binding transcription activator 2
Synonyms: Kiaa0909-hp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216874
Homologene: 9021
Itga4
Name: integrin alpha 4
Synonyms: VLA-4 receptor, alpha 4 subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16401
HGNC: HGNC:6140
Homologene: 37364
Muc6
Name: mucin 6, gastric
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 353328
HGNC: HGNC:7517
Homologene: 18768
Pik3c2g
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 gamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 18705
HGNC: HGNC:8973
Homologene: 3362
Zfp560
Name: zinc finger protein 560
Synonyms: 2310030G09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 434377
Homologene: 103820
Polq
Name: polymerase (DNA directed), theta
Synonyms: A430110D14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 77782
HGNC: HGNC:9186
Homologene: 32727
Cacna1d
Name: calcium channel, voltage-dependent, L type, alpha 1D subunit
Synonyms: D-LTCC, Cchl1a, Cchl1a2, Cacnl1a2, 8430418G19Rik, Cav1.3alpha1, C79217
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 12289
VEGA: 14
HGNC: HGNC:1391
Homologene: 578
Xpr1
Name: xenotropic and polytropic retrovirus receptor 1
Synonyms: suppressor of yeast Ga deletion, Syg1, Rmc-1, Rmc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19775
Homologene: 134226
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Slc44a5
Name: solute carrier family 44, member 5
Synonyms: LOC242259
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242259
Homologene: 72094
Perm1
Name: PPARGC1 and ESRR induced regulator, muscle 1
Synonyms: 2310042D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74183
Homologene: 135954
Abca8b
Name: ATP-binding cassette, sub-family A (ABC1), member 8b
Synonyms: Abca8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 27404
HGNC: HGNC:38
Homologene: 56029
Slc2a9
Name: solute carrier family 2 (facilitated glucose transporter), member 9
Synonyms: Glut9, SLC2A9B, SLC2a9A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 117591
Homologene: 69290
Ccdc178
Name: coiled coil domain containing 178
Synonyms: 4921528I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 70950
VEGA: 18
Homologene: 12373
Psd4
Name: pleckstrin and Sec7 domain containing 4
Synonyms: SEC7 homolog, EFA6B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 215632
Homologene: 8261
Adam6b
Name: a disintegrin and metallopeptidase domain 6B
Synonyms: 4930523C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238405
Homologene: 128362
Repin1
Name: replication initiator 1
Synonyms: Zfp464, AP4, E430037F08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 58887
Homologene: 22810
Gzmg
Name: granzyme G
Synonyms: Ctla7, Ctla-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 14944
VEGA: 14
HGNC: HGNC:4710
Homologene: 133275
Adam4
Name: a disintegrin and metallopeptidase domain 4
Synonyms: tMDCV
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 11498
Homologene: 86950
Mipep
Name: mitochondrial intermediate peptidase
Synonyms: 5730405E07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 70478
VEGA: 14
HGNC: HGNC:7104
Homologene: 4337
Socs1
Name: suppressor of cytokine signaling 1
Synonyms: SSI-1, JAK2-binding protein, STAT-induced STAT inhibitor 1, JAK-binding protein, JAB, Cish7, Cish1, SOCS-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12703
VEGA: 16
Homologene: 2776
Ptpn18
Name: protein tyrosine phosphatase, non-receptor type 18
Synonyms: HSCF, FLP1, Ptpk1, PTP-K1, PTP-HSCF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19253
HGNC: HGNC:9649
Homologene: 74971
Ubqln3
Name: ubiquilin 3
Synonyms: 4933400K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244178
Homologene: 9700
Hao1
Name: hydroxyacid oxidase 1, liver
Synonyms: GOX, Hao-1, Gox1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 15112
HGNC: HGNC:4809
Homologene: 6578
Rpl12-ps1
Name: ribosomal protein L12, pseudogene 1
Synonyms: Gm4957
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241053
Speer3
Name: spermatogenesis associated glutamate (E)-rich protein 3
Synonyms: SPEER-3, 4933405P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71026
Homologene: 69402
Sult2a7
Name: sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 7
Synonyms: Gm7231
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 638251
Mir6907
Name: microRNA 6907
Synonyms: mmu-mir-6907
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 102465547
VEGA: 10
Gm15032
Name: predicted gene 15032
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 100039086
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,462,742 bp
  • A to C, chromosome 1 at 36,958,099 bp
  • A to G, chromosome 1 at 155,290,161 bp
  • A to C, chromosome 2 at 20,530,206 bp
  • T to C, chromosome 2 at 24,396,335 bp
  • C to T, chromosome 2 at 40,600,380 bp
  • G to A, chromosome 2 at 79,273,112 bp
  • T to A, chromosome 2 at 134,523,044 bp
  • T to C, chromosome 3 at 105,032,747 bp
  • A to G, chromosome 3 at 154,234,073 bp
  • C to T, chromosome 4 at 32,715,883 bp
  • A to C, chromosome 4 at 133,695,699 bp
  • G to A, chromosome 4 at 145,062,602 bp
  • T to C, chromosome 4 at 156,217,586 bp
  • T to G, chromosome 5 at 13,796,354 bp
  • A to T, chromosome 5 at 38,417,230 bp
  • C to T, chromosome 5 at 74,620,192 bp
  • G to A, chromosome 6 at 4,518,822 bp
  • T to C, chromosome 6 at 48,596,526 bp
  • A to G, chromosome 6 at 120,350,258 bp
  • A to T, chromosome 6 at 139,720,006 bp
  • T to A, chromosome 7 at 14,470,161 bp
  • G to A, chromosome 7 at 104,141,444 bp
  • G to A, chromosome 7 at 141,638,400 bp
  • A to G, chromosome 8 at 108,793,779 bp
  • C to T, chromosome 9 at 20,348,723 bp
  • A to G, chromosome 9 at 83,426,648 bp
  • T to C, chromosome 10 at 50,842,243 bp
  • G to T, chromosome 10 at 78,412,822 bp
  • T to C, chromosome 10 at 87,576,215 bp
  • T to A, chromosome 11 at 54,926,790 bp
  • G to A, chromosome 11 at 70,681,018 bp
  • A to G, chromosome 11 at 109,966,755 bp
  • A to T, chromosome 12 at 81,421,747 bp
  • G to A, chromosome 12 at 99,955,065 bp
  • T to C, chromosome 12 at 113,490,352 bp
  • A to T, chromosome 14 at 20,515,501 bp
  • T to C, chromosome 14 at 30,096,971 bp
  • G to A, chromosome 14 at 56,156,753 bp
  • T to C, chromosome 14 at 60,827,321 bp
  • A to G, chromosome 14 at 65,028,932 bp
  • C to T, chromosome 16 at 10,784,354 bp
  • G to A, chromosome 16 at 37,048,563 bp
  • T to C, chromosome 16 at 84,827,974 bp
  • G to A, chromosome 17 at 43,085,388 bp
  • A to G, chromosome 17 at 75,500,673 bp
  • T to C, chromosome 18 at 22,067,392 bp
  • A to T, chromosome X at 142,622,626 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4509 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041758-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.