Strain Name:
C57BL/6J-MtgxR4521Btlr/Mmmh
Stock Number:
041764-MU
Citation ID:
RRID:MMRRC_041764-MU
Other Names:
R4521 (G1), C57BL/6J-MtgxR4521Btlr
Major Collection:

Gene Information

Ccdc88c
Name: coiled-coil domain containing 88C
Synonyms: 0610010D24Rik, Daple
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 68339
VEGA: 12
Homologene: 18903
Add1
Name: adducin 1 (alpha)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11518
HGNC: HGNC:243
Homologene: 22758
Stk17b
Name: serine/threonine kinase 17b (apoptosis-inducing)
Synonyms: Drak2, 3110009A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98267
Homologene: 31231
Nup93
Name: nucleoporin 93
Synonyms: 2410008G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 71805
Homologene: 40971
Orc4
Name: origin recognition complex, subunit 4
Synonyms: Orc4l, Orc4P, mMmORC4, Orc4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 26428
HGNC: HGNC:8490
Homologene: 8059
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: Helic1, B630009I04Rik, ASC1p200
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 77987
VEGA: 10
Homologene: 4973
Lrp6
Name: low density lipoprotein receptor-related protein 6
Synonyms: Cd, skam26Jus, ska26, skax26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16974
HGNC: HGNC:6698
Homologene: 1747
Topbp1
Name: topoisomerase (DNA) II binding protein 1
Synonyms: 1110031N14Rik, 2810429C13Rik, D430026L04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235559
Homologene: 38262
Bora
Name: bora, aurora kinase A activator
Synonyms: 6720463M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 77744
VEGA: 14
Homologene: 11728
Aco1
Name: aconitase 1
Synonyms: Aco-1, Irp1, Irebp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11428
HGNC: HGNC:117
Homologene: 1657
Akt2
Name: thymoma viral proto-oncogene 2
Synonyms: PKB, PKBbeta, 2410016A19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11652
HGNC: HGNC:392
Homologene: 48773
Glmp
Name: glycosylated lysosomal membrane protein
Synonyms: 0610031J06Rik, NCU-G1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 56700
Homologene: 10562
Cadm3
Name: cell adhesion molecule 3
Synonyms: SynCAM3, Necl1, Igsf4b, BIgR, Necl-1, Tsll1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 94332
Homologene: 10919
Car14
Name: carbonic anhydrase 14
Synonyms: CA XIV
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 23831
HGNC: HGNC:1372
Homologene: 69105
Grhl3
Name: grainyhead like transcription factor 3
Synonyms: Get1, ct, Som
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230824
Homologene: 18864
Nop2
Name: NOP2 nucleolar protein
Synonyms: 120kDa, Nol1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 110109
HGNC: HGNC:7867
Homologene: 135865
Dcaf6
Name: DDB1 and CUL4 associated factor 6
Synonyms: 1200006M05Rik, Iqwd1, PC326
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74106
Homologene: 10199
Ppwd1
Name: peptidylprolyl isomerase domain and WD repeat containing 1
Synonyms: 4632422M10Rik, A330090G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238831
VEGA: 13
Homologene: 9099
Rpl10l
Name: ribosomal protein L10-like
Synonyms: EG238217
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238217
Homologene: 68830
Ghr
Name: growth hormone receptor
Synonyms: GHR/BP, GHBP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 14600
HGNC: HGNC:4263
Homologene: 134
Ttn
Name: titin
Synonyms: 2310036G12Rik, 1100001C23Rik, mdm, connectin, D330041I19Rik, 2310057K23Rik, 2310074I15Rik, shru, L56, D830007G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
Fat3
Name: FAT atypical cadherin 3
Synonyms: LOC234973, 9430076A06Rik, D430038H04Rik, LOC382129
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270120
VEGA: 9
Homologene: 82252
Plpp2
Name: phospholipid phosphatase 2
Synonyms: Ppap2c, Lpp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 50784
HGNC: HGNC:9230
Homologene: 2752
Helz2
Name: helicase with zinc finger 2, transcriptional coactivator
Synonyms: BC006779
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 229003
Homologene: 14118
Dlgap2
Name: DLG associated protein 2
Synonyms: 6430596N04Rik, SAP90/PSD-95-associated protein 2, PSD-95/SAP90-binding protein 2, Sapap2, DAP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244310
HGNC: HGNC:2906
Homologene: 3484
Plce1
Name: phospholipase C, epsilon 1
Synonyms: PLCepsilon, 4933403A21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 74055
Homologene: 9478
Megf8
Name: multiple EGF-like-domains 8
Synonyms: b2b1702Clo, Egfl4, m687Ddg, b2b288Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269878
HGNC: HGNC:3233
Homologene: 15988
Map3k13
Name: mitogen-activated protein kinase kinase kinase 13
Synonyms: C130026N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 71751
VEGA: 16
HGNC: HGNC:6852
Homologene: 37958
Fbxo41
Name: F-box protein 41
Synonyms: D6Ertd538e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330369
Homologene: 19837
Tsen54
Name: tRNA splicing endonuclease subunit 54
Synonyms: 0610034P02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76265
Homologene: 35476
Cdc20b
Name: cell division cycle 20B
Synonyms: EG622422, EG238896
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238896
Homologene: 65056
Mrgprx1
Name: MAS-related GPR, member X1
Synonyms: Mrgprc11, MrgC11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 404242
Homologene: 86184
Lcp1
Name: lymphocyte cytosolic protein 1
Synonyms: D14Ertd310e, Pls2, L-plastin, L-fimbrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 18826
HGNC: HGNC:6528
Homologene: 80174
Lrfn2
Name: leucine rich repeat and fibronectin type III domain containing 2
Synonyms: SALM1, 5730420O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 70530
Homologene: 19182
Rbm20
Name: RNA binding motif protein 20
Synonyms: 1110018J23Rik, 2010003H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 73713
Homologene: 28386
Skida1
Name: SKI/DACH domain containing 1
Synonyms: 5730507N06Rik, 2810030E01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 72668
Homologene: 66327
Mrgpra9
Name: MAS-related GPR, member A9
Synonyms: EG668725, MrgA9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 668725
Homologene: 79615
Olfr623
Name: olfactory receptor 623
Synonyms: GA_x6K02T2PBJ9-6384836-6383883, MOR1-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259126
Homologene: 133591
Tmem38a
Name: transmembrane protein 38A
Synonyms: TRIC-A, 1110001E17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 74166
Homologene: 11449
Olfr810
Name: olfactory receptor 810
Synonyms: GA_x6K02T2PULF-11470271-11469333, MOR113-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258543
Homologene: 46347
Crb2
Name: crumbs family member 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241324
Homologene: 45474
Fpr2
Name: formyl peptide receptor 2
Synonyms: E330010I07Rik, Fpr-rs2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14289
HGNC: HGNC:3827
Homologene: 74395
Tapbpl
Name: TAP binding protein-like
Synonyms: TAPBPL-R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 213233
Homologene: 9958
4930455D15Rik
Name: RIKEN cDNA 4930455D15 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 75795
VEGA: 18
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 53,764,038 bp
  • A to T, chromosome 1 at 165,390,490 bp
  • A to T, chromosome 1 at 173,345,063 bp
  • T to C, chromosome 2 at 18,045,872 bp
  • T to C, chromosome 2 at 37,795,337 bp
  • G to A, chromosome 2 at 48,937,489 bp
  • G to A, chromosome 2 at 76,725,216 bp
  • T to C, chromosome 2 at 181,228,833 bp
  • C to A, chromosome 3 at 88,328,039 bp
  • A to G, chromosome 3 at 95,904,378 bp
  • C to T, chromosome 4 at 40,190,788 bp
  • T to C, chromosome 4 at 135,546,250 bp
  • C to T, chromosome 5 at 34,630,498 bp
  • A to G, chromosome 6 at 85,484,042 bp
  • G to T, chromosome 6 at 125,133,552 bp
  • A to G, chromosome 6 at 125,228,122 bp
  • A to G, chromosome 6 at 134,485,862 bp
  • T to A, chromosome 7 at 25,342,701 bp
  • T to C, chromosome 7 at 27,636,054 bp
  • A to T, chromosome 7 at 47,235,190 bp
  • T to C, chromosome 7 at 48,021,699 bp
  • C to T, chromosome 7 at 103,660,332 bp
  • G to A, chromosome 8 at 14,727,871 bp
  • C to T, chromosome 8 at 72,572,161 bp
  • T to C, chromosome 8 at 94,314,636 bp
  • T to C, chromosome 9 at 15,922,942 bp
  • C to T, chromosome 9 at 79,633,357 bp
  • T to C, chromosome 9 at 103,334,202 bp
  • A to G, chromosome 10 at 50,660,670 bp
  • A to G, chromosome 10 at 79,530,625 bp
  • T to A, chromosome 10 at 129,791,181 bp
  • G to A, chromosome 11 at 115,817,106 bp
  • T to C, chromosome 12 at 66,283,738 bp
  • G to T, chromosome 12 at 100,913,332 bp
  • A to G, chromosome 13 at 104,209,659 bp
  • A to G, chromosome 13 at 113,081,191 bp
  • A to G, chromosome 14 at 75,215,168 bp
  • G to T, chromosome 14 at 99,068,548 bp
  • A to G, chromosome 15 at 3,325,958 bp
  • T to C, chromosome 16 at 21,905,775 bp
  • A to C, chromosome 17 at 17,893,247 bp
  • T to A, chromosome 17 at 49,069,894 bp
  • G to A, chromosome 18 at 32,841,148 bp
  • T to C, chromosome 19 at 38,524,319 bp
  • A to G, chromosome 19 at 53,817,202 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4521 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041764-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.