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Strain Name:
C57BL/6J-MtgxR4544Btlr/Mmmh
Stock Number:
041779-MU
Citation ID:
RRID:MMRRC_041779-MU
Other Names:
R4544 (G1), C57BL/6J-MtgxR4544Btlr
Major Collection:

Strain Information

Pik3cb
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Synonyms: 1110001J02Rik, p110beta
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74769
HGNC: HGNC:8976
Homologene: 21250
Fancd2
Name: Fanconi anemia, complementation group D2
Synonyms: 2410150O07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 211651
HGNC: HGNC:3585
Homologene: 13212
Atr
Name: ataxia telangiectasia and Rad3 related
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245000
HGNC: HGNC:882
Homologene: 96916
Sorbs1
Name: sorbin and SH3 domain containing 1
Synonyms: c-Cbl-associated protein, CAP, Sh3d5, 9530001P15Rik, 2310065E01Rik, Ponsin
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20411
VEGA: 19
Homologene: 83252
Mfn2
Name: mitofusin 2
Synonyms: hypertension related protein 1, D630023P19Rik, Fzo
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 170731
Homologene: 8915
Ccnyl1
Name: cyclin Y-like 1
Synonyms: 9630037P07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227210
Homologene: 65050
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 64,723,576 bp
  • A to G, chromosome 1 at 87,184,642 bp
  • A to T, chromosome 1 at 121,312,192 bp
  • T to C, chromosome 1 at 173,582,127 bp
  • A to T, chromosome 1 at 173,875,664 bp
  • T to C, chromosome 1 at 174,026,573 bp
  • C to T, chromosome 2 at 69,712,311 bp
  • G to A, chromosome 2 at 69,956,093 bp
  • T to C, chromosome 2 at 76,584,492 bp
  • T to C, chromosome 2 at 76,822,588 bp
  • C to T, chromosome 2 at 103,976,037 bp
  • T to C, chromosome 3 at 86,999,214 bp
  • C to A, chromosome 3 at 106,210,606 bp
  • C to T, chromosome 4 at 3,973,244 bp
  • G to A, chromosome 4 at 101,768,228 bp
  • A to G, chromosome 4 at 147,887,452 bp
  • T to C, chromosome 4 at 155,070,495 bp
  • C to T, chromosome 5 at 65,164,690 bp
  • T to G, chromosome 5 at 108,847,685 bp
  • G to A, chromosome 5 at 120,531,153 bp
  • A to G, chromosome 5 at 127,604,536 bp
  • C to G, chromosome 5 at 137,383,834 bp
  • T to C, chromosome 6 at 30,740,680 bp
  • T to C, chromosome 6 at 39,175,472 bp
  • A to G, chromosome 6 at 40,493,808 bp
  • C to T, chromosome 6 at 42,927,414 bp
  • A to G, chromosome 6 at 113,572,642 bp
  • A to G, chromosome 6 at 122,626,767 bp
  • A to G, chromosome 6 at 124,531,540 bp
  • T to C, chromosome 7 at 18,478,539 bp
  • C to T, chromosome 7 at 30,971,976 bp
  • A to T, chromosome 7 at 81,328,099 bp
  • A to T, chromosome 7 at 86,921,191 bp
  • A to G, chromosome 7 at 93,052,980 bp
  • A to T, chromosome 8 at 16,710,636 bp
  • C to T, chromosome 8 at 61,016,304 bp
  • T to C, chromosome 8 at 71,327,941 bp
  • A to G, chromosome 8 at 77,674,911 bp
  • C to T, chromosome 9 at 50,805,354 bp
  • T to A, chromosome 9 at 56,888,629 bp
  • A to T, chromosome 9 at 95,862,895 bp
  • T to A, chromosome 9 at 99,039,759 bp
  • T to C, chromosome 10 at 127,790,837 bp
  • A to C, chromosome 10 at 129,238,158 bp
  • G to A, chromosome 11 at 99,478,276 bp
  • A to G, chromosome 12 at 26,478,017 bp
  • T to A, chromosome 12 at 104,959,469 bp
  • A to C, chromosome 13 at 4,507,844 bp
  • G to A, chromosome 13 at 93,091,918 bp
  • C to G, chromosome 14 at 54,735,790 bp
  • A to G, chromosome 15 at 102,534,327 bp
  • T to A, chromosome 17 at 7,773,544 bp
  • A to T, chromosome 17 at 78,492,959 bp
  • T to C, chromosome 18 at 7,000,651 bp
  • G to A, chromosome 19 at 40,311,850 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4544 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041779-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.