Strain Name:
C57BL/6J-MtgxR4568Btlr/Mmmh
Stock Number:
041792-MU
Citation ID:
RRID:MMRRC_041792-MU
Other Names:
R4568 (G1), C57BL/6J-MtgxR4568Btlr
Major Collection:

Gene Information

Pik3cb
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Synonyms: p110beta, 1110001J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74769
HGNC: HGNC:8976
Homologene: 21250
Lgals8
Name: lectin, galactose binding, soluble 8
Synonyms: 1200015E08Rik, D13Ertd524e, Lgals-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 56048
HGNC: HGNC:6569
Homologene: 31386
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: Dlgh1, B130052P05Rik, SAP97
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869
Col6a1
Name: collagen, type VI, alpha 1
Synonyms: Col6a-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12833
HGNC: HGNC:2211
Homologene: 1391
Gpr139
Name: G protein-coupled receptor 139
Synonyms: GPRg1, LOC209776
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 209776
Homologene: 45860
Tpr
Name: translocated promoter region, nuclear basket protein
Synonyms: 2610029M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 108989
Homologene: 37753
Tasor
Name: transcription activation suppressor
Synonyms: D14Abb1e, Fam208a, 4933409E02Rik, MommeD6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218850
VEGA: 14
Homologene: 9062
Stag1
Name: stromal antigen 1
Synonyms: Scc3, SA-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20842
Homologene: 21191
Plxna4
Name: plexin A4
Synonyms: Plxa4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243743
HGNC: HGNC:9102
Homologene: 77587
Dnah8
Name: dynein, axonemal, heavy chain 8
Synonyms: Hst6.7b, Dnahc8, P1-Loop
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13417
VEGA: 17
HGNC: HGNC:2952
Homologene: 1049
Dgcr8
Name: DGCR8, microprocessor complex subunit
Synonyms: Vo59c07, N41, D16H22S788E, D16H22S1742E, D16Wis2, DiGeorge syndrome critical region gene 8, Gy1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 94223
HGNC: HGNC:2847
Homologene: 11223
Robo4
Name: roundabout guidance receptor 4
Synonyms: Magic roundabout, 1200012D01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74144
Homologene: 10397
Ptpn4
Name: protein tyrosine phosphatase, non-receptor type 4
Synonyms: PTPMEG, protein tyrosine phosphatase, non-receptor type 4 (megakaryocyte), TEP/mPTPMEG, testis-enriched phosphatase, TEP, hPTP-MEG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19258
HGNC: HGNC:9656
Homologene: 2120
Bik
Name: BCL2-interacting killer
Synonyms: Nbk, Biklk, Blk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 12124
VEGA: 15
HGNC: HGNC:1051
Homologene: 924
Map4k1
Name: mitogen-activated protein kinase kinase kinase kinase 1
Synonyms: Hpk1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26411
HGNC: HGNC:6863
Homologene: 5199
Csn1s1
Name: casein alpha s1
Synonyms: Csna
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12990
HGNC: HGNC:2445
Mfsd6
Name: major facilitator superfamily domain containing 6
Synonyms: 2210010L05Rik, MMR2, 9630025I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98682
Homologene: 9784
Vmn2r63
Name: vomeronasal 2, receptor 63
Synonyms: EG435975
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 435975
Homologene: 104832
Tbc1d9
Name: TBC1 domain family, member 9
Synonyms: C76116, 4933431N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 71310
Homologene: 57079
Krba1
Name: KRAB-A domain containing 1
Synonyms: A930040G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 77827
Homologene: 15880
Spef2
Name: sperm flagellar 2
Synonyms: C230086A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 320277
Homologene: 23371
Pla2g4a
Name: phospholipase A2, group IVA (cytosolic, calcium-dependent)
Synonyms: cPLA2, cytosolic PLA2, cytosolic phospholipase A2, Type IV PLA2, cPLA2alpha, Pla2g4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18783
HGNC: HGNC:9035
Homologene: 32059
Phldb2
Name: pleckstrin homology like domain, family B, member 2
Synonyms: LL5b, C820004H04Rik, LL5beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 208177
Homologene: 17100
Olfr412
Name: olfactory receptor 412
Synonyms: MOR127-5P, GA_x6K02T2P1NL-4500587-4501525
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258153
HGNC: HGNC:8183
Homologene: 37634
Pga5
Name: pepsinogen 5, group I
Synonyms: pepsinogen A5, Pepf, 1110035E17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 58803
VEGA: 19
Homologene: 105932
Ripply3
Name: ripply transcriptional repressor 3
Synonyms: Dscr6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 170765
HGNC: HGNC:3047
Homologene: 10350
Vmn2r65
Name: vomeronasal 2, receptor 65
Synonyms: ENSMUSG00000070600
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100009609
Ctnna3
Name: catenin (cadherin associated protein), alpha 3
Synonyms: alphaT-catenin, Vr22, Catna3, 4930429L08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216033
HGNC: HGNC:2511
Homologene: 56583
Slc5a9
Name: solute carrier family 5 (sodium/glucose cotransporter), member 9
Synonyms: SGLT4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230612
Homologene: 17081
Acsm2
Name: acyl-CoA synthetase medium-chain family member 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233799
Homologene: 70404
Omt2b
Name: oocyte maturation, beta
Synonyms: OM2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382088
Homologene: 136010
Trpv6
Name: transient receptor potential cation channel, subfamily V, member 6
Synonyms: Cac, CaT1, Ecac2, CAT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 64177
Homologene: 56812
Rasl2-9
Name: RAS-like, family 2, locus 9
Synonyms: Rasl2-9-ps, Ran/M2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19428
Homologene: 135303
Olfr1411
Name: olfactory receptor 1411
Synonyms: MOR208-3, GA_x6K02T2R7CC-81157497-81156526
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 258483
Homologene: 115530
Psmg3
Name: proteasome (prosome, macropain) assembly chaperone 3
Synonyms: 1810042K04Rik, 4930403H09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 66506
Homologene: 11980
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 52,663,289 bp
  • A to T, chromosome 1 at 92,597,391 bp
  • T to C, chromosome 1 at 119,680,059 bp
  • T to C, chromosome 1 at 149,842,226 bp
  • T to C, chromosome 1 at 150,392,959 bp
  • C to A, chromosome 4 at 111,891,744 bp
  • G to A, chromosome 5 at 87,680,904 bp
  • A to G, chromosome 5 at 139,826,249 bp
  • T to A, chromosome 6 at 32,152,938 bp
  • G to T, chromosome 6 at 41,626,569 bp
  • A to G, chromosome 6 at 48,409,723 bp
  • AGG to A, chromosome 7 at 5,125,375 bp
  • G to A, chromosome 7 at 28,986,654 bp
  • T to C, chromosome 7 at 42,933,826 bp
  • C to T, chromosome 7 at 84,947,469 bp
  • C to T, chromosome 7 at 119,144,805 bp
  • T to A, chromosome 7 at 119,563,517 bp
  • A to G, chromosome 8 at 83,271,177 bp
  • T to C, chromosome 9 at 37,404,822 bp
  • T to A, chromosome 9 at 78,328,247 bp
  • T to C, chromosome 9 at 99,090,302 bp
  • T to C, chromosome 9 at 100,848,669 bp
  • C to T, chromosome 10 at 63,852,809 bp
  • A to T, chromosome 10 at 76,719,197 bp
  • A to G, chromosome 11 at 74,365,209 bp
  • T to G, chromosome 13 at 12,453,373 bp
  • A to T, chromosome 14 at 27,476,701 bp
  • T to C, chromosome 15 at 9,647,217 bp
  • T to C, chromosome 15 at 83,541,444 bp
  • G to A, chromosome 16 at 18,280,394 bp
  • T to C, chromosome 16 at 31,853,871 bp
  • T to A, chromosome 16 at 45,777,718 bp
  • T to A, chromosome 16 at 94,335,829 bp
  • G to A, chromosome 17 at 30,748,568 bp
  • A to G, chromosome 18 at 3,299,175 bp
  • A to G, chromosome 19 at 10,671,852 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4568 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041792-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.