Strain Name:
C57BL/6J-MtgxR4571Btlr/Mmmh
Stock Number:
041795-MU
Citation ID:
RRID:MMRRC_041795-MU
Other Names:
R4571 (G1), C57BL/6J-MtgxR4571Btlr
Major Collection:

Gene Information

Edn3
Name: endothelin 3
Synonyms: 114CH19, 114-CH19, tmgc48
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13616
HGNC: HGNC:3178
Homologene: 88
Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: Gena 52, GENA 60, GENA 47, Cyn, A730019I05Rik, WBE1, Whi, Todo, Obt, Mt, Lda, Flo, Edy, Dz, Cycn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 320790
Homologene: 19067
Pik3cb
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Synonyms: 1110001J02Rik, p110beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74769
HGNC: HGNC:8976
Homologene: 21250
Lars
Name: leucyl-tRNA synthetase
Synonyms: 3110009L02Rik, 2310045K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 107045
VEGA: 18
HGNC: HGNC:6512
Homologene: 7083
Mfn1
Name: mitofusin 1
Synonyms: 6330416C07Rik, 2310002F04Rik, D3Ertd265e, HR2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67414
Homologene: 11481
Ebag9
Name: estrogen receptor-binding fragment-associated gene 9
Synonyms: Rcas1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 55960
VEGA: 15
HGNC: HGNC:3123
Homologene: 3107
Clasp2
Name: CLIP associating protein 2
Synonyms: 1500004F14Rik, CLASP2gamma, CLASP2beta, CLASP2alpha, CLASP2, 8030404L10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 76499
VEGA: 9
Homologene: 24944
Pan2
Name: PAN2 poly(A) specific ribonuclease subunit
Synonyms: 1200014O24Rik, Usp52
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 103135
VEGA: 10
Homologene: 5918
Eif3e
Name: eukaryotic translation initiation factor 3, subunit E
Synonyms: eIF3-p48, eIF3-p46, Int6, 48kDa, Eif3s6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16341
VEGA: 15
HGNC: HGNC:3277
Homologene: 1205
Smg1
Name: SMG1 nonsense mediated mRNA decay associated PI3K related kinase
Synonyms: C130002K18Rik, 5430435M13Rik, 2610207I05Rik, SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233789
Homologene: 56697
Brca1
Name: breast cancer 1, early onset
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12189
HGNC: HGNC:1100
Homologene: 5276
Zfp30
Name: zinc finger protein 30
Synonyms: Zfp-30, 2610306P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22693
Homologene: 84799
Vps8
Name: VPS8 CORVET complex subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 209018
Homologene: 44592
Polg
Name: polymerase (DNA directed), gamma
Synonyms: Pol gamma, polymerase gamma, mitochondrial DNA polymerase gamma, mitochondrial DNA polymerase-gamma, Polga
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18975
HGNC: HGNC:9179
Homologene: 2016
Gabbr1
Name: gamma-aminobutyric acid (GABA) B receptor, 1
Synonyms: GABAbR1, GABAB1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 54393
HGNC: HGNC:4070
Homologene: 1132
Lmo7
Name: LIM domain only 7
Synonyms: LOC380928, FBXO20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 380928
HGNC: HGNC:6646
Homologene: 83924
Clec4g
Name: C-type lectin domain family 4, member g
Synonyms: 4930572L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 75863
Homologene: 18879
Nop2
Name: NOP2 nucleolar protein
Synonyms: 120kDa, Nol1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 110109
HGNC: HGNC:7867
Homologene: 135865
Btf3
Name: basic transcription factor 3
Synonyms: 1700054E11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218490
HGNC: HGNC:1125
Homologene: 37453
Gin1
Name: gypsy retrotransposon integrase 1
Synonyms: 4930429M06, 4930429M06Rik, Zh2c2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 252876
Homologene: 69238
Ddx11
Name: DEAD/H box helicase 11
Synonyms: CHLR1, KRG2, CHL1, 4732462I11Rik, essa15a, DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 320209
VEGA: 17
HGNC: HGNC:2736
Homologene: 68973
Zcchc2
Name: zinc finger, CCHC domain containing 2
Synonyms: 9930114B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227449
Homologene: 9808
Plxna2
Name: plexin A2
Synonyms: OCT, Plxn2, 2810428A13Rik, PlexA2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18845
HGNC: HGNC:9100
Homologene: 56427
Cacna1c
Name: calcium channel, voltage-dependent, L type, alpha 1C subunit
Synonyms: (alpha)1 subunit, Cchl1a1, Cav1.2, L-type Cav1.2, D930026N18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12288
HGNC: HGNC:1390
Homologene: 55484
Pkhd1
Name: polycystic kidney and hepatic disease 1
Synonyms: tigmin, FPC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241035
HGNC: HGNC:9016
Homologene: 16336
Tapbp
Name: TAP binding protein
Synonyms: tapasin, TPN, D17Wsu91e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 21356
Homologene: 2401
Kdm5d
Name: lysine (K)-specific demethylase 5D
Synonyms: Smcy, Jarid1d, HY
Type: Gene
Species: Mus musculus (mouse)
Chromosome: Y
NCBI: 20592
Homologene: 55838
Insrr
Name: insulin receptor-related receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 23920
HGNC: HGNC:6093
Homologene: 56539
Slc6a18
Name: solute carrier family 6 (neurotransmitter transporter), member 18
Synonyms: XT2, D630001K16Rik, Xtrp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 22598
VEGA: 13
Homologene: 40785
Col9a3
Name: collagen, type IX, alpha 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12841
HGNC: HGNC:2219
Homologene: 20438
Hrg
Name: histidine-rich glycoprotein
Synonyms: D16JH2, D18020
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 94175
HGNC: HGNC:5181
Homologene: 137650
Abca8a
Name: ATP-binding cassette, sub-family A (ABC1), member 8a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217258
HGNC: HGNC:38
Homologene: 131160
Topaz1
Name: testis and ovary specific PAZ domain containing 1
Synonyms: Gm9524
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 671232
VEGA: 9
Homologene: 19835
Apbb1
Name: amyloid beta (A4) precursor protein-binding, family B, member 1
Synonyms: Fe65, Rir
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11785
HGNC: HGNC:581
Homologene: 898
Fam76a
Name: family with sequence similarity 76, member A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230789
Homologene: 27071
Csmd2
Name: CUB and Sushi multiple domains 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329942
Homologene: 89034
Rem2
Name: rad and gem related GTP binding protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 140743
Homologene: 11222
Olfr1115
Name: olfactory receptor 1115
Synonyms: GA_x6K02T2Q125-48736906-48737886, MOR273-4P, MOR273-4P, MOR264-20, Olfr1530-ps1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258294
Homologene: 27114
C2
Name: complement component 2 (within H-2S)
Synonyms: classical-complement pathway C3/C5 convertase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12263
HGNC: HGNC:1248
Homologene: 45
Galc
Name: galactosylceramidase
Synonyms: Gacy, 2310068B06Rik, galactocerebrosidase, A930008M05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 14420
VEGA: 12
HGNC: HGNC:4115
Homologene: 124
Zfp558
Name: zinc finger protein 558
Synonyms: 1700007A21Rik, 4932704I17Rik, Zfp558-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72230
Homologene: 44903
Ttbk2
Name: tau tubulin kinase 2
Synonyms: TTK, B930008N24Rik, 2610507N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 140810
Homologene: 62795
1700123L14Rik
Name: RIKEN cDNA 1700123L14 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 78482
Gimap3
Name: GTPase, IMAP family member 3
Synonyms: 2010110D23Rik, Ian4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 83408
Homologene: 41257
Kcnq3
Name: potassium voltage-gated channel, subfamily Q, member 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 110862
HGNC: HGNC:6297
Homologene: 20949
Gm7052
Name: predicted gene 7052
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 631010
Gpcpd1
Name: glycerophosphocholine phosphodiesterase 1
Synonyms: 2310032D16Rik, 2310004G06Rik, Prei4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74182
Homologene: 10478
Zfp62
Name: zinc finger protein 62
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 22720
Homologene: 40686
Zp3r
Name: zona pellucida 3 receptor
Synonyms: SP56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22789
Homologene: 7609
Acoxl
Name: acyl-Coenzyme A oxidase-like
Synonyms: 1200014P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74121
Homologene: 75083
Kcnd3
Name: potassium voltage-gated channel, Shal-related family, member 3
Synonyms: potassium channel Kv4.3M, potassium channel Kv4.3L, Kv4.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 56543
HGNC: HGNC:6239
Homologene: 21036
Apol7b
Name: apolipoprotein L 7b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 278679
Homologene: 40940
Map3k9
Name: mitogen-activated protein kinase kinase kinase 9
Synonyms: Mlk1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 338372
VEGA: 12
HGNC: HGNC:6861
Homologene: 76377
Ipp
Name: IAP promoted placental gene
Synonyms: D4Jhu8, Mipp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 16351
HGNC: HGNC:6108
Homologene: 4309
Hoxb4
Name: homeobox B4
Synonyms: Hox-2.6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15412
HGNC: HGNC:5115
Homologene: 32095
Olfr420
Name: olfactory receptor 420
Synonyms: GA_x6K02T2P20D-20995211-20994246, MOR105-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 258302
Homologene: 17185
Gm4868
Name: predicted gene 4868
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231736
Homologene: 138658
Trpv6
Name: transient receptor potential cation channel, subfamily V, member 6
Synonyms: CaT1, Cac, CAT, Ecac2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 64177
Homologene: 56812
Dnah7c
Name: dynein, axonemal, heavy chain 7C
Synonyms: Dnahc7c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100101919
Homologene: 41287
Olfr1029
Name: olfactory receptor 1029
Synonyms: GA_x6K02T2Q125-47454152-47455126, MOR198-1P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258154
Homologene: 73972
Vmn1r188
Name: vomeronasal 1 receptor 188
Synonyms: V1rh17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 252912
Homologene: 110880
Slco4a1
Name: solute carrier organic anion transporter family, member 4a1
Synonyms: OATP-E, Slc21a12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 108115
Homologene: 9482
Arl4d
Name: ADP-ribosylation factor-like 4D
Synonyms: Arl5, 1110036H21Rik, Arf4l, Arfl4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 80981
HGNC: HGNC:656
Homologene: 1255
Olfr90
Name: olfactory receptor 90
Synonyms: MOR256-21, GA_x6K02T2PSCP-1526227-1525295
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258469
HGNC: HGNC:8253
Homologene: 68546
Wnt9a
Name: wingless-type MMTV integration site family, member 9A
Synonyms: Wnt14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216795
Homologene: 20722
Pcmtd2
Name: protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 2
Synonyms: 5330414D10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 245867
Homologene: 10099
Rpl5-ps2
Name: ribisomal protein L5, pseudogene 2
Synonyms: Gm14217
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 668936
Homologene: 138654
Dusp23
Name: dual specificity phosphatase 23
Synonyms: 1300005N15Rik, Ldp-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 68440
Homologene: 32368
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 20,613,409 bp
  • A to C, chromosome 1 at 46,533,216 bp
  • T to C, chromosome 1 at 97,785,076 bp
  • A to G, chromosome 1 at 106,031,257 bp
  • A to G, chromosome 1 at 130,578,020 bp
  • A to C, chromosome 1 at 172,632,614 bp
  • A to T, chromosome 1 at 174,158,928 bp
  • T to C, chromosome 1 at 194,810,988 bp
  • A to T, chromosome 2 at 85,975,831 bp
  • C to A, chromosome 2 at 87,252,458 bp
  • C to T, chromosome 2 at 120,822,608 bp
  • G to T, chromosome 2 at 127,877,807 bp
  • T to A, chromosome 2 at 132,550,350 bp
  • G to T, chromosome 2 at 154,704,236 bp
  • G to A, chromosome 2 at 174,781,904 bp
  • A to T, chromosome 2 at 180,464,378 bp
  • T to C, chromosome 2 at 180,616,366 bp
  • A to G, chromosome 2 at 181,842,424 bp
  • T to C, chromosome 3 at 32,531,977 bp
  • A to G, chromosome 3 at 87,800,887 bp
  • C to T, chromosome 3 at 105,658,766 bp
  • C to A, chromosome 4 at 8,866,217 bp
  • A to G, chromosome 4 at 116,530,458 bp
  • T to A, chromosome 4 at 128,480,095 bp
  • T to C, chromosome 4 at 132,920,897 bp
  • A to G, chromosome 5 at 125,848,718 bp
  • C to T, chromosome 6 at 41,621,744 bp
  • T to C, chromosome 6 at 48,765,720 bp
  • T to A, chromosome 6 at 96,164,881 bp
  • T to C, chromosome 6 at 118,630,380 bp
  • T to G, chromosome 6 at 125,140,881 bp
  • A to G, chromosome 7 at 29,793,202 bp
  • T to C, chromosome 7 at 79,460,379 bp
  • T to A, chromosome 7 at 105,573,762 bp
  • A to T, chromosome 7 at 118,139,465 bp
  • A to T, chromosome 8 at 3,718,766 bp
  • A to T, chromosome 9 at 18,456,503 bp
  • T to A, chromosome 9 at 99,090,257 bp
  • G to T, chromosome 9 at 113,847,721 bp
  • A to G, chromosome 9 at 122,747,436 bp
  • A to G, chromosome 10 at 128,308,643 bp
  • A to C, chromosome 11 at 49,215,741 bp
  • G to T, chromosome 11 at 49,215,742 bp
  • T to C, chromosome 11 at 59,331,337 bp
  • T to C, chromosome 11 at 96,319,166 bp
  • C to A, chromosome 11 at 101,517,366 bp
  • T to A, chromosome 11 at 101,667,143 bp
  • A to T, chromosome 11 at 110,030,058 bp
  • C to A, chromosome 12 at 81,734,091 bp
  • T to C, chromosome 12 at 98,222,617 bp
  • A to G, chromosome 13 at 22,088,518 bp
  • A to T, chromosome 13 at 73,666,370 bp
  • A to G, chromosome 13 at 98,313,284 bp
  • T to C, chromosome 14 at 54,477,659 bp
  • G to T, chromosome 14 at 101,887,594 bp
  • G to A, chromosome 15 at 43,266,162 bp
  • A to T, chromosome 15 at 44,636,762 bp
  • A to G, chromosome 15 at 66,030,612 bp
  • T to C, chromosome 15 at 77,423,534 bp
  • T to A, chromosome 16 at 21,435,775 bp
  • C to T, chromosome 16 at 22,961,222 bp
  • T to A, chromosome 17 at 22,040,424 bp
  • C to A, chromosome 17 at 33,926,453 bp
  • T to C, chromosome 17 at 34,863,659 bp
  • G to T, chromosome 17 at 37,054,236 bp
  • T to C, chromosome 17 at 37,085,579 bp
  • T to C, chromosome 17 at 66,130,773 bp
  • T to C, chromosome 18 at 42,228,230 bp
  • T to C, chromosome Y at 927,110 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4571 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041795-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.