Strain Name:
C57BL/6J-MtgxR4583Btlr/Mmmh
Stock Number:
041804-MU
Citation ID:
RRID:MMRRC_041804-MU
Other Names:
R4583 (G1), C57BL/6J-MtgxR4583Btlr
Major Collection:

Gene Information

Gene Symbol: Dnm2 [MGI:109547] (Mus musculus (mouse))
Name: dynamin 2
Synonyms: b2b2159Clo, Dyn2
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Sema6d [MGI:2387661] (Mus musculus (mouse))
Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Synonyms: Sema6D-1, Sema6D-5, Sema6D-6, 1110067B02Rik, Sema6D-2, Sema6D-4
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Colgalt2 [MGI:2138232] (Mus musculus (mouse))
Name: collagen beta(1-O)galactosyltransferase 2
Synonyms: Glt25d2
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Pdgfc [MGI:1859631] (Mus musculus (mouse))
Name: platelet-derived growth factor, C polypeptide
Synonyms: PDGF-C, 1110064L01Rik
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Ncam2 [MGI:97282] (Mus musculus (mouse))
Name: neural cell adhesion molecule 2
Synonyms: Ocam, R4B12 antigen, Ncam-2, RNCAM
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Slc35a1 [MGI:1345622] (Mus musculus (mouse))
Name: solute carrier family 35 (CMP-sialic acid transporter), member 1
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Cr1l [MGI:88513] (Mus musculus (mouse))
Name: complement component (3b/4b) receptor 1-like
Synonyms: Crry, mCRY
Chromosome: 1
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 21,013,449 bp
  • T to G, chromosome 1 at 24,561,329 bp
  • T to C, chromosome 1 at 36,317,664 bp
  • C to T, chromosome 1 at 86,386,645 bp
  • C to T, chromosome 1 at 118,842,068 bp
  • C to T, chromosome 1 at 152,506,876 bp
  • T to A, chromosome 1 at 153,460,303 bp
  • A to G, chromosome 1 at 156,065,142 bp
  • A to G, chromosome 1 at 170,966,555 bp
  • T to C, chromosome 1 at 195,129,831 bp
  • C to A, chromosome 2 at 27,062,280 bp
  • A to G, chromosome 2 at 30,091,484 bp
  • C to T, chromosome 2 at 31,002,930 bp
  • A to G, chromosome 2 at 31,413,265 bp
  • A to T, chromosome 2 at 35,028,177 bp
  • A to C, chromosome 2 at 36,640,614 bp
  • A to G, chromosome 2 at 59,878,317 bp
  • A to G, chromosome 2 at 75,663,606 bp
  • A to G, chromosome 2 at 82,978,673 bp
  • T to C, chromosome 2 at 87,783,221 bp
  • G to T, chromosome 2 at 88,865,494 bp
  • T to A, chromosome 2 at 88,959,212 bp
  • A to T, chromosome 2 at 92,458,921 bp
  • G to T, chromosome 2 at 124,664,162 bp
  • C to T, chromosome 2 at 143,988,751 bp
  • G to A, chromosome 2 at 156,003,205 bp
  • T to C, chromosome 2 at 161,014,194 bp
  • A to T, chromosome 2 at 168,689,360 bp
  • A to T, chromosome 3 at 79,628,580 bp
  • A to T, chromosome 3 at 81,141,528 bp
  • G to T, chromosome 3 at 107,211,402 bp
  • C to T, chromosome 3 at 132,677,047 bp
  • A to G, chromosome 3 at 135,485,713 bp
  • A to G, chromosome 4 at 11,810,466 bp
  • T to A, chromosome 4 at 34,664,146 bp
  • A to G, chromosome 4 at 43,002,991 bp
  • C to A, chromosome 4 at 43,633,522 bp
  • A to G, chromosome 4 at 43,855,262 bp
  • T to C, chromosome 4 at 43,931,062 bp
  • C to T, chromosome 4 at 116,158,494 bp
  • T to A, chromosome 4 at 124,707,549 bp
  • T to C, chromosome 4 at 139,380,853 bp
  • A to G, chromosome 4 at 143,416,754 bp
  • T to G, chromosome 4 at 148,051,872 bp
  • T to A, chromosome 4 at 148,164,899 bp
  • T to C, chromosome 4 at 149,469,151 bp
  • A to T, chromosome 5 at 45,733,589 bp
  • T to A, chromosome 5 at 53,699,782 bp
  • C to T, chromosome 5 at 74,610,796 bp
  • T to A, chromosome 5 at 97,108,210 bp
  • G to T, chromosome 6 at 39,522,842 bp
  • A to T, chromosome 6 at 68,017,458 bp
  • T to C, chromosome 6 at 70,143,620 bp
  • T to A, chromosome 6 at 71,326,097 bp
  • T to C, chromosome 6 at 120,492,094 bp
  • T to G, chromosome 6 at 124,703,323 bp
  • C to T, chromosome 6 at 131,685,435 bp
  • T to G, chromosome 7 at 17,095,172 bp
  • G to T, chromosome 7 at 19,697,498 bp
  • T to C, chromosome 7 at 21,349,719 bp
  • C to T, chromosome 7 at 23,606,662 bp
  • T to A, chromosome 7 at 24,912,571 bp
  • A to G, chromosome 7 at 28,445,944 bp
  • G to T, chromosome 7 at 35,197,952 bp
  • C to A, chromosome 7 at 44,538,913 bp
  • C to T, chromosome 7 at 59,286,063 bp
  • A to G, chromosome 7 at 83,682,899 bp
  • T to A, chromosome 7 at 86,164,082 bp
  • A to G, chromosome 7 at 112,412,947 bp
  • A to G, chromosome 7 at 122,457,224 bp
  • AAAGAAGAAGAAGAAGAAG to AAAGAAGAAGAAGAAG, chromosome 7 at 123,001,952 bp
  • A to G, chromosome 8 at 13,409,322 bp
  • C to G, chromosome 8 at 20,900,681 bp
  • G to A, chromosome 8 at 22,218,420 bp
  • T to A, chromosome 8 at 25,710,676 bp
  • T to C, chromosome 8 at 80,613,514 bp
  • C to T, chromosome 8 at 110,595,225 bp
  • G to A, chromosome 8 at 122,865,028 bp
  • A to G, chromosome 9 at 21,504,446 bp
  • G to T, chromosome 9 at 42,977,662 bp
  • G to T, chromosome 9 at 79,815,809 bp
  • A to G, chromosome 9 at 124,029,440 bp
  • T to C, chromosome 10 at 43,997,620 bp
  • A to G, chromosome 10 at 111,301,134 bp
  • T to C, chromosome 10 at 129,842,475 bp
  • T to A, chromosome 11 at 67,096,453 bp
  • T to C, chromosome 11 at 73,887,803 bp
  • A to G, chromosome 11 at 75,671,862 bp
  • G to A, chromosome 11 at 76,212,638 bp
  • A to T, chromosome 11 at 83,397,779 bp
  • A to T, chromosome 11 at 83,648,338 bp
  • A to T, chromosome 11 at 106,407,205 bp
  • G to A, chromosome 11 at 107,646,069 bp
  • T to C, chromosome 12 at 54,922,540 bp
  • A to T, chromosome 13 at 30,947,518 bp
  • G to A, chromosome 13 at 55,421,130 bp
  • A to T, chromosome 13 at 92,468,109 bp
  • A to T, chromosome 14 at 47,381,687 bp
  • A to G, chromosome 15 at 79,715,951 bp
  • T to C, chromosome 15 at 81,684,906 bp
  • T to C, chromosome 15 at 101,946,620 bp
  • T to C, chromosome 16 at 5,104,536 bp
  • A to G, chromosome 16 at 32,877,884 bp
  • T to A, chromosome 16 at 34,235,267 bp
  • A to G, chromosome 16 at 36,761,380 bp
  • A to G, chromosome 16 at 81,517,557 bp
  • T to C, chromosome 17 at 7,739,249 bp
  • A to G, chromosome 17 at 20,570,453 bp
  • T to C, chromosome 17 at 21,129,730 bp
  • C to T, chromosome 17 at 23,819,619 bp
  • T to C, chromosome 17 at 26,196,502 bp
  • T to G, chromosome 17 at 28,982,156 bp
  • C to A, chromosome 17 at 29,367,078 bp
  • A to G, chromosome 17 at 45,589,956 bp
  • A to G, chromosome 17 at 46,161,145 bp
  • G to A, chromosome 17 at 74,258,461 bp
  • T to C, chromosome 18 at 13,844,330 bp
  • A to T, chromosome 18 at 37,475,575 bp
  • T to C, chromosome 18 at 60,246,080 bp
  • G to C, chromosome 19 at 3,265,324 bp
  • G to A, chromosome 19 at 7,204,436 bp
  • A to T, chromosome 19 at 10,968,189 bp
  • T to A, chromosome 19 at 13,190,698 bp
  • T to C, chromosome 19 at 47,837,216 bp
  • T to A, chromosome X at 155,125,629 bp
  • T to A, chromosome Y at 914,134 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4583 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041804-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.