Strain Name:
C57BL/6J-MtgxR4611Btlr/Mmmh
Stock Number:
041822-MU
Citation ID:
RRID:MMRRC_041822-MU
Other Names:
R4611 (G1), C57BL/6J-MtgxR4611Btlr
Major Collection:

Gene Information

Gene Symbol: Fn1 [MGI:95566] (Mus musculus (mouse))
Name: fibronectin 1
Synonyms: Fn-1, Fn
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Gpr37 [MGI:1313297] (Mus musculus (mouse))
Name: G protein-coupled receptor 37
Synonyms: Pael-R, parkin-associated endothelin B-like receptor
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Ptch2 [MGI:1095405] (Mus musculus (mouse))
Name: patched 2
Synonyms: ptc2
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Gria2 [MGI:95809] (Mus musculus (mouse))
Name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Synonyms: Glur-2, GluR2, GluR-B, GluA2, Glur2
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Atp1a1 [MGI:88105] (Mus musculus (mouse))
Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide
Synonyms: Atpa-1
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Zfp423 [MGI:1891217] (Mus musculus (mouse))
Name: zinc finger protein 423
Synonyms: ataxia1, Roaz, Ebfaz, Zfp104
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Rai14 [MGI:1922896] (Mus musculus (mouse))
Name: retinoic acid induced 14
Synonyms: Ankycorbin, 1700020L11Rik, 1700008J19Rik, Norpeg
Chromosome: 15
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 71,624,178 bp
  • T to A, chromosome 1 at 106,949,153 bp
  • T to A, chromosome 1 at 156,284,374 bp
  • T to A, chromosome 1 at 170,644,947 bp
  • T to A, chromosome 1 at 173,589,914 bp
  • G to A, chromosome 1 at 185,272,597 bp
  • G to T, chromosome 2 at 6,586,020 bp
  • A to G, chromosome 2 at 26,441,805 bp
  • T to G, chromosome 2 at 120,186,382 bp
  • A to T, chromosome 2 at 120,730,720 bp
  • T to A, chromosome 2 at 125,655,682 bp
  • G to A, chromosome 2 at 165,354,052 bp
  • T to C, chromosome 3 at 28,542,100 bp
  • A to T, chromosome 3 at 80,692,492 bp
  • A to T, chromosome 3 at 93,827,023 bp
  • A to G, chromosome 3 at 101,586,943 bp
  • T to C, chromosome 3 at 105,986,567 bp
  • A to G, chromosome 3 at 123,671,549 bp
  • T to A, chromosome 4 at 43,568,092 bp
  • G to A, chromosome 4 at 63,132,979 bp
  • G to A, chromosome 4 at 63,293,506 bp
  • T to C, chromosome 4 at 104,790,644 bp
  • A to G, chromosome 4 at 113,074,076 bp
  • G to A, chromosome 4 at 117,110,378 bp
  • T to C, chromosome 4 at 128,588,106 bp
  • T to A, chromosome 4 at 139,399,579 bp
  • G to A, chromosome 4 at 140,972,915 bp
  • T to A, chromosome 4 at 148,486,119 bp
  • A to T, chromosome 5 at 35,575,007 bp
  • G to A, chromosome 5 at 109,737,393 bp
  • C to T, chromosome 5 at 113,614,869 bp
  • G to A, chromosome 5 at 125,030,859 bp
  • G to T, chromosome 5 at 130,018,316 bp
  • A to G, chromosome 5 at 130,101,706 bp
  • G to A, chromosome 5 at 145,291,116 bp
  • A to G, chromosome 6 at 25,669,624 bp
  • T to C, chromosome 6 at 33,438,405 bp
  • A to T, chromosome 6 at 48,975,156 bp
  • T to C, chromosome 6 at 52,225,710 bp
  • T to C, chromosome 6 at 71,332,475 bp
  • C to A, chromosome 6 at 120,934,838 bp
  • C to T, chromosome 6 at 139,843,918 bp
  • C to A, chromosome 6 at 142,101,652 bp
  • T to C, chromosome 7 at 43,694,357 bp
  • T to A, chromosome 7 at 44,201,265 bp
  • T to C, chromosome 7 at 55,063,356 bp
  • T to C, chromosome 7 at 55,771,693 bp
  • A to G, chromosome 7 at 108,894,117 bp
  • T to A, chromosome 7 at 141,310,971 bp
  • AT to A, chromosome 8 at 71,407,207 bp
  • T to C, chromosome 8 at 85,053,803 bp
  • C to T, chromosome 8 at 87,688,081 bp
  • C to T, chromosome 8 at 89,187,002 bp
  • T to G, chromosome 8 at 107,048,535 bp
  • A to T, chromosome 8 at 109,876,883 bp
  • G to T, chromosome 8 at 112,773,739 bp
  • A to T, chromosome 8 at 119,535,347 bp
  • A to G, chromosome 9 at 20,814,896 bp
  • T to A, chromosome 9 at 37,898,326 bp
  • A to G, chromosome 9 at 45,150,439 bp
  • A to G, chromosome 9 at 99,875,662 bp
  • T to A, chromosome 9 at 111,362,261 bp
  • T to A, chromosome 10 at 21,145,324 bp
  • T to A, chromosome 10 at 77,550,050 bp
  • C to A, chromosome 10 at 81,364,235 bp
  • G to C, chromosome 10 at 122,802,312 bp
  • G to A, chromosome 10 at 129,268,405 bp
  • A to T, chromosome 11 at 11,801,268 bp
  • A to G, chromosome 11 at 58,920,357 bp
  • T to C, chromosome 11 at 67,926,421 bp
  • A to T, chromosome 11 at 74,206,535 bp
  • T to A, chromosome 11 at 83,283,314 bp
  • A to G, chromosome 11 at 87,856,925 bp
  • A to T, chromosome 11 at 100,590,977 bp
  • G to A, chromosome 11 at 105,348,431 bp
  • C to A, chromosome 12 at 8,011,331 bp
  • A to T, chromosome 12 at 35,997,541 bp
  • G to T, chromosome 12 at 102,400,124 bp
  • G to A, chromosome 12 at 105,713,575 bp
  • T to C, chromosome 12 at 107,916,530 bp
  • A to T, chromosome 13 at 21,573,574 bp
  • C to A, chromosome 13 at 96,946,482 bp
  • T to C, chromosome 14 at 20,520,646 bp
  • T to C, chromosome 14 at 24,452,508 bp
  • T to C, chromosome 14 at 27,500,420 bp
  • A to G, chromosome 14 at 30,689,790 bp
  • G to A, chromosome 14 at 32,274,864 bp
  • T to A, chromosome 14 at 47,726,179 bp
  • C to A, chromosome 14 at 50,127,073 bp
  • T to A, chromosome 14 at 51,826,075 bp
  • A to G, chromosome 14 at 52,313,753 bp
  • C to T, chromosome 14 at 56,028,592 bp
  • C to T, chromosome 14 at 70,402,197 bp
  • C to T, chromosome 15 at 6,787,144 bp
  • A to T, chromosome 15 at 9,306,400 bp
  • A to C, chromosome 15 at 10,592,138 bp
  • G to T, chromosome 16 at 88,729,254 bp
  • T to A, chromosome 17 at 30,684,237 bp
  • T to C, chromosome 17 at 34,379,725 bp
  • A to T, chromosome 17 at 52,602,836 bp
  • T to C, chromosome 18 at 34,318,565 bp
  • C to A, chromosome 18 at 44,276,401 bp
  • T to C, chromosome 18 at 48,047,703 bp
  • C to T, chromosome 18 at 71,548,998 bp
  • C to T, chromosome 18 at 76,136,847 bp
  • T to A, chromosome 19 at 12,674,954 bp
  • C to T, chromosome 19 at 13,480,012 bp
  • A to G, chromosome 19 at 45,001,612 bp
  • A to G, chromosome X at 73,774,941 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4611 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041822-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.