Strain Detail Sheet

Strain Name:
129-Hdctm1Ute/Mmucd
Stock Number:
041845-UCD
Citation ID:
RRID:MMRRC_041845-UCD
Other Names:
mHdc-lox-hygro-tk-lox, mHdc RMCE acceptor, IIG11

Gene Information

Gene Details

Hdctm1Ute
Name: histidine decarboxylase; targeted mutation 1, Ute Hochgeschwender
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Knock-In
Hdc
Name: histidine decarboxylase
Synonyms: Hdc-s, Hdc-e, Hdc-c, Hdc-a, L-histidine decarboxylase
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Knock-In
NCBI: 15186
HGNC: HGNC:4855
Homologene: 20490
Genetic Alterations
  • Murine Hdc gene (Gene ID:15186)
  • One endogenous mouse Hdc allele was replaced, via homologous recombination, by a lox-hygro/TS-lox cassette.
  • This line is a universal acceptor for recombinase-mediated cassette exchange for any newly identified HDC mutation.
Please also see related ES cell lines MMRRC:41842 ("IC6") and MMRRC:41846 ("IIG11") with associated information:
  • IIG11: one endogenous mouse Hdc allele was replaced, via homologous recombination, by a lox-hygro/TK-lox cassette.
  • IC6: hygro/TK cassette in IIG11 was replaced, via recombinase-mediated cassette exchange, by human HDC carrying the p.Trp317* nonsense mutation.
Genotype Determination
Genotyping protocol
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+

Strain Description

Strain of Origin
(129X1/SvJ x 129S1/Sv)F1-Kitl+

Strain Origin

Donor
Ute Hochgeschwender, M.D., Central Michigan University.
Nicole Calakos, M.D., Ph.D., Duke Institute for Brain Sciences.

Colony and Husbandry Information

Special Considerations

ES Cell Sex and Genotype: These ES Cells are expected to be male (XY) and heterozygous/hemizygous for the mutation.

Health Status Report

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment.

Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@ucdavis.edu.

Order Request Information

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
041845-UCD-CELL 129-Hdctm1Ute/Mmucd $1,192.87 / Non-Profit Vial Includes genetic confirmation
available service Chromosome counts $385.17 / Non-Profit per clone To determine the percentage of euploid metaphase chromosomes in the targeted ES cells. At least 20 spreads are counted.
additional products Extra vial of cells for the same clone $462.69 / Non-Profit per clone
available service Basic microinjection service $11,114.27 / Non-Profit per cell line In addition to the per mutant ES cell line fee (see above); chimeras not guaranteed. Injection of up to 3 clones.
additional products Feeder cells - Irradiated $148.67 / Non-Profit per cell line
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.