MMRRC:041917-MU

T to A, chromosome 1 at 11,065,825 bp
T to A, chromosome 1 at 16,769,418 bp
T to A, chromosome 1 at 20,364,167 bp
CTTTATTTTATTTTATTTTATTTTATTTTATTTTATTTTATT to CTTTATTTTATTTTATTTTATTTTATTTTATTTTATT, chromosome 1 at 40,327,310 bp
T to A, chromosome 1 at 74,925,354 bp
A to G, chromosome 1 at 87,125,834 bp
A to T, chromosome 1 at 93,625,656 bp
T to C, chromosome 1 at 131,753,138 bp
A to G, chromosome 1 at 150,624,550 bp
C to A, chromosome 1 at 173,760,361 bp
A to G, chromosome 1 at 173,907,660 bp
T to A, chromosome 2 at 24,224,404 bp
T to A, chromosome 2 at 36,734,403 bp
G to T, chromosome 2 at 59,001,888 bp
C to A, chromosome 2 at 69,466,993 bp
T to C, chromosome 2 at 70,238,899 bp
G to T, chromosome 2 at 93,956,576 bp
C to T, chromosome 2 at 104,433,759 bp
C to T, chromosome 2 at 119,775,006 bp
A to G, chromosome 2 at 143,070,973 bp
A to T, chromosome 2 at 144,536,336 bp
A to T, chromosome 2 at 151,230,764 bp
T to A, chromosome 2 at 162,933,427 bp
T to G, chromosome 3 at 86,737,164 bp
C to T, chromosome 3 at 113,153,477 bp
C to A, chromosome 3 at 133,234,681 bp
A to G, chromosome 3 at 154,256,584 bp
A to T, chromosome 4 at 43,984,981 bp
A to T, chromosome 4 at 48,051,522 bp
G to A, chromosome 4 at 65,314,796 bp
C to A, chromosome 4 at 111,891,744 bp
A to T, chromosome 4 at 118,397,669 bp
T to C, chromosome 4 at 123,117,133 bp
A to G, chromosome 4 at 145,074,842 bp
T to A, chromosome 5 at 4,197,176 bp
A to T, chromosome 5 at 24,718,231 bp
T to G, chromosome 5 at 33,901,813 bp
G to T, chromosome 5 at 41,818,612 bp
G to A, chromosome 5 at 52,582,920 bp
T to A, chromosome 5 at 130,231,774 bp
T to A, chromosome 5 at 148,352,399 bp
A to T, chromosome 6 at 17,281,410 bp
C to T, chromosome 6 at 71,329,774 bp
T to C, chromosome 6 at 123,232,196 bp
A to G, chromosome 7 at 16,907,146 bp
T to C, chromosome 7 at 112,149,046 bp
T to C, chromosome 7 at 119,457,168 bp
T to A, chromosome 7 at 119,640,694 bp
G to T, chromosome 7 at 126,374,302 bp
T to A, chromosome 7 at 142,303,754 bp
A to T, chromosome 8 at 33,335,991 bp
T to A, chromosome 8 at 36,994,391 bp
A to C, chromosome 8 at 84,102,838 bp
T to A, chromosome 8 at 104,615,226 bp
T to A, chromosome 8 at 123,134,575 bp
G to A, chromosome 9 at 14,866,983 bp
A to C, chromosome 9 at 20,300,623 bp
A to G, chromosome 9 at 32,322,677 bp
A to G, chromosome 9 at 46,269,872 bp
T to C, chromosome 9 at 59,875,416 bp
T to C, chromosome 9 at 72,611,692 bp
A to G, chromosome 9 at 124,476,821 bp
T to A, chromosome 10 at 7,705,684 bp
T to C, chromosome 10 at 8,725,660 bp
T to C, chromosome 10 at 75,511,125 bp
T to A, chromosome 10 at 80,233,014 bp
T to A, chromosome 10 at 81,367,552 bp
T to C, chromosome 10 at 84,992,137 bp
A to G, chromosome 10 at 128,353,137 bp
A to T, chromosome 11 at 17,946,964 bp
A to T, chromosome 11 at 29,805,108 bp
T to C, chromosome 11 at 53,465,588 bp
C to T, chromosome 11 at 58,948,971 bp
T to A, chromosome 11 at 59,042,290 bp
A to G, chromosome 11 at 65,005,452 bp
C to A, chromosome 11 at 83,709,869 bp
T to C, chromosome 11 at 87,814,347 bp
C to A, chromosome 11 at 95,277,598 bp
A to T, chromosome 11 at 120,013,478 bp
A to G, chromosome 11 at 120,444,498 bp
A to G, chromosome 12 at 4,713,197 bp
G to T, chromosome 12 at 72,094,049 bp
A to T, chromosome 12 at 113,762,267 bp
A to T, chromosome 12 at 118,192,427 bp
T to C, chromosome 13 at 21,183,760 bp
G to A, chromosome 13 at 40,015,388 bp
T to A, chromosome 13 at 75,132,235 bp
C to T, chromosome 13 at 81,405,364 bp
C to T, chromosome 14 at 50,950,871 bp
A to G, chromosome 14 at 54,643,047 bp
G to A, chromosome 15 at 44,547,347 bp
A to T, chromosome 16 at 20,313,072 bp
A to T, chromosome 16 at 49,172,058 bp
A to G, chromosome 16 at 49,762,594 bp
A to T, chromosome 16 at 78,547,926 bp
A to G, chromosome 16 at 94,262,698 bp
T to A, chromosome 17 at 13,073,617 bp
T to A, chromosome 17 at 27,890,105 bp
T to A, chromosome 17 at 31,108,434 bp
C to A, chromosome 17 at 32,800,923 bp
T to A, chromosome 17 at 35,442,759 bp
T to C, chromosome 17 at 65,852,691 bp
A to T, chromosome 17 at 83,450,948 bp
A to T, chromosome 17 at 83,832,345 bp
A to G, chromosome 18 at 37,448,847 bp
T to A, chromosome X at 97,341,633 bp

Genotype Determination

Genotyping Protocol(s)
Center protocol and contact for technical support will be shipped with mice.

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.

Strain Development

C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.

Suggested Control Mice

Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary

The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Strain Origin

Donor

Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Primary Reference

MUTAGENETIX record for R4657 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information Expand All

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

Appearance

Coat Color

Black

Breeding

MMRRC Breeding System

Random intra-strain mating

Breeding Scheme(s)

When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.

Overall Breeding Performance

Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size

Undetermined

Recommended wean age

3 weeks

Order Request Information Expand All

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution [Including applicable technology transfer agreements]

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description	Distribution Fee / Unit (US $) Shipping & Handling not included	Units	Notes
041917-MU-RESUS	Litter recovered from cryo-archive	$5,475.00 / Non-Profit	Litter	Recovered litter⁴; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

¹ The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

³ An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

⁴ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

Strain Detail Sheet

Strain Information Expand All

Colony and Husbandry Information Expand All

Order Request Information Expand All