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Strain Name:
C57BL/6J-MtgxR4701Btlr/Mmmh
Stock Number:
041949-MU
Citation ID:
RRID:MMRRC_041949-MU
Other Names:
R4701 (G1), C57BL/6J-MtgxR4701Btlr
Major Collection:

Strain Information

Tln2
Name: talin 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70549
VEGA: 9
Homologene: 56692
Lamb1
Name: laminin B1
Synonyms: Lamb-1, C81607, C80098, D130003D08Rik, Lamb1-1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16777
HGNC: HGNC:6486
Homologene: 1722
Otx1
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18423
HGNC: HGNC:8521
Homologene: 7875
Cadm1
Name: cell adhesion molecule 1
Synonyms: 3100001I08Rik, 2900073G06Rik, RA175N, RA175C, RA175B, RA175A, Necl2, SgIGSF, Tslc1, SynCam, Igsf4, Igsf4a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 54725
HGNC: HGNC:5951
Homologene: 8641
Kdm5b
Name: lysine demethylase 5B
Synonyms: PLU-1, 2010009J12Rik, Plu1, Rb-Bp2, 2210016I17Rik, D1Ertd202e, Jarid1b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 75605
Homologene: 48448
Arl15
Name: ADP-ribosylation factor-like 15
Synonyms: C230032K13Rik, Arfrp2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218639
Homologene: 56843
Fgfr1
Name: fibroblast growth factor receptor 1
Synonyms: Flt-2, Fgfr-1, Hspy, Eask, FGFR-I, Fr1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14182
HGNC: HGNC:3688
Homologene: 69065
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 73,995,204 bp
  • T to C, chromosome 1 at 74,918,908 bp
  • T to C, chromosome 1 at 88,234,612 bp
  • A to C, chromosome 1 at 88,241,618 bp
  • C to A, chromosome 1 at 92,597,438 bp
  • T to C, chromosome 1 at 93,078,835 bp
  • T to A, chromosome 1 at 134,606,012 bp
  • A to T, chromosome 1 at 160,147,768 bp
  • T to A, chromosome 1 at 182,892,061 bp
  • T to A, chromosome 2 at 3,322,641 bp
  • A to G, chromosome 2 at 11,718,345 bp
  • A to T, chromosome 2 at 82,256,582 bp
  • A to T, chromosome 2 at 113,584,071 bp
  • G to A, chromosome 2 at 120,705,713 bp
  • A to G, chromosome 2 at 126,414,293 bp
  • G to A, chromosome 2 at 130,667,878 bp
  • T to A, chromosome 2 at 137,094,456 bp
  • G to T, chromosome 2 at 153,950,385 bp
  • G to A, chromosome 2 at 180,191,696 bp
  • A to T, chromosome 3 at 63,699,496 bp
  • G to C, chromosome 3 at 94,391,710 bp
  • T to G, chromosome 3 at 106,732,077 bp
  • C to T, chromosome 3 at 127,598,704 bp
  • A to T, chromosome 4 at 82,820,334 bp
  • C to T, chromosome 4 at 104,731,751 bp
  • A to G, chromosome 4 at 139,471,336 bp
  • A to G, chromosome 4 at 141,199,809 bp
  • T to C, chromosome 4 at 152,496,659 bp
  • T to G, chromosome 5 at 109,427,983 bp
  • A to G, chromosome 5 at 115,103,313 bp
  • A to G, chromosome 5 at 123,322,613 bp
  • T to A, chromosome 5 at 127,564,496 bp
  • T to G, chromosome 5 at 142,185,231 bp
  • T to C, chromosome 6 at 14,718,993 bp
  • T to A, chromosome 6 at 23,075,856 bp
  • T to C, chromosome 6 at 32,516,688 bp
  • A to G, chromosome 6 at 64,665,915 bp
  • T to C, chromosome 6 at 104,804,360 bp
  • A to G, chromosome 6 at 118,506,485 bp
  • T to A, chromosome 6 at 123,650,469 bp
  • A to T, chromosome 6 at 138,150,838 bp
  • T to C, chromosome 6 at 146,861,942 bp
  • T to A, chromosome 7 at 30,876,153 bp
  • C to A, chromosome 7 at 42,465,093 bp
  • A to G, chromosome 7 at 45,517,924 bp
  • A to T, chromosome 7 at 46,731,627 bp
  • C to A, chromosome 7 at 56,255,002 bp
  • G to T, chromosome 7 at 64,243,500 bp
  • T to C, chromosome 7 at 79,839,465 bp
  • T to C, chromosome 7 at 96,895,349 bp
  • G to A, chromosome 7 at 103,683,062 bp
  • A to T, chromosome 7 at 104,860,649 bp
  • A to T, chromosome 7 at 105,035,591 bp
  • A to T, chromosome 7 at 105,056,879 bp
  • T to A, chromosome 7 at 133,916,462 bp
  • A to T, chromosome 7 at 141,357,260 bp
  • A to T, chromosome 8 at 22,000,121 bp
  • C to T, chromosome 8 at 25,557,759 bp
  • T to A, chromosome 8 at 71,473,810 bp
  • T to A, chromosome 8 at 89,031,160 bp
  • C to A, chromosome 8 at 105,390,429 bp
  • A to T, chromosome 9 at 15,270,360 bp
  • T to C, chromosome 9 at 20,212,625 bp
  • G to A, chromosome 9 at 47,818,822 bp
  • A to G, chromosome 9 at 56,620,258 bp
  • A to G, chromosome 9 at 59,323,519 bp
  • C to T, chromosome 9 at 64,897,357 bp
  • A to G, chromosome 9 at 67,346,527 bp
  • A to G, chromosome 9 at 80,314,400 bp
  • C to T, chromosome 9 at 104,095,565 bp
  • A to G, chromosome 10 at 10,550,854 bp
  • A to T, chromosome 10 at 24,893,648 bp
  • A to G, chromosome 10 at 50,720,664 bp
  • A to G, chromosome 11 at 9,292,306 bp
  • A to G, chromosome 11 at 21,711,719 bp
  • T to C, chromosome 11 at 21,999,459 bp
  • T to C, chromosome 11 at 49,626,808 bp
  • C to T, chromosome 11 at 50,863,010 bp
  • C to T, chromosome 11 at 77,817,665 bp
  • A to G, chromosome 11 at 93,965,908 bp
  • T to C, chromosome 11 at 101,411,160 bp
  • T to A, chromosome 12 at 31,266,848 bp
  • C to T, chromosome 12 at 31,447,774 bp
  • G to T, chromosome 12 at 116,440,618 bp
  • T to A, chromosome 13 at 113,967,725 bp
  • G to A, chromosome 14 at 36,938,697 bp
  • T to C, chromosome 15 at 7,237,776 bp
  • C to T, chromosome 15 at 39,086,708 bp
  • C to T, chromosome 15 at 76,708,585 bp
  • T to A, chromosome 15 at 80,257,417 bp
  • T to A, chromosome 15 at 99,241,945 bp
  • G to T, chromosome 16 at 5,212,193 bp
  • A to T, chromosome 16 at 32,755,846 bp
  • T to A, chromosome 16 at 33,456,908 bp
  • T to A, chromosome 16 at 58,677,969 bp
  • T to C, chromosome 17 at 33,433,935 bp
  • T to C, chromosome 17 at 34,199,411 bp
  • T to C, chromosome 17 at 35,096,606 bp
  • T to A, chromosome 17 at 55,496,344 bp
  • A to T, chromosome 17 at 55,784,971 bp
  • A to T, chromosome 17 at 80,543,652 bp
  • T to C, chromosome 18 at 35,266,896 bp
  • T to A, chromosome 19 at 8,852,055 bp
  • G to T, chromosome 19 at 30,004,495 bp
  • A to T, chromosome 19 at 38,725,007 bp
  • G to A, chromosome 19 at 42,607,613 bp
  • A to G, chromosome 19 at 47,884,656 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4701 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041949-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.