Strain Detail Sheet

Strain Name:
B6.129P2-Itgaltm1Mak/Mmjax
Stock Number:
042053-JAX
Citation ID:
RRID:MMRRC_042053-JAX
Other Names:
LFA-1, CD11a/CD18

Strain Information

Gene Details

Itgaltm1Mak
Name: integrin alpha L; targeted mutation 1, Tak W Mak
Synonyms: CD11a -, LFA-1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
Itgal
Name: integrin alpha L
Synonyms: LFA-1, Cd11a, Ly-21, Ly-15
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Knockout
NCBI: 16408
HGNC: HGNC:6148
Homologene: 1666
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Genetic Alterations
Itgal (LFA-1) knock-out mice exhibit defective leukocyte adhesion, reduced NK cytoxicity, absent DTH, and a selective defect in the induction of peripheral immune responses. LFA-1 exons 2, 3, 4, 5 and part of exon 6 are disrupted with a Neo insertion in reverse orientation. These mice may be useful for studies of lymphocyte activation and proliferation.
ES Cell Line
E14

Strain Description

Phenotype
The targeted Itgal (LFA-1) gene encodes the integrin alpha L chain and is involved in leukocyte intercellular adhesion and lymphocyte costimulator signaling. It is essential for cell-mediated cytotoxicity by cytotoxic T- lymphocytes (CTL) and natural killer (NK) cells. Mice homozygous for the knockout allele are viable and fertile, exhibiting no overt phenotype. Leukocytes from LFA-1 deficient mice fail to aggregate in response to PMA, indicating a failure of homotypic leukocyte adhesion. In addition, T cells exhibit a reduced proliferative capacity in mixed lymphocyte reaction (MLR) assays, NK cytotoxicity is reduced and delayed-type hypersensitivity (DTH) is absent. Although mice can mount a normal CTL response to LCMV and VSV infections, they fail to prime or reject injected tumor cells. These mice may be useful for studies of lymphocyte activation and proliferation.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
MeSH Terms
  • Animals
  • Base Sequence
  • CD18 Antigens/genetics
  • CD18 Antigens/immunology
  • Homozygote
  • Integrins/genetics
  • Integrins/immunology
  • Lymphocyte Function-Associated Antigen-1/genetics
  • Lymphocyte Function-Associated Antigen-1/immunology
  • Lymphocytic choriomeningitis virus/immunology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Molecular Sequence Data
  • Mutagenesis
  • T-Lymphocytes, Cytotoxic/immunology
  • Vesicular stomatitis Indiana virus/immunology
  • Virus Diseases/immunology
Strain Development
A targeting vector containing a neomycin cassette, in reverse orientation, was used to disrupt exons 2, 3, 4, 5, and part of exon 6. Exons 1-6 contain the signal peptide and the amino terminal extracellular region. The construct was electroporated into 129P2/OlaHsd derived E14K embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were bred to C57BL/6J to achieve germline transmission. The mice were then backcrossed to C57BL/6 for at least 8 generations. To establish a live colony, an aliquot of frozen sperm is used to fertilize C57BL/6J.
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Immunology and Inflammation

Strain Origin

Donor
Mak [Ph.D.], Tak
Primary Reference

Schmits R, Kundig TM, Baker DM, Shumaker G, Simard JJ, Duncan G, Wakeham A, Shahinian A, van der Heiden A, Bachmann MF, Ohashi PS, Mak TW, Hickstein DD. LFA-1-deficient mice show normal CTL responses to virus but fail to reject immunogenic tumor. J Exp Med. 1996 Apr 1;183(4):1415-26. (Medline PMID: 8666900)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
Black
Other
N/A

Breeding

MMRRC Breeding System
Random intra-strain mating
Generation
N8+
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 to 12 months 8 to 12 months
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.