Strain Detail Sheet

Strain Name:
B6.129P2-Itgaltm1Mak/Mmjax
Stock Number:
042053-JAX
Citation ID:
RRID:MMRRC_042053-JAX
Other Names:
LFA-1, CD11a/CD18

Strain Information

Gene Details

Itgaltm1Mak
Name: integrin alpha L; targeted mutation 1, Tak W Mak
Synonyms: CD11a -, LFA-1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
Itgal
Name: integrin alpha L
Synonyms: Ly-15, Cd11a, LFA-1, Ly-21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
NCBI: 16408
HGNC: HGNC:6148
Homologene: 1666
Genetic Alterations
LFA-1 exons 2, 3, 4, 5 and part of exon 6 deletion with Neo insertion in reverse orientation
ES Cell Line
E14

Strain Description

Phenotype

Homozygous: The leukocyte integrin LFA-1 (CD11a/CD18) plays an important role in lymphocyte recirculation and homotypic interactions. Leukocytes from mice lacking CD11a displayed defects in in vitro homotypic aggregation, in proliferation in mixed lymphocyte reactions, and in response to mitogen. Mutant mice mounted normal cytotoxic T cell (CTL) responses against systemic LCMV and VSV infections and showed normal ex vivo CTL function. However, LFA-1-deficient mice did not reject immunogenic tumors grafted into footpads and did not demonstrate priming response against tumor-specific antigen. Thus CD11a deficiency causes a selective defect in induction of peripheral immune responses whereas responses to systemic infection are normal.

Heterozygous: No phenotype

Cre-excised Phenotype: Undetermined

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
MeSH Terms
  • Animals
  • Base Sequence
  • CD18 Antigens/genetics
  • CD18 Antigens/immunology
  • Homozygote
  • Integrins/genetics
  • Integrins/immunology
  • Lymphocyte Function-Associated Antigen-1/genetics
  • Lymphocyte Function-Associated Antigen-1/immunology
  • Lymphocytic choriomeningitis virus/immunology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Molecular Sequence Data
  • Mutagenesis
  • T-Lymphocytes, Cytotoxic/immunology
  • Vesicular stomatitis Indiana virus/immunology
  • Virus Diseases/immunology
Strain Development
129 Ola ES cells were targeted by homologous recombination and then injected into C57BL/6 blastocysts. The resulting chimeras were backcrossed into C57BL/6J mice from Jackson (current stock number 000664)
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Immunology and Inflammation

Strain Origin

Donor
Mak [Ph.D.], Tak
Primary Reference

Schmits R, Kundig TM, Baker DM, Shumaker G, Simard JJ, Duncan G, Wakeham A, Shahinian A, van der Heiden A, Bachmann MF, Ohashi PS, Mak TW, Hickstein DD. LFA-1-deficient mice show normal CTL responses to virus but fail to reject immunogenic tumor. J Exp Med. 1996 Apr 1;183(4):1415-26. (Medline PMID: 8666900)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .

Appearance

Coat Color
Black
Other
N/A

Breeding

MMRRC Breeding System
Random intra-strain mating
Generation
N8+
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 to 12 months 8 to 12 months
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.


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