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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR4812Btlr/Mmmh
Stock Number:
042431-MU
Citation ID:
RRID:MMRRC_042431-MU
Other Names:
R4812 (G1), C57BL/6J-MtgxR4812Btlr
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Btrc
Name: beta-transducin repeat containing protein
Synonyms: SCF b-TRCP, beta-TrCP, Slimb, Beta-Trcp1, Fbw1a
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12234
VEGA: 19
HGNC: HGNC:1144
Homologene: 39330

Additional Resources
IMPC
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IMSR
Gene Ontology
BioGPS
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Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Ubn2
Name: ubinuclein 2
Synonyms: 6030408G03Rik, 2900060J04Rik, D130059P03Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320538
Homologene: 45564

Additional Resources
IMPC
Google
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PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Etv1
Name: ets variant 1
Synonyms: ER81, Etsrp81
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14009
HGNC: HGNC:3490
Homologene: 3636

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Rbms1
Name: RNA binding motif, single stranded interacting protein 1
Synonyms: YC1, MSSP-3, MSSP-2, MSSP-1, 2600014B10Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56878
HGNC: HGNC:9907
Homologene: 9640

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Kmt2e
Name: lysine (K)-specific methyltransferase 2E
Synonyms: 1810033J14Rik, D230038D11Rik, 9530077A04Rik, Mll5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69188
Homologene: 18822

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Tiparp
Name: TCDD-inducible poly(ADP-ribose) polymerase
Synonyms: DDF1, PARP-7, PARP7
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99929
Homologene: 9167

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Ctnna1
Name: catenin alpha 1
Synonyms: alpha(E)-catenin, alpha E catenin, Catna1, 2010010M04Rik, catenin (cadherin associated protein), alpha 1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12385
VEGA: 18
HGNC: HGNC:2509
Homologene: 1433

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 20,990,647 bp
  • C to T, chromosome 1 at 82,462,153 bp
  • T to A, chromosome 1 at 135,232,489 bp
  • A to G, chromosome 1 at 175,601,459 bp
  • T to A, chromosome 1 at 191,594,430 bp
  • T to C, chromosome 2 at 13,459,076 bp
  • C to T, chromosome 2 at 35,402,723 bp
  • A to C, chromosome 2 at 58,151,740 bp
  • C to T, chromosome 2 at 60,792,769 bp
  • T to A, chromosome 2 at 62,519,021 bp
  • C to T, chromosome 2 at 76,686,631 bp
  • A to G, chromosome 2 at 87,575,743 bp
  • T to A, chromosome 2 at 90,401,096 bp
  • C to T, chromosome 2 at 90,725,691 bp
  • T to A, chromosome 2 at 90,776,471 bp
  • T to C, chromosome 2 at 112,912,236 bp
  • T to A, chromosome 2 at 136,007,881 bp
  • A to G, chromosome 3 at 15,833,222 bp
  • A to C, chromosome 3 at 30,140,368 bp
  • C to T, chromosome 3 at 40,521,484 bp
  • T to C, chromosome 3 at 65,552,769 bp
  • T to A, chromosome 3 at 79,650,582 bp
  • A to T, chromosome 3 at 86,123,767 bp
  • C to T, chromosome 3 at 92,992,940 bp
  • G to A, chromosome 3 at 151,759,699 bp
  • A to G, chromosome 4 at 33,018,766 bp
  • C to T, chromosome 4 at 47,262,479 bp
  • A to G, chromosome 4 at 72,145,354 bp
  • C to T, chromosome 4 at 104,862,591 bp
  • A to G, chromosome 4 at 130,913,438 bp
  • T to C, chromosome 4 at 150,197,415 bp
  • C to T, chromosome 4 at 151,131,542 bp
  • A to G, chromosome 4 at 155,461,966 bp
  • T to A, chromosome 4 at 156,218,736 bp
  • A to G, chromosome 5 at 14,540,025 bp
  • G to A, chromosome 5 at 21,763,486 bp
  • T to C, chromosome 5 at 23,502,587 bp
  • T to C, chromosome 5 at 53,809,806 bp
  • T to C, chromosome 5 at 100,036,472 bp
  • A to G, chromosome 5 at 103,523,615 bp
  • T to C, chromosome 5 at 112,809,718 bp
  • A to G, chromosome 5 at 120,761,147 bp
  • G to A, chromosome 5 at 123,287,305 bp
  • T to G, chromosome 5 at 137,456,285 bp
  • C to A, chromosome 5 at 147,683,939 bp
  • G to T, chromosome 6 at 13,677,800 bp
  • A to T, chromosome 6 at 29,378,039 bp
  • T to A, chromosome 6 at 38,463,726 bp
  • T to C, chromosome 6 at 48,490,510 bp
  • T to A, chromosome 6 at 50,610,618 bp
  • A to C, chromosome 6 at 55,279,103 bp
  • T to A, chromosome 6 at 59,353,365 bp
  • A to T, chromosome 6 at 68,425,845 bp
  • A to T, chromosome 6 at 83,189,937 bp
  • T to C, chromosome 6 at 90,269,325 bp
  • C to T, chromosome 6 at 119,952,771 bp
  • A to G, chromosome 6 at 123,779,185 bp
  • A to G, chromosome 6 at 141,918,593 bp
  • G to A, chromosome 6 at 145,148,011 bp
  • G to A, chromosome 7 at 4,759,072 bp
  • A to G, chromosome 7 at 16,079,110 bp
  • A to C, chromosome 7 at 16,248,333 bp
  • C to T, chromosome 7 at 19,857,873 bp
  • C to G, chromosome 7 at 39,222,895 bp
  • A to T, chromosome 7 at 105,574,025 bp
  • T to A, chromosome 7 at 139,636,000 bp
  • G to A, chromosome 7 at 142,296,891 bp
  • A to G, chromosome 8 at 108,947,961 bp
  • A to G, chromosome 8 at 111,116,717 bp
  • T to A, chromosome 8 at 125,865,939 bp
  • C to T, chromosome 9 at 24,289,956 bp
  • A to G, chromosome 9 at 40,984,325 bp
  • T to C, chromosome 9 at 44,387,121 bp
  • A to T, chromosome 9 at 44,831,354 bp
  • A to G, chromosome 9 at 64,304,412 bp
  • C to T, chromosome 9 at 89,091,480 bp
  • A to G, chromosome 9 at 105,073,824 bp
  • T to C, chromosome 9 at 107,931,130 bp
  • T to C, chromosome 9 at 109,559,922 bp
  • T to G, chromosome 10 at 6,832,698 bp
  • G to A, chromosome 10 at 39,072,769 bp
  • C to A, chromosome 10 at 45,686,603 bp
  • A to G, chromosome 10 at 75,646,594 bp
  • A to G, chromosome 10 at 76,039,090 bp
  • A to G, chromosome 10 at 78,057,294 bp
  • T to C, chromosome 10 at 78,749,216 bp
  • A to T, chromosome 10 at 81,470,739 bp
  • A to G, chromosome 10 at 94,188,252 bp
  • T to A, chromosome 11 at 49,280,457 bp
  • T to C, chromosome 11 at 70,011,293 bp
  • T to A, chromosome 11 at 70,965,726 bp
  • T to C, chromosome 11 at 71,252,305 bp
  • T to A, chromosome 11 at 78,053,365 bp
  • T to C, chromosome 11 at 96,107,450 bp
  • T to C, chromosome 11 at 103,198,564 bp
  • T to C, chromosome 11 at 110,301,821 bp
  • T to A, chromosome 11 at 115,796,112 bp
  • A to T, chromosome 11 at 120,614,425 bp
  • T to G, chromosome 12 at 38,861,288 bp
  • C to T, chromosome 12 at 51,827,351 bp
  • T to A, chromosome 12 at 59,071,618 bp
  • T to C, chromosome 12 at 110,879,740 bp
  • G to A, chromosome 13 at 4,079,165 bp
  • T to A, chromosome 13 at 9,637,130 bp
  • T to A, chromosome 13 at 13,506,468 bp
  • T to G, chromosome 13 at 42,303,640 bp
  • T to A, chromosome 13 at 119,712,384 bp
  • T to A, chromosome 14 at 33,954,774 bp
  • A to G, chromosome 14 at 46,776,544 bp
  • T to C, chromosome 14 at 62,415,897 bp
  • T to G, chromosome 14 at 68,084,285 bp
  • T to G, chromosome 15 at 8,201,123 bp
  • A to T, chromosome 15 at 82,203,825 bp
  • A to G, chromosome 15 at 97,753,803 bp
  • A to T, chromosome 16 at 73,916,288 bp
  • G to A, chromosome 17 at 6,010,664 bp
  • C to T, chromosome 17 at 32,546,678 bp
  • T to A, chromosome 17 at 40,207,390 bp
  • G to A, chromosome 17 at 47,597,580 bp
  • A to G, chromosome 17 at 63,934,297 bp
  • T to A, chromosome 17 at 69,387,582 bp
  • T to G, chromosome 18 at 7,288,634 bp
  • T to A, chromosome 18 at 35,239,477 bp
  • T to A, chromosome 18 at 36,939,808 bp
  • A to T, chromosome 18 at 61,202,021 bp
  • T to A, chromosome 18 at 77,979,184 bp
  • G to A, chromosome 19 at 8,889,174 bp
  • A to G, chromosome 19 at 21,277,737 bp
  • A to G, chromosome 19 at 37,916,559 bp
  • A to T, chromosome 19 at 44,301,402 bp
  • G to A, chromosome 19 at 45,423,164 bp
  • A to G, chromosome X at 6,624,126 bp
  • T to A, chromosome Y at 2,106,334 bp
Genotype Determination

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4812 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
042431-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.