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Strain Name:
C57BL/6J-MtgxR4852Btlr/Mmmh
Stock Number:
042464-MU
Citation ID:
RRID:MMRRC_042464-MU
Other Names:
R4852 (G1), C57BL/6J-MtgxR4852Btlr
Major Collection:

Strain Information

Rubcn
Name: RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein
Synonyms: 1700021K19Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 100502698
Homologene: 15687
Nudc
Name: nudC nuclear distribution protein
Synonyms: NudC, Silg92
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18221
HGNC: HGNC:8045
Homologene: 4812
Mad1l1
Name: MAD1 mitotic arrest deficient 1-like 1
Synonyms: Mad1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17120
HGNC: HGNC:6762
Homologene: 74500
Usp7
Name: ubiquitin specific peptidase 7
Synonyms: 2210010O09Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 252870
Homologene: 2592
Chuk
Name: conserved helix-loop-helix ubiquitous kinase
Synonyms: Chuk1, IKK1, IKK[a], IKK-alpha, IkappaB kinase alpha, IKK 1, IKK alpha, IKKalpha, IKK-1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12675
HGNC: HGNC:1974
Homologene: 979
Ebag9
Name: estrogen receptor-binding fragment-associated gene 9
Synonyms: Rcas1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 55960
VEGA: 15
HGNC: HGNC:3123
Homologene: 3107
Gphn
Name: gephyrin
Synonyms: geph, 5730552E08Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 268566
VEGA: 12
Homologene: 10820
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 60,522,858 bp
  • A to T, chromosome 1 at 95,660,898 bp
  • G to A, chromosome 1 at 96,841,228 bp
  • A to G, chromosome 1 at 106,956,402 bp
  • T to C, chromosome 2 at 76,725,159 bp
  • T to A, chromosome 2 at 76,742,903 bp
  • T to C, chromosome 2 at 93,844,260 bp
  • T to C, chromosome 2 at 155,564,865 bp
  • T to C, chromosome 2 at 181,230,120 bp
  • T to A, chromosome 3 at 67,355,602 bp
  • C to A, chromosome 3 at 108,751,740 bp
  • T to C, chromosome 3 at 116,651,385 bp
  • T to A, chromosome 4 at 43,689,713 bp
  • C to T, chromosome 4 at 63,611,914 bp
  • A to G, chromosome 4 at 102,597,770 bp
  • A to G, chromosome 4 at 126,076,859 bp
  • A to G, chromosome 4 at 133,534,349 bp
  • C to T, chromosome 5 at 35,802,422 bp
  • A to T, chromosome 5 at 43,820,525 bp
  • T to A, chromosome 5 at 93,162,253 bp
  • T to C, chromosome 5 at 121,430,692 bp
  • T to C, chromosome 5 at 121,814,411 bp
  • C to T, chromosome 5 at 140,105,605 bp
  • C to T, chromosome 5 at 142,731,340 bp
  • T to A, chromosome 5 at 145,860,495 bp
  • A to T, chromosome 6 at 66,716,888 bp
  • A to G, chromosome 6 at 72,361,516 bp
  • T to C, chromosome 6 at 125,982,923 bp
  • A to T, chromosome 7 at 19,350,704 bp
  • G to A, chromosome 7 at 29,256,786 bp
  • A to G, chromosome 7 at 29,533,999 bp
  • G to A, chromosome 7 at 30,724,268 bp
  • A to G, chromosome 7 at 35,794,055 bp
  • A to T, chromosome 7 at 41,215,602 bp
  • A to G, chromosome 7 at 102,023,385 bp
  • A to T, chromosome 7 at 102,713,543 bp
  • A to G, chromosome 7 at 105,405,730 bp
  • G to A, chromosome 7 at 118,645,339 bp
  • A to T, chromosome 7 at 128,198,530 bp
  • T to C, chromosome 8 at 10,373,474 bp
  • T to C, chromosome 8 at 25,003,301 bp
  • A to G, chromosome 8 at 70,033,177 bp
  • A to T, chromosome 8 at 107,048,180 bp
  • A to T, chromosome 9 at 111,391,895 bp
  • A to G, chromosome 10 at 21,358,388 bp
  • T to A, chromosome 10 at 58,477,056 bp
  • CACTCGTCATACT to CACT, chromosome 10 at 89,430,958 bp
  • A to G, chromosome 10 at 94,298,549 bp
  • T to A, chromosome 11 at 4,702,198 bp
  • G to T, chromosome 11 at 4,891,468 bp
  • T to A, chromosome 11 at 62,211,684 bp
  • A to C, chromosome 11 at 64,989,404 bp
  • G to T, chromosome 11 at 67,224,278 bp
  • C to T, chromosome 11 at 69,884,376 bp
  • T to C, chromosome 11 at 73,814,248 bp
  • T to C, chromosome 11 at 76,785,150 bp
  • T to A, chromosome 11 at 102,146,621 bp
  • A to G, chromosome 11 at 110,244,203 bp
  • T to G, chromosome 12 at 32,965,471 bp
  • T to C, chromosome 12 at 35,996,123 bp
  • T to C, chromosome 12 at 53,104,675 bp
  • G to T, chromosome 12 at 71,164,546 bp
  • A to T, chromosome 12 at 71,164,547 bp
  • G to A, chromosome 12 at 78,627,210 bp
  • T to A, chromosome 12 at 103,173,466 bp
  • T to C, chromosome 12 at 113,140,584 bp
  • T to C, chromosome 13 at 38,634,984 bp
  • A to G, chromosome 13 at 55,012,596 bp
  • A to T, chromosome 13 at 55,161,156 bp
  • G to A, chromosome 13 at 74,199,645 bp
  • A to G, chromosome 13 at 100,161,536 bp
  • T to C, chromosome 13 at 114,820,841 bp
  • A to C, chromosome 13 at 119,788,858 bp
  • T to C, chromosome 15 at 44,624,519 bp
  • T to C, chromosome 15 at 50,846,309 bp
  • T to A, chromosome 15 at 53,886,038 bp
  • T to A, chromosome 15 at 78,904,338 bp
  • T to C, chromosome 15 at 80,388,479 bp
  • T to C, chromosome 15 at 100,645,288 bp
  • A to T, chromosome 16 at 8,756,844 bp
  • A to T, chromosome 16 at 32,843,308 bp
  • T to C, chromosome 17 at 3,178,219 bp
  • T to C, chromosome 17 at 31,528,069 bp
  • T to C, chromosome 17 at 32,415,849 bp
  • A to G, chromosome 17 at 33,937,173 bp
  • A to T, chromosome 17 at 68,559,753 bp
  • GCA to G, chromosome 17 at 73,351,506 bp
  • C to G, chromosome 18 at 6,623,487 bp
  • T to A, chromosome 18 at 6,623,488 bp
  • C to T, chromosome 18 at 37,322,471 bp
  • T to A, chromosome 18 at 37,692,050 bp
  • G to T, chromosome 18 at 37,956,568 bp
  • T to C, chromosome 18 at 43,577,400 bp
  • T to A, chromosome 18 at 63,777,949 bp
  • G to A, chromosome 19 at 4,143,791 bp
  • A to T, chromosome 19 at 44,088,758 bp
  • A to G, chromosome 19 at 47,897,111 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4852 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042464-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.